Cindy O'Malley | RMIT University (original) (raw)
Papers by Cindy O'Malley
Journal of Science Education and Technology, Jan 20, 2016
A common trend in higher education is the "flipped" classroom, which facilitates active learning ... more A common trend in higher education is the "flipped" classroom, which facilitates active learning during class. The flipped approach to teaching was instituted in a haematology 'major' class and the students' attitudes and preferences for the teaching materials were surveyed. The curriculum design was explicit and involved four major components 1) the preparation of the students; 2) the weekly pre-class work; 3) the in-class active learning strategies and 4) closing the learning loop using formative quizzes. Each of these components is discussed in detail and was informed by sound pedagogical strategies. Several different sources of information and several freely available software tools to engage the students are discussed. Two iterations are reported here, with improved pass rate for the final examination from 47-48% in the traditional class to 56-65% in the flipped classroom approach. The majority of students (93% and 89%) came to the class prepared, after viewing the screencasts and engaged fully with the activities within the face-to-face time. The students perceived that solving case studies (93%) was the most beneficial activity for their learning and this was closely followed by the production of essay plans (71%). The majority of students recommended that this approach be repeated the following year (69% and 75%).
Thrombosis Research, Apr 1, 2019
BAK BCL 2 homologous antagonist killer BCL B-cell lymphoma BAX BCL 2 associated X protein BM Bone... more BAK BCL 2 homologous antagonist killer BCL B-cell lymphoma BAX BCL 2 associated X protein BM Bone marrow BSS Bernard-Soulier Syndrome cAMP Cyclic adenosine monophosphate CEACAM Carcinoembryonic antigen-releated cell adhesion molecule Ceacam1 Murine ceacam1 gene Ceacam2 Murine ceacam2 gene CRD-like Carbohydrate-like recognition domain CRPs Collagen-related peptide CVD Cardiovascular disease CLEC-2 C-type lectin-like receptor 2 CIB1 Calcium and integrin binding protein 1
Blood, Oct 9, 2014
• CEACAM2 is a novel platelet immunoreceptor. • CEACAM2 negatively regulates platelet-collagen in... more • CEACAM2 is a novel platelet immunoreceptor. • CEACAM2 negatively regulates platelet-collagen interactions and thrombus growth and stability in vitro, in vivo and CLEC-2 pathways. Carcinoembryonic antigen-related cell adhesion molecule-2 (CEACAM2) is a cell-surface glycoprotein expressed on blood, epithelial, and vascular cells. CEACAM2 possesses adhesive and signaling properties mediated by immunoreceptor tyrosine-based inhibitory motifs. In this study, we demonstrate that CEACAM2 is expressed on the surface and in intracellular pools of platelets. Functional studies of platelets from Ceacam2 2/2deficient mice (Cc2 2/2) revealed that CEACAM2 serves to negatively regulate collagen glycoprotein VI (platelet) (GPVI)-FcRg-chain and the C-type lectinlike receptor 2 (CLEC-2) signaling. Cc2 2/2 platelets displayed enhanced GPVI and CLEC-2-selective ligands, collagen-related peptide (CRP), collagen, and rhodocytin (Rhod)-mediated platelet aggregation. They also exhibited increased adhesion on type I collagen, and hyperresponsive CRP and CLEC-2-induced a and dense granule release compared with wild-type platelets. Furthermore, using intravital microscopy to ferric chloride (FeCl 3)-injured mesenteric arterioles and laser-induced injury of cremaster muscle arterioles, we herein show that thrombi formed in Cc2 2/2 mice were larger and more stable than wild-type controls in vivo. Thus, CEACAM2 is a novel platelet immunoreceptor that acts as a negative regulator of platelet GPVI-collagen interactions and of ITAM receptor CLEC-2 pathways.
Platelets, May 9, 2016
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is an Ig-ITIM superfamily mem... more Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is an Ig-ITIM superfamily member that regulates integrin αIIbβ3 function. We hypothesized that its twin protein, CEACAM2, exerts a similar physiologic role in murine platelets. CEACAM2-deficient mice (Cc2(-/-)) displayed prolonged tail bleeding times and increased volume of blood loss. Cc2(-/-) platelets have moderate integrin αIIbβ3-mediated functional defects with impaired kinetics of platelet spreading on fibrinogen and type I collagen and delayed kinetics in the retraction of fibrin clots in vitro. This functional integrin αIIbβ3 defect could not be attributed to altered integrin αIIbβ3 expression. Cc2(-/-) platelets displayed normal &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;inside-out&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; signaling properties as demonstrated by normal agonist-induced binding of soluble fluorescein isothiocyanate (FITC)-fibrinogen and JON/A antibody binding. This data provides direct evidence that disruption of CEACAM2 induces a moderate integrin αIIbβ3-mediated platelet function defect, and that CEACAM2 is essential to maintain a normal integrin αIIbβ3-mediated platelet function.
International Journal of Hygiene and Environmental Health
International Journal of Osteopathic Medicine
Blood, 1996
This report describes the effect of pegylated recombinant human megakaryocyte growth and developm... more This report describes the effect of pegylated recombinant human megakaryocyte growth and development factor (PEG-rHuMGDF) on platelet production and platelet function in humans. Subjects with advanced solid tumors received PEG-rHuMGDF daily for up to 10 days. There was no increase in circulating platelet count at doses of 0.03 or 0.1 microgram/kg/d by day 12 of study. At doses of 0.3 and 1.0 microgram/kg/d there was a threefold median increase (maximum 10-fold) in platelet count by day 16. The platelets produced in vivo in response to PEG-rHuMGDF showed unchanged aggregation and adenosine triphosphate (ATP)-release responses in in vitro assays. Tests included aggregation and release of ATP in response to adenosine diphosphate (ADP) (10, 5, 2.5, and 1.25 mumol/L), collagen (2 micrograms/mL), thrombin-receptor agonist peptide (TRAP, 10 mumol/L) and ristocetin (1.5 mg/mL). Administration of aspirin to an individual with platelet count of 1,771 x 10(3)/L resulted in the typical aspirin-...
Clinica Chimica Acta, 2019
Background-aim Biochemically Vitamin B12 deficiency causes decreased methionine synthase and L-Me... more Background-aim Biochemically Vitamin B12 deficiency causes decreased methionine synthase and L-Methylmalonyl-CoA Mutase activity, which results in accumulation of Homocysteine (HCY), Methylmalonic acid (MMA) and propionyl-l-carnitine (C3). Infant vitamin B12 deficiency is usually caused by maternal deficiency due to vegetarian diet or pernicious anemia (intrinsic factor antibodies (FI) and/or parietal cell antibodies (anti-CP)).The early recognition and treatment in infant deficiency can prevent potentially serious and irreversible neurologic damage. The maternal vitamin B12 deficiency can be detected through the expanded newborn screening by tandem mass spectrometry (MS/MS) with the high levels of C3, C3/C2 (propionyl-lcarnitine /acetyl-l-carnitine) ratio in the dried blood spot sample (DBS) of newborns. The aim of this study is to evaluate the prevalence of maternal cobalamine deficiency detected through expanded newborn screening.
Hematology & Transfusion International Journal, 2017
Thrombotic disorders are treated mostly with Heparin and Warfarin in cases requiring anticoagulat... more Thrombotic disorders are treated mostly with Heparin and Warfarin in cases requiring anticoagulation drugs. Due to limitations to these drugs including risk of bleeding and need for regular laboratory monitoring several Direct oral anticoagulants (DOACs) have been developed and shown to have more advantages than Warfarin. Nevertheless, these drugs are limited to use in some circumstances. As other anticoagulants, these drugs need to be managed and controlled to cope their over activities. Subsequently, many antidotes have been developed to reverse these DOACs. However, these antidotes are still understudies and need to be fully approved. The assessment of DOACs is developing and several drug-specific calibrators have been developed to measure DOACs activity in plasma.
Heart, 1995
Objective-To determine the development of titres of streptokinase (SK) neutralising antibodies af... more Objective-To determine the development of titres of streptokinase (SK) neutralising antibodies after a single dose of SK, to establish when titres decrease to levels at which a second dose might be effective. Design-Analyses of blood samples taken from patients at intervals after SK administration. Setting-Australian public hospital. Patients-104 patients with acute myocardial infarction who were treated with SK and 27 controls who were not. Outcome measure-SK neutralising antibodies were measured once in each of the 27 controls and on 166 occasions in the 104 treated patients. Results-Titres of SK neutralising antibodies rose after SK administration but returned to control levels by 2 years. Conclusions-SK might be effective again as a thrombolytic agent as early as 2 years after a single dose. These results are at variance with most previously published data and the reasons for this are not clear. Data evaluating patency rates after standard doses of streptokinase in patients with increased titres of neutralising antibodies are necessary before re-exposure to streptokinase can be recommended.
Complementary Therapies in Medicine
BACKGROUND Passive heat therapies have been reported to have similar effects on the cardiovascula... more BACKGROUND Passive heat therapies have been reported to have similar effects on the cardiovascular system as exercise. Studies supporting these findings in healthy populations have predominantly been done with men using warm water immersions or traditional saunas, rather than newer infrared-based saunas. OBJECTIVE To explore short-term thermal and cardiovascular responses in women using an infrared sauna as compared to moderate-intensity exercise. STUDY DESIGN Randomised controlled crossover trial with balanced allocations. SETTING Brisbane, Australia (August 2019 - March 2020) PARTICIPANTS: Ten healthy women (36 ± 9 years) INTERVENTIONS: 45minutes of resting, infrared sauna or indoor bicycling PRIMARY OUTCOME MEASURES: tympanic/skin temperatures; respiratory rate; blood pressure; arterial stiffness; heart rate variability RESULTS: Tympanic temperatures were elevated during infrared sauna as compared to both control (mean diff = +1.05oC ± SEM 0.12 oC, 95% C.I.: 0.73 - 1.36, p < 0.0005) and exercise (mean diff = +0.79oC ± SEM 0.12 oC, 95% C.I.: 0.49 - 1.08, p<0.0005). Respiratory rates were higher during exercise as compared to both control (mean diff = +7.66 ± SEM 1.37, 95% C.I.: 4.09 - 11.23, p < 0.0005) and infrared sauna (mean diff = +6.66 ± SEM 1.33, 95% C.I.: 3.20 - 10.11, p < 0.0005). No significant differences in non-invasive measures of blood pressure, arterial stiffness or heart rate variability were detected between any of the interventions. CONCLUSIONS These findings suggest the physiological effects of infrared sauna bathing are underpinned by thermoregulatory-induced responses, more so than exercise-mimetic cardiorespiratory or cardiovascular activations.
The Flipped Classroom
" in Chap. 9. The erratum chapter and the book have been updated with the change.
American Journal of Clinical Pathology
The Sysmex NE-8000® is a new, fully automated hematology analyzer capable of providing a five-par... more The Sysmex NE-8000® is a new, fully automated hematology analyzer capable of providing a five-part white blood cell differential count and identifying abnormal specimens. This instrument was evaluated on 5,000 consecutive blood specimens and compared to the Coulter S Plus-IV® analyzer and manual differential cell counts to determine the efficacy of its five-cell differential and screening capabilities. There was a high correlation between the commercial counters for the standard parameters, white blood cell count, red blood cell count, hemoglobin level, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin concentration, and platelet count (r > 0.95), except for the mean corpuscular hemoglobin concentration (r = 0.51), for which the NE-8000 was considered the more accurate measurement. Precision and linearity studies were excellent. The white blood cell count, red blood cell count, hemoglobin level, and platelet count were reproducible on specimens stored at 4°C or room temperature for 72 hours and the differential counts were reproducible for 12 hours. The correlations between automated and manual counts for neutrophils, eosinophils, basophils, and lymphocytes were excellent: r = 0.912, 0.945, 0.332, and 0.964, The Sysmex NE-8000® (Toa Medical Electronics, Kobe Japan) is a recently developed, high throughput automated hematology analyzer with the capacity to test 23 parameters at a maximal rate of 120 samples per hour in automatic mode. The instrument tests white blood cell count (WBC); red blood cell (RBC) count; hemoglobin concentration (HGB); hematocrit (HCT); mean corpuscular volume (MCV); mean corpuscular hemoglobin (MCH); mean corpuscular hemoglobin concentration (MCHC);
The Flipped Classroom, 2017
The importance of STEM (Science, Technology, Engineering and Mathematics) disciplines for the fut... more The importance of STEM (Science, Technology, Engineering and Mathematics) disciplines for the future economic and social well-being of all Australians cannot be underestimated: 75% of the fastest growing global occupations require STEM skills and knowledge (Becker and Park in J STEM Edu 12(6), 2011). Increased participation in STEM-related tertiary education is fundamental to the economic and social well-being of the individual and the nation, yet the number and capacity of STEM graduates Australia produced from tertiary institutions is inadequate (OECD in Over-qualified or under-skilled: A review of existing literature. OECD, Paris, 2011). Attracting and retaining STEM tertiary students will rely upon approaches to learning and teaching that engage, motivate and inspire more diverse cohorts. As the Australian Chief Scientist notes: STEM disciplines are critical engines of innovation and growth. The future of the Australian economy will be underpinned by the number and calibre of STEM graduates and the academic staff leading them. We are at present falling short: something different has to be done, demanding a paradigm shift (Office of the Chief Scientist, Australia, 2012). This chapter discusses a recent initiative The RMIT Inclusive Teaching and Assessment Practices Project which was created to address the diverse needs of all learners across the university.
