Samad Jamali | Razi University of Kermanshah, Iran (original) (raw)

Papers by Samad Jamali

Research Square (Research Square), Apr 3, 2024

Forests are yeast biodiversity hotspots. In a taxonomic study of yeast isolates collected from th... more Forests are yeast biodiversity hotspots. In a taxonomic study of yeast isolates collected from the Persian oak (Quercus brantii Lind.) branch in the Zagros oak forests, Kermanshah province, Iran, one yeast isolate was found to represent a novel species. Morphological and physiological characteristics as well as sequence analysis of the D1/D2 region of the large subunit rRNA gene and the internal transcribed spacer region showed that the novel species belonged to the genus Fonsecazyma (Tremellales, Bulleraceae). The name Fonsecazyma quercina sp. nov. is proposed for this new species. Phylogenetic analysis based on D1/D2 and ITS sequences showed that strain IRAN 18507F belongs to the genus Fonsecazyma and has the highest sequence similarity with Fonsecazyma sp. KT301 (97.58%). This isolate consisted of spherical single cells and could grow up to 1 M NaCl. It grew at pH 4, 8, and 10, but not at the acidic pH of 2. The isolate is psychrotolerant, growing well between 6°C and 25°C, but not at temperatures outside this range. The isolate e ciently utilized and assimilated glucose and trehalose as carbon sources; it could also utilize sucrose, mannose, maltose, and fructose, but weakly utilized cellobiose, sorbitol, and rhamnose. This isolate could not utilize galactose, sorbose, lactose, melibiose, arabinose, ribose, or glycine as sole carbon sources. The holotype of Fonsecazyma quercina sp. nov. is IRAN 18507F (MycoBank number MB852634).

Indian phytopathology/Indian Phytopathology, Jun 24, 2024

Iran Agricultural Research, 2014

ABSTRACT- Desert truffles are hypogeous ascomyceteous ectomycorrhizal fungi, occurring in arid an... more ABSTRACT- Desert truffles are hypogeous ascomyceteous ectomycorrhizal fungi, occurring in arid and semi-arid ecosystems. A PCR-based method was developed for the identification of 3 major desert truffles of Iran: Terfezia claveryi, Tirmania pinoyi and Tirmania nivea based on internal transcribed spacers of rDNA. Two specific PCR primers were designed for T. claveryi, 4 for T. pinoyi, and 2 for T. nivea. All primers were specific to their homologous species and neither set amplified purified DNA from other truffle species or soil-borne micromycetes. In conventional PCR, the limits of detection were 10-500 pg DNA. ITS-Tfc1 primers detected the species in host tissues using a nested-PCR method by ITS1 and ITS4 universal primers as external set.

During 2009–10, the presence of ascomycetous species in soil was studied in Fars province. Isolat... more During 2009–10, the presence of ascomycetous species in soil was studied in Fars province. Isolates were recovered from soil using modified Czapek (Raper et al. 1973), malt extract agar (MEA) and potato dextrose agar (PDA). Teleomorphic characteristics such as morphology of ascocarps, asci, ascospores and anamorphic characteristics such as conidiophores, phialides, conidia and chlamydospores were investigated. Based on the above criteria two species were identified: Emericella echinulata and E. nidulans from which the former species is a new record for Iran.

Iran Agricultural Research, Sep 21, 2014

Desert truffles are hypogeous ascomyceteous ectomycorrhizal fungi, occurring in arid and semi-ari... more Desert truffles are hypogeous ascomyceteous ectomycorrhizal fungi, occurring in arid and semi-arid ecosystems. A PCR-based method was developed for the identification of 3 major desert truffles of Iran: Terfezia claveryi, Tirmania pinoyi and Tirmania nivea based on internal transcribed spacers of rDNA. Two specific PCR primers were designed for T. claveryi, 4 for T. pinoyi, and 2 for T. nivea. All primers were specific to their homologous species and neither set amplified purified DNA from other truffle species or soilborne micromycetes. In conventional PCR, the limits of detection were 10-500 pg DNA. ITS-Tfc1 primers detected the species in host tissues using a nested-PCR method by ITS1 and ITS4 universal primers as external set.

