Control of 3' splice site choice in vivo by ASF/SF2 and hnRNP A1 - PubMed (original) (raw)
Control of 3' splice site choice in vivo by ASF/SF2 and hnRNP A1
Y Bai et al. Nucleic Acids Res. 1999.
Abstract
The constitutive splicing factor ASF/SF2 has been shown to affect the choice between alternative splice sites by favoring the proximal as opposed to the distal choice. HnRNP A1 antagonizes ASF/SF2 by promoting the distal choice for competing 5' splice sites. We have tested the in vivo effects of these proteins on alternative 3' splice site choices. Cotransfection of a dihydrofolate reductase-calcitonin chimeric construct togetherwith a plasmid specifying the SR protein ASF/SF2 into cells of several mammalian lines increased use of a proximal 3' splice site, resulting in the inclusion of a terminal calcitonin exon. This stimulation of 3' proximal splicing was antagonized by cotransfection with an hnRNP A1 plasmid. This effect of hnRNP A1 in promoting distal splicing was also seen in an hnRNP A1-deficient MEL cell line. A similar effect of hnRNP A1 was demonstrated with mutant hamster adenine phosphoribosyltransferase (aprt) transcripts that are normally constitutively spliced, suggesting that hnRNP A1 may be a general inhibitor of proximal splicing. Intron size also influenced splice site choice in mutant aprt transcripts, with larger introns favoring proximal splicing. These results support the idea that the ratios of particular but general splicing factors and hnRNPs play a role in alternative splicing.
Similar articles
- Modulation of exon skipping and inclusion by heterogeneous nuclear ribonucleoprotein A1 and pre-mRNA splicing factor SF2/ASF.
Mayeda A, Helfman DM, Krainer AR. Mayeda A, et al. Mol Cell Biol. 1993 May;13(5):2993-3001. doi: 10.1128/mcb.13.5.2993-3001.1993. Mol Cell Biol. 1993. PMID: 8474457 Free PMC article. - Selection of alternative 5' splice sites: role of U1 snRNP and models for the antagonistic effects of SF2/ASF and hnRNP A1.
Eperon IC, Makarova OV, Mayeda A, Munroe SH, Cáceres JF, Hayward DG, Krainer AR. Eperon IC, et al. Mol Cell Biol. 2000 Nov;20(22):8303-18. doi: 10.1128/MCB.20.22.8303-8318.2000. Mol Cell Biol. 2000. PMID: 11046128 Free PMC article. - hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B.
Expert-Bezançon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. Expert-Bezançon A, et al. J Biol Chem. 2004 Sep 10;279(37):38249-59. doi: 10.1074/jbc.M405377200. Epub 2004 Jun 18. J Biol Chem. 2004. PMID: 15208309 - ASF/SF2: a splice site selector.
Lamond AI. Lamond AI. Trends Biochem Sci. 1991 Dec;16(12):452-3. doi: 10.1016/0968-0004(91)90176-v. Trends Biochem Sci. 1991. PMID: 1781021 Review. No abstract available. - hnRNP A1 in RNA metabolism regulation and as a potential therapeutic target.
Feng J, Zhou J, Lin Y, Huang W. Feng J, et al. Front Pharmacol. 2022 Oct 21;13:986409. doi: 10.3389/fphar.2022.986409. eCollection 2022. Front Pharmacol. 2022. PMID: 36339596 Free PMC article. Review.
Cited by
- Decoding complexity in biomolecular recognition of DNA i-motifs with microarrays.
Yazdani K, Seshadri S, Tillo D, Yang M, Sibley CD, Vinson C, Schneekloth JS Jr. Yazdani K, et al. Nucleic Acids Res. 2023 Dec 11;51(22):12020-12030. doi: 10.1093/nar/gkad981. Nucleic Acids Res. 2023. PMID: 37962331 Free PMC article. - Opposing roles of CLK SR kinases in controlling HIV-1 gene expression and latency.
Dahal S, Clayton K, Been T, Fernet-Brochu R, Ocando AV, Balachandran A, Poirier M, Maldonado RK, Shkreta L, Boligan KF, Guvenc F, Rahman F, Branch D, Bell B, Chabot B, Gray-Owen SD, Parent LJ, Cochrane A. Dahal S, et al. Retrovirology. 2022 Aug 19;19(1):18. doi: 10.1186/s12977-022-00605-4. Retrovirology. 2022. PMID: 35986377 Free PMC article. - Dido3-dependent SFPQ recruitment maintains efficiency in mammalian alternative splicing.
Mora Gallardo C, Sánchez de Diego A, Gutiérrez Hernández J, Talavera-Gutiérrez A, Fischer T, Martínez-A C, van Wely KHM. Mora Gallardo C, et al. Nucleic Acids Res. 2019 Jun 4;47(10):5381-5394. doi: 10.1093/nar/gkz235. Nucleic Acids Res. 2019. PMID: 30931476 Free PMC article. - SR proteins regulate V6 exon splicing of CD44 pre-mRNA.
Loh TJ, Moon H, Jang HN, Liu Y, Choi N, Shen S, Williams DR, Jung DW, Zheng X, Shen H. Loh TJ, et al. BMB Rep. 2016 Nov;49(11):612-616. doi: 10.5483/bmbrep.2016.49.11.118. BMB Rep. 2016. PMID: 27530682 Free PMC article. - The adipogenic transcriptional cofactor ZNF638 interacts with splicing regulators and influences alternative splicing.
Du C, Ma X, Meruvu S, Hugendubler L, Mueller E. Du C, et al. J Lipid Res. 2014 Sep;55(9):1886-96. doi: 10.1194/jlr.M047555. Epub 2014 Jul 14. J Lipid Res. 2014. PMID: 25024404 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials