The interaction of epsin and Eps15 with the clathrin adaptor AP-2 is inhibited by mitotic phosphorylation and enhanced by stimulation-dependent dephosphorylation in nerve terminals - PubMed (original) (raw)
. 1999 Feb 5;274(6):3257-60.
doi: 10.1074/jbc.274.6.3257.
Affiliations
- PMID: 9920862
- DOI: 10.1074/jbc.274.6.3257
Free article
The interaction of epsin and Eps15 with the clathrin adaptor AP-2 is inhibited by mitotic phosphorylation and enhanced by stimulation-dependent dephosphorylation in nerve terminals
H Chen et al. J Biol Chem. 1999.
Free article
Abstract
Clathrin-mediated endocytosis was shown to be arrested in mitosis due to a block in the invagination of clathrin-coated pits. A Xenopus mitotic phosphoprotein, MP90, is very similar to an abundant mammalian nerve terminal protein, epsin, which binds the Eps15 homology (EH) domain of Eps15 and the alpha-adaptin subunit of the clathrin adaptor AP-2. We show here that both rat epsin and Eps15 are mitotic phosphoproteins and that their mitotic phosphorylation inhibits binding to the appendage domain of alpha-adaptin. Both epsin and Eps15, like other cytosolic components of the synaptic vesicle endocytic machinery, undergo constitutive phosphorylation and depolarization-dependent dephosphorylation in nerve terminals. Furthermore, their binding to AP-2 in brain extracts is enhanced by dephosphorylation. Epsin together with Eps15 was proposed to assist the clathrin coat in its dynamic rearrangements during the invagination/fission reactions. Their mitotic phosphorylation may be one of the mechanisms by which the invagination of clathrin-coated pits is blocked in mitosis and their stimulation-dependent dephosphorylation at synapses may contribute to the compensatory burst of endocytosis after a secretory stimulus.
Similar articles
- Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis.
Chen H, Fre S, Slepnev VI, Capua MR, Takei K, Butler MH, Di Fiore PP, De Camilli P. Chen H, et al. Nature. 1998 Aug 20;394(6695):793-7. doi: 10.1038/29555. Nature. 1998. PMID: 9723620 - The epsins define a family of proteins that interact with components of the clathrin coat and contain a new protein module.
Rosenthal JA, Chen H, Slepnev VI, Pellegrini L, Salcini AE, Di Fiore PP, De Camilli P. Rosenthal JA, et al. J Biol Chem. 1999 Nov 26;274(48):33959-65. doi: 10.1074/jbc.274.48.33959. J Biol Chem. 1999. PMID: 10567358 - Regulation of complex formation of POB1/epsin/adaptor protein complex 2 by mitotic phosphorylation.
Kariya K, Koyama S, Nakashima S, Oshiro T, Morinaka K, Kikuchi A. Kariya K, et al. J Biol Chem. 2000 Jun 16;275(24):18399-406. doi: 10.1074/jbc.M000521200. J Biol Chem. 2000. PMID: 10764745 - Epidermal growth factor pathway substrate 15, Eps15.
Salcini AE, Chen H, Iannolo G, De Camilli P, Di Fiore PP. Salcini AE, et al. Int J Biochem Cell Biol. 1999 Aug;31(8):805-9. doi: 10.1016/s1357-2725(99)00042-4. Int J Biochem Cell Biol. 1999. PMID: 10481267 Review. - Epsin: inducing membrane curvature.
Horvath CA, Vanden Broeck D, Boulet GA, Bogers J, De Wolf MJ. Horvath CA, et al. Int J Biochem Cell Biol. 2007;39(10):1765-70. doi: 10.1016/j.biocel.2006.12.004. Epub 2007 Jan 17. Int J Biochem Cell Biol. 2007. PMID: 17276129 Review.
Cited by
- Targeting Liver Epsins Ameliorates Dyslipidemia in Atherosclerosis.
Zhu B, Gupta K, Cui K, Wang B, Malovichko MV, Han X, Li K, Wu H, Arulsamy KS, Singh B, Gao J, Wong S, Cowan DB, Wang D, Biddinger S, Srivastava S, Shi J, Chen K, Chen H. Zhu B, et al. bioRxiv [Preprint]. 2024 Aug 27:2024.08.26.609742. doi: 10.1101/2024.08.26.609742. bioRxiv. 2024. PMID: 39253478 Free PMC article. Preprint. - Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis.
Ogunmowo TH, Jing H, Raychaudhuri S, Kusick GF, Imoto Y, Li S, Itoh K, Ma Y, Jafri H, Dalva MB, Chapman ER, Ha T, Watanabe S, Liu J. Ogunmowo TH, et al. Nat Commun. 2023 May 20;14(1):2888. doi: 10.1038/s41467-023-38595-2. Nat Commun. 2023. PMID: 37210439 Free PMC article. - Endocytic Adaptors in Cardiovascular Disease.
Cui K, Dong Y, Wang B, Cowan DB, Chan SL, Shyy J, Chen H. Cui K, et al. Front Cell Dev Biol. 2020 Dec 11;8:624159. doi: 10.3389/fcell.2020.624159. eCollection 2020. Front Cell Dev Biol. 2020. PMID: 33363178 Free PMC article. Review. - Epsin-mediated degradation of IP3R1 fuels atherosclerosis.
Dong Y, Lee Y, Cui K, He M, Wang B, Bhattacharjee S, Zhu B, Yago T, Zhang K, Deng L, Ouyang K, Wen A, Cowan DB, Song K, Yu L, Brophy ML, Liu X, Wylie-Sears J, Wu H, Wong S, Cui G, Kawashima Y, Matsumoto H, Kodera Y, Wojcikiewicz RJH, Srivastava S, Bischoff J, Wang DZ, Ley K, Chen H. Dong Y, et al. Nat Commun. 2020 Aug 7;11(1):3984. doi: 10.1038/s41467-020-17848-4. Nat Commun. 2020. PMID: 32770009 Free PMC article. - Regulation of the Total Cell Surface Area in Dividing Dictyostelium Cells.
Tanaka M, Fujimoto K, Yumura S. Tanaka M, et al. Front Cell Dev Biol. 2020 Apr 8;8:238. doi: 10.3389/fcell.2020.00238. eCollection 2020. Front Cell Dev Biol. 2020. PMID: 32322581 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials
Miscellaneous