Easter Nwokah | RIVERS STATE UNIVERSITY OF SCIENCE AND TECHNOLOGY (original) (raw)

Papers by Easter Nwokah

Asian Journal of Research in Infectious Diseases

This study aims at determining the prevalence of Vibrio cholerae among the people of Andoni Local... more This study aims at determining the prevalence of Vibrio cholerae among the people of Andoni Local Government. One hundred stool samples were collected after administering well-structured questionnaire and were analysed with standard microbiological techniques which includes; macroscopy, microscopy, culture on thiosulphate-citrate-bile salts-sucrose agar, Gram staining and biochemical tests (indole, motility, catalase and oxidase tests). Results showed a prevalence of 30% of Vibrio cholerae. Vibrio parahaemolyticus was also isolated with a prevalence rate of 42%. Among the predisposing factors, the prevalence rate of vibrio species based on demographic data showed positive with 77.8% of those tested within the age range 11-15years, 80% of those tested within the age range 16-20years while 6-10years and 26-30years had 0%. Among the sexes, 78.2% of the females and 64.4% of males tested were positive. In the religion category, 75.8% positive among Christians tested and 33.3% among other...

Microbiology Research Journal International

Mosquitoes are a menace for millions of people around the world, they are vectors for destructive... more Mosquitoes are a menace for millions of people around the world, they are vectors for destructive microorganisms which cause diseases like Malaria, Dengue fever and Lymphatic filariasis, affecting people in developing countries and areas with tropical climates. Anopheles, a predominant genus transmits malaria, and the World Health Organization have shown that 350,000 lives especially children and pregnant women are lost annually by it. The aim of this study is to determine the effect of eosin solution exposed to sunlight on larvae of Anopheles mosquito. The use of control method was aid at eliminating the larva stages of the mosquito life cycle. A total of one hundred and twenty (120) Anopheles mosquito larvae were harvested using dipper with handle and net from drainages at Eagle Island and Rivers State University both in Port Harcourt. Five different concentrations of Eosin solutions were prepared in volumes of 1000 microlitre (µl), 800µl, 600µl, 400µl, 200µl after a stock solutio...

Microbiology Research Journal International

Background: Bitter leaf and palm oil combination in treating diarrhoea is common in Africa. The a... more Background: Bitter leaf and palm oil combination in treating diarrhoea is common in Africa. The aim of this study is to determine the synergistic and antimicrobial potential of Vernonia amygdalina and Elaeis guineensis on bacteria isolates from diarrheic stool. Methodology: One hundred and fifty (150) diarrheic stool samples from the Diagnostic Laboratory in Rivers State University Teaching Hospital (RSUTH), Port Harcourt, were inoculated in Selenite F broth, MacConkey, Nutrient and Salmonella-Shigella agar plates using standard methods. Fresh and dry bitter leaf extracts were prepared by mashing, weighing and dissolving 100 grams(g) and 150 g concentrations of each in 100 milliliters(mls) of ethanol and sterile distilled water respectively. Undiluted mixture of bitter leaf and palm oil were prepared by mixing 2 mls of each in a sterile beaker. Zero-point one milliliter (0.1ml) of each undiluted extract, their mixture, ethanol and palm oil were separately dispensed into appropriatel...

Journal of Advances in Microbiology, 2022

Aim: To isolate and detect the antimicrobial resistance of some Gram-negative Bacilli Isolated fr... more Aim: To isolate and detect the antimicrobial resistance of some Gram-negative Bacilli Isolated from various sources in Port Harcourt, Nigeria. Study Design: Cross-sectional study. Place and Duration of Study: University of Port Harcourt Teaching Hospital, Trans-Amadi and Rumuodomaya Slaughters and Bob-D Ventures Poultry Farms, all in Port Harcourt, Nigeria, between July and December, 2019. Methodology: This study was conducted at Port Harcourt and Obio/Akpor local government areas of Rivers State, Nigeria from 2019-2020. Hospital wastewater was taken from different sections at the two University Teaching Hospitals- University of Port Harcourt and Rivers State University Teaching Hospitals. Abattoir effluent water samples were taken at different sites from Trans-Amadi and Rumuodumaya Abattoirs. Chicken cloaca samples as well as Hand swab samples of Butchers were collected at the two Abattoirs. All samples were processed following standard procedures and identified organisms were ass...

South Asian Journal of Research in Microbiology, 2021

Aim: To determine extended-spectrum β-lactamase enzymes (ESBLS) and slime production of some Gram... more Aim: To determine extended-spectrum β-lactamase enzymes (ESBLS) and slime production of some Gram-negative Bacilli isolated from human, animal and environmental sources in Port Harcourt, Nigeria Study design: Cross-sectional study. Place and Duration of Study: University of Port Harcourt Teaching Hospital, Trans-Amadi and Rumuodomaya Slaughters and Bob-D Ventures Poultry Farms, all in Port Harcourt, Nigeria, between July and December, 2019. Methodology: This study was conducted at Port Harcourt and Obio/Akpor local government areas of Rivers State, Nigeria from 2019-2020. Hospital wastewater was taken from different sections at the two University Teaching Hospitals- University of Port Harcourt and Rivers State University Teaching Hospitals. Abattoir effluent water samples were taken at different sites from Trans-Amadi and Rumuodumaya Abattoirs. Chicken cloaca samples as well as Hand swab samples of Butchers were collected at the two Abattoirs. All samples were processed following s...

World Journal of Pharmaceutical and life sciences, Apr 8, 2005

Biomedical Journal of Scientific & Technical Research, 2020

The study was aimed at evaluating the relationship between platelets count, bacterial vaginosis a... more The study was aimed at evaluating the relationship between platelets count, bacterial vaginosis and immune response in cervical human Papillomavirus positive women in Bayelsa State. Cervical smears were collected from apparently healthy subjects attending clinics in Bayelsa State. Consensus primers GP5+/6+ and MY09/11 were used to detect HPV DNA in cervical smears and further subjected to multiplex PCR for molecular typing of HPV subtypes. Nugent method was used for scoring of bacterial vaginoses based on gram staining reaction. Interleukins were studied using sandwich ELISA kits. The prevalence of bacterial vaginosis among HPV positive, HPV negative and high-risk HPV was 35.9% and 18.2% and 51.7% respectively. Bacterial vaginosis expressed statistically significant relationship with high risk HPV (OR=9.64, P=0.002, 95%CI= 1.08-86.26).Interleukin 4 (IL-4), IL-6, IL-8, IL-10, IL-12, IL-17, IL-18, Tumor Necrotic Factor (TNF) and Interferon Gamma (INF-γ) were equally higher in HPV positive participants compared with negative participants and statistically significant. There exists a statistically significantly decrease in platelets count (227.8±57) of HPV positive participants compared with negative participants (259.3±57), (X2=6.8, P=0.01) as well as neutrophils count of 45.2±8.5 of HPV positive compared with negative participants (50.6±8.4), (X 2 =2.9, P=0.01).White blood cell count, monocytes and packed cell volume were equally higher in positive cases compared with negative cases. Bacterial Vaginosis showed a strong relationship with high risk human Papillomavirus, indicating that a healthy vaginal flora will not only protect a woman from the acquisition of sexually transmitted viruses but also high-risk HPV. Interleukins participate in HPV infection and association interferon levels were depleted in HPV infected participants. Thrombocytes and neutrophils are cellular mediators in genital HPV infection. It is recommended therefore that women exposed to high-risk HPV should be screened for bacterial vaginosis, with concomitant estimations of levels of platelets as well as inflammatory cytokines.

