Fatih Seyis | Recep Tayyip Erdogan University (original) (raw)
Papers by Fatih Seyis
Geliş Tarihi : 21.07.2006 ÖZET: Bazen yakın akraba formlarda bulunan bir genin kültür bitkilerine... more Geliş Tarihi : 21.07.2006 ÖZET: Bazen yakın akraba formlarda bulunan bir genin kültür bitkilerine aktarılması faydalı olabilmektedir. Bu işlemdeki amaç büyümenin güçlendirilmesi veya hastalıklara dayanıklılığın aktarılması olabilmektedir. Bu amaçla yapılacak olan melezlemelerde uyuşmazlık ortaya çıkabilmekte ve normal yaşama kabiliyetine sahip tohum gelişmeyebilmektedir. Bununla birlikte embriyo oluşabilir ve tohum gelişme safhasının mümkün olduğunca erken döneminde alınan bu embriyo besi ortamına aktarılarak kurtarılmakta ve gelişmesi sağlanmaktadır. Yapılan bu işlem ile embriyodan kendiliğinden bitkicik oluşumunun sağlanması, tohum gelişmesi ve çimlenmesindeki olumsuzlukları ortadan kaldırılması hedeflenmektedir. Uzak akraba formlar arasında yapılan melezlemeler sonucu oluşan zigot embriyoya dönüşmeden kallus meydana getirebilmekte ve bu kallustan bitkicikler gelişmektedir. Bu makalede, türler arası melezlemelerde ortaya çıkan uyuşmazlık problemini aşmada kullanılan embriyo kültürü üzerinde durulacaktır.
Plant Breeding, 2007
Resynthesized (RS) forms of rapeseed (Brassica napus L.; genome AACC, 2n = 38) generated from int... more Resynthesized (RS) forms of rapeseed (Brassica napus L.; genome AACC, 2n = 38) generated from interspecific hybridization between suitable genotypes of its diploid progenitors Brassica rapa L. (syn. campestris; genome AA, 2n = 20) and Brassica oleracea L. (CC, 2n = 18) represent a potentially useful resource to introduce resistance against the fungal pathogen Verticillium longisporum into the gene pool of oilseed rape. Numerous cabbage (B. oleracea) accessions are known with resistance to V. longisporum; however, B. oleracea generally has high levels of erucic acid and glucosinolates in the seed, which reduces the suitability of resulting RS rapeseed lines for oilseed rape breeding. In this study resistance against V. longisporum was identified in the cabbage accession Kashirka 202 (B. oleracea convar. capitata), a zero erucic acid mutant, and RS rapeseed lines were generated by crossing the resistant genotype with two spring turnip rape accessions (B. rapa ssp. olerifera) with zero erucic acid. One of the resulting zero erucic acid RS rapeseed lines was found to have a high level of resistance to V. longisporum compared with both parental accessions and with B. napus controls. A number of other zero erucic acid RS lines showed resistance levels comparable to the parental accessions. In the most resistant RS lines the resistance and zero erucic acid traits were combined with variable seed glucosinolate contents. Erucic acid-free RS rapeseed with moderate seed glucosinolate content represents an ideal basic material for introgression of quantitative V. longisporum resistance derived from B. oleracea and B. rapa into elite oilseed rape breeding lines.
Plant Breeding, 2003
Resynthesized (RS) rapeseed generated from interspecific hybridization between suitable forms of ... more Resynthesized (RS) rapeseed generated from interspecific hybridization between suitable forms of Brassica rapa L. (syn. campestris; genome AA, 2n ¼ 20) and B. oleracea L. (CC, 2n ¼ 18) represents a potentially important resource to expand genetic diversity in the narrow gene pool of oilseed rape (B. napus L., AACC, 2n ¼ 38). In this study 165 RS rapeseed lines originating from crosses between an Indian Yellow Sarson (B. rapa ssp. trilocularis) and five different cauliflower (B. oleracea convar. botrytis) cultivars were studied using amplified fragment length polymorphism (AFLP) markers and their genetic diversity was compared in relationship to an assortment of 40 diverse spring oilseed and fodder rape varieties. Using three AFLP primer combinations, a total of 467 polymorphic bands were scored. Cluster analysis allowed differentiation among the different RS lines, which, as expected, were genetically highly divergent from the cultivars. The genetic diversity of the material is discussed in relation to its morphological variability with a view to the implementation of RS lines in oilseed rape breeding.
Plant Breeding, 2007
Resynthesized (RS) forms of rapeseed (Brassica napus L.; genome AACC, 2n = 38) generated from int... more Resynthesized (RS) forms of rapeseed (Brassica napus L.; genome AACC, 2n = 38) generated from interspecific hybridization between suitable genotypes of its diploid progenitors Brassica rapa L. (syn. campestris; genome AA, 2n = 20) and Brassica oleracea L. (CC, 2n = 18) represent a potentially useful resource to introduce resistance against the fungal pathogen Verticillium longisporum into the gene pool of oilseed rape. Numerous cabbage (B. oleracea) accessions are known with resistance to V. longisporum; however, B. oleracea generally has high levels of erucic acid and glucosinolates in the seed, which reduces the suitability of resulting RS rapeseed lines for oilseed rape breeding. In this study resistance against V. longisporum was identified in the cabbage accession Kashirka 202 (B. oleracea convar. capitata), a zero erucic acid mutant, and RS rapeseed lines were generated by crossing the resistant genotype with two spring turnip rape accessions (B. rapa ssp. olerifera) with zero erucic acid. One of the resulting zero erucic acid RS rapeseed lines was found to have a high level of resistance to V. longisporum compared with both parental accessions and with B. napus controls. A number of other zero erucic acid RS lines showed resistance levels comparable to the parental accessions. In the most resistant RS lines the resistance and zero erucic acid traits were combined with variable seed glucosinolate contents. Erucic acid-free RS rapeseed with moderate seed glucosinolate content represents an ideal basic material for introgression of quantitative V. longisporum resistance derived from B. oleracea and B. rapa into elite oilseed rape breeding lines.
Plant Breeding, 2003
Resynthesized (RS) rapeseed generated from interspecific hybridization between suitable forms of ... more Resynthesized (RS) rapeseed generated from interspecific hybridization between suitable forms of Brassica rapa L. (syn. campestris; genome AA, 2n ¼ 20) and B. oleracea L. (CC, 2n ¼ 18) represents a potentially important resource to expand genetic diversity in the narrow gene pool of oilseed rape (B. napus L., AACC, 2n ¼ 38). In this study 165 RS rapeseed lines originating from crosses between an Indian Yellow Sarson (B. rapa ssp. trilocularis) and five different cauliflower (B. oleracea convar. botrytis) cultivars were studied using amplified fragment length polymorphism (AFLP) markers and their genetic diversity was compared in relationship to an assortment of 40 diverse spring oilseed and fodder rape varieties. Using three AFLP primer combinations, a total of 467 polymorphic bands were scored. Cluster analysis allowed differentiation among the different RS lines, which, as expected, were genetically highly divergent from the cultivars. The genetic diversity of the material is discussed in relation to its morphological variability with a view to the implementation of RS lines in oilseed rape breeding.
Pharmaceutical Biology, 2010
Geliş Tarihi : 21.07.2006 ÖZET: Bazen yakın akraba formlarda bulunan bir genin kültür bitkilerine... more Geliş Tarihi : 21.07.2006 ÖZET: Bazen yakın akraba formlarda bulunan bir genin kültür bitkilerine aktarılması faydalı olabilmektedir. Bu işlemdeki amaç büyümenin güçlendirilmesi veya hastalıklara dayanıklılığın aktarılması olabilmektedir. Bu amaçla yapılacak olan melezlemelerde uyuşmazlık ortaya çıkabilmekte ve normal yaşama kabiliyetine sahip tohum gelişmeyebilmektedir. Bununla birlikte embriyo oluşabilir ve tohum gelişme safhasının mümkün olduğunca erken döneminde alınan bu embriyo besi ortamına aktarılarak kurtarılmakta ve gelişmesi sağlanmaktadır. Yapılan bu işlem ile embriyodan kendiliğinden bitkicik oluşumunun sağlanması, tohum gelişmesi ve çimlenmesindeki olumsuzlukları ortadan kaldırılması hedeflenmektedir. Uzak akraba formlar arasında yapılan melezlemeler sonucu oluşan zigot embriyoya dönüşmeden kallus meydana getirebilmekte ve bu kallustan bitkicikler gelişmektedir. Bu makalede, türler arası melezlemelerde ortaya çıkan uyuşmazlık problemini aşmada kullanılan embriyo kültürü üzerinde durulacaktır.
Plant Breeding, 2007
Resynthesized (RS) forms of rapeseed (Brassica napus L.; genome AACC, 2n = 38) generated from int... more Resynthesized (RS) forms of rapeseed (Brassica napus L.; genome AACC, 2n = 38) generated from interspecific hybridization between suitable genotypes of its diploid progenitors Brassica rapa L. (syn. campestris; genome AA, 2n = 20) and Brassica oleracea L. (CC, 2n = 18) represent a potentially useful resource to introduce resistance against the fungal pathogen Verticillium longisporum into the gene pool of oilseed rape. Numerous cabbage (B. oleracea) accessions are known with resistance to V. longisporum; however, B. oleracea generally has high levels of erucic acid and glucosinolates in the seed, which reduces the suitability of resulting RS rapeseed lines for oilseed rape breeding. In this study resistance against V. longisporum was identified in the cabbage accession Kashirka 202 (B. oleracea convar. capitata), a zero erucic acid mutant, and RS rapeseed lines were generated by crossing the resistant genotype with two spring turnip rape accessions (B. rapa ssp. olerifera) with zero erucic acid. One of the resulting zero erucic acid RS rapeseed lines was found to have a high level of resistance to V. longisporum compared with both parental accessions and with B. napus controls. A number of other zero erucic acid RS lines showed resistance levels comparable to the parental accessions. In the most resistant RS lines the resistance and zero erucic acid traits were combined with variable seed glucosinolate contents. Erucic acid-free RS rapeseed with moderate seed glucosinolate content represents an ideal basic material for introgression of quantitative V. longisporum resistance derived from B. oleracea and B. rapa into elite oilseed rape breeding lines.
Plant Breeding, 2003
Resynthesized (RS) rapeseed generated from interspecific hybridization between suitable forms of ... more Resynthesized (RS) rapeseed generated from interspecific hybridization between suitable forms of Brassica rapa L. (syn. campestris; genome AA, 2n ¼ 20) and B. oleracea L. (CC, 2n ¼ 18) represents a potentially important resource to expand genetic diversity in the narrow gene pool of oilseed rape (B. napus L., AACC, 2n ¼ 38). In this study 165 RS rapeseed lines originating from crosses between an Indian Yellow Sarson (B. rapa ssp. trilocularis) and five different cauliflower (B. oleracea convar. botrytis) cultivars were studied using amplified fragment length polymorphism (AFLP) markers and their genetic diversity was compared in relationship to an assortment of 40 diverse spring oilseed and fodder rape varieties. Using three AFLP primer combinations, a total of 467 polymorphic bands were scored. Cluster analysis allowed differentiation among the different RS lines, which, as expected, were genetically highly divergent from the cultivars. The genetic diversity of the material is discussed in relation to its morphological variability with a view to the implementation of RS lines in oilseed rape breeding.
Plant Breeding, 2007
Resynthesized (RS) forms of rapeseed (Brassica napus L.; genome AACC, 2n = 38) generated from int... more Resynthesized (RS) forms of rapeseed (Brassica napus L.; genome AACC, 2n = 38) generated from interspecific hybridization between suitable genotypes of its diploid progenitors Brassica rapa L. (syn. campestris; genome AA, 2n = 20) and Brassica oleracea L. (CC, 2n = 18) represent a potentially useful resource to introduce resistance against the fungal pathogen Verticillium longisporum into the gene pool of oilseed rape. Numerous cabbage (B. oleracea) accessions are known with resistance to V. longisporum; however, B. oleracea generally has high levels of erucic acid and glucosinolates in the seed, which reduces the suitability of resulting RS rapeseed lines for oilseed rape breeding. In this study resistance against V. longisporum was identified in the cabbage accession Kashirka 202 (B. oleracea convar. capitata), a zero erucic acid mutant, and RS rapeseed lines were generated by crossing the resistant genotype with two spring turnip rape accessions (B. rapa ssp. olerifera) with zero erucic acid. One of the resulting zero erucic acid RS rapeseed lines was found to have a high level of resistance to V. longisporum compared with both parental accessions and with B. napus controls. A number of other zero erucic acid RS lines showed resistance levels comparable to the parental accessions. In the most resistant RS lines the resistance and zero erucic acid traits were combined with variable seed glucosinolate contents. Erucic acid-free RS rapeseed with moderate seed glucosinolate content represents an ideal basic material for introgression of quantitative V. longisporum resistance derived from B. oleracea and B. rapa into elite oilseed rape breeding lines.
Plant Breeding, 2003
Resynthesized (RS) rapeseed generated from interspecific hybridization between suitable forms of ... more Resynthesized (RS) rapeseed generated from interspecific hybridization between suitable forms of Brassica rapa L. (syn. campestris; genome AA, 2n ¼ 20) and B. oleracea L. (CC, 2n ¼ 18) represents a potentially important resource to expand genetic diversity in the narrow gene pool of oilseed rape (B. napus L., AACC, 2n ¼ 38). In this study 165 RS rapeseed lines originating from crosses between an Indian Yellow Sarson (B. rapa ssp. trilocularis) and five different cauliflower (B. oleracea convar. botrytis) cultivars were studied using amplified fragment length polymorphism (AFLP) markers and their genetic diversity was compared in relationship to an assortment of 40 diverse spring oilseed and fodder rape varieties. Using three AFLP primer combinations, a total of 467 polymorphic bands were scored. Cluster analysis allowed differentiation among the different RS lines, which, as expected, were genetically highly divergent from the cultivars. The genetic diversity of the material is discussed in relation to its morphological variability with a view to the implementation of RS lines in oilseed rape breeding.
Pharmaceutical Biology, 2010