Leyla Tskhovrebova | People's Friendship University of Russia (original) (raw)
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Papers by Leyla Tskhovrebova
Head and Neck Tumors (HNT)
Introduction. Head and neck cancer is a heterogenous group of malignant tumors of different etiol... more Introduction. Head and neck cancer is a heterogenous group of malignant tumors of different etiologies, molecular mechanisms of which are still insufficiently studied.Aim. Investigation of DNA methylation status of some tumor associated genes (RASSF1A, RASSF2, RASSF5, CDO1, MEST and WIF1) in patients with head and neck squamous cell carcinoma.Materials and methods. The DNA methylation level of normal and tumor tissues was analyzed using bisulfite conversion and methylation-sensitive high-resolution melting in 25 patients (21 men and 4 women) diagnosed with neck squamous cell carcinoma.Results. There were significant differences in levels of DNA methylation between tumor and normal tissues in the CDO1 and WIF1 genes in all groups and subgroups of patients (larynx and other cancers, squamous cell carcinoma keratinizing and non-keratinizing, primary and recurrent tumor, smokers and non-smokers). The methylation level in the CDO1 gene in tumor tissue was significantly increased in the T...
Head and neck squamous cell carcinoma (HNSCC) takes the sixth place among the most common cancers... more Head and neck squamous cell carcinoma (HNSCC) takes the sixth place among the most common cancers in the world. Abnormal methylation can be one of the reasons for this cancer. The aim of this study was to investigate the DNA promotor methylation status of cancer-associated genes (ATM, APC, CDO1, RB1, TP53, WIF1) in patients with HNSCC. Bisulfite Conversion and Methylation-Sensitive High-Resolution Melting was used for analysis of the DNA methylation level of normal and tumor tissues in 44 patients. There were significant differences in DNA methylation level between patient’s tumor and normal tissues for CDO1 and WIF1 genes in all subjects and subgroups (p<0.05). In T3 subgroup there was significant correlation between CDO1 gene methylation and age in the normal tissue. The same correlation was detected also for the WIF1 gene methylation in tumor tissue samples in the subgroup with T3 and in normal tissue samples in the subgroup with T4 (p<0,05). In all genes no significant dif...
Aging, 2011
Genome stability of human embryonic stem cells (hESC) is an important issue because even minor ge... more Genome stability of human embryonic stem cells (hESC) is an important issue because even minor genetic alterations can negatively impact cell functionality and safety. The incorrect repair of DNA double-stranded breaks (DSBs) is the ultimate cause of the formation of chromosomal aberrations. Using G2 radiosensitivity assay, we analyzed chromosomal aberrations in pluripotent stem cells and somatic cells. The chromatid exchange aberration rates in hESCs increased manifold 2 hours after irradiation as compared with their differentiated derivatives, but the frequency of radiation-induced chromatid breaks was similar. The rate of radiation-induced chromatid exchanges in hESCs and differentiated cells exhibited a quadratic dose response, revealing two-hit mechanism of exchange formation suggesting that a non-homologous end joining (NHEJ) repair may contribute to their formation. Inhibition of DNA-PK, a key NHEJ component, by NU7026 resulted in a significant decrease in radiation-induced c...
Life, 2022
Background: In recent years, the interest in genetic predisposition studies for coronary artery d... more Background: In recent years, the interest in genetic predisposition studies for coronary artery disease and restenosis has increased. Studies show that polymorphisms of genes encoding folate cycle and homocysteine metabolism enzymes significantly contribute to atherogenesis and endothelial dysfunction. The purpose of this study was to examine some SNPs of genes coding for folate cycle enzymes and DNA methyltransferases as risk factors for in-stent restenosis. Methods: The study included 113 patients after stent implantation and 62 patients without signs of coronary artery disease at coronary angiography as the control group. Real-time PCR and RFLP-PCR were applied to genotype all participants for MTHFR rs1801133, MTHFR rs1801131, MTR rs1805087, MTRR rs1801394, DNMT1 rs8101626, DNMT3B rs1569686, and DNMT3B rs2424913 gene polymorphisms. Statistical data processing was carried out using the R language and the SPSS Statistics 20 software. Results: Statistically significant differences i...
![Research paper thumbnail of [Gene polymorphisms of renin-angiotensin-aldosterone system components and the progression of chronic kidney diseases]](https://attachments.academia-assets.com/75989384/thumbnails/1.jpg)
](Postȩpy higieny i medycyny doświadczalnej (Online), Jan 30, 2010
The renin-angiotensin-aldosterone system (RAAS) plays an important role in the pathogenesis of hy... more The renin-angiotensin-aldosterone system (RAAS) plays an important role in the pathogenesis of hypertension as well as cardiovascular diseases and chronic kidney diseases. Among the most frequently studied RAAS gene polymorphisms are the angiotensin-converting enzyme insertion/deletion (I/D), angiotensinogen M235T and angiotensin II receptor type 1 A1166C polymorphisms. A significant correlation was found between the I/D polymorphism and cardiovascular morbidity and mortality rates. However, there was no significant correlation between I/D, M235T, A1166C polymorphism and arterial hypertension. The role of I/D polymorphism in the development and progression of chronic kidney disease is also non-conclusive. However, DD genotype has been identified as relevant for loss of renal function both in patients with IgA nephropathy and in patients of Asian origin with diabetic nephropathy. The relationship between RAAS gene polymorphism and transplanted kidney function has not been confirmed i...
2 Институт общей генетики им. Н.И.Вавилова, РАН, Москва, Россия. В данной работе исследовали влия... more 2 Институт общей генетики им. Н.И.Вавилова, РАН, Москва, Россия. В данной работе исследовали влияние препарата Кантепарин на цитотокси ческое действие спиртового экстракта болиголова (Сonium maculatum из се мейства зонтичные Umbelliferal) на лимфоцитах человека.Было обнаружено защитное действие препарата Кантепарин, которое проявляется как в сниже нии общего количества гибнущих клеток, так и в уменьшении доли некрозов и, соответственно, увеличении доли апоптозов в спектре индуцированной ги бели клеток.Факт снижения доли некрозов наиболее важен в случае возмож ного применения болиголова или других химиопрепаратов в сочетании с пре паратом Кантепарин в качестве противоопухолевых агентов. Ключевые слова: препарат Кантепарин, цитотоксическое действие, экстракт болиголова, лимфоциты человека.
Head and Neck Tumors (HNT)
Introduction. Head and neck cancer is a heterogenous group of malignant tumors of different etiol... more Introduction. Head and neck cancer is a heterogenous group of malignant tumors of different etiologies, molecular mechanisms of which are still insufficiently studied.Aim. Investigation of DNA methylation status of some tumor associated genes (RASSF1A, RASSF2, RASSF5, CDO1, MEST and WIF1) in patients with head and neck squamous cell carcinoma.Materials and methods. The DNA methylation level of normal and tumor tissues was analyzed using bisulfite conversion and methylation-sensitive high-resolution melting in 25 patients (21 men and 4 women) diagnosed with neck squamous cell carcinoma.Results. There were significant differences in levels of DNA methylation between tumor and normal tissues in the CDO1 and WIF1 genes in all groups and subgroups of patients (larynx and other cancers, squamous cell carcinoma keratinizing and non-keratinizing, primary and recurrent tumor, smokers and non-smokers). The methylation level in the CDO1 gene in tumor tissue was significantly increased in the T...
Head and neck squamous cell carcinoma (HNSCC) takes the sixth place among the most common cancers... more Head and neck squamous cell carcinoma (HNSCC) takes the sixth place among the most common cancers in the world. Abnormal methylation can be one of the reasons for this cancer. The aim of this study was to investigate the DNA promotor methylation status of cancer-associated genes (ATM, APC, CDO1, RB1, TP53, WIF1) in patients with HNSCC. Bisulfite Conversion and Methylation-Sensitive High-Resolution Melting was used for analysis of the DNA methylation level of normal and tumor tissues in 44 patients. There were significant differences in DNA methylation level between patient’s tumor and normal tissues for CDO1 and WIF1 genes in all subjects and subgroups (p<0.05). In T3 subgroup there was significant correlation between CDO1 gene methylation and age in the normal tissue. The same correlation was detected also for the WIF1 gene methylation in tumor tissue samples in the subgroup with T3 and in normal tissue samples in the subgroup with T4 (p<0,05). In all genes no significant dif...
Aging, 2011
Genome stability of human embryonic stem cells (hESC) is an important issue because even minor ge... more Genome stability of human embryonic stem cells (hESC) is an important issue because even minor genetic alterations can negatively impact cell functionality and safety. The incorrect repair of DNA double-stranded breaks (DSBs) is the ultimate cause of the formation of chromosomal aberrations. Using G2 radiosensitivity assay, we analyzed chromosomal aberrations in pluripotent stem cells and somatic cells. The chromatid exchange aberration rates in hESCs increased manifold 2 hours after irradiation as compared with their differentiated derivatives, but the frequency of radiation-induced chromatid breaks was similar. The rate of radiation-induced chromatid exchanges in hESCs and differentiated cells exhibited a quadratic dose response, revealing two-hit mechanism of exchange formation suggesting that a non-homologous end joining (NHEJ) repair may contribute to their formation. Inhibition of DNA-PK, a key NHEJ component, by NU7026 resulted in a significant decrease in radiation-induced c...
Life, 2022
Background: In recent years, the interest in genetic predisposition studies for coronary artery d... more Background: In recent years, the interest in genetic predisposition studies for coronary artery disease and restenosis has increased. Studies show that polymorphisms of genes encoding folate cycle and homocysteine metabolism enzymes significantly contribute to atherogenesis and endothelial dysfunction. The purpose of this study was to examine some SNPs of genes coding for folate cycle enzymes and DNA methyltransferases as risk factors for in-stent restenosis. Methods: The study included 113 patients after stent implantation and 62 patients without signs of coronary artery disease at coronary angiography as the control group. Real-time PCR and RFLP-PCR were applied to genotype all participants for MTHFR rs1801133, MTHFR rs1801131, MTR rs1805087, MTRR rs1801394, DNMT1 rs8101626, DNMT3B rs1569686, and DNMT3B rs2424913 gene polymorphisms. Statistical data processing was carried out using the R language and the SPSS Statistics 20 software. Results: Statistically significant differences i...
Postȩpy higieny i medycyny doświadczalnej (Online), Jan 30, 2010
The renin-angiotensin-aldosterone system (RAAS) plays an important role in the pathogenesis of hy... more The renin-angiotensin-aldosterone system (RAAS) plays an important role in the pathogenesis of hypertension as well as cardiovascular diseases and chronic kidney diseases. Among the most frequently studied RAAS gene polymorphisms are the angiotensin-converting enzyme insertion/deletion (I/D), angiotensinogen M235T and angiotensin II receptor type 1 A1166C polymorphisms. A significant correlation was found between the I/D polymorphism and cardiovascular morbidity and mortality rates. However, there was no significant correlation between I/D, M235T, A1166C polymorphism and arterial hypertension. The role of I/D polymorphism in the development and progression of chronic kidney disease is also non-conclusive. However, DD genotype has been identified as relevant for loss of renal function both in patients with IgA nephropathy and in patients of Asian origin with diabetic nephropathy. The relationship between RAAS gene polymorphism and transplanted kidney function has not been confirmed i...
2 Институт общей генетики им. Н.И.Вавилова, РАН, Москва, Россия. В данной работе исследовали влия... more 2 Институт общей генетики им. Н.И.Вавилова, РАН, Москва, Россия. В данной работе исследовали влияние препарата Кантепарин на цитотокси ческое действие спиртового экстракта болиголова (Сonium maculatum из се мейства зонтичные Umbelliferal) на лимфоцитах человека.Было обнаружено защитное действие препарата Кантепарин, которое проявляется как в сниже нии общего количества гибнущих клеток, так и в уменьшении доли некрозов и, соответственно, увеличении доли апоптозов в спектре индуцированной ги бели клеток.Факт снижения доли некрозов наиболее важен в случае возмож ного применения болиголова или других химиопрепаратов в сочетании с пре паратом Кантепарин в качестве противоопухолевых агентов. Ключевые слова: препарат Кантепарин, цитотоксическое действие, экстракт болиголова, лимфоциты человека.