Iranian Journal of Plant Pathology, 2012

Rostaniha, Feb 20, 2011

The ITS region including the 5.8S gene of rDNA of three desert truffle species were amplified usi... more The ITS region including the 5.8S gene of rDNA of three desert truffle species were amplified using ITS4 and ITS1 primers. The ITS sequences were compared to those of other related authentic sequences obtained from GenBank. Among 12 specimens studied, seven isolates corresponded to Terfezia claveryi reported by other authors. Iranian T. claveryi specimens had an average similarity of 99.4% (range 98.7-100%) among themselves, while all T. claveryi sequences analyzed had an average of 95.2% (range 87.2-100%) similarity. Four specimens corresponded to Trimania pinoyi, being a sister taxon to Tirmania nivea, of which only one specimen could be studied. Iranian T. pinoyi specimens had an average of 99.9% similarity (range 99.8-100%) among themselves, and 97.2% (range 93.1-100%) between all T. pinoyi sequences compared. All T. nivea sequences, including Iranian specimen, had an average of 96.6% similarity (range 92.2-99.8%). T. claveryi and T. pinoyi are widespread in the country and are mostly associated with Helianthemum salicifolium and Carex sp. Iranian truffles flora resembles those of Mediterranean region and South coast of the Persian Gulf.

Research Square (Research Square), Apr 27, 2022

Tru e farming does not exist in Iran, and no formal studies have been conducted on its culture. F... more Tru e farming does not exist in Iran, and no formal studies have been conducted on its culture. For commercial production of Tirmania pinoyi, it is necessary to accurate detection of it in the roots of the host plants. In this study, for the rst time, mycorrhizal association of T. pinoyi with several perennial Cistaceae including Helianthemum lippii, H. almeriense, Cistus albidus, and Cistua ladanifer were investigated. In all plant species inoculated with Tirmania pinoyi, an ectendomycorrhizal association with varying degrees of sheath development was observed. Our results shows that H. lippii is suitable plant host for Tirmania pinoyi, and mycorrhization rate and the relative mycorrhizal dependency (RMD) in this native plant was about 90% and 57.85%, respectively, and higher than the other three plant species. The effect of the mycorrhizal fungus on host growth (root weight, root height, shoot weight, shoot height, plant height, and plant weight) was statistically signi cant compared to non-inoculated plants. To detect the roots colonized by T. pinoyi, a PCR based diagnostic method was developed with the species-speci c primer pairs FTiPi and RTiPi designed from the sequence of the internal transcribed space regions (ITS1 and ITS2). The speci city of the primers was veri ed by PCR analysis of DNA from T. pinoyi specimens and other desert tru es. The method detected T. pinoyi in arti cialy infected root plants, and no cross-reactions were observed with any other tested desert tru es. This species-speci c PCR method is suitable for quick, simple, and reliable detection of T. pinoyi mycorrhizas.

Rostaniha, Sep 1, 2020

An investigation was carried out on the occurrence and distribution of entomopathogenic fungi in ... more An investigation was carried out on the occurrence and distribution of entomopathogenic fungi in 41 soil samples collected from cultivated and natural regions in Kermanshah province (West of Iran) from July 2017 to April 2018. Among 41 soil samples, 114 fungal isolates were recovered with 39 from forests, 38 from fields, 22 from rangelands and 15 from garden soil. Based on the morphological characters and phylogeny of DNA sequence data for the internal transcribed spacer (ITS) rDNA and comparing the sequences with that available in NCBI database, the entomopathogenic fungi recovered were identified as:

DOAJ (DOAJ: Directory of Open Access Journals), Dec 1, 2018

Neocosmospora E.F. Smith is a filamentous ascomycete fungal genus belong to Hypocreales order and... more Neocosmospora E.F. Smith is a filamentous ascomycete fungal genus belong to Hypocreales order and contains several species mainly pathogenic for plants (Cannon & Hawksworth 1982). Species of Neocosmospora are known to live in the soil of

Journal of Crop Protection, Feb 16, 2013

A specimen of an ascomycetous fungus collected during a field trip was identified as Gymnoascus B... more A specimen of an ascomycetous fungus collected during a field trip was identified as Gymnoascus Baran. Soil samples collected from 0-20 cm depth were studied for isolation of fungi using a soil dilution plate method. Based on the morphological and molecular characters the specimens were identified as Gymnoascus reesii. Genomic DNA was extracted and a nuclear rDNA region, containing the internal transcribed spacers 1, 2 and 5.8S gene of rDNA (ITS) were amplified and PCR products were sequenced. Amplicon was purified, sequenced and submitted to the GenBank (Acc. No. JQ387570-71). The resulting sequence (600 bp) was submitted to a BLAST search to find most similar sequences in GenBank. The search results showed highest similarity of Iranian isolates to other isolates of G. reesii from GenBank. In the light of literature on ascomycetous fungi, Gymnoascus Baran is a new record for Iran mycoflora. The specimens are kept in fungal collection of the

DOAJ (DOAJ: Directory of Open Access Journals), Feb 1, 2012

ɷ 12) 2 :(194-193) 1390 (-ɺɷɳ ʇ First report of Emericella echinulata as a teleomorph of Aspergil... more ɷ 12) 2 :(194-193) 1390 (-ɺɷɳ ʇ First report of Emericella echinulata as a teleomorph of Aspergillus from Iran ɸɳ ɺɷɳ Emericella echinulata

Rostaniha, Jun 30, 2020

An investigation was carried out on the occurrence of thermotolerant and thermophilic fungi in 11... more An investigation was carried out on the occurrence of thermotolerant and thermophilic fungi in 11 soil samples collected from cultivated and natural regions in Kermanshah province (Mahidasht, Harsin, Kerend, Eslamabad-e Gharb, Qasr-e Shirin, Sarpol-e Zahab, Javanrood, Gilan-e Gharb), municipal waste compost and mushroom compost, 2017-19. Fungal isolates were recovered using the soil dilution plate method on potato dextrose agar at 45 and 50 °C. Totally, 24 isolates were obtained that were primarily identified using morphological characters and referring to valid taxonomic keys. DNA extraction was carried out using a Genomic DNA Purification kit. The ITS region (ITS1-5.8S-ITS2) of the ribosomal DNA was amplified using ITS1 and ITS4 primers. Fragments about 500-700 bp were amplified after sequencing deposited in GenBank. Based on morphological characters and sequence data of the ITS rDNA, these fungi were identified as: Aspergillus fumigatus, A. nidulans, A. niger, A. terrus, Melanocarpus albomyces*, Malbranchea cinnamomea*, Thermomyces dupontii*, Th. lanuginosus*, and Thielavia arenaria*. Asterisks indicate species that are new records for the mycobiota of Iran. The abundance of thermophilic fungi in municipal waste compost was higher than soil, and Aspergillus species were the most abundant fungi identified in this study.

