TdT-accessible breaks are scattered over the immunoglobulin V domain in a constitutively hypermutating B cell line - PubMed (original) (raw)
TdT-accessible breaks are scattered over the immunoglobulin V domain in a constitutively hypermutating B cell line
J E Sale et al. Immunity. 1998 Dec.
Free article
Abstract
Searching for an in vitro model for somatic hypermutation, we have identified an IgM-expressing Burkitt lymphoma line that constitutively diversifies its immunoglobulin V domain at high rate during culture. As in in vivo, the mutations are largely nucleotide substitutions with the pattern of substitutions revealing a component of the human hypermutation program that is preferentially targeted to G/C residues. The substitutions frequently create stop codons with IgM-loss variants also being generated by V domain-specific deletions and duplications. However, in transfectants expressing terminal deoxynucleotidyl transferase, many IgM-loss variants additionally arise through short nontemplated nucleotide insertions into the V (but not C) domain. Thus, antibody hypermutation is likely accompanied by DNA strand breaks scattered within the mutation domain.
Similar articles
- ATM is not required in somatic hypermutation of VH, but is involved in the introduction of mutations in the switch mu region.
Pan-Hammarström Q, Dai S, Zhao Y, van Dijk-Härd IF, Gatti RA, Børresen-Dale AL, Hammarström L. Pan-Hammarström Q, et al. J Immunol. 2003 Apr 1;170(7):3707-16. doi: 10.4049/jimmunol.170.7.3707. J Immunol. 2003. PMID: 12646636 - Extensive junctional diversity in Ig light chain genes from early B cell progenitors of mu MT mice.
Bentolila LA, Olson S, Marshall A, Rougeon F, Paige CJ, Doyen N, Wu GE. Bentolila LA, et al. J Immunol. 1999 Feb 15;162(4):2123-8. J Immunol. 1999. PMID: 9973486 - Scope of action of the immunoglobulin mutator system.
Wabl MR, Jäck HM, von Borstel RC, Steinberg CM. Wabl MR, et al. Genome. 1989;31(1):118-21. doi: 10.1139/g89-022. Genome. 1989. PMID: 2512197 Review. - Mapping of the genes for mouse immunoglobulins M and D.
Mushinski JF. Mushinski JF. Crit Rev Immunol. 1983;4(2):169-95. Crit Rev Immunol. 1983. PMID: 6406143 Review. No abstract available.
Cited by
- Mechanism and regulation of secondary immunoglobulin diversification.
Bello A, Hirth G, Voigt S, Tepper S, Jungnickel B. Bello A, et al. Cell Cycle. 2023 Sep;22(18):2070-2087. doi: 10.1080/15384101.2023.2275397. Epub 2023 Nov 23. Cell Cycle. 2023. PMID: 37909747 Review. - HMCES protects immunoglobulin genes specifically from deletions during somatic hypermutation.
Wu L, Shukla V, Yadavalli AD, Dinesh RK, Xu D, Rao A, Schatz DG. Wu L, et al. Genes Dev. 2022 Apr 1;36(7-8):433-450. doi: 10.1101/gad.349438.122. Epub 2022 Apr 21. Genes Dev. 2022. PMID: 35450882 Free PMC article. - Expression of Constitutive Fusion of Ubiquitin to PCNA Restores the Level of Immunoglobulin A/T Mutations During Somatic Hypermutation in the Ramos Cell Line.
Lerner LK, Bonte D, Le Guillou M, Mohammad MM, Kasraian Z, Sarasin A, Despras E, Aoufouchi S. Lerner LK, et al. Front Immunol. 2022 Apr 1;13:871766. doi: 10.3389/fimmu.2022.871766. eCollection 2022. Front Immunol. 2022. PMID: 35432321 Free PMC article. - The role of HIRA-dependent H3.3 deposition and its modifications in the somatic hypermutation of immunoglobulin variable regions.
Yu G, Zhang Y, Gupta V, Zhang J, MacCarthy T, Duan Z, Scharff MD. Yu G, et al. Proc Natl Acad Sci U S A. 2021 Dec 14;118(50):e2114743118. doi: 10.1073/pnas.2114743118. Proc Natl Acad Sci U S A. 2021. PMID: 34873043 Free PMC article. - A Bayesian model based computational analysis of the relationship between bisulfite accessible single-stranded DNA in chromatin and somatic hypermutation of immunoglobulin genes.
Yu G, Wu Y, Duan Z, Tang C, Xing H, Scharff MD, MacCarthy T. Yu G, et al. PLoS Comput Biol. 2021 Sep 7;17(9):e1009323. doi: 10.1371/journal.pcbi.1009323. eCollection 2021 Sep. PLoS Comput Biol. 2021. PMID: 34491985 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous