Dynamics of intra-hepatic lymphocytes in chronic hepatitis C: enrichment for Valpha24+ T cells and rapid elimination of effector cells by apoptosis - PubMed (original) (raw)
Dynamics of intra-hepatic lymphocytes in chronic hepatitis C: enrichment for Valpha24+ T cells and rapid elimination of effector cells by apoptosis
S Nuti et al. Eur J Immunol. 1998 Nov.
Free article
Abstract
Chronic viral hepatitis is characterized by a dramatic lymphocyte infiltrate in the liver. Although it is one of the most common chronic inflammatory diseases in humans, little information is available on the functional state of these intra-hepatic lymphocytes (IHL). To address this issue, we have optimized cytofluorimetric techniques to assess directly ex vivo the functions, dynamics and repertoires of IHL isolated from biopsies of patients with chronic hepatitis C. We estimate that 1% of the total body lymphocytes infiltrate the inflamed liver and find that, at variance with peripheral blood lymphocytes (PBL) isolated from the same patients, most IHL display an activated phenotype and produce Th1 type lymphokines when stimulated in vitro. Virtually all IHL are found in the G0/G1 state of the cell cycle, while a sizeable percentage of them is undergoing programmed cell death in vivo, as detected by the TUNEL assay performed on freshly isolated cells. In contrast again to PBL from the same patients, IHL show a preferential compartmentalization of NK and TCRgamma/delta+ cells, and a remarkable (up to 20-fold) enrichment for Valpha24+ T cells. Together our data suggest that in a liver injured by chronic hepatitis C, most IHL are pro-inflammatory activated cells which are highly enriched for effectors of innate resistance. These IHL do not undergo clonal expansion in the liver but rather display effector function and die in situ at a high rate, suggesting that maintenance of the IHL pool is dependent on continuous migration from extra-hepatic sites.
Similar articles
- Life, activation and death of intrahepatic lymphocytes in chronic hepatitis C.
Valiante NM, D'Andrea A, Crotta S, Lechner F, Klenerman P, Nuti S, Wack A, Abrignani S. Valiante NM, et al. Immunol Rev. 2000 Apr;174:77-89. doi: 10.1034/j.1600-0528.2002.017417.x. Immunol Rev. 2000. PMID: 10807508 Review. - T helper, cytotoxic T lymphocyte, NK cell and NK-T cell subpopulations in patients with chronic hepatitis C.
Amaraa R, Marecková H, Urbánek P, Fuciková T. Amaraa R, et al. Folia Microbiol (Praha). 2002;47(6):717-22. doi: 10.1007/BF02818677. Folia Microbiol (Praha). 2002. PMID: 12630325 - T lymphocytes infiltrating the liver during chronic hepatitis C infection express a broad range of T-cell receptor beta chain diversity.
Vigan I, Jouvin-Marche E, Leroy V, Pernollet M, Tongiani-Dashan S, Borel E, Delachanal E, Colomb M, Zarski JP, Marche PN. Vigan I, et al. J Hepatol. 2003 May;38(5):651-9. doi: 10.1016/s0168-8278(03)00089-8. J Hepatol. 2003. PMID: 12713877 - Lymphocyte distribution and intrahepatic compartmentalization during HCV infection: a main role for MHC-unrestricted T cells.
Agrati C, Nisii C, Oliva A, D'Offizi G, Montesano C, Pucillo LP, Poccia F. Agrati C, et al. Arch Immunol Ther Exp (Warsz). 2002;50(5):307-16. Arch Immunol Ther Exp (Warsz). 2002. PMID: 12455864 Review. - [The kinetic changes in murine lymphocyte subsets in regenerating liver].
Hirano F, Sakamoto T, Miki M, Yokomuro K. Hirano F, et al. Nihon Ika Daigaku Zasshi. 1992 Apr;59(2):166-75. doi: 10.1272/jnms1923.59.166. Nihon Ika Daigaku Zasshi. 1992. PMID: 1577919 Japanese.
Cited by
- Distinct cellular responses differentiating alcohol- and hepatitis C virus-induced liver cirrhosis.
Lederer SL, Walters KA, Proll S, Paeper B, Robinzon S, Boix L, Fausto N, Bruix J, Katze MG. Lederer SL, et al. Virol J. 2006 Nov 22;3:98. doi: 10.1186/1743-422X-3-98. Virol J. 2006. PMID: 17121680 Free PMC article. - Human natural killer T cells are heterogeneous in their capacity to reprogram their effector functions.
Eger KA, Sundrud MS, Motsinger AA, Tseng M, Van Kaer L, Unutmaz D. Eger KA, et al. PLoS One. 2006 Dec 20;1(1):e50. doi: 10.1371/journal.pone.0000050. PLoS One. 2006. PMID: 17183680 Free PMC article. - Simultaneous evaluation of lymphocyte subpopulations in the liver and in peripheral blood mononuclear cells of HCV-infected patients: relationship with histological lesions.
Pernollet M, Jouvin-Marche E, Leroy V, Vigan I, Zarski JP, Marche PN. Pernollet M, et al. Clin Exp Immunol. 2002 Dec;130(3):518-25. doi: 10.1046/j.1365-2249.2002.01996.x. Clin Exp Immunol. 2002. PMID: 12452844 Free PMC article. - Apoptosis-inducing protein derived from hepatocyte selectively induces apoptosis in lymphocytes.
Ikeda M, Yoshikawa H, Liu J, Nakajima Y, Akahane Y, Tasaka K. Ikeda M, et al. Immunology. 2003 Jan;108(1):116-22. doi: 10.1046/j.1365-2567.2003.01542.x. Immunology. 2003. PMID: 12519310 Free PMC article. - Psychosocial stress and liver disease status.
Vere CC, Streba CT, Streba LM, Ionescu AG, Sima F. Vere CC, et al. World J Gastroenterol. 2009 Jun 28;15(24):2980-6. doi: 10.3748/wjg.15.2980. World J Gastroenterol. 2009. PMID: 19554650 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources