Differential expression of peroxisome proliferator-activated receptor subtypes during the differentiation of human keratinocytes - PubMed (original) (raw)

Comparative Study

. 1998 Dec;111(6):1116-21.

doi: 10.1046/j.1523-1747.1998.00439.x.

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Comparative Study

Differential expression of peroxisome proliferator-activated receptor subtypes during the differentiation of human keratinocytes

M Rivier et al. J Invest Dermatol. 1998 Dec.

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Abstract

The expression of mRNA encoding peroxisome proliferator-activated receptor (PPAR) subtypes in human keratinocytes was determined by semiquantitative reverse transcriptase-polymerase chain reaction. When normal human keratinocytes were induced to differentiate by shifting the culture medium to high Ca2+ concentration, the expression of PPAR-alpha and -gamma mRNA was increased, whereas that of PPAR-delta remained unchanged. At the protein level, the expression of PPAR in cultured human keratinocytes was demonstrated by a DNA mobility shift assay and the functionality of the receptor subtypes was assessed by transactivation experiments. In epidermis reconstructed in vitro, the level of PPAR-alpha and -gamma mRNA was also associated with keratinocyte differentiation. In lesional compared with nonlesional psoriatic epidermis, the expression of PPAR-alpha and -gamma mRNA was reduced, indicating that these two subtypes are tightly linked to the epidermal differentiation process.

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