Godwin Isitor | RIVERS STATE UNIVERSITY OF SCIENCE AND TECHNOLOGY (original) (raw)
Papers by Godwin Isitor
572-576The extract of K. pinnata was evaluated for its wound healing activity by using excision w... more 572-576The extract of K. pinnata was evaluated for its wound healing activity by using excision wound model in rats. On day 11, animals treated with the ethanolic leaf extract exhibited 86.33 % reduction in the wound area, compared to petroleum jelly treated control (69.36%) and the mupirocin treated standard (85.49%). The hydroxyproline content of extract treated animals was higher, as compared to control and the standard groups. Histological analysis was also consistent with the proposal that K. pinnata leaf extract exhibits significant wound healing potential. The increased rate of wound contraction and hydroxyproline content in the extract treated animals supports the claims made by traditional healers of the benefits obtained from the medicinal use of K. pinnata
American journal of veterinary research, 1979
Journal of Wound Care, 2007
Morinda citrifolia L. is a traditional Polynesian medicinal plant which is apparently useful for ... more Morinda citrifolia L. is a traditional Polynesian medicinal plant which is apparently useful for bowel disorders, skin inflammation, infection, mouth ulcers and wound healing. This study aimed to evaluate the wound-healing activity of Morinda citrifolia fruit juice in streptozotocin-induced diabetic rats. An excision wound model was used. The animals were weight-matched and placed into three groups (n = 6 per group). Group 1 animals served as normal controls, while animals in groups 2 and 3 served as diabetic controls and experimental diabetic animals respectively. All animals were anaesthetised and a full-thickness excision wound (circular area of 300 mm2 and 2 mm deep) was created. Group 3 animals were given the juice of Morinda citrifolia fruit (100 ml per kilogram of body weight) in their drinking water for 10 days. Wound area measurements were taken on days 1, 5 and 11. Blood samples were collected simultaneously for glucose measurement. Granulation tissue that had formed on the wound was excised on day 11 and processed for histological and biochemical analysis. The wound area of the Morinda citrifolia-treated group reduced by 73% (p < 0.001) when compared with the diabetic controls (63%). Significant increases in the weight of granulation tissue (p < 0.001) and hydroxyproline content (p < 0.00 1, 92.16 +/- 4.02) were observed. The protein content was moderately high. Histological studies showed that collagen was laid down faster in the experimental diabetic animals than in the normal control and diabetic control groups. Fasting blood glucose values in the diabetic experimental group had reduced by 29% (p < 0.00 1) compared with the diabetic control animals. There was a good correlation between the wound contraction rate and blood glucose values. This study demonstrates that the juice of Morinda citrifolia fruit significantly reduces blood sugar levels and hastens wound healing in diabetic rats.
Evidence-Based Complementary and Alternative Medicine, 2011
Morinda citrifoliais a medicinal plant used to treat diabetes and liver diseases. The fermented f... more Morinda citrifoliais a medicinal plant used to treat diabetes and liver diseases. The fermented fruit juice of theM. Citrifolia(optical density = 1.25) was used to study the hypoglycemic and hepatoprotective properties in diabetes-induced rats. The rats were randomly distributed into 4 groups (control, diabetic experimental, diabetic standard, and diabetic untreated) of 6 each. Diabetes was induced by administering Streptozotocin (50 mg/kg body weight). Fasting blood glucose, body mass, liver tissue glycogen content, and the extent of liver degeneration were assessed. Diabetic experimental animals were treated withM. citrifoliajuice (2 ml/kg, twice a day) and diabetic standard with reference hypoglycemic drug, glibenclamide orally for 20 days. Both the groups exhibited a significant reduction in blood glucose level of 150 mg/dl and 125 mg/dl , respectively, as compared to diabetic untreated with FBS = 360.0 mg/dl , (). On 10th day of experiment, diabetic experimental animals exhibit...
Copyright © 2011 B. Shivananda Nayak et al. This is an open access article distributed under the ... more Copyright © 2011 B. Shivananda Nayak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Morinda citrifolia is a medicinal plant used to treat diabetes and liver diseases. The fermented fruit juice of the M. Citrifolia (optical density = 1.25) was used to study the hypoglycemic and hepatoprotective properties in diabetes-induced rats. The rats were randomly distributed into 4 groups (control, diabetic experimental, diabetic standard, and diabetic untreated) of 6 each. Diabetes was induced by administering Streptozotocin (50 mg/kg body weight). Fasting blood glucose, body mass, liver tissue glycogen content, and the extent of liver degeneration were assessed. Diabetic experimental animals were treated with M. citrifolia juice (2 ml/kg, twice a day) and diabetic standard with reference hypoglycemic drug, glibenclamide...
An incidence of canine circumanal gland adenoma (CGA) is reported, apparently for the first time,... more An incidence of canine circumanal gland adenoma (CGA) is reported, apparently for the first time, in the island of Trinidad and Tobago. The case involved an ll-year-old Rottweiler dog which was presented in relatively good health except for noticeable masses around the perianal region. Apart from routine histopathological investigation which enabled a definitive diagnosis of CGA with secondary vascularization, analysis of the nuclear chromatin texture was performed on the digitalized images of both reserve and hepatoid cells of the neoplasm. The mean nuclear pixel intensity value of the images of processed CGA reserve cells was significantly lower than those of the processed CGA hepatoid cells (P<O.I). Our observation based on the texture analytical data suggests a high degree of rate of multiplication of the reserve cells at the expense of degenerating hepatoid cells. The relevance of this finding is discussed within the context of vascular supply to the neoplastic cells.
The study was done to document and compare the cytoarchitectural details of the cochlear nuclear ... more The study was done to document and compare the cytoarchitectural details of the cochlear nuclear complex (CNC) in two species of bats with different forage strategies, Carollia perspicillata (fruit eating) and Phyllostomus hastatus (carnivorous/omnivorous). The CNC was larger and the subdivisions were distinct in P.hastatus than the C.perspillata. The CNC of both bats comprised of two main divisions: dorsal cochlear nucleus (DCN) and ventral cochlear nucleus (VCN). The DCN of both bats was further subdivided into two subdivisions: dorsomedial and ventromedial whereas the VCN was subdivided into three: anteroventral (AVCN), posteroventral (PVCN) and a ventrolateral (LVCN). The main cell type of the DCN was small round cells measuring approximately 5 µm in diameter in both bats. The AVCN was principally made up of large multipolar cells (approximately 17 µm in diameter in C.perspicillata and 17-32 µm diameters in P.hastatus).The PVCN comprised of predominantly ovals cells measuring between 7.5 and 10 µm in diameter in both the bats. The LVCN comprised of small round cells with 5 µm in diameter in both the bats. The larger CNC with distinct subdivisions and the larger sized multipolar cells in the AVCN of the P.hastatus suggest that they depend mainly on echolocation for feeding while the C.perspillata, a fruit eating bat that uses echolocation to a lesser extent.
Indian Journal of Experimental Biology, Jun 1, 2010
The extract of K. pinnata was evaluated for its wound healing activity by using excision wound mo... more The extract of K. pinnata was evaluated for its wound healing activity by using excision wound model in rats. On day 11, animals treated with the ethanolic leaf extract exhibited 86.33% reduction in the wound area, compared to petroleum jelly treated control (69.36%) and the mupirocin treated standard (85.49%). The hydroxyproline content of extract treated animals was higher, as compared to control and the standard groups. Histological analysis was also consistent with the proposal that K. pinnata leaf extract exhibits significant wound healing potential. The increased rate of wound contraction and hydroxyproline content in the extract treated animals supports the claims made by traditional healers of the benefits obtained from the medicinal use of K. pinnata.
Phytotherapy Research, 2011
Vitis vinifera (grape) and Vaccinium macrocarpon (cranberry) are well known medicinal plants; mos... more Vitis vinifera (grape) and Vaccinium macrocarpon (cranberry) are well known medicinal plants; most of the pharmacologically active phytochemicals have been isolated from the skin, fruit juice, fermented extract and alcohol fractions of the plants above. Here, the pharmacological properties of the phytochemical constituents present in oils of cranberry and grape were investigated. The oil of grape and cranberry has been evaluated for their wound healing activity by using an excision wound model in rats. The animals were divided into four groups of six each (n = 6). The experimental group 1 and 2 animals were treated topically with the grape and cranberry oil (100 mg/kg body weight), respectively. The controls were treated with petroleum jelly. The standard group of animals were treated with mupirocin ointment (100 mg/kg body weight). The healing was assessed by the rate of wound contraction and hydroxyproline content. On day 13, animals treated with cranberry oil exhibited a (88.1%) reduction in the wound area compared with grape-oil treated (84.6%), controls (74.1%) and standard group animals (78.4%) (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001). The hydroxyproline content of the granulation tissue was significantly higher in the animals treated with cranberry and the grape-oil (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.000). Comparative investigation of the curative properties of the oils of V. vinifera and V. macrocarpon revealed a significant result which suggests their wound-healing potential.
American Journal of Veterinary Research
American Journal of Veterinary Research
Intranuclear cisternal and vesicular structures, bound by a single membrane, were observed for th... more Intranuclear cisternal and vesicular structures, bound by a single membrane, were observed for the first time in some glandular "bird's eye" cells of canine circummanal gland carcinomas. The role of these intranuclear structures is not clear. It is suggested that they may be involved in cellular adaptation to an environment of nutrient deficiency, as a result of cell overcrowding.
572-576The extract of K. pinnata was evaluated for its wound healing activity by using excision w... more 572-576The extract of K. pinnata was evaluated for its wound healing activity by using excision wound model in rats. On day 11, animals treated with the ethanolic leaf extract exhibited 86.33 % reduction in the wound area, compared to petroleum jelly treated control (69.36%) and the mupirocin treated standard (85.49%). The hydroxyproline content of extract treated animals was higher, as compared to control and the standard groups. Histological analysis was also consistent with the proposal that K. pinnata leaf extract exhibits significant wound healing potential. The increased rate of wound contraction and hydroxyproline content in the extract treated animals supports the claims made by traditional healers of the benefits obtained from the medicinal use of K. pinnata
American journal of veterinary research, 1979
Journal of Wound Care, 2007
Morinda citrifolia L. is a traditional Polynesian medicinal plant which is apparently useful for ... more Morinda citrifolia L. is a traditional Polynesian medicinal plant which is apparently useful for bowel disorders, skin inflammation, infection, mouth ulcers and wound healing. This study aimed to evaluate the wound-healing activity of Morinda citrifolia fruit juice in streptozotocin-induced diabetic rats. An excision wound model was used. The animals were weight-matched and placed into three groups (n = 6 per group). Group 1 animals served as normal controls, while animals in groups 2 and 3 served as diabetic controls and experimental diabetic animals respectively. All animals were anaesthetised and a full-thickness excision wound (circular area of 300 mm2 and 2 mm deep) was created. Group 3 animals were given the juice of Morinda citrifolia fruit (100 ml per kilogram of body weight) in their drinking water for 10 days. Wound area measurements were taken on days 1, 5 and 11. Blood samples were collected simultaneously for glucose measurement. Granulation tissue that had formed on the wound was excised on day 11 and processed for histological and biochemical analysis. The wound area of the Morinda citrifolia-treated group reduced by 73% (p < 0.001) when compared with the diabetic controls (63%). Significant increases in the weight of granulation tissue (p < 0.001) and hydroxyproline content (p < 0.00 1, 92.16 +/- 4.02) were observed. The protein content was moderately high. Histological studies showed that collagen was laid down faster in the experimental diabetic animals than in the normal control and diabetic control groups. Fasting blood glucose values in the diabetic experimental group had reduced by 29% (p < 0.00 1) compared with the diabetic control animals. There was a good correlation between the wound contraction rate and blood glucose values. This study demonstrates that the juice of Morinda citrifolia fruit significantly reduces blood sugar levels and hastens wound healing in diabetic rats.
Evidence-Based Complementary and Alternative Medicine, 2011
Morinda citrifoliais a medicinal plant used to treat diabetes and liver diseases. The fermented f... more Morinda citrifoliais a medicinal plant used to treat diabetes and liver diseases. The fermented fruit juice of theM. Citrifolia(optical density = 1.25) was used to study the hypoglycemic and hepatoprotective properties in diabetes-induced rats. The rats were randomly distributed into 4 groups (control, diabetic experimental, diabetic standard, and diabetic untreated) of 6 each. Diabetes was induced by administering Streptozotocin (50 mg/kg body weight). Fasting blood glucose, body mass, liver tissue glycogen content, and the extent of liver degeneration were assessed. Diabetic experimental animals were treated withM. citrifoliajuice (2 ml/kg, twice a day) and diabetic standard with reference hypoglycemic drug, glibenclamide orally for 20 days. Both the groups exhibited a significant reduction in blood glucose level of 150 mg/dl and 125 mg/dl , respectively, as compared to diabetic untreated with FBS = 360.0 mg/dl , (). On 10th day of experiment, diabetic experimental animals exhibit...
Copyright © 2011 B. Shivananda Nayak et al. This is an open access article distributed under the ... more Copyright © 2011 B. Shivananda Nayak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Morinda citrifolia is a medicinal plant used to treat diabetes and liver diseases. The fermented fruit juice of the M. Citrifolia (optical density = 1.25) was used to study the hypoglycemic and hepatoprotective properties in diabetes-induced rats. The rats were randomly distributed into 4 groups (control, diabetic experimental, diabetic standard, and diabetic untreated) of 6 each. Diabetes was induced by administering Streptozotocin (50 mg/kg body weight). Fasting blood glucose, body mass, liver tissue glycogen content, and the extent of liver degeneration were assessed. Diabetic experimental animals were treated with M. citrifolia juice (2 ml/kg, twice a day) and diabetic standard with reference hypoglycemic drug, glibenclamide...