Mary Slessor Journal of Medicine, 2009

No Abstract. Mary Slessor Journal of Medicine Vol. 8 (1) 2008: pp. 27-3

South Asian Journal of Research in Microbiology

Aim: The aim of this study was to profile the anti-microbial resistant of Mycobacterial strains c... more Aim: The aim of this study was to profile the anti-microbial resistant of Mycobacterial strains causing cluster pulmonary infections in Bayelsa State, Nigeria. Study Design: Cross sectional facility based. Place and Duration: This study was carried out in all the in tuberculosis centers in all the eight local government area in Bayelsa State, Nigeria between January 2019 to February 2021. Materials and Methods: A total of 102 tuberculosis patients who had been previously diagnosed of Tuberculosis (both new and old cases) participated in the study. Two sputum samples were collected from each subject in a wide mouth screw cape container and falcon tube container respectively. The first was used for GenXpert technique while the second was used for culture on Lowenstein Jensen solid Media. The visible and confirmed Mycobacterium growth was subjected to Line Probe Assay (MTBDRplus assay) and genetic sequencing. The DNA of the isolates where extracted using Genolyse method. The extracted ...

International Journal of Pathogen Research

Aim: To determine the prevalent rates of Escherichia coli O157: H7 and non-O157 strains in of iso... more Aim: To determine the prevalent rates of Escherichia coli O157: H7 and non-O157 strains in of isolates from meat, human stool and abattoir waste water, collected from selected sources in Port Harcourt, Nigeria. Study Design: Case-controlled study. Place and Duration of Study: Selected places in Port Harcourt, Rivers State, Nigeria, between November, 2020 to November, 2021. Methodology: Three hundred and forty -nine (349) samples were analyzed, 80 meat and 63 waste waters from five abattoirs cited in the city, 46 meat samples from five selected sites sold by roadside butchers, 109 patient stool samples and 30 stool samples from food sellers, in addition to 20 stool samples from healthy subjects and 1 commercial bottled water which served as control samples. Tryptone soya broth (TSB) as an enrichment media, selective agar media namely; Eosin methylene blue agar, cefixime tellurite-sorbitol MacConkey agar (CT-SMAC), Chromagar STEC were used to isolate STEC from the samples and serology...

Asian Research Journal of Gynaecology and Obstetrics, Apr 20, 2019

Journal of Complementary and Alternative Medical Research, 2021

Almost every part of a banana plant has use in medicine. Increased bacterial resistance to the co... more Almost every part of a banana plant has use in medicine. Increased bacterial resistance to the conventional antibiotics has led to research into the development of alternative treatments to conventional antibiotics. This study focuses on the antimicrobial properties of banana peels against selected isolates from Urinary Tract Infection sample. The isolates from cultures further analysed with agarose gel electrophoresis for the presence of 16SrRNA and Phylogenetic analysis revealed Staphylococcus sciuri strain, a coagulase‐negative species, Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae and Proteus mirabilis. Fresh unripe and dry unripe were used to determine the antimicrobial activities. A measure of 150grams of both fresh unripe and dry unripe was extracted with 100ml of water solvent (sterile) respectively for about 2days. The solvent extracts were concentrated separately under reduced pressure, 10g of each concentrated solvent extracts were dissolved in 5ml of st...

Asian Journal of Research in Infectious Diseases, 2022

Banana peels refers to the outer coverage of the banana fruit. Apart from serving as a food for m... more Banana peels refers to the outer coverage of the banana fruit. Apart from serving as a food for man and animals, the banana plant also has medicinal values. Urinary Tract Infection is an infection that affects any parts of the urinary system (upper or lower). Its treatment and diagnosis are carried out with urine sample and antibiotics. Increased bacterial resistance to the conventional antibiotics has led to the development of alternative treatment for bacterial disease infections. This study is aimed at assessing the antimicrobial properties of fresh unripe and dried unripe banana peels against selected isolates from Urinary Tract Infection sample. The isolates from culture were further identified using biochemical tests. Fresh unripe and dry unripe were used to determine the antimicrobial activities. A measure of 150 grams of both fresh unripe and dry unripe was extracted with 100ml of 80% Ethanol solvent for about 2 days. The solvent extracts were concentrated separately under r...

Sahel Medical Journal, 2011

... Microbial Profile of wound infections in a Nigerian Teaching Hospital. MBO Ayodele, SD Abbey,... more ... Microbial Profile of wound infections in a Nigerian Teaching Hospital. MBO Ayodele, SD Abbey, EG Nwokah, OK Obunge, GCS Omakwele. Abstract. Background: This study set out to investigate the aerobic and facultative anaerobic ...

International Journal of Pathogen Research

Aim: To assess the Extended Spectrum Beta-Lactamase (ESBL) resistant genes in Salmonella spp isol... more Aim: To assess the Extended Spectrum Beta-Lactamase (ESBL) resistant genes in Salmonella spp isolated from ready-to-eat foods and food handlers in Port Harcourt Metropolis, Nigeria. Study Design: A cross-sectional study. Place and Duration of Study: This study was conducted in selected places in Port Harcourt, between November 2019 and June 2021. Methodology: A total of 350 food specimens and 230 food handlers were collected and the social demographic information were collected using a questionnaire survey. The samples were analysed for contamination with Salmonella species using conventional and molecular methods. Salmonella species were isolated from samples using Salmonella-Shigella agar (SSA), Xylose-lysine desoxycholate agar (XLD), MacConkey agar (MA), Blood agar (BA) after pre-enrichment and enrichment method has been done using peptone water broth and Selenite F. Salmonella Chromogenic medium (SCM) was also used to confirm the isolate. Antibiotic susceptibility patterns of t...

American Journal of Biomedical Sciences

Infections caused by Klebsiella sp. are increasingly becoming difficult to treat partly due to th... more Infections caused by Klebsiella sp. are increasingly becoming difficult to treat partly due to the rise in trend of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella sp. This study aimed to detect the prevalence of ESBL-producing Klebsiella sp. and SHV, TEM, CTX-M, and OXA beta-lactamase encoding genes in isolates of Klebsiella sp. from two healthcare facilities in Port Harcourt. A cross-sectional study of 146 clinical specimens was analyzed using standard bacteriological identification techniques. ESBL-producing Klebsiella sp. was identified using clinical laboratory standard institute standards. Beta-lactamase genes SHV, TEM, OXA, and CTXM were extracted and amplified. Of the 146 clinical specimens; 47 (32.2%) were from males and 99 (67.8%) females. The number of Klebsiella sp. isolated from the female samples 11 (73.3%) was more than 2-fold higher than those isolated from male samples 4 (26.7%). Eighty different bacterial isolates were obtained from which 15 (18.8%) we...

African Journal of Microbiology Research, Apr 14, 2018

The re-emergence and increase in the prevalence of tuberculosis cases and multidrug resistant str... more The re-emergence and increase in the prevalence of tuberculosis cases and multidrug resistant strains is of public health concern. The conventional Acid Fast Bacilli detection tool is weak in the current disease trend especially in children, patients with human immunodeficiency virus (HIV) and low bacterial load. The aim of this research was to assess effectiveness of the Gene Xpert MTB/RIF assay and Microscopy in detectiing Mycobacterium tuberculosis (MTB) in sputum samples in Port Harcourt metropolis. A total of two hundred sputum samples were collected from tuberculosis (TB) suspected patients visiting the antiretroviral and directly observed short treatment clinics at

American Journal of Biomedical Sciences

Journal of Advances in Microbiology

Aim: This study aimed to assess the quality of drinking water in some hydrocarbon-impacted Ogoni ... more Aim: This study aimed to assess the quality of drinking water in some hydrocarbon-impacted Ogoni communities. Study Design: The study employ a cross-sectional and analytical design using stratified sampling method. Place and Duration of Study: Department of Medical Laboratory Science of Rivers State University, Giolee Global Resource Limited and Environmental Consultancy Services between March 2020 and March 2021. Methodology: Water samples were collected from (20 hydrocarbon- impacted communities) in the 4 Local Government Area (LGAs) of Ogoni land. These water samples were analyzed to determine the physicochemical, bacteriological, heavy metal and total petroleum hydrocarbon (TPH) parameters using standard methods and operational procedures. The data obtained were subjected to descriptive statistical analysis. The general linearized model (GIG) was used to generate analysis of variance (ANOVA) mean and standard error and arrange, statistically significant was set as p-value of .05...

Asian Journal of Research in Infectious Diseases

This study aims at determining the prevalence of Vibrio cholerae among the people of Andoni Local... more This study aims at determining the prevalence of Vibrio cholerae among the people of Andoni Local Government. One hundred stool samples were collected after administering well-structured questionnaire and were analysed with standard microbiological techniques which includes; macroscopy, microscopy, culture on thiosulphate-citrate-bile salts-sucrose agar, Gram staining and biochemical tests (indole, motility, catalase and oxidase tests). Results showed a prevalence of 30% of Vibrio cholerae. Vibrio parahaemolyticus was also isolated with a prevalence rate of 42%. Among the predisposing factors, the prevalence rate of vibrio species based on demographic data showed positive with 77.8% of those tested within the age range 11-15years, 80% of those tested within the age range 16-20years while 6-10years and 26-30years had 0%. Among the sexes, 78.2% of the females and 64.4% of males tested were positive. In the religion category, 75.8% positive among Christians tested and 33.3% among other...

Microbiology Research Journal International

Mosquitoes are a menace for millions of people around the world, they are vectors for destructive... more Mosquitoes are a menace for millions of people around the world, they are vectors for destructive microorganisms which cause diseases like Malaria, Dengue fever and Lymphatic filariasis, affecting people in developing countries and areas with tropical climates. Anopheles, a predominant genus transmits malaria, and the World Health Organization have shown that 350,000 lives especially children and pregnant women are lost annually by it. The aim of this study is to determine the effect of eosin solution exposed to sunlight on larvae of Anopheles mosquito. The use of control method was aid at eliminating the larva stages of the mosquito life cycle. A total of one hundred and twenty (120) Anopheles mosquito larvae were harvested using dipper with handle and net from drainages at Eagle Island and Rivers State University both in Port Harcourt. Five different concentrations of Eosin solutions were prepared in volumes of 1000 microlitre (µl), 800µl, 600µl, 400µl, 200µl after a stock solutio...

Microbiology Research Journal International

Background: Bitter leaf and palm oil combination in treating diarrhoea is common in Africa. The a... more Background: Bitter leaf and palm oil combination in treating diarrhoea is common in Africa. The aim of this study is to determine the synergistic and antimicrobial potential of Vernonia amygdalina and Elaeis guineensis on bacteria isolates from diarrheic stool. Methodology: One hundred and fifty (150) diarrheic stool samples from the Diagnostic Laboratory in Rivers State University Teaching Hospital (RSUTH), Port Harcourt, were inoculated in Selenite F broth, MacConkey, Nutrient and Salmonella-Shigella agar plates using standard methods. Fresh and dry bitter leaf extracts were prepared by mashing, weighing and dissolving 100 grams(g) and 150 g concentrations of each in 100 milliliters(mls) of ethanol and sterile distilled water respectively. Undiluted mixture of bitter leaf and palm oil were prepared by mixing 2 mls of each in a sterile beaker. Zero-point one milliliter (0.1ml) of each undiluted extract, their mixture, ethanol and palm oil were separately dispensed into appropriatel...

Journal of Advances in Microbiology, 2022

Aim: To isolate and detect the antimicrobial resistance of some Gram-negative Bacilli Isolated fr... more Aim: To isolate and detect the antimicrobial resistance of some Gram-negative Bacilli Isolated from various sources in Port Harcourt, Nigeria. Study Design: Cross-sectional study. Place and Duration of Study: University of Port Harcourt Teaching Hospital, Trans-Amadi and Rumuodomaya Slaughters and Bob-D Ventures Poultry Farms, all in Port Harcourt, Nigeria, between July and December, 2019. Methodology: This study was conducted at Port Harcourt and Obio/Akpor local government areas of Rivers State, Nigeria from 2019-2020. Hospital wastewater was taken from different sections at the two University Teaching Hospitals- University of Port Harcourt and Rivers State University Teaching Hospitals. Abattoir effluent water samples were taken at different sites from Trans-Amadi and Rumuodumaya Abattoirs. Chicken cloaca samples as well as Hand swab samples of Butchers were collected at the two Abattoirs. All samples were processed following standard procedures and identified organisms were ass...

South Asian Journal of Research in Microbiology, 2021

Aim: To determine extended-spectrum β-lactamase enzymes (ESBLS) and slime production of some Gram... more Aim: To determine extended-spectrum β-lactamase enzymes (ESBLS) and slime production of some Gram-negative Bacilli isolated from human, animal and environmental sources in Port Harcourt, Nigeria Study design: Cross-sectional study. Place and Duration of Study: University of Port Harcourt Teaching Hospital, Trans-Amadi and Rumuodomaya Slaughters and Bob-D Ventures Poultry Farms, all in Port Harcourt, Nigeria, between July and December, 2019. Methodology: This study was conducted at Port Harcourt and Obio/Akpor local government areas of Rivers State, Nigeria from 2019-2020. Hospital wastewater was taken from different sections at the two University Teaching Hospitals- University of Port Harcourt and Rivers State University Teaching Hospitals. Abattoir effluent water samples were taken at different sites from Trans-Amadi and Rumuodumaya Abattoirs. Chicken cloaca samples as well as Hand swab samples of Butchers were collected at the two Abattoirs. All samples were processed following s...

World Journal of Pharmaceutical and life sciences, Apr 8, 2005

Biomedical Journal of Scientific & Technical Research, 2020

The study was aimed at evaluating the relationship between platelets count, bacterial vaginosis a... more The study was aimed at evaluating the relationship between platelets count, bacterial vaginosis and immune response in cervical human Papillomavirus positive women in Bayelsa State. Cervical smears were collected from apparently healthy subjects attending clinics in Bayelsa State. Consensus primers GP5+/6+ and MY09/11 were used to detect HPV DNA in cervical smears and further subjected to multiplex PCR for molecular typing of HPV subtypes. Nugent method was used for scoring of bacterial vaginoses based on gram staining reaction. Interleukins were studied using sandwich ELISA kits. The prevalence of bacterial vaginosis among HPV positive, HPV negative and high-risk HPV was 35.9% and 18.2% and 51.7% respectively. Bacterial vaginosis expressed statistically significant relationship with high risk HPV (OR=9.64, P=0.002, 95%CI= 1.08-86.26).Interleukin 4 (IL-4), IL-6, IL-8, IL-10, IL-12, IL-17, IL-18, Tumor Necrotic Factor (TNF) and Interferon Gamma (INF-γ) were equally higher in HPV positive participants compared with negative participants and statistically significant. There exists a statistically significantly decrease in platelets count (227.8±57) of HPV positive participants compared with negative participants (259.3±57), (X2=6.8, P=0.01) as well as neutrophils count of 45.2±8.5 of HPV positive compared with negative participants (50.6±8.4), (X 2 =2.9, P=0.01).White blood cell count, monocytes and packed cell volume were equally higher in positive cases compared with negative cases. Bacterial Vaginosis showed a strong relationship with high risk human Papillomavirus, indicating that a healthy vaginal flora will not only protect a woman from the acquisition of sexually transmitted viruses but also high-risk HPV. Interleukins participate in HPV infection and association interferon levels were depleted in HPV infected participants. Thrombocytes and neutrophils are cellular mediators in genital HPV infection. It is recommended therefore that women exposed to high-risk HPV should be screened for bacterial vaginosis, with concomitant estimations of levels of platelets as well as inflammatory cytokines.

Mary Slessor Journal of Medicine, 2009

No Abstract. Mary Slessor Journal of Medicine Vol. 8 (1) 2008: pp. 27-3

South Asian Journal of Research in Microbiology

Aim: The aim of this study was to profile the anti-microbial resistant of Mycobacterial strains c... more Aim: The aim of this study was to profile the anti-microbial resistant of Mycobacterial strains causing cluster pulmonary infections in Bayelsa State, Nigeria. Study Design: Cross sectional facility based. Place and Duration: This study was carried out in all the in tuberculosis centers in all the eight local government area in Bayelsa State, Nigeria between January 2019 to February 2021. Materials and Methods: A total of 102 tuberculosis patients who had been previously diagnosed of Tuberculosis (both new and old cases) participated in the study. Two sputum samples were collected from each subject in a wide mouth screw cape container and falcon tube container respectively. The first was used for GenXpert technique while the second was used for culture on Lowenstein Jensen solid Media. The visible and confirmed Mycobacterium growth was subjected to Line Probe Assay (MTBDRplus assay) and genetic sequencing. The DNA of the isolates where extracted using Genolyse method. The extracted ...

International Journal of Pathogen Research

Aim: To determine the prevalent rates of Escherichia coli O157: H7 and non-O157 strains in of iso... more Aim: To determine the prevalent rates of Escherichia coli O157: H7 and non-O157 strains in of isolates from meat, human stool and abattoir waste water, collected from selected sources in Port Harcourt, Nigeria. Study Design: Case-controlled study. Place and Duration of Study: Selected places in Port Harcourt, Rivers State, Nigeria, between November, 2020 to November, 2021. Methodology: Three hundred and forty -nine (349) samples were analyzed, 80 meat and 63 waste waters from five abattoirs cited in the city, 46 meat samples from five selected sites sold by roadside butchers, 109 patient stool samples and 30 stool samples from food sellers, in addition to 20 stool samples from healthy subjects and 1 commercial bottled water which served as control samples. Tryptone soya broth (TSB) as an enrichment media, selective agar media namely; Eosin methylene blue agar, cefixime tellurite-sorbitol MacConkey agar (CT-SMAC), Chromagar STEC were used to isolate STEC from the samples and serology...

Asian Research Journal of Gynaecology and Obstetrics, Apr 20, 2019

Journal of Complementary and Alternative Medical Research, 2021

Almost every part of a banana plant has use in medicine. Increased bacterial resistance to the co... more Almost every part of a banana plant has use in medicine. Increased bacterial resistance to the conventional antibiotics has led to research into the development of alternative treatments to conventional antibiotics. This study focuses on the antimicrobial properties of banana peels against selected isolates from Urinary Tract Infection sample. The isolates from cultures further analysed with agarose gel electrophoresis for the presence of 16SrRNA and Phylogenetic analysis revealed Staphylococcus sciuri strain, a coagulase‐negative species, Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae and Proteus mirabilis. Fresh unripe and dry unripe were used to determine the antimicrobial activities. A measure of 150grams of both fresh unripe and dry unripe was extracted with 100ml of water solvent (sterile) respectively for about 2days. The solvent extracts were concentrated separately under reduced pressure, 10g of each concentrated solvent extracts were dissolved in 5ml of st...

Asian Journal of Research in Infectious Diseases, 2022

Banana peels refers to the outer coverage of the banana fruit. Apart from serving as a food for m... more Banana peels refers to the outer coverage of the banana fruit. Apart from serving as a food for man and animals, the banana plant also has medicinal values. Urinary Tract Infection is an infection that affects any parts of the urinary system (upper or lower). Its treatment and diagnosis are carried out with urine sample and antibiotics. Increased bacterial resistance to the conventional antibiotics has led to the development of alternative treatment for bacterial disease infections. This study is aimed at assessing the antimicrobial properties of fresh unripe and dried unripe banana peels against selected isolates from Urinary Tract Infection sample. The isolates from culture were further identified using biochemical tests. Fresh unripe and dry unripe were used to determine the antimicrobial activities. A measure of 150 grams of both fresh unripe and dry unripe was extracted with 100ml of 80% Ethanol solvent for about 2 days. The solvent extracts were concentrated separately under r...

Sahel Medical Journal, 2011

... Microbial Profile of wound infections in a Nigerian Teaching Hospital. MBO Ayodele, SD Abbey,... more ... Microbial Profile of wound infections in a Nigerian Teaching Hospital. MBO Ayodele, SD Abbey, EG Nwokah, OK Obunge, GCS Omakwele. Abstract. Background: This study set out to investigate the aerobic and facultative anaerobic ...

