A general method for isolation of high molecular weight DNA from eukaryotes - PubMed (original) (raw)

A general method for isolation of high molecular weight DNA from eukaryotes

N Blin et al. Nucleic Acids Res. 1976 Sep.

Free PMC article

Abstract

A new method for isolation of high molecular weight DNA from eukaryotes is presented. This procedure allows preparation of DNA from a variety of tissues such as calf thymus or human placenta and from cells which were more difficult to lyse until now (e.g. Crypthecodinium cuhnii, a dinoflagellate). The DNA obtained in such a way has an average molecular weight of about 200 X 10(6) d and contains very few, if any, single strand breaks.

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References

1. 1. J Mol Biol. 1965 Feb;11:373-90 - PubMed
2. 1. Biophys J. 1963 Jul;3:309-21 - PubMed
3. 1. Proc Natl Acad Sci U S A. 1974 May;71(5):2108-12 - PubMed
4. 1. Eur J Biochem. 1973 Jul 2;36(1):32-8 - PubMed
5. 1. J Mol Biol. 1972 Jun 14;67(1):11-24 - PubMed

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