Importance of the intracytoplasmic domain of the simian immunodeficiency virus (SIV) envelope glycoprotein for pathogenesis - PubMed (original) (raw)
. 1998 Dec 5;252(1):9-16.
doi: 10.1006/viro.1998.9467.
Affiliations
- PMID: 9875311
- DOI: 10.1006/viro.1998.9467
Free article
Importance of the intracytoplasmic domain of the simian immunodeficiency virus (SIV) envelope glycoprotein for pathogenesis
P A Luciw et al. Virology. 1998.
Free article
Abstract
SIVmac1A11 and SIVmac239 are nonpathogenic and pathogenic molecular clones in rhesus macaques, respectively. Although these viruses exhibit approximately 98% nucleotide and amino acid sequence homology, differences are found in the length of the translation frames for several genes. SIVmac239 has a premature stop codon in nef, whereas SIVmac1A11 has a premature stop codon in vpr and two premature stop codons in the intracytoplasmic domain of the env-transmembrane (TM) subunit. Recombinant viruses, constructed through reciprocal exchange of large DNA restriction enzyme fragments between SIVmac1A11 and SIVmac239, were evaluated in adult rhesus macaques. This in vivo analysis revealed that two or more regions of the SIVmac genome were essential for high virus load and disease progression (Marthas et al., 1993. J. Virol. 67, 6047-6055). An important gap in knowledge remaining from this study was whether the premature stop codons in env-TM of recombinant virus SIV1A11/239gag-env/1A11 (Full-length vpr and nef, two stop codons in env-TM) reverted to coding triplets in vivo. Here, we report that viral sequences in macaques, which succumbed to an AIDS-like disease after infection with SIV1A11/239gag-env/1A11, exhibited reversion of both env-TM stop codons. In addition, antibodies to the intracytoplasmic domain of env-TM were detected in macaques containing revertant virus and showing disease; this finding indicates that this domain of the env glycoprotein was expressed in vivo. Thus selection for viral variants with full-length env-TM demonstrated that the cytoplasmic domain of the SIVmac env glycoprotein plays a role in viral persistence and immunodeficiency in primates.
Similar articles
- Chronology of genetic changes in the vpu, env, and Nef genes of chimeric simian-human immunodeficiency virus (strain HXB2) during acquisition of virulence for pig-tailed macaques.
McCormick-Davis C, Zhao LJ, Mukherjee S, Leung K, Sheffer D, Joag SV, Narayan O, Stephens EB. McCormick-Davis C, et al. Virology. 1998 Sep 1;248(2):275-83. doi: 10.1006/viro.1998.9300. Virology. 1998. PMID: 9721236 - Viral determinants of simian immunodeficiency virus (SIV) virulence in rhesus macaques assessed by using attenuated and pathogenic molecular clones of SIVmac.
Marthas ML, Ramos RA, Lohman BL, Van Rompay KK, Unger RE, Miller CJ, Banapour B, Pedersen NC, Luciw PA. Marthas ML, et al. J Virol. 1993 Oct;67(10):6047-55. doi: 10.1128/JVI.67.10.6047-6055.1993. J Virol. 1993. PMID: 8371353 Free PMC article. - The intracytoplasmic domain of the Env transmembrane protein is a locus for attenuation of simian immunodeficiency virus SIVmac in rhesus macaques.
Shacklett BL, Weber CJ, Shaw KE, Keddie EM, Gardner MB, Sonigo P, Luciw PA. Shacklett BL, et al. J Virol. 2000 Jul;74(13):5836-44. doi: 10.1128/jvi.74.13.5836-5844.2000. J Virol. 2000. PMID: 10846063 Free PMC article. - Genetic and biological comparisons of pathogenic and nonpathogenic molecular clones of simian immunodeficiency virus (SIVmac).
Luciw PA, Shaw KE, Unger RE, Planelles V, Stout MW, Lackner JE, Pratt-Lowe E, Leung NJ, Banapour B, Marthas ML. Luciw PA, et al. AIDS Res Hum Retroviruses. 1992 Mar;8(3):395-402. doi: 10.1089/aid.1992.8.395. AIDS Res Hum Retroviruses. 1992. PMID: 1571198 Review. - Nonhuman primate lentiviruses: models for human infection.
Kodama T, Kestler HW 3rd, Burns DP, Ringler DJ, King NW, Daniel MD, Desrosiers RC. Kodama T, et al. Dev Biol Stand. 1990;72:267-71. Dev Biol Stand. 1990. PMID: 2282986 Review. No abstract available.
Cited by
- In vivo attenuation of simian immunodeficiency virus by disruption of a tyrosine-dependent sorting signal in the envelope glycoprotein cytoplasmic tail.
Fultz PN, Vance PJ, Endres MJ, Tao B, Dvorin JD, Davis IC, Lifson JD, Montefiori DC, Marsh M, Malim MH, Hoxie JA. Fultz PN, et al. J Virol. 2001 Jan;75(1):278-91. doi: 10.1128/JVI.75.1.278-291.2001. J Virol. 2001. PMID: 11119598 Free PMC article. - Modulation of Env content in virions of simian immunodeficiency virus: correlation with cell surface expression and virion infectivity.
Yuste E, Reeves JD, Doms RW, Desrosiers RC. Yuste E, et al. J Virol. 2004 Jul;78(13):6775-85. doi: 10.1128/JVI.78.13.6775-6785.2004. J Virol. 2004. PMID: 15194752 Free PMC article. - Virion envelope content, infectivity, and neutralization sensitivity of simian immunodeficiency virus.
Yuste E, Johnson W, Pavlakis GN, Desrosiers RC. Yuste E, et al. J Virol. 2005 Oct;79(19):12455-63. doi: 10.1128/JVI.79.19.12455-12463.2005. J Virol. 2005. PMID: 16160173 Free PMC article. - Identification of two sequences in the cytoplasmic tail of the human immunodeficiency virus type 1 envelope glycoprotein that inhibit cell surface expression.
Bültmann A, Muranyi W, Seed B, Haas J. Bültmann A, et al. J Virol. 2001 Jun;75(11):5263-76. doi: 10.1128/JVI.75.11.5263-5276.2001. J Virol. 2001. PMID: 11333908 Free PMC article. - HIV-1 envelope glycoprotein biosynthesis, trafficking, and incorporation.
Checkley MA, Luttge BG, Freed EO. Checkley MA, et al. J Mol Biol. 2011 Jul 22;410(4):582-608. doi: 10.1016/j.jmb.2011.04.042. J Mol Biol. 2011. PMID: 21762802 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous