Vaccination with live Escherichia coli expressing Brucella abortus Cu/Zn superoxide dismutase protects mice against virulent B. abortus - PubMed (original) (raw)

Vaccination with live Escherichia coli expressing Brucella abortus Cu/Zn superoxide dismutase protects mice against virulent B. abortus

A A Oñate et al. Infect Immun. 1999 Feb.

Abstract

Vaccination of mice with Escherichia coli expressing Brucella Cu/Zn superoxide dismutase (SOD) [E. coli(pBSSOD)] induced a significant level of protection against virulent Brucella abortus challenge, although this level was not as high as the one reached with B. abortus vaccine strain RB51. In addition, vaccination with E. coli(pBSSOD) induced antibodies to Cu/Zn SOD and a strong proliferative response of splenocytes when stimulated in vitro with a thioredoxin-Cu/Zn SOD fusion protein.

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Figures

FIG. 1

FIG. 1

Western blot showing the expression of B. abortus Cu/Zn SOD protein by recombinant E. coli. The antigens separated by sodium dodecyl sulfate–15% polyacrylamide gel electrophoresis were transferred to a nitrocellulose membrane and reacted with different sera. The membranes were developed with appropriate secondary antibodies conjugated with horseradish peroxidase. Lanes 1 to 3 contain the antigens of B. abortus RB51, E. coli(pBSSOD), and E. coli(pBS), respectively. Panels A to C were reacted with hyperimmunized goat serum to sonicates of B. abortus RB51, goat antiserum to B. abortus Cu/Zn SOD protein, and sera from mice vaccinated with strain RB51, respectively. The arrow at the left of panel B indicates the Cu/Zn SOD band. Numbers at the left of panel A indicate the positions of molecular mass markers, in kilodaltons.

References

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