Molecular mapping of chromosome 2 deletions in murine radiation-induced AML localizes a putative tumor suppressor gene to a 1.0 cM region homologous to human chromosome segment 11p11-12 - PubMed (original) (raw)
Affiliations
- PMID: 9885975
Molecular mapping of chromosome 2 deletions in murine radiation-induced AML localizes a putative tumor suppressor gene to a 1.0 cM region homologous to human chromosome segment 11p11-12
A Silver et al. Genes Chromosomes Cancer. 1999 Feb.
Abstract
Radiation-induced acute myeloid leukemias (AMLs) in the mouse are characterized by chromosome 2 deletions. Previous studies showed that a minimal deleted region (mdr) of approximately 6.5 cM is lost from one homologue in chromosome 2-deleted AMLs. An AML tumor suppressor gene is proposed to map within this mdr. In this study, we refine the mdr to a I cM interval between markers D2Mit126 and D2Mit185 by microsatellite analysis of 21 primary radiation-induced F I AMLs. The construction of a partial yeast artificial chromosome (YAC) contig spanning the mdr and the location of six known genes indicated that the 1 cM mdr is homologous to human 11p11-12, a region implicated in some human AMLs. Screening of five cell lines derived from primary radiation-induced AMLs for homozygous loss of microsatellites and genes mapping within the mdr revealed loss of both copies of the hemopoietic tissue-specific transcription factor Sfpi1(PU.1/Spi1) in one cell line. Studies of primary and F1 AMLs failed to implicate Sfpi1 as the AML tumor suppressor gene. YAC contig construction, together with data suggesting that the critical gene flanks Sfpi1, represents significant progress toward identifying an AML tumor suppressor gene.
Similar articles
- Functional evidence from microcell-mediated chromosome transfer of myeloid leukemia suppressor genes on human chromosomes 7 and 11.
Wilding J, Meijne E, Haines J, Moody J, Edwards A, Newbold RF, Parris C, Cox R, Silver A. Wilding J, et al. Genes Chromosomes Cancer. 2002 Aug;34(4):390-7. doi: 10.1002/gcc.10086. Genes Chromosomes Cancer. 2002. PMID: 12112528 - Microsatellite analysis of recurrent chromosome 2 deletions in acute myeloid leukaemia induced by radiation in F1 hybrid mice.
Clark DJ, Meijne EI, Bouffler SD, Huiskamp R, Skidmore CJ, Cox R, Silver AR. Clark DJ, et al. Genes Chromosomes Cancer. 1996 Aug;16(4):238-46. doi: 10.1002/(SICI)1098-2264(199608)16:4<238::AID-GCC3>3.0.CO;2-Z. Genes Chromosomes Cancer. 1996. PMID: 8875237 - Defining a common region of deletion at 13q21 in human cancers.
Chen C, Brabham WW, Stultz BG, Frierson HF Jr, Barrett JC, Sawyers CL, Isaacs JT, Dong JT. Chen C, et al. Genes Chromosomes Cancer. 2001 Aug;31(4):333-44. doi: 10.1002/gcc.1152. Genes Chromosomes Cancer. 2001. PMID: 11433524 - Chromosomal deletions in AML.
Nagarajan L. Nagarajan L. Cancer Treat Res. 2010;145:59-66. doi: 10.1007/978-0-387-69259-3_4. Cancer Treat Res. 2010. PMID: 20306245 Review. - PU.1 downregulation in murine radiation-induced acute myeloid leukaemia (AML): from molecular mechanism to human AML.
Verbiest T, Bouffler S, Nutt SL, Badie C. Verbiest T, et al. Carcinogenesis. 2015 Apr;36(4):413-9. doi: 10.1093/carcin/bgv016. Epub 2015 Mar 6. Carcinogenesis. 2015. PMID: 25750172 Free PMC article. Review.
Cited by
- Spi1 R235C point mutation confers hypersensitivity to radiation-induced acute myeloid leukemia in mice.
Brown N, Finnon R, Finnon P, McCarron R, Cruz-Garcia L, O'Brien G, Herbert E, Scudamore CL, Morel E, Badie C. Brown N, et al. iScience. 2023 Aug 3;26(9):107530. doi: 10.1016/j.isci.2023.107530. eCollection 2023 Sep 15. iScience. 2023. PMID: 37664628 Free PMC article. - Hyper-radiosensitivity affects low-dose acute myeloid leukemia incidence in a mathematical model.
Stouten S, Balkenende B, Roobol L, Lunel SV, Badie C, Dekkers F. Stouten S, et al. Radiat Environ Biophys. 2022 Aug;61(3):361-373. doi: 10.1007/s00411-022-00981-7. Epub 2022 Jul 21. Radiat Environ Biophys. 2022. PMID: 35864346 Free PMC article. - Oxidative Stress and X-ray Exposure Levels-Dependent Survival and Metabolic Changes in Murine HSPCs.
Karabulutoglu M, Finnon R, Cruz-Garcia L, Hill MA, Badie C. Karabulutoglu M, et al. Antioxidants (Basel). 2021 Dec 22;11(1):11. doi: 10.3390/antiox11010011. Antioxidants (Basel). 2021. PMID: 35052515 Free PMC article. - CircIMMP2L promotes esophageal squamous cell carcinoma malignant progression via CtBP1 nuclear retention dependent epigenetic modification.
Liang Y, Mao Q, Wang L, Xia W, Chen B, Wang H, Li R, Xu L, Jiang F, Dong G. Liang Y, et al. Clin Transl Med. 2021 Sep;11(9):e519. doi: 10.1002/ctm2.519. Clin Transl Med. 2021. PMID: 34586741 Free PMC article. - Modeling low-dose radiation-induced acute myeloid leukemia in male CBA/H mice.
Stouten S, Verduyn Lunel S, Finnon R, Badie C, Dekkers F. Stouten S, et al. Radiat Environ Biophys. 2021 Mar;60(1):49-60. doi: 10.1007/s00411-020-00880-9. Epub 2020 Nov 22. Radiat Environ Biophys. 2021. PMID: 33221961 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials