Dr. Bhupendra K Kumawat | Rajasthan University of Health Sciences, Jaipur (original) (raw)

Papers by Dr. Bhupendra K Kumawat

Powdered pods of Prosopis cineraria (L.) Druce (Fabaceae) were subjected to successive soxhlet ex... more Powdered pods of Prosopis cineraria (L.) Druce (Fabaceae) were subjected to successive soxhlet extraction with petroleum ether (60-800C), benzene, chloroform, alcohol and water to get their respective extracts for detailed Preliminary phytochemical analysis. Fluorescence character of different extracts and pods powder with various reagents were noted under UV and under normal ordinary light. The total ash value, acid insoluble ash value, water soluble ash and sulphated ash value were 4.602, 0.551, 2.420 and 4.611 w/w %, respectively. Loss of weight on drying was 10.317%, the percentage yield for petroleum ether (60-800C), benzene, chloroform, alcohol, water extracts were 1.09, 0.63, 0.59, 3.50 and 15.41w/w %, respectively. The qualitative chemical analysis of extracts were found positive for alkaloids, proteins, carbohydrates, flavonoids, glycosides, saponins and tannins in alcohol and aqueous solvent extracts. These studies provide referential information for correct identification...

World Journal of Pharmaceutical Research, 2017

Microscopical investigation was carried out on leaves of Balanites Aegyptiaca (L.) Delile belongi... more Microscopical investigation was carried out on leaves of Balanites Aegyptiaca (L.) Delile belonging to family Zygophyllaceae, also known as 'Desert date' in English. Literature survey revealed that not much work has been done on this plant, especially on leaves. So we have taken its detailed microscopical studies to prove its appropriate identification. Microscopical study provide information that the leaf exhibits dorsiventaral midrib and smooth, even and thick lamina. The lamina is 350 μm thick. It is isolateral. The marginal part of the lamina is bluntly conical measuring 200 μm thick. Calcium oxlate crystals are abundant in the mesophyll tissue. The crystals are druses and rosette types. The veins form well defined vein-islets of polygonal outline. The vein terminations are well developed and thick, short and straight. At the end of the vein termination occurs a cluster of squarish, thick walled wide sclereids. The leaf is amphistomatic, However, the stomata on the abaxial side are more in frequency than on the adaxial side. The abaxial epidermal cells are small, polygonal and fairly thick walled. The anticlinal walls are straight. The trichomes are unicellular, unbranched and thick walled. Crystal bodies are wide spread in the powder. The crystals are exclusively druses, which are spherical bodies comprising many pointed triangular small crystal units. The above studies provide useful information in regard to its correct identity, evaluation and help to differentiate from the closely related other species of Balanites Aegyptiaca (L.) Delile.

International journal of pharma and bio sciences, 2012

Limonia acidissima, Linn belonging to family Rutaceae commonly known as wood apple and elephant a... more Limonia acidissima, Linn belonging to family Rutaceae commonly known as wood apple and elephant apple has been studied to compare and give detailed reports on pharmacognostical studies like macroscopical and microscopical characters, powder microscopy, physicochemical constants like total a sh value (10.00 %w/w), acid insoluble ash value (5.5%w/w), water soluble ash value (2.16%w/w), sulphated ash value (16.30%w/w), water soluble extractive value (5.7%w/w), ethanol soluble extractive value (2.7%w/w), loss on drying (7.55 %w/w) and fluorescence analysis. Bark shows microscopic characters like cork, cortex, medullary rays, sclerenchyma fibres, phloem, cambium and crystals.The above studies provide useful information in regard to its correct identity and evaluation, and help to differentiate from the closely related other species of Limonia acidissima, Linn.

Chemistry and Chemical Technology, 2019

The study of antioxidants and free radicals in biology is producing medical revolution that promi... more The study of antioxidants and free radicals in biology is producing medical revolution that promises a new age disease and health management. The present study was performed to evaluate the in vitro antioxidant effect of the methanol, ethyl acetate, hydro alcohol and water extracts of Albizia lebbeck (L.) Benth. by using DPPH method. The ethyl acetate extract exhibited significant inhibition in DPPH free radical formation with IC 50 values of 70.5206. The ethyl acetate extract showed potent activity on DPPH, which is compared to that of ascorbic acid (IC 50 = 36.8582) taken as standard. The lesser IC 50 value indicates larger antioxidant activity. The results of the present comprehensive analysis demonstrated that ethyl acetate extract of Albizia lebbeck (L.) Benth. is a viable source of nature antioxidant and might be exploited for functional foods and nutraceutical applications.

Balanites Aegyptiaca (L.) Delile, known as 'desert date' in English, belongs to family Zy... more Balanites Aegyptiaca (L.) Delile, known as 'desert date' in English, belongs to family Zygophyllaceae. This tree is native to much of Africa and parts of the Middle East. In India, It is particularly found in drier parts of Rajasthan, Gujarat, Madhya Pradesh and deccan. The tree can grow up to 10 meters in height with spiny branches, compound leaves and greenish yellow flowers and pale brown date-like fruits. It is highly resistant to stresses such as sandstorms and heat waves, and grows with minimal available moisture. The bark, unripe fruits, and leaves of this plant are reported to have anthelmintic, antifertility, purgative and antidysentric properties. The usefulness of this plant is described in many folk books including Ayurveda and different biologically active phytoconstituents were isolated from plant. But no reports are available on phytochemical studies, hence present work was undertaken to investigate the preliminary phytochemical and Physico-chemical studies on...

Pharmacognosy Journal, 2011

ABSTRACT The anti diarrhoeal activity of aqueous and alcoholic extracts of stem bark of Limonia a... more ABSTRACT The anti diarrhoeal activity of aqueous and alcoholic extracts of stem bark of Limonia acidissima Linn. was evaluated using model of castor oil induced diarrhoeal in mice and gastro intestinal motility test in rats. The results were compared with a standard drug diphenoxylate for castor oil induced diarrhoeal model and atropine sulphate for intestinal motility test. The alcoholic extract of (200 mg/kg) showed significant inhibition in the frequency of defecation, reduction in number of wet faeces dropping and weight of stools as well as decreased the propulsion of charcoal meal through the gastrointestinal tract. Thus the results obtained in present studies suggest that the alcoholic extract have potential of an antidiarrhoeal activity in both methods as compared to standard drug .There is a need to pursue the work further to optimize this lead .

Indian Journal of Pharmaceutical and Biological Research

Microscopical investigation was carried out on leaves of Maytenus emarginata (Willd.) Ding Hou be... more Microscopical investigation was carried out on leaves of Maytenus emarginata (Willd.) Ding Hou belongs to family celastraceae, is an evergreen tree that tolerates various types of stresses of the desert, locally known as “Kankero” in Hindi, “Thorny staff tree” in English. Literature survey revealed that not much work has been done on this plant, especially on leaves. So we have taken its detailed microscopical studies to prove its appropriate identification. Microscopical study provide information that the lamina is dorsiventaral and 380µm thick. The Lamina is amphistomatic, the stomata being distributed both on the adaxial and abaxial sides. Calcium oxalate crystals of druses are fairly abundant in the mesophyll tissue. The stomata are cyclocytic type. The venation is densely reticulate. The terminations are either simple or more commonly forked into deuse clusters. Powder Microscopy show that the sclereids are fiber like in shape and size. The sclereids are 500 µm long and 30 µm t...

Int J Pharm Bio Sci, 2012

Limonia acidissima, Linn belonging to family Rutaceae commonly known as wood apple and elephant a... more Limonia acidissima, Linn belonging to family Rutaceae commonly known as wood apple and elephant apple has been studied to compare and give detailed reports on pharmacognostical studies like macroscopical and microscopical characters, powder microscopy, physico-chemical constants like total ash value (10.00 %w/w), acid insoluble ash value (5.5%w/w), water soluble ash value (2.16%w/w), sulphated ash value (16.30%w/w), water soluble extractive value (5.7%w/w), ethanol soluble extractive value (2.7%w/w), loss on drying (7.55 %w/w) and fluorescence analysis. Bark shows microscopic characters like cork, cortex, medullary rays, sclerenchyma fibres, phloem, cambium and crystals.The above studies provide useful information in regard to its correct identity and evaluation, and help to differentiate from the closely related other species of Limonia acidissima, Linn.

International Journal of …, Dec 2012

scholarsresearchlibrary.com, 2012

Powdered pods of Prosopis cineraria (L.) Druce (Fabaceae) were subjected to successive soxhlet ex... more Powdered pods of Prosopis cineraria (L.) Druce (Fabaceae) were subjected to successive soxhlet extraction with petroleum ether (60-800C), benzene, chloroform, alcohol and water to get their respective extracts for detailed Preliminary phytochemical analysis. Fluorescence character of different extracts and pods powder with various reagents were noted under UV and under normal ordinary light. The total ash value, acid insoluble ash value, water soluble ash and sulphated ash value were 4.602, 0.551, 2.420 and 4.611 w/w %, respectively. Loss of weight on drying was 10.317%, the percentage yield for petroleum ether (60-800C), benzene, chloroform, alcohol, water extracts were 1.09, 0.63, 0.59, 3.50 and 15.41w/w %, respectively. The qualitative chemical analysis of extracts were found positive for alkaloids, proteins, carbohydrates, flavonoids, glycosides, saponins and tannins in alcohol and aqueous solvent extracts. These studies provide referential information for correct identification...

World Journal of Pharmaceutical Research, 2017

Microscopical investigation was carried out on leaves of Balanites Aegyptiaca (L.) Delile belongi... more Microscopical investigation was carried out on leaves of Balanites Aegyptiaca (L.) Delile belonging to family Zygophyllaceae, also known as 'Desert date' in English. Literature survey revealed that not much work has been done on this plant, especially on leaves. So we have taken its detailed microscopical studies to prove its appropriate identification. Microscopical study provide information that the leaf exhibits dorsiventaral midrib and smooth, even and thick lamina. The lamina is 350 μm thick. It is isolateral. The marginal part of the lamina is bluntly conical measuring 200 μm thick. Calcium oxlate crystals are abundant in the mesophyll tissue. The crystals are druses and rosette types. The veins form well defined vein-islets of polygonal outline. The vein terminations are well developed and thick, short and straight. At the end of the vein termination occurs a cluster of squarish, thick walled wide sclereids. The leaf is amphistomatic, However, the stomata on the abaxial side are more in frequency than on the adaxial side. The abaxial epidermal cells are small, polygonal and fairly thick walled. The anticlinal walls are straight. The trichomes are unicellular, unbranched and thick walled. Crystal bodies are wide spread in the powder. The crystals are exclusively druses, which are spherical bodies comprising many pointed triangular small crystal units. The above studies provide useful information in regard to its correct identity, evaluation and help to differentiate from the closely related other species of Balanites Aegyptiaca (L.) Delile.

International journal of pharma and bio sciences, 2012

Limonia acidissima, Linn belonging to family Rutaceae commonly known as wood apple and elephant a... more Limonia acidissima, Linn belonging to family Rutaceae commonly known as wood apple and elephant apple has been studied to compare and give detailed reports on pharmacognostical studies like macroscopical and microscopical characters, powder microscopy, physicochemical constants like total a sh value (10.00 %w/w), acid insoluble ash value (5.5%w/w), water soluble ash value (2.16%w/w), sulphated ash value (16.30%w/w), water soluble extractive value (5.7%w/w), ethanol soluble extractive value (2.7%w/w), loss on drying (7.55 %w/w) and fluorescence analysis. Bark shows microscopic characters like cork, cortex, medullary rays, sclerenchyma fibres, phloem, cambium and crystals.The above studies provide useful information in regard to its correct identity and evaluation, and help to differentiate from the closely related other species of Limonia acidissima, Linn.

Chemistry and Chemical Technology, 2019

The study of antioxidants and free radicals in biology is producing medical revolution that promi... more The study of antioxidants and free radicals in biology is producing medical revolution that promises a new age disease and health management. The present study was performed to evaluate the in vitro antioxidant effect of the methanol, ethyl acetate, hydro alcohol and water extracts of Albizia lebbeck (L.) Benth. by using DPPH method. The ethyl acetate extract exhibited significant inhibition in DPPH free radical formation with IC 50 values of 70.5206. The ethyl acetate extract showed potent activity on DPPH, which is compared to that of ascorbic acid (IC 50 = 36.8582) taken as standard. The lesser IC 50 value indicates larger antioxidant activity. The results of the present comprehensive analysis demonstrated that ethyl acetate extract of Albizia lebbeck (L.) Benth. is a viable source of nature antioxidant and might be exploited for functional foods and nutraceutical applications.

Balanites Aegyptiaca (L.) Delile, known as 'desert date' in English, belongs to family Zy... more Balanites Aegyptiaca (L.) Delile, known as 'desert date' in English, belongs to family Zygophyllaceae. This tree is native to much of Africa and parts of the Middle East. In India, It is particularly found in drier parts of Rajasthan, Gujarat, Madhya Pradesh and deccan. The tree can grow up to 10 meters in height with spiny branches, compound leaves and greenish yellow flowers and pale brown date-like fruits. It is highly resistant to stresses such as sandstorms and heat waves, and grows with minimal available moisture. The bark, unripe fruits, and leaves of this plant are reported to have anthelmintic, antifertility, purgative and antidysentric properties. The usefulness of this plant is described in many folk books including Ayurveda and different biologically active phytoconstituents were isolated from plant. But no reports are available on phytochemical studies, hence present work was undertaken to investigate the preliminary phytochemical and Physico-chemical studies on...

Pharmacognosy Journal, 2011

ABSTRACT The anti diarrhoeal activity of aqueous and alcoholic extracts of stem bark of Limonia a... more ABSTRACT The anti diarrhoeal activity of aqueous and alcoholic extracts of stem bark of Limonia acidissima Linn. was evaluated using model of castor oil induced diarrhoeal in mice and gastro intestinal motility test in rats. The results were compared with a standard drug diphenoxylate for castor oil induced diarrhoeal model and atropine sulphate for intestinal motility test. The alcoholic extract of (200 mg/kg) showed significant inhibition in the frequency of defecation, reduction in number of wet faeces dropping and weight of stools as well as decreased the propulsion of charcoal meal through the gastrointestinal tract. Thus the results obtained in present studies suggest that the alcoholic extract have potential of an antidiarrhoeal activity in both methods as compared to standard drug .There is a need to pursue the work further to optimize this lead .

Indian Journal of Pharmaceutical and Biological Research

Microscopical investigation was carried out on leaves of Maytenus emarginata (Willd.) Ding Hou be... more Microscopical investigation was carried out on leaves of Maytenus emarginata (Willd.) Ding Hou belongs to family celastraceae, is an evergreen tree that tolerates various types of stresses of the desert, locally known as “Kankero” in Hindi, “Thorny staff tree” in English. Literature survey revealed that not much work has been done on this plant, especially on leaves. So we have taken its detailed microscopical studies to prove its appropriate identification. Microscopical study provide information that the lamina is dorsiventaral and 380µm thick. The Lamina is amphistomatic, the stomata being distributed both on the adaxial and abaxial sides. Calcium oxalate crystals of druses are fairly abundant in the mesophyll tissue. The stomata are cyclocytic type. The venation is densely reticulate. The terminations are either simple or more commonly forked into deuse clusters. Powder Microscopy show that the sclereids are fiber like in shape and size. The sclereids are 500 µm long and 30 µm t...

Int J Pharm Bio Sci, 2012

Limonia acidissima, Linn belonging to family Rutaceae commonly known as wood apple and elephant a... more Limonia acidissima, Linn belonging to family Rutaceae commonly known as wood apple and elephant apple has been studied to compare and give detailed reports on pharmacognostical studies like macroscopical and microscopical characters, powder microscopy, physico-chemical constants like total ash value (10.00 %w/w), acid insoluble ash value (5.5%w/w), water soluble ash value (2.16%w/w), sulphated ash value (16.30%w/w), water soluble extractive value (5.7%w/w), ethanol soluble extractive value (2.7%w/w), loss on drying (7.55 %w/w) and fluorescence analysis. Bark shows microscopic characters like cork, cortex, medullary rays, sclerenchyma fibres, phloem, cambium and crystals.The above studies provide useful information in regard to its correct identity and evaluation, and help to differentiate from the closely related other species of Limonia acidissima, Linn.

International Journal of …, Dec 2012

scholarsresearchlibrary.com, 2012