Rostaniha, Feb 20, 2012

First report of Chromelosporium fulvum from Iran ɸɳ ɺɷɳ Chromelosporium fulvum

DOAJ (DOAJ: Directory of Open Access Journals), Feb 1, 2017

Rhizopogonیک قارچ زیرزمینی است که به صورت قارچ-ریشه خارجی در همزیستی با اغلب... more Rhizopogonیک قارچ زیرزمینی است که به صورت قارچ-ریشه خارجی در همزیستی با اغلب اعضای تیره کاج‌ (Pinaceae) بوده و پراکنش جهانی آن همسو با جنگل‌های کاج است. در ایران، مطالعات کمی در خصوص Rhizopogon صورت گرفته و اطلاعات اندکی در مورد این قارچ در دسترس است. در مطالعه حاضر، هفت نمونهRhizopogon که همراه با ریشه گونه گیاهی Pinus eldarica بودند، براساس خصوصیات مورفولوژیکی و مولکولی مورد بررسی قرار گرفتند. بر این اساس، تمام نمونه‌های دنبل دروغینRhizopogon همراه با ریشه این گونه گیاهی متعلق به جنسRhizopogon بودند. توالی دی.ان.ای. نمونه‌های Rhizopogon 100 درصد همولوژی با نمونه‌های معتبر ثبت شده در بانک ژن داشتند. درخت فیلوژنتیکی ترسیم شده براساس توالی‌های جداکننده نسخه‌برداری شده داخلی (آی.تی.اس.)، نشان داد که تمام نمونه‌های تحت بررسی با نمونه‌های معتبر مربوط به گونه Rhizopogon roseolus در یک شاخه با ضریب اطمینان بالا قرار گرفتند. این نخستین گزارش از وجود این گونه و میزبان آن در ایران است.

Journal of Plant Pathology, Dec 6, 2022

x trees under controlled conditions. The results of the pathogenicity test demonstrated that all ... more x trees under controlled conditions. The results of the pathogenicity test demonstrated that all the isolates of P. formosus were pathogenic to inoculated branches and plants. Re-isolation of pathogen from all inoculated plants after observation of symptoms were performed, thus completing Koch's postulates. P. formosus has been reported to infect a broad range of hosts (Sabernasab et al. 2019; Rostami and Jamali 2022). This study represents the first report of this species on A. foetida worldwide. Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Brazilian Journal of Microbiology, Apr 19, 2021

Four hundred endophytic fungi isolates with different colony morphologies were isolated from root... more Four hundred endophytic fungi isolates with different colony morphologies were isolated from roots of Hordeum vulgare L. collected from un-engineered landfills (the measured cadmium was 0.9 mg kg−1) of Kermanshah province in West Iran. Based on morphology and phylogeny of DNA sequence data for the internal transcribed spacer (ITS) rDNA and comparing the sequences with that available in NCBI database, 11 isolates are identified as dark septate endophytes (DSE) including Alternaria alternata, Microdochium bolleyi, Bipolaris zeicola, Alternaria sp., and Pleosporales sp., and the other nine are not dark septate endophytes (non-DSE) including Fusarium redolens, Fusarium tricinctum, Fusarium monliforme, Clonostachys rosea, and Epicoccum nigrum. Tolerance of DSE and non-DSE strains for Cd were investigated in potato dextrose agar medium. Minimum inhibitory concentrations (MIC) of Cd from nitrate salt source (Cd (NO3)2) and EC50 were determined. The means of MIC and EC50 values for DSE fungi species were 1254.5 and 209.74 mg/kg, compared to 800 and 150.3 mg/kg for non-DSEs. Among the endophytic fungi isolated, Alternaria sp. (TBR5) and Bipolaris zeicola (Tw26) showed the highest tolerance to Cd with a MIC value of 2000 mg/L and 1800 mg/L, respectively. Barley plants were inoculated with TBR5 and Tw26 in Cd-added sands (0, 10, 30, 60 mg Cd/kg sand). In terms of Cd accumulation, our results showed that TBR5 and Tw26 inoculation increased the amount of Cd in the barley roots. TBR5 and Tw26 significantly improved (p < 0.05) plant growth in the presence of Cd by enhancing plant growth attributes such as chlorophyll content, root weight, plant length, fresh weight, and dry weight of plants. This is the first study on the abundance and identification of endophytic root fungi of barley in a cadmium-contaminated soil in Iran. The results of this study showed that DSE and non-DSE have the potential to improve the efficiency of phytoremediation.

Indian phytopathology, Jun 29, 2021