Immunohistochemical and ultrastructural studies of basal cells, Clara cells and bronchiolar cuboidal cells in normal human airways - PubMed (original) (raw)
Immunohistochemical and ultrastructural studies of basal cells, Clara cells and bronchiolar cuboidal cells in normal human airways
M Nakajima et al. Pathol Int. 1998 Dec.
Abstract
Immunohistochemical studies were made of the distribution of various cytokeratins (CK), Clara cell secretory protein (CC10), surfactant protein A (SP-A) and type VII collagen in normal human airways. Electron microscopic studies were made to identify hemidesmosomes and anchoring fibrils on the basal surfaces of the epithelial cells. CK19 was detected in all epithelial cells, and CK17 in all basal cells. CK14 was coexpressed in a few basal cells, and this coexpression was decreased in the distal airways. Two types of basal cells were recognized. One type, found mainly in large airways, was characterized by abundant intermediate filaments and well-developed hemidesmosomes and anchoring fibrils. The second type contained few intermediate filaments and poorly developed hemidesmosomes and anchoring fibrils. Reactivity for type VII collagen was found along the basement membrane throughout the airways, but not in the alveoli. Clara cells were reactive for CC10 and CK17, but not for CK14 and SP-A. The bronchiolar cuboidal cells in the respiratory bronchioles were positive only for CK19. Surfactant protein A was present only in type II alveolar epithelial cells. Thus, two types of basal cells are present in airways, and the bronchiolar cuboidal cells appear distinct from these basal cells, Clara cells and type II alveolar epithelial cells.
Similar articles
- Clara cell differentiation in the mouse: ultrastructural morphology and cytochemistry for surfactant protein A and Clara cell 10 kD protein.
Ten Have-Opbroek AA, De Vries EC. Ten Have-Opbroek AA, et al. Microsc Res Tech. 1993 Dec 1;26(5):400-11. doi: 10.1002/jemt.1070260508. Microsc Res Tech. 1993. PMID: 8286786 - Phenotype of regenerative epithelium in idiopathic interstitial pneumonias.
Hinata N, Takemura T, Ikushima S, Yanagawa T, Ando T, Okada J, Oritsu M, Koike M. Hinata N, et al. J Med Dent Sci. 2003 Sep;50(3):213-24. J Med Dent Sci. 2003. PMID: 15074359 - Cell-specific expression of CCAAT/enhancer-binding protein delta (C/EBP delta) in epithelial lung cells.
Låg M, Skarpen E, van Rozendaal BA, Haagsman HP, Huitfeldt HS, Thrane EV, Schwarze PE. Låg M, et al. Exp Lung Res. 2000 Jul-Aug;26(5):383-99. doi: 10.1080/019021400408326. Exp Lung Res. 2000. PMID: 10914335 - Pulmonary epithelial stem cells.
Bishop AE. Bishop AE. Cell Prolif. 2004 Feb;37(1):89-96. doi: 10.1111/j.1365-2184.2004.00302.x. Cell Prolif. 2004. PMID: 14871239 Free PMC article. Review.
Cited by
- Lung Regeneration: Cells, Models, and Mechanisms.
Konkimalla A, Tata A, Tata PR. Konkimalla A, et al. Cold Spring Harb Perspect Biol. 2022 Oct 3;14(10):a040873. doi: 10.1101/cshperspect.a040873. Cold Spring Harb Perspect Biol. 2022. PMID: 34750172 Free PMC article. Review. - Bronchiolar Adenoma: Expansion of the Concept of Ciliated Muconodular Papillary Tumors With Proposal for Revised Terminology Based on Morphologic, Immunophenotypic, and Genomic Analysis of 25 Cases.
Chang JC, Montecalvo J, Borsu L, Lu S, Larsen BT, Wallace WD, Sae-Ow W, Mackinnon AC, Kim HR, Bowman A, Sauter JL, Arcila ME, Ladanyi M, Travis WD, Rekhtman N. Chang JC, et al. Am J Surg Pathol. 2018 Aug;42(8):1010-1026. doi: 10.1097/PAS.0000000000001086. Am J Surg Pathol. 2018. PMID: 29846186 Free PMC article. - Stem cells and lung regeneration.
Parekh KR, Nawroth J, Pai A, Busch SM, Senger CN, Ryan AL. Parekh KR, et al. Am J Physiol Cell Physiol. 2020 Oct 1;319(4):C675-C693. doi: 10.1152/ajpcell.00036.2020. Epub 2020 Aug 12. Am J Physiol Cell Physiol. 2020. PMID: 32783658 Free PMC article. Review. - Airway basal stem cells: a perspective on their roles in epithelial homeostasis and remodeling.
Rock JR, Randell SH, Hogan BL. Rock JR, et al. Dis Model Mech. 2010 Sep-Oct;3(9-10):545-56. doi: 10.1242/dmm.006031. Epub 2010 Aug 10. Dis Model Mech. 2010. PMID: 20699479 Free PMC article. Review. - Basal cells of differentiated bronchial epithelium are more susceptible to rhinovirus infection.
Jakiela B, Brockman-Schneider R, Amineva S, Lee WM, Gern JE. Jakiela B, et al. Am J Respir Cell Mol Biol. 2008 May;38(5):517-23. doi: 10.1165/rcmb.2007-0050OC. Epub 2007 Dec 6. Am J Respir Cell Mol Biol. 2008. PMID: 18063839 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous