vishal jain | Rajiv Gandhi University of Health Sciences (original) (raw)

Papers by vishal jain

Research paper thumbnail of Isolation and standardization of various phytochemical constituents from methanolic extracts of fruit rinds of Punica granatum

Chinese Journal of Natural Medicines, 2011

The present study was undertaken to isolate and standardize the various active phytochemical cons... more The present study was undertaken to isolate and standardize the various active phytochemical constituents present in the fruit rinds of Punica granatum. METHODS: Fruit rinds of Punica granatum were dried and extracted with methanol in a static extractor; the percentage yield of the methanolic extract (MEPG) was found to be 26%; the methanolic extract was partitioned using n-butanol, ethyl acetate and water; the percentage yield of the fractions were found to be 17.16%, 26.88% and 47.72% respectively. HPLC was carried out for all the fractions and the markers corresponding to the major peaks in HPLC were isolated using column chromatography. The isolated markers were characterized using UV, IR, Mass and NMR spectroscopy. RESULTS & CONCLUSION: Totally four compounds were isolated and identified as punicalin, punicalagin, gallic acid and ellagic acid based on UV, IR, Mass and NMR data, they were standardized by HPLC.

Research paper thumbnail of Isolation of Antidiabetic Principle from Fruit Rinds ofPunica granatum

Evidence-Based Complementary and Alternative Medicine, 2012

Present study was aimed to isolate and evaluate the antidiabetic activity of phytoconstituents fr... more Present study was aimed to isolate and evaluate the antidiabetic activity of phytoconstituents from fruit rinds ofPunica granatum. With the above objectives Valoneic acid dilactone (VAD) was isolated from methanolic fruit rind extracts ofPunica granatum(MEPG) and confirmed by1H-NMR,13C-NMR, and mass spectral data. Antidiabetic activity was evaluated by Aldose reductase,α-amylase and PTP1B inhibition assays inin vitroand Alloxan-induced diabetes in rats was used as anin vivomodel. In bioactivity studies, MEPG and VAD have showed potent antidiabetic activity inα-amylase, aldose reductase, and PTP1B inhibition assays with IC50values of 1.02, 2.050, 26.25 μg/mL and 0.284, 0.788, 12.41 μg/mL, respectively. Furthermore, in alloxan-induced diabetes model MEPG (200 and 400 mg/kg, p.o.) and VAD (10, 25, and 50 mg/kg, p.o.) have showed significant and dose dependent antidiabetic activity by maintaining the blood glucose levels within the normal limits. Inline with the biochemical findings his...

Research paper thumbnail of Isolation and standardization of various phytochemical constituents from methanolic extracts of fruit rinds of Punica granatum

Chinese Journal of Natural Medicines, 2011

AIM: The present study was undertaken to isolate and standardize the various active phytochemical... more AIM: The present study was undertaken to isolate and standardize the various active phytochemical constituents present in the fruit rinds of Punica granatum. METHODS: Fruit rinds of Punica granatum were dried and extracted with methanol in a static extractor; the percentage yield of the methanolic extract (MEPG) was found to be 26%; the methanolic extract was partitioned using n-butanol, ethyl acetate and water; the percentage yield of the fractions were found to be 17.16%, 26.88% and 47.72% respectively. HPLC was carried out for all the fractions and the markers corresponding to the major peaks in HPLC were isolated using column chromatography. The isolated markers were characterized using UV, IR, Mass and NMR spectroscopy. RESULTS & CONCLUSION: Totally four compounds were isolated and identified as punicalin, punicalagin, gallic acid and ellagic acid based on UV, IR, Mass and NMR data, they were standardized by HPLC. [KEY WORDS] Punica granatum; Punicalin; Punicalagin; Gallic acid and ellagic acid [CLC Number] R284 [Document code] A [Article ID] 1672-3651(2011)06-0414-07 Vishal Jain, et al.

Research paper thumbnail of Chinese Journal of Natural Medicines Isolation and Standardization of Various Phytochemical Constituents from Methanolic Extracts of Fruit Rinds of Punica granatum

AIM: The present study was undertaken to isolate and standardize the various active phytochemical... more AIM: The present study was undertaken to isolate and standardize the various active phytochemical constituents present in the fruit rinds of Punica granatum. METHODS: Fruit rinds of Punica granatum were dried and extracted with methanol in a static extractor; the percentage yield of the methanolic extract (MEPG) was found to be 26%; the methanolic extract was partitioned using n-butanol, ethyl acetate and water; the percentage yield of the fractions were found to be 17.16%, 26.88% and 47.72% respectively. HPLC was carried out for all the fractions and the markers corresponding to the major peaks in HPLC were isolated using column chromatography. The isolated markers were characterized using UV, IR, Mass and NMR spectroscopy. RESULTS & CONCLUSION: Totally four compounds were isolated and identified as punicalin, punicalagin, gallic acid and ellagic acid based on UV, IR, Mass and NMR data, they were standardized by HPLC. [KEY WORDS] Punica granatum; Punicalin; Punicalagin; Gallic acid and ellagic acid [CLC Number] R284 [Document code] A [Article ID] 1672-3651(2011)06-0414-07 Vishal Jain, et al.

Research paper thumbnail of Isolation of Antidiabetic Principle from Fruit Rinds of Punica granatum

Present study was aimed to isolate and evaluate the antidiabetic activity of phytoconstituents fr... more Present study was aimed to isolate and evaluate the antidiabetic activity of phytoconstituents from fruit rinds of Punica granatum. With the above objectives Valoneic acid dilactone (VAD) was isolated from methanolic fruit rind extracts of Punica granatum (MEPG) and confirmed by 1 H-NMR, 13 C-NMR, and mass spectral data. Antidiabetic activity was evaluated by Aldose reductase, α-amylase and PTP1B inhibition assays in in vitro and Alloxan-induced diabetes in rats was used as an in vivo model. In bioactivity studies, MEPG and VAD have showed potent antidiabetic activity in α-amylase, aldose reductase, and PTP1B inhibition assays with IC 50 values of 1.02, 2.050, 26.25 μg/mL and 0.284, 0.788, 12.41 μg/mL, respectively. Furthermore, in alloxan-induced diabetes model MEPG (200 and 400 mg/kg, p.o.) and VAD (10, 25, and 50 mg/kg, p.o.) have showed significant and dose dependent antidiabetic activity by maintaining the blood glucose levels within the normal limits. Inline with the biochemical findings histopathology of MEPG (200 and 400 mg/kg, p.o.), VAD (10, and 50 mg/kg, p.o.), and glibenclamide (10 mg/kg, p.o.) treated animals showed significant protection against alloxan-induced pancreatic tissue damage. These findings suggest that MEPG and VAD possess significant antidiabetic activity in both in vitro and in vivo models.

Research paper thumbnail of Isolation and standardization of various phytochemical constituents from methanolic extracts of fruit rinds of Punica granatum

Chinese Journal of Natural Medicines, 2011

The present study was undertaken to isolate and standardize the various active phytochemical cons... more The present study was undertaken to isolate and standardize the various active phytochemical constituents present in the fruit rinds of Punica granatum. METHODS: Fruit rinds of Punica granatum were dried and extracted with methanol in a static extractor; the percentage yield of the methanolic extract (MEPG) was found to be 26%; the methanolic extract was partitioned using n-butanol, ethyl acetate and water; the percentage yield of the fractions were found to be 17.16%, 26.88% and 47.72% respectively. HPLC was carried out for all the fractions and the markers corresponding to the major peaks in HPLC were isolated using column chromatography. The isolated markers were characterized using UV, IR, Mass and NMR spectroscopy. RESULTS & CONCLUSION: Totally four compounds were isolated and identified as punicalin, punicalagin, gallic acid and ellagic acid based on UV, IR, Mass and NMR data, they were standardized by HPLC.

Research paper thumbnail of Isolation of Antidiabetic Principle from Fruit Rinds ofPunica granatum

Evidence-Based Complementary and Alternative Medicine, 2012

Present study was aimed to isolate and evaluate the antidiabetic activity of phytoconstituents fr... more Present study was aimed to isolate and evaluate the antidiabetic activity of phytoconstituents from fruit rinds ofPunica granatum. With the above objectives Valoneic acid dilactone (VAD) was isolated from methanolic fruit rind extracts ofPunica granatum(MEPG) and confirmed by1H-NMR,13C-NMR, and mass spectral data. Antidiabetic activity was evaluated by Aldose reductase,α-amylase and PTP1B inhibition assays inin vitroand Alloxan-induced diabetes in rats was used as anin vivomodel. In bioactivity studies, MEPG and VAD have showed potent antidiabetic activity inα-amylase, aldose reductase, and PTP1B inhibition assays with IC50values of 1.02, 2.050, 26.25 μg/mL and 0.284, 0.788, 12.41 μg/mL, respectively. Furthermore, in alloxan-induced diabetes model MEPG (200 and 400 mg/kg, p.o.) and VAD (10, 25, and 50 mg/kg, p.o.) have showed significant and dose dependent antidiabetic activity by maintaining the blood glucose levels within the normal limits. Inline with the biochemical findings his...

Research paper thumbnail of Isolation and standardization of various phytochemical constituents from methanolic extracts of fruit rinds of Punica granatum

Chinese Journal of Natural Medicines, 2011

AIM: The present study was undertaken to isolate and standardize the various active phytochemical... more AIM: The present study was undertaken to isolate and standardize the various active phytochemical constituents present in the fruit rinds of Punica granatum. METHODS: Fruit rinds of Punica granatum were dried and extracted with methanol in a static extractor; the percentage yield of the methanolic extract (MEPG) was found to be 26%; the methanolic extract was partitioned using n-butanol, ethyl acetate and water; the percentage yield of the fractions were found to be 17.16%, 26.88% and 47.72% respectively. HPLC was carried out for all the fractions and the markers corresponding to the major peaks in HPLC were isolated using column chromatography. The isolated markers were characterized using UV, IR, Mass and NMR spectroscopy. RESULTS & CONCLUSION: Totally four compounds were isolated and identified as punicalin, punicalagin, gallic acid and ellagic acid based on UV, IR, Mass and NMR data, they were standardized by HPLC. [KEY WORDS] Punica granatum; Punicalin; Punicalagin; Gallic acid and ellagic acid [CLC Number] R284 [Document code] A [Article ID] 1672-3651(2011)06-0414-07 Vishal Jain, et al.

Research paper thumbnail of Chinese Journal of Natural Medicines Isolation and Standardization of Various Phytochemical Constituents from Methanolic Extracts of Fruit Rinds of Punica granatum

AIM: The present study was undertaken to isolate and standardize the various active phytochemical... more AIM: The present study was undertaken to isolate and standardize the various active phytochemical constituents present in the fruit rinds of Punica granatum. METHODS: Fruit rinds of Punica granatum were dried and extracted with methanol in a static extractor; the percentage yield of the methanolic extract (MEPG) was found to be 26%; the methanolic extract was partitioned using n-butanol, ethyl acetate and water; the percentage yield of the fractions were found to be 17.16%, 26.88% and 47.72% respectively. HPLC was carried out for all the fractions and the markers corresponding to the major peaks in HPLC were isolated using column chromatography. The isolated markers were characterized using UV, IR, Mass and NMR spectroscopy. RESULTS & CONCLUSION: Totally four compounds were isolated and identified as punicalin, punicalagin, gallic acid and ellagic acid based on UV, IR, Mass and NMR data, they were standardized by HPLC. [KEY WORDS] Punica granatum; Punicalin; Punicalagin; Gallic acid and ellagic acid [CLC Number] R284 [Document code] A [Article ID] 1672-3651(2011)06-0414-07 Vishal Jain, et al.

Research paper thumbnail of Isolation of Antidiabetic Principle from Fruit Rinds of Punica granatum

Present study was aimed to isolate and evaluate the antidiabetic activity of phytoconstituents fr... more Present study was aimed to isolate and evaluate the antidiabetic activity of phytoconstituents from fruit rinds of Punica granatum. With the above objectives Valoneic acid dilactone (VAD) was isolated from methanolic fruit rind extracts of Punica granatum (MEPG) and confirmed by 1 H-NMR, 13 C-NMR, and mass spectral data. Antidiabetic activity was evaluated by Aldose reductase, α-amylase and PTP1B inhibition assays in in vitro and Alloxan-induced diabetes in rats was used as an in vivo model. In bioactivity studies, MEPG and VAD have showed potent antidiabetic activity in α-amylase, aldose reductase, and PTP1B inhibition assays with IC 50 values of 1.02, 2.050, 26.25 μg/mL and 0.284, 0.788, 12.41 μg/mL, respectively. Furthermore, in alloxan-induced diabetes model MEPG (200 and 400 mg/kg, p.o.) and VAD (10, 25, and 50 mg/kg, p.o.) have showed significant and dose dependent antidiabetic activity by maintaining the blood glucose levels within the normal limits. Inline with the biochemical findings histopathology of MEPG (200 and 400 mg/kg, p.o.), VAD (10, and 50 mg/kg, p.o.), and glibenclamide (10 mg/kg, p.o.) treated animals showed significant protection against alloxan-induced pancreatic tissue damage. These findings suggest that MEPG and VAD possess significant antidiabetic activity in both in vitro and in vivo models.