Identification of reggie-1 and reggie-2 as plasmamembrane-associated proteins which cocluster with activated GPI-anchored cell adhesion molecules in non-caveolar micropatches in neurons - PubMed (original) (raw)
Identification of reggie-1 and reggie-2 as plasmamembrane-associated proteins which cocluster with activated GPI-anchored cell adhesion molecules in non-caveolar micropatches in neurons
D M Lang et al. J Neurobiol. 1998 Dec.
Abstract
Neurons are believed to possess plasmalemmal microdomains and proteins analogous to the caveolae and caveolin of nonneuronal cells. Caveolae are plasmalemmal invaginations where activated glycosyl-phosphatidylinositol (GPI)-anchored proteins preferentially assemble and where transmembrane signaling may occur. Molecular cloning of rat reggie-1 and -2 (80% identical to goldfish reggie proteins) shows that reggie-2 is practically identical to mouse flotillin-1. Flotillin-1 and epidermal surface antigen (ESA) (flotillin-2) are suggested to represent possible membrane proteins in caveolae. Rat reggie-1 is 99% homologous to ESA in overlapping sequences but has a 49-amino-acid N-terminus not present in ESA. Antibodies (ABs) which recognize reggie-1 or -2 reveal that both proteins cluster at the plasmamembrane and occur in micropatches in neurons [dorsal root ganglia (DRGs), retinal ganglion, and PC-12 cells] and in nonneuronal cells. In neurons, reggie micropatches occur along the axon and in lamellipodia and filopodia of growth cones, but they do not occur in caveolae. By quantitative electronmicroscopic analysis we demonstrate the absence of caveolae in (anti-caveolin negative) neurons and show anti-reggie-1 immunogold-labeled clusters at the plasmamembrane of DRGs. When ABs against the GPI-anchored cell adhesion molecules (CAMs) F3 and Thy-1 are applied to live DRGs, the GPI-linked CAMs sequester into micropatches. Double immunofluorescence shows a colocalization of the CAMs with micropatches of anti-reggie antibodies. Thus, reggie-1 and reggie-2 identify sites where activated GPI-linked CAMs preferentially accumulate and which may represent noncaveolar micropatches (domains).
Similar articles
- Glycosylphosphatidyl inositol-anchored proteins and fyn kinase assemble in noncaveolar plasma membrane microdomains defined by reggie-1 and -2.
Stuermer CA, Lang DM, Kirsch F, Wiechers M, Deininger SO, Plattner H. Stuermer CA, et al. Mol Biol Cell. 2001 Oct;12(10):3031-45. doi: 10.1091/mbc.12.10.3031. Mol Biol Cell. 2001. PMID: 11598189 Free PMC article. - Flotillin 2 is distinct from epidermal surface antigen (ESA) and is associated with filopodia formation.
Hazarika P, Dham N, Patel P, Cho M, Weidner D, Goldsmith L, Duvic M. Hazarika P, et al. J Cell Biochem. 1999 Oct 1;75(1):147-59. J Cell Biochem. 1999. PMID: 10462713 - Reggie-1 and reggie-2, two cell surface proteins expressed by retinal ganglion cells during axon regeneration.
Schulte T, Paschke KA, Laessing U, Lottspeich F, Stuermer CA. Schulte T, et al. Development. 1997 Jan;124(2):577-87. doi: 10.1242/dev.124.2.577. Development. 1997. PMID: 9053333 - Scaffolding microdomains and beyond: the function of reggie/flotillin proteins.
Langhorst MF, Reuter A, Stuermer CA. Langhorst MF, et al. Cell Mol Life Sci. 2005 Oct;62(19-20):2228-40. doi: 10.1007/s00018-005-5166-4. Cell Mol Life Sci. 2005. PMID: 16091845 Free PMC article. Review. - Reggie/flotillin and the targeted delivery of cargo.
Stuermer CA. Stuermer CA. J Neurochem. 2011 Mar;116(5):708-13. doi: 10.1111/j.1471-4159.2010.07007.x. Epub 2011 Jan 7. J Neurochem. 2011. PMID: 21214550 Review.
Cited by
- Physiological and pathological roles of caveolins in the central nervous system.
Badaut J, Blochet C, Obenaus A, Hirt L. Badaut J, et al. Trends Neurosci. 2024 Aug;47(8):651-664. doi: 10.1016/j.tins.2024.06.003. Epub 2024 Jul 6. Trends Neurosci. 2024. PMID: 38972795 Free PMC article. Review. - LUBAC-mediated M1 Ub regulates necroptosis by segregating the cellular distribution of active MLKL.
Weinelt N, Wächtershäuser KN, Celik G, Jeiler B, Gollin I, Zein L, Smith S, Andrieux G, Das T, Roedig J, Feist L, Rotter B, Boerries M, Pampaloni F, van Wijk SJL. Weinelt N, et al. Cell Death Dis. 2024 Jan 20;15(1):77. doi: 10.1038/s41419-024-06447-6. Cell Death Dis. 2024. PMID: 38245534 Free PMC article. - Are There Lipid Membrane-Domain Subtypes in Neurons with Different Roles in Calcium Signaling?
Samhan-Arias AK, Poejo J, Marques-da-Silva D, Martínez-Costa OH, Gutierrez-Merino C. Samhan-Arias AK, et al. Molecules. 2023 Dec 2;28(23):7909. doi: 10.3390/molecules28237909. Molecules. 2023. PMID: 38067638 Free PMC article. Review. - Unraveling the Cave: A Seventy-Year Journey into the Caveolar Network, Cellular Signaling, and Human Disease.
D'Alessio A. D'Alessio A. Cells. 2023 Nov 22;12(23):2680. doi: 10.3390/cells12232680. Cells. 2023. PMID: 38067108 Free PMC article. Review. - The scaffolding protein flot2 promotes cytoneme-based transport of wnt3 in gastric cancer.
Routledge D, Rogers S, Ono Y, Brunt L, Meniel V, Tornillo G, Ashktorab H, Phesse TJ, Scholpp S. Routledge D, et al. Elife. 2022 Aug 30;11:e77376. doi: 10.7554/eLife.77376. Elife. 2022. PMID: 36040316 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous