Amal Wageesha | Sabaragamuwa University of Sri Lanka (original) (raw)
Papers by Amal Wageesha
Hypercarotenaemia can occur at any age but it is more commonly seen in infants and young children... more Hypercarotenaemia can occur at any age but it is more commonly seen in infants and young children due to the excessive intake of carotenoid bearing food. The objective of this study was to induce hypercarotenaemia and trace the fate of excess carotenoids in Wistar rats and ICR mice. Wistar rats (n=20) and ICR mice (n=28) were used. Rats and mice were divided into two groups (Test and Control). The controls were fed with standard rat/mice pellets while test group was fed with freeze-dried carrot incorporated standard rat/mice feed with boiled carrot. After a month and 2.5 months, blood was drawn for analyses of carotenoids and metabolites and after 2.5 months liver, adipose and digesta of rats were collected. faeces were freeze dried and then analyzed for carotenoids of metabolites (RP-HPLC). Serum, adipose, liver and bile of test and control mice were also analyzed as above. Wistar rats and ICR mice fed on excess carrot and papaw did not show outward signs of hypercarotenaemia. Thei...
Chemistry Central journal, 2017
All in-text references underlined in blue are linked to publications on ResearchGate, letting you... more All in-text references underlined in blue are linked to publications on ResearchGate, letting you access and read them immediately.
1Department of Biochemistry and Chemistry, Faculty of Medicine, South Asian Institute of Technolo... more 1Department of Biochemistry and Chemistry, Faculty of Medicine, South Asian Institute of Technology and Medicine, Malabe, Sri Lanka. *2Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Colombo, Sri Lanka. 3Hussain Ebrahim Jamal Research Institute of Chemistry, Karachi, Pakistan. 4Department of Biochemistry, Faculty of science, King Abdulaziz University, Saudi Arabia. 5No: 9, Moragahapitiya, Balagola, Kengalle, Kandy, Sri Lanka.
International journal of food sciences and nutrition, 2011
Hypercarotenemia is diagnosed by yellowing of skin. The present study was carried out to study th... more Hypercarotenemia is diagnosed by yellowing of skin. The present study was carried out to study the carotenoids, their metabolites and the vitamin A levels in hypercarotenemics on reporting, changes in serum carotenoids following cessation of feeding carotenoid-bearing foods, and to determine the carotenoids in stools of hypercarotenemics and non-hypercarotenemics. Hypercarotenemic subjects (n = 35) were tested on reporting for a 2-month to 3-month period. Feces from hypercarotenemics (n = 5) and non-hypercarotenemics (n = 8) were extracted and subjected to reverse phase-high-performance liquid chromatography. A questionnaire was administered to parents (n = 35) of these hypercarotenemic children. The serum α- and β carotenoids varied from 119 g/dl to trace and from 149 g/dl to trace respectively, with the monohydroxy metabolites varying from 214 g/dl to nondetectable and polyhydroxy metabolites from 823 g/dl to 7.0 g/dl. Longitudinal studies indicated that serum carotenoid levels de...
International Food Research Journal
Hypercarotenaemia is seen more frequently among young infants and children. This condition develo... more Hypercarotenaemia is seen more frequently among young infants and children. This condition develops mainly due to excessive intake of carotenoid bearing foods. Evidence shows that, not all infants develop hypercarotenaemia due to excessive intake of carotenoid containing foods. The objective of this study was to study the prevalence of hypercarotenaemia among nursery/kindergarten children in the Western province of Sri Lanka and to correlate the food intake with development of hypercarotenaemia. The occurrence of hypercarotenaemia among their siblings fed similar diets was also observed. A self administrated questionnaire was given to the parents (n = 780) of nursery/kindergarten children (2-5 years) in the Western province to collect information on the intake of carotenoid rich foods and development of hypercarotenaemia among the children attending these nurseries and their siblings. Among all the subjects investigated twelve (n = 12) had developed hypercarotenaemia. Children fed w...
International Journal of Food Sciences and Nutrition, 2011
Hypercarotenemia is diagnosed by yellowing of skin. The present study was carried out to study th... more Hypercarotenemia is diagnosed by yellowing of skin. The present study was carried out to study the carotenoids, their metabolites and the vitamin A levels in hypercarotenemics on reporting, changes in serum carotenoids following cessation of feeding carotenoid-bearing foods, and to determine the carotenoids in stools of hypercarotenemics and non-hypercarotenemics. Hypercarotenemic subjects (n = 35) were tested on reporting for a 2-month to 3-month period. Feces from hypercarotenemics (n = 5) and non-hypercarotenemics (n = 8) were extracted and subjected to reverse phase-high-performance liquid chromatography. A questionnaire was administered to parents (n = 35) of these hypercarotenemic children. The serum α- and β carotenoids varied from 119 g/dl to trace and from 149 g/dl to trace respectively, with the monohydroxy metabolites varying from 214 g/dl to nondetectable and polyhydroxy metabolites from 823 g/dl to 7.0 g/dl. Longitudinal studies indicated that serum carotenoid levels declined while vitamin A levels were maintained. α-Carotenes and β-carotenes were not detected in the feces of hypercarotenemics but were present in non-hypercarotenemics.
International Journal of Food Sciences and Nutrition
Hypercarotenemia is diagnosed by yellowing of skin. The present study was carried out to study th... more Hypercarotenemia is diagnosed by yellowing of skin. The present study was carried out to study the carotenoids, their metabolites and the vitamin A levels in hypercarotenemics on reporting, changes in serum carotenoids following cessation of feeding carotenoid-bearing foods, and to determine the carotenoids in stools of hypercarotenemics and non-hypercarotenemics. Hypercarotenemic subjects (n = 35) were tested on reporting for a 2-month to 3-month period. Feces from hypercarotenemics (n = 5) and non-hypercarotenemics (n = 8) were extracted and subjected to reverse phase-high-performance liquid chromatography. A questionnaire was administered to parents (n = 35) of these hypercarotenemic children. The serum α- and β carotenoids varied from 119 g/dl to trace and from 149 g/dl to trace respectively, with the monohydroxy metabolites varying from 214 g/dl to nondetectable and polyhydroxy metabolites from 823 g/dl to 7.0 g/dl. Longitudinal studies indicated that serum carotenoid levels de...
International Food Research Journal
Hypercarotenaemia is seen more frequently among young infants and children. This condition develo... more Hypercarotenaemia is seen more frequently among young infants and children. This condition develops mainly due to excessive intake of carotenoid bearing foods. Evidence shows that, not all infants develop hypercarotenaemia due to excessive intake of carotenoid containing foods. The objective of this study was to study the prevalence of hypercarotenaemia among nursery/kindergarten children in the Western province of Sri Lanka and to correlate the food intake with development of hypercarotenaemia. The occurrence of hypercarotenaemia among their siblings fed similar diets was also observed. A self administrated questionnaire was given to the parents (n = 780) of nursery/kindergarten children (2-5 years) in the Western province to collect information on the intake of carotenoid rich foods and development of hypercarotenaemia among the children attending these nurseries and their siblings. Among all the subjects investigated twelve (n = 12) had developed hypercarotenaemia. Children fed w...
Chemistry Central Journal, Jan 3, 2017
Le Pana Guliya" (LPG) is a polyherbal formulation which is used to treat different types of cance... more Le Pana Guliya" (LPG) is a polyherbal formulation which is used to treat different types of cancers in traditional medicine. In this study we describe in vitro efficacy and mechanism of action of LPG on two cancer cell lines (HepG 2 and HeLa) compared with a normal cell line CC1. The MTT, LDH assays and protein synthesis were used to study antiproliferative activity of LPG while NO synthesis and GSH content were assayed to determine the oxidative stress exerted by LPG. Rhodamine 123 staining, caspase 3 activity, DNA fragmentation and microscopic examination of cells stained with ethidium bromide/acridine orange were used to identify the apoptosis mechanisms associated with LPG. The LPG showed the most potent antiproliferative effect against the proliferation of HepG 2 and HeLa cells with an EC 50 value of 2.72 ± 1.36 and 19.03 ± 2.63 µg/mL for MTT assay after 24 h treatment respectively. In contrast, CC1 cells showed an EC 50 value of 213.07 ± 7.71 µg/mL. Similar results were observed for LDH release. A dose dependent decrease in protein synthesis was shown in both cancer cell types compared to CC1 cells. The reduction of GSH content and elevation of cell survival with exogenous GSH prove that the LPG act via induction of oxidative stress. LPG also stimulates the production of NO and mediates oxidative stress. Rhodamine 123 assay shows the mitochondrial involvement in cell death by depletion of Δψ inducing downstream events in apoptosis. This results in increase in caspase-3 activity eventually DNA fragmentation and LPG induced apoptotic cell death. In conclusion the present study suggested that the LPG exerted an anticancer activity via oxidative stress dependent apoptosis. Therefore present study provides the scientific proof of the traditional knowledge in using LPG as an anticancer agent.
Hypercarotenaemia can occur at any age but it is more commonly seen in infants and young children... more Hypercarotenaemia can occur at any age but it is more commonly seen in infants and young children due to the excessive intake of carotenoid bearing food. The objective of this study was to induce hypercarotenaemia and trace the fate of excess carotenoids in Wistar rats and ICR mice. Wistar rats (n=20) and ICR mice (n=28) were used. Rats and mice were divided into two groups (Test and Control). The controls were fed with standard rat/mice pellets while test group was fed with freeze-dried carrot incorporated standard rat/mice feed with boiled carrot. After a month and 2.5 months, blood was drawn for analyses of carotenoids and metabolites and after 2.5 months liver, adipose and digesta of rats were collected. faeces were freeze dried and then analyzed for carotenoids of metabolites (RP-HPLC). Serum, adipose, liver and bile of test and control mice were also analyzed as above. Wistar rats and ICR mice fed on excess carrot and papaw did not show outward signs of hypercarotenaemia. Thei...
Hypercarotenaemia can occur at any age but it is more commonly seen in infants and young children... more Hypercarotenaemia can occur at any age but it is more commonly seen in infants and young children due to the excessive intake of carotenoid bearing food. The objective of this study was to induce hypercarotenaemia and trace the fate of excess carotenoids in Wistar rats and ICR mice. Wistar rats (n=20) and ICR mice (n=28) were used. Rats and mice were divided into two groups (Test and Control). The controls were fed with standard rat/mice pellets while test group was fed with freeze-dried carrot incorporated standard rat/mice feed with boiled carrot. After a month and 2.5 months, blood was drawn for analyses of carotenoids and metabolites and after 2.5 months liver, adipose and digesta of rats were collected. faeces were freeze dried and then analyzed for carotenoids of metabolites (RP-HPLC). Serum, adipose, liver and bile of test and control mice were also analyzed as above. Wistar rats and ICR mice fed on excess carrot and papaw did not show outward signs of hypercarotenaemia. Thei...
Chemistry Central journal, 2017
All in-text references underlined in blue are linked to publications on ResearchGate, letting you... more All in-text references underlined in blue are linked to publications on ResearchGate, letting you access and read them immediately.
1Department of Biochemistry and Chemistry, Faculty of Medicine, South Asian Institute of Technolo... more 1Department of Biochemistry and Chemistry, Faculty of Medicine, South Asian Institute of Technology and Medicine, Malabe, Sri Lanka. *2Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Colombo, Sri Lanka. 3Hussain Ebrahim Jamal Research Institute of Chemistry, Karachi, Pakistan. 4Department of Biochemistry, Faculty of science, King Abdulaziz University, Saudi Arabia. 5No: 9, Moragahapitiya, Balagola, Kengalle, Kandy, Sri Lanka.