An incidence of canine circumanal gland adenoma (CGA) is reported, apparently for the first time,... more An incidence of canine circumanal gland adenoma (CGA) is reported, apparently for the first time, in the island of Trinidad and Tobago. The case involved an ll-year-old Rottweiler dog which was presented in relatively good health except for noticeable masses around the perianal region. Apart from routine histopathological investigation which enabled a definitive diagnosis of CGA with secondary vascularization, analysis of the nuclear chromatin texture was performed on the digitalized images of both reserve and hepatoid cells of the neoplasm. The mean nuclear pixel intensity value of the images of processed CGA reserve cells was significantly lower than those of the processed CGA hepatoid cells (P<O.I). Our observation based on the texture analytical data suggests a high degree of rate of multiplication of the reserve cells at the expense of degenerating hepatoid cells. The relevance of this finding is discussed within the context of vascular supply to the neoplastic cells.
The study was done to document and compare the cytoarchitectural details of the cochlear nuclear ... more The study was done to document and compare the cytoarchitectural details of the cochlear nuclear complex (CNC) in two species of bats with different forage strategies, Carollia perspicillata (fruit eating) and Phyllostomus hastatus (carnivorous/omnivorous). The CNC was larger and the subdivisions were distinct in P.hastatus than the C.perspillata. The CNC of both bats comprised of two main divisions: dorsal cochlear nucleus (DCN) and ventral cochlear nucleus (VCN). The DCN of both bats was further subdivided into two subdivisions: dorsomedial and ventromedial whereas the VCN was subdivided into three: anteroventral (AVCN), posteroventral (PVCN) and a ventrolateral (LVCN). The main cell type of the DCN was small round cells measuring approximately 5 µm in diameter in both bats. The AVCN was principally made up of large multipolar cells (approximately 17 µm in diameter in C.perspicillata and 17-32 µm diameters in P.hastatus).The PVCN comprised of predominantly ovals cells measuring between 7.5 and 10 µm in diameter in both the bats. The LVCN comprised of small round cells with 5 µm in diameter in both the bats. The larger CNC with distinct subdivisions and the larger sized multipolar cells in the AVCN of the P.hastatus suggest that they depend mainly on echolocation for feeding while the C.perspillata, a fruit eating bat that uses echolocation to a lesser extent.
Indian Journal of Experimental Biology, Jun 1, 2010
The extract of K. pinnata was evaluated for its wound healing activity by using excision wound mo... more The extract of K. pinnata was evaluated for its wound healing activity by using excision wound model in rats. On day 11, animals treated with the ethanolic leaf extract exhibited 86.33% reduction in the wound area, compared to petroleum jelly treated control (69.36%) and the mupirocin treated standard (85.49%). The hydroxyproline content of extract treated animals was higher, as compared to control and the standard groups. Histological analysis was also consistent with the proposal that K. pinnata leaf extract exhibits significant wound healing potential. The increased rate of wound contraction and hydroxyproline content in the extract treated animals supports the claims made by traditional healers of the benefits obtained from the medicinal use of K. pinnata.
Phytotherapy Research, 2011
Vitis vinifera (grape) and Vaccinium macrocarpon (cranberry) are well known medicinal plants; mos... more Vitis vinifera (grape) and Vaccinium macrocarpon (cranberry) are well known medicinal plants; most of the pharmacologically active phytochemicals have been isolated from the skin, fruit juice, fermented extract and alcohol fractions of the plants above. Here, the pharmacological properties of the phytochemical constituents present in oils of cranberry and grape were investigated. The oil of grape and cranberry has been evaluated for their wound healing activity by using an excision wound model in rats. The animals were divided into four groups of six each (n = 6). The experimental group 1 and 2 animals were treated topically with the grape and cranberry oil (100 mg/kg body weight), respectively. The controls were treated with petroleum jelly. The standard group of animals were treated with mupirocin ointment (100 mg/kg body weight). The healing was assessed by the rate of wound contraction and hydroxyproline content. On day 13, animals treated with cranberry oil exhibited a (88.1%) reduction in the wound area compared with grape-oil treated (84.6%), controls (74.1%) and standard group animals (78.4%) (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001). The hydroxyproline content of the granulation tissue was significantly higher in the animals treated with cranberry and the grape-oil (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.000). Comparative investigation of the curative properties of the oils of V. vinifera and V. macrocarpon revealed a significant result which suggests their wound-healing potential.
American Journal of Veterinary Research
American Journal of Veterinary Research
Intranuclear cisternal and vesicular structures, bound by a single membrane, were observed for th... more Intranuclear cisternal and vesicular structures, bound by a single membrane, were observed for the first time in some glandular "bird's eye" cells of canine circummanal gland carcinomas. The role of these intranuclear structures is not clear. It is suggested that they may be involved in cellular adaptation to an environment of nutrient deficiency, as a result of cell overcrowding.