International Journal of Pathogen Research

Aim: To assess the Extended Spectrum Beta-Lactamase (ESBL) resistant genes in Salmonella spp isol... more Aim: To assess the Extended Spectrum Beta-Lactamase (ESBL) resistant genes in Salmonella spp isolated from ready-to-eat foods and food handlers in Port Harcourt Metropolis, Nigeria. Study Design: A cross-sectional study. Place and Duration of Study: This study was conducted in selected places in Port Harcourt, between November 2019 and June 2021. Methodology: A total of 350 food specimens and 230 food handlers were collected and the social demographic information were collected using a questionnaire survey. The samples were analysed for contamination with Salmonella species using conventional and molecular methods. Salmonella species were isolated from samples using Salmonella-Shigella agar (SSA), Xylose-lysine desoxycholate agar (XLD), MacConkey agar (MA), Blood agar (BA) after pre-enrichment and enrichment method has been done using peptone water broth and Selenite F. Salmonella Chromogenic medium (SCM) was also used to confirm the isolate. Antibiotic susceptibility patterns of t...

American Journal of Biomedical Sciences

Infections caused by Klebsiella sp. are increasingly becoming difficult to treat partly due to th... more Infections caused by Klebsiella sp. are increasingly becoming difficult to treat partly due to the rise in trend of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella sp. This study aimed to detect the prevalence of ESBL-producing Klebsiella sp. and SHV, TEM, CTX-M, and OXA beta-lactamase encoding genes in isolates of Klebsiella sp. from two healthcare facilities in Port Harcourt. A cross-sectional study of 146 clinical specimens was analyzed using standard bacteriological identification techniques. ESBL-producing Klebsiella sp. was identified using clinical laboratory standard institute standards. Beta-lactamase genes SHV, TEM, OXA, and CTXM were extracted and amplified. Of the 146 clinical specimens; 47 (32.2%) were from males and 99 (67.8%) females. The number of Klebsiella sp. isolated from the female samples 11 (73.3%) was more than 2-fold higher than those isolated from male samples 4 (26.7%). Eighty different bacterial isolates were obtained from which 15 (18.8%) we...

African Journal of Microbiology Research, Apr 14, 2018

The re-emergence and increase in the prevalence of tuberculosis cases and multidrug resistant str... more The re-emergence and increase in the prevalence of tuberculosis cases and multidrug resistant strains is of public health concern. The conventional Acid Fast Bacilli detection tool is weak in the current disease trend especially in children, patients with human immunodeficiency virus (HIV) and low bacterial load. The aim of this research was to assess effectiveness of the Gene Xpert MTB/RIF assay and Microscopy in detectiing Mycobacterium tuberculosis (MTB) in sputum samples in Port Harcourt metropolis. A total of two hundred sputum samples were collected from tuberculosis (TB) suspected patients visiting the antiretroviral and directly observed short treatment clinics at

American Journal of Biomedical Sciences

Journal of Advances in Microbiology

Aim: This study aimed to assess the quality of drinking water in some hydrocarbon-impacted Ogoni ... more Aim: This study aimed to assess the quality of drinking water in some hydrocarbon-impacted Ogoni communities. Study Design: The study employ a cross-sectional and analytical design using stratified sampling method. Place and Duration of Study: Department of Medical Laboratory Science of Rivers State University, Giolee Global Resource Limited and Environmental Consultancy Services between March 2020 and March 2021. Methodology: Water samples were collected from (20 hydrocarbon- impacted communities) in the 4 Local Government Area (LGAs) of Ogoni land. These water samples were analyzed to determine the physicochemical, bacteriological, heavy metal and total petroleum hydrocarbon (TPH) parameters using standard methods and operational procedures. The data obtained were subjected to descriptive statistical analysis. The general linearized model (GIG) was used to generate analysis of variance (ANOVA) mean and standard error and arrange, statistically significant was set as p-value of .05...