Journal of Biological Chemistry, 1998
Blood, 2014
Key Points CEACAM2 is a novel platelet immunoreceptor. CEACAM2 negatively regulates platelet-coll... more Key Points CEACAM2 is a novel platelet immunoreceptor. CEACAM2 negatively regulates platelet-collagen interactions and thrombus growth and stability in vitro, in vivo and CLEC-2 pathways.
Journal of Science Education and Technology, 2016
Abstract A common trend in higher education is the “flipped” classroom, which facilitates active ... more Abstract A common trend in higher education is the “flipped” classroom, which facilitates active learning during class. The flipped approach to teaching was instituted in a haematology ‘major’ class and the students’ attitudes and preferences for the teaching materials were surveyed. The curriculum design was explicit and involved four major components (1) the preparation of the students; (2) the weekly pre-class work; (3) the in-class active learning strategies and (4) closing the learning loop using formative quizzes. Each of these components is discussed in detail and was informed by sound pedagogical strategies. Several different sources of information and several freely available software tools to engage the students are discussed. Two iterations are reported here, with improved pass rate for the final examination from 47 to 48 % in the traditional class to 56–65 % in the flipped classroom approach. The majority of students (93 and 89 %) came to the class prepared, after viewing the screencasts and engaged fully with the activities within the face-to-face time. The students perceived that solving case studies (93 %) was the most beneficial activity for their learning and this was closely followed by the production of essay plans (71 %). The majority of students recommended that this approach be repeated the following year (69 and 75 %).
Clinical and Applied Thrombosis/Hemostasis, 1997
Alternative anticoagulation in patients with heparin-induced thrombocytopenia (HIT) is often prob... more Alternative anticoagulation in patients with heparin-induced thrombocytopenia (HIT) is often problematic. The relatively high cross-reactivity rate reported for the low-molecular-weight heparins (LMWH) has discouraged their use in this setting. This study has investigated the safety of using the LMWH Fragmin, based on a negative heparin-dependent platelet aggregation test using the latter, in patients with proven HIT. Fifty-three evaluable patients with clinical and laboratory evidence of HIT were evaluated for cross-reactivity with Fragmin using a Fragmin-dependent platelet aggregation test. In 20 of 38 patients who showed no in vitro cross-reactivity. Fragmin was substituted for unfractionated heparin. The outcome of these 20 patients was evaluated and compared to that of the remaining 33 patients, in whom anticoagulates were ceased or warfarin or Orgaran was used. Eighteen of 20 patients treated with Fragmin increased their platelet count by ≥50 x 109/l from a mean nadir of 57.9 ...
American Journal of Clinical Pathology
Two hundred consecutive patients who were referred for evaluation of spontaneous or recurrent thr... more Two hundred consecutive patients who were referred for evaluation of spontaneous or recurrent thrombosis were investigated for possible hypercoagulable states to determine the relative frequencies of these conditions in the Australian population and to identify features that would indicate which patients should be investigated with the expensive battery of tests for hypercoagulable states. Thirty-two percent were found to have prolongation of the postvenous occlusion euglobulin clot lysis time (PVO-ELT), 32% were found to have elevated levels of plasminogen activator inhibitor-1 (PAI) and 66% were found to have reduced release of tissue plasminogen activator (tPA). Antiphospholipid antibodies were found in 12%. Hereditary antithrombin III deficiency was found in 2%. Hereditary deficiency of the naturally-occurring anti-coagulant factors protein C and protein S was found in 2%. Age, sex, site of thrombosis (venous or arterial), or presence of a family history was not helpful in predi...
Journal of Science Education and Technology, Jan 20, 2016
A common trend in higher education is the "flipped" classroom, which facilitates active learning ... more A common trend in higher education is the "flipped" classroom, which facilitates active learning during class. The flipped approach to teaching was instituted in a haematology 'major' class and the students' attitudes and preferences for the teaching materials were surveyed. The curriculum design was explicit and involved four major components 1) the preparation of the students; 2) the weekly pre-class work; 3) the in-class active learning strategies and 4) closing the learning loop using formative quizzes. Each of these components is discussed in detail and was informed by sound pedagogical strategies. Several different sources of information and several freely available software tools to engage the students are discussed. Two iterations are reported here, with improved pass rate for the final examination from 47-48% in the traditional class to 56-65% in the flipped classroom approach. The majority of students (93% and 89%) came to the class prepared, after viewing the screencasts and engaged fully with the activities within the face-to-face time. The students perceived that solving case studies (93%) was the most beneficial activity for their learning and this was closely followed by the production of essay plans (71%). The majority of students recommended that this approach be repeated the following year (69% and 75%).
Thrombosis Research, Apr 1, 2019
BAK BCL 2 homologous antagonist killer BCL B-cell lymphoma BAX BCL 2 associated X protein BM Bone... more BAK BCL 2 homologous antagonist killer BCL B-cell lymphoma BAX BCL 2 associated X protein BM Bone marrow BSS Bernard-Soulier Syndrome cAMP Cyclic adenosine monophosphate CEACAM Carcinoembryonic antigen-releated cell adhesion molecule Ceacam1 Murine ceacam1 gene Ceacam2 Murine ceacam2 gene CRD-like Carbohydrate-like recognition domain CRPs Collagen-related peptide CVD Cardiovascular disease CLEC-2 C-type lectin-like receptor 2 CIB1 Calcium and integrin binding protein 1
Blood, Oct 9, 2014
• CEACAM2 is a novel platelet immunoreceptor. • CEACAM2 negatively regulates platelet-collagen in... more • CEACAM2 is a novel platelet immunoreceptor. • CEACAM2 negatively regulates platelet-collagen interactions and thrombus growth and stability in vitro, in vivo and CLEC-2 pathways. Carcinoembryonic antigen-related cell adhesion molecule-2 (CEACAM2) is a cell-surface glycoprotein expressed on blood, epithelial, and vascular cells. CEACAM2 possesses adhesive and signaling properties mediated by immunoreceptor tyrosine-based inhibitory motifs. In this study, we demonstrate that CEACAM2 is expressed on the surface and in intracellular pools of platelets. Functional studies of platelets from Ceacam2 2/2deficient mice (Cc2 2/2) revealed that CEACAM2 serves to negatively regulate collagen glycoprotein VI (platelet) (GPVI)-FcRg-chain and the C-type lectinlike receptor 2 (CLEC-2) signaling. Cc2 2/2 platelets displayed enhanced GPVI and CLEC-2-selective ligands, collagen-related peptide (CRP), collagen, and rhodocytin (Rhod)-mediated platelet aggregation. They also exhibited increased adhesion on type I collagen, and hyperresponsive CRP and CLEC-2-induced a and dense granule release compared with wild-type platelets. Furthermore, using intravital microscopy to ferric chloride (FeCl 3)-injured mesenteric arterioles and laser-induced injury of cremaster muscle arterioles, we herein show that thrombi formed in Cc2 2/2 mice were larger and more stable than wild-type controls in vivo. Thus, CEACAM2 is a novel platelet immunoreceptor that acts as a negative regulator of platelet GPVI-collagen interactions and of ITAM receptor CLEC-2 pathways.
Platelets, May 9, 2016
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is an Ig-ITIM superfamily mem... more Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is an Ig-ITIM superfamily member that regulates integrin αIIbβ3 function. We hypothesized that its twin protein, CEACAM2, exerts a similar physiologic role in murine platelets. CEACAM2-deficient mice (Cc2(-/-)) displayed prolonged tail bleeding times and increased volume of blood loss. Cc2(-/-) platelets have moderate integrin αIIbβ3-mediated functional defects with impaired kinetics of platelet spreading on fibrinogen and type I collagen and delayed kinetics in the retraction of fibrin clots in vitro. This functional integrin αIIbβ3 defect could not be attributed to altered integrin αIIbβ3 expression. Cc2(-/-) platelets displayed normal &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;inside-out&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; signaling properties as demonstrated by normal agonist-induced binding of soluble fluorescein isothiocyanate (FITC)-fibrinogen and JON/A antibody binding. This data provides direct evidence that disruption of CEACAM2 induces a moderate integrin αIIbβ3-mediated platelet function defect, and that CEACAM2 is essential to maintain a normal integrin αIIbβ3-mediated platelet function.
International Journal of Hygiene and Environmental Health
International Journal of Osteopathic Medicine
Blood, 1996
This report describes the effect of pegylated recombinant human megakaryocyte growth and developm... more This report describes the effect of pegylated recombinant human megakaryocyte growth and development factor (PEG-rHuMGDF) on platelet production and platelet function in humans. Subjects with advanced solid tumors received PEG-rHuMGDF daily for up to 10 days. There was no increase in circulating platelet count at doses of 0.03 or 0.1 microgram/kg/d by day 12 of study. At doses of 0.3 and 1.0 microgram/kg/d there was a threefold median increase (maximum 10-fold) in platelet count by day 16. The platelets produced in vivo in response to PEG-rHuMGDF showed unchanged aggregation and adenosine triphosphate (ATP)-release responses in in vitro assays. Tests included aggregation and release of ATP in response to adenosine diphosphate (ADP) (10, 5, 2.5, and 1.25 mumol/L), collagen (2 micrograms/mL), thrombin-receptor agonist peptide (TRAP, 10 mumol/L) and ristocetin (1.5 mg/mL). Administration of aspirin to an individual with platelet count of 1,771 x 10(3)/L resulted in the typical aspirin-...
Clinica Chimica Acta, 2019
Background-aim Biochemically Vitamin B12 deficiency causes decreased methionine synthase and L-Me... more Background-aim Biochemically Vitamin B12 deficiency causes decreased methionine synthase and L-Methylmalonyl-CoA Mutase activity, which results in accumulation of Homocysteine (HCY), Methylmalonic acid (MMA) and propionyl-l-carnitine (C3). Infant vitamin B12 deficiency is usually caused by maternal deficiency due to vegetarian diet or pernicious anemia (intrinsic factor antibodies (FI) and/or parietal cell antibodies (anti-CP)).The early recognition and treatment in infant deficiency can prevent potentially serious and irreversible neurologic damage. The maternal vitamin B12 deficiency can be detected through the expanded newborn screening by tandem mass spectrometry (MS/MS) with the high levels of C3, C3/C2 (propionyl-lcarnitine /acetyl-l-carnitine) ratio in the dried blood spot sample (DBS) of newborns. The aim of this study is to evaluate the prevalence of maternal cobalamine deficiency detected through expanded newborn screening.
Hematology & Transfusion International Journal, 2017
Thrombotic disorders are treated mostly with Heparin and Warfarin in cases requiring anticoagulat... more Thrombotic disorders are treated mostly with Heparin and Warfarin in cases requiring anticoagulation drugs. Due to limitations to these drugs including risk of bleeding and need for regular laboratory monitoring several Direct oral anticoagulants (DOACs) have been developed and shown to have more advantages than Warfarin. Nevertheless, these drugs are limited to use in some circumstances. As other anticoagulants, these drugs need to be managed and controlled to cope their over activities. Subsequently, many antidotes have been developed to reverse these DOACs. However, these antidotes are still understudies and need to be fully approved. The assessment of DOACs is developing and several drug-specific calibrators have been developed to measure DOACs activity in plasma.
Heart, 1995
Objective-To determine the development of titres of streptokinase (SK) neutralising antibodies af... more Objective-To determine the development of titres of streptokinase (SK) neutralising antibodies after a single dose of SK, to establish when titres decrease to levels at which a second dose might be effective. Design-Analyses of blood samples taken from patients at intervals after SK administration. Setting-Australian public hospital. Patients-104 patients with acute myocardial infarction who were treated with SK and 27 controls who were not. Outcome measure-SK neutralising antibodies were measured once in each of the 27 controls and on 166 occasions in the 104 treated patients. Results-Titres of SK neutralising antibodies rose after SK administration but returned to control levels by 2 years. Conclusions-SK might be effective again as a thrombolytic agent as early as 2 years after a single dose. These results are at variance with most previously published data and the reasons for this are not clear. Data evaluating patency rates after standard doses of streptokinase in patients with increased titres of neutralising antibodies are necessary before re-exposure to streptokinase can be recommended.
Complementary Therapies in Medicine
BACKGROUND Passive heat therapies have been reported to have similar effects on the cardiovascula... more BACKGROUND Passive heat therapies have been reported to have similar effects on the cardiovascular system as exercise. Studies supporting these findings in healthy populations have predominantly been done with men using warm water immersions or traditional saunas, rather than newer infrared-based saunas. OBJECTIVE To explore short-term thermal and cardiovascular responses in women using an infrared sauna as compared to moderate-intensity exercise. STUDY DESIGN Randomised controlled crossover trial with balanced allocations. SETTING Brisbane, Australia (August 2019 - March 2020) PARTICIPANTS: Ten healthy women (36 ± 9 years) INTERVENTIONS: 45minutes of resting, infrared sauna or indoor bicycling PRIMARY OUTCOME MEASURES: tympanic/skin temperatures; respiratory rate; blood pressure; arterial stiffness; heart rate variability RESULTS: Tympanic temperatures were elevated during infrared sauna as compared to both control (mean diff = +1.05oC ± SEM 0.12 oC, 95% C.I.: 0.73 - 1.36, p < 0.0005) and exercise (mean diff = +0.79oC ± SEM 0.12 oC, 95% C.I.: 0.49 - 1.08, p<0.0005). Respiratory rates were higher during exercise as compared to both control (mean diff = +7.66 ± SEM 1.37, 95% C.I.: 4.09 - 11.23, p < 0.0005) and infrared sauna (mean diff = +6.66 ± SEM 1.33, 95% C.I.: 3.20 - 10.11, p < 0.0005). No significant differences in non-invasive measures of blood pressure, arterial stiffness or heart rate variability were detected between any of the interventions. CONCLUSIONS These findings suggest the physiological effects of infrared sauna bathing are underpinned by thermoregulatory-induced responses, more so than exercise-mimetic cardiorespiratory or cardiovascular activations.
The Flipped Classroom
" in Chap. 9. The erratum chapter and the book have been updated with the change.
American Journal of Clinical Pathology
The Sysmex NE-8000® is a new, fully automated hematology analyzer capable of providing a five-par... more The Sysmex NE-8000® is a new, fully automated hematology analyzer capable of providing a five-part white blood cell differential count and identifying abnormal specimens. This instrument was evaluated on 5,000 consecutive blood specimens and compared to the Coulter S Plus-IV® analyzer and manual differential cell counts to determine the efficacy of its five-cell differential and screening capabilities. There was a high correlation between the commercial counters for the standard parameters, white blood cell count, red blood cell count, hemoglobin level, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin concentration, and platelet count (r > 0.95), except for the mean corpuscular hemoglobin concentration (r = 0.51), for which the NE-8000 was considered the more accurate measurement. Precision and linearity studies were excellent. The white blood cell count, red blood cell count, hemoglobin level, and platelet count were reproducible on specimens stored at 4°C or room temperature for 72 hours and the differential counts were reproducible for 12 hours. The correlations between automated and manual counts for neutrophils, eosinophils, basophils, and lymphocytes were excellent: r = 0.912, 0.945, 0.332, and 0.964, The Sysmex NE-8000® (Toa Medical Electronics, Kobe Japan) is a recently developed, high throughput automated hematology analyzer with the capacity to test 23 parameters at a maximal rate of 120 samples per hour in automatic mode. The instrument tests white blood cell count (WBC); red blood cell (RBC) count; hemoglobin concentration (HGB); hematocrit (HCT); mean corpuscular volume (MCV); mean corpuscular hemoglobin (MCH); mean corpuscular hemoglobin concentration (MCHC);
The Flipped Classroom, 2017
The importance of STEM (Science, Technology, Engineering and Mathematics) disciplines for the fut... more The importance of STEM (Science, Technology, Engineering and Mathematics) disciplines for the future economic and social well-being of all Australians cannot be underestimated: 75% of the fastest growing global occupations require STEM skills and knowledge (Becker and Park in J STEM Edu 12(6), 2011). Increased participation in STEM-related tertiary education is fundamental to the economic and social well-being of the individual and the nation, yet the number and capacity of STEM graduates Australia produced from tertiary institutions is inadequate (OECD in Over-qualified or under-skilled: A review of existing literature. OECD, Paris, 2011). Attracting and retaining STEM tertiary students will rely upon approaches to learning and teaching that engage, motivate and inspire more diverse cohorts. As the Australian Chief Scientist notes: STEM disciplines are critical engines of innovation and growth. The future of the Australian economy will be underpinned by the number and calibre of STEM graduates and the academic staff leading them. We are at present falling short: something different has to be done, demanding a paradigm shift (Office of the Chief Scientist, Australia, 2012). This chapter discusses a recent initiative The RMIT Inclusive Teaching and Assessment Practices Project which was created to address the diverse needs of all learners across the university.
Journal of Biological Chemistry, 1998
Blood, 2014
Key Points CEACAM2 is a novel platelet immunoreceptor. CEACAM2 negatively regulates platelet-coll... more Key Points CEACAM2 is a novel platelet immunoreceptor. CEACAM2 negatively regulates platelet-collagen interactions and thrombus growth and stability in vitro, in vivo and CLEC-2 pathways.
Journal of Science Education and Technology, 2016
Abstract A common trend in higher education is the “flipped” classroom, which facilitates active ... more Abstract A common trend in higher education is the “flipped” classroom, which facilitates active learning during class. The flipped approach to teaching was instituted in a haematology ‘major’ class and the students’ attitudes and preferences for the teaching materials were surveyed. The curriculum design was explicit and involved four major components (1) the preparation of the students; (2) the weekly pre-class work; (3) the in-class active learning strategies and (4) closing the learning loop using formative quizzes. Each of these components is discussed in detail and was informed by sound pedagogical strategies. Several different sources of information and several freely available software tools to engage the students are discussed. Two iterations are reported here, with improved pass rate for the final examination from 47 to 48 % in the traditional class to 56–65 % in the flipped classroom approach. The majority of students (93 and 89 %) came to the class prepared, after viewing the screencasts and engaged fully with the activities within the face-to-face time. The students perceived that solving case studies (93 %) was the most beneficial activity for their learning and this was closely followed by the production of essay plans (71 %). The majority of students recommended that this approach be repeated the following year (69 and 75 %).
Clinical and Applied Thrombosis/Hemostasis, 1997
Alternative anticoagulation in patients with heparin-induced thrombocytopenia (HIT) is often prob... more Alternative anticoagulation in patients with heparin-induced thrombocytopenia (HIT) is often problematic. The relatively high cross-reactivity rate reported for the low-molecular-weight heparins (LMWH) has discouraged their use in this setting. This study has investigated the safety of using the LMWH Fragmin, based on a negative heparin-dependent platelet aggregation test using the latter, in patients with proven HIT. Fifty-three evaluable patients with clinical and laboratory evidence of HIT were evaluated for cross-reactivity with Fragmin using a Fragmin-dependent platelet aggregation test. In 20 of 38 patients who showed no in vitro cross-reactivity. Fragmin was substituted for unfractionated heparin. The outcome of these 20 patients was evaluated and compared to that of the remaining 33 patients, in whom anticoagulates were ceased or warfarin or Orgaran was used. Eighteen of 20 patients treated with Fragmin increased their platelet count by ≥50 x 109/l from a mean nadir of 57.9 ...
American Journal of Clinical Pathology
Two hundred consecutive patients who were referred for evaluation of spontaneous or recurrent thr... more Two hundred consecutive patients who were referred for evaluation of spontaneous or recurrent thrombosis were investigated for possible hypercoagulable states to determine the relative frequencies of these conditions in the Australian population and to identify features that would indicate which patients should be investigated with the expensive battery of tests for hypercoagulable states. Thirty-two percent were found to have prolongation of the postvenous occlusion euglobulin clot lysis time (PVO-ELT), 32% were found to have elevated levels of plasminogen activator inhibitor-1 (PAI) and 66% were found to have reduced release of tissue plasminogen activator (tPA). Antiphospholipid antibodies were found in 12%. Hereditary antithrombin III deficiency was found in 2%. Hereditary deficiency of the naturally-occurring anti-coagulant factors protein C and protein S was found in 2%. Age, sex, site of thrombosis (venous or arterial), or presence of a family history was not helpful in predi...