International journal of food sciences and nutrition, 2011
Hypercarotenemia is diagnosed by yellowing of skin. The present study was carried out to study th... more Hypercarotenemia is diagnosed by yellowing of skin. The present study was carried out to study the carotenoids, their metabolites and the vitamin A levels in hypercarotenemics on reporting, changes in serum carotenoids following cessation of feeding carotenoid-bearing foods, and to determine the carotenoids in stools of hypercarotenemics and non-hypercarotenemics. Hypercarotenemic subjects (n = 35) were tested on reporting for a 2-month to 3-month period. Feces from hypercarotenemics (n = 5) and non-hypercarotenemics (n = 8) were extracted and subjected to reverse phase-high-performance liquid chromatography. A questionnaire was administered to parents (n = 35) of these hypercarotenemic children. The serum α- and β carotenoids varied from 119 g/dl to trace and from 149 g/dl to trace respectively, with the monohydroxy metabolites varying from 214 g/dl to nondetectable and polyhydroxy metabolites from 823 g/dl to 7.0 g/dl. Longitudinal studies indicated that serum carotenoid levels de...
International Food Research Journal
Hypercarotenaemia is seen more frequently among young infants and children. This condition develo... more Hypercarotenaemia is seen more frequently among young infants and children. This condition develops mainly due to excessive intake of carotenoid bearing foods. Evidence shows that, not all infants develop hypercarotenaemia due to excessive intake of carotenoid containing foods. The objective of this study was to study the prevalence of hypercarotenaemia among nursery/kindergarten children in the Western province of Sri Lanka and to correlate the food intake with development of hypercarotenaemia. The occurrence of hypercarotenaemia among their siblings fed similar diets was also observed. A self administrated questionnaire was given to the parents (n = 780) of nursery/kindergarten children (2-5 years) in the Western province to collect information on the intake of carotenoid rich foods and development of hypercarotenaemia among the children attending these nurseries and their siblings. Among all the subjects investigated twelve (n = 12) had developed hypercarotenaemia. Children fed w...
International Journal of Food Sciences and Nutrition, 2011
Hypercarotenemia is diagnosed by yellowing of skin. The present study was carried out to study th... more Hypercarotenemia is diagnosed by yellowing of skin. The present study was carried out to study the carotenoids, their metabolites and the vitamin A levels in hypercarotenemics on reporting, changes in serum carotenoids following cessation of feeding carotenoid-bearing foods, and to determine the carotenoids in stools of hypercarotenemics and non-hypercarotenemics. Hypercarotenemic subjects (n = 35) were tested on reporting for a 2-month to 3-month period. Feces from hypercarotenemics (n = 5) and non-hypercarotenemics (n = 8) were extracted and subjected to reverse phase-high-performance liquid chromatography. A questionnaire was administered to parents (n = 35) of these hypercarotenemic children. The serum α- and β carotenoids varied from 119 g/dl to trace and from 149 g/dl to trace respectively, with the monohydroxy metabolites varying from 214 g/dl to nondetectable and polyhydroxy metabolites from 823 g/dl to 7.0 g/dl. Longitudinal studies indicated that serum carotenoid levels declined while vitamin A levels were maintained. α-Carotenes and β-carotenes were not detected in the feces of hypercarotenemics but were present in non-hypercarotenemics.
International Journal of Food Sciences and Nutrition
Hypercarotenemia is diagnosed by yellowing of skin. The present study was carried out to study th... more Hypercarotenemia is diagnosed by yellowing of skin. The present study was carried out to study the carotenoids, their metabolites and the vitamin A levels in hypercarotenemics on reporting, changes in serum carotenoids following cessation of feeding carotenoid-bearing foods, and to determine the carotenoids in stools of hypercarotenemics and non-hypercarotenemics. Hypercarotenemic subjects (n = 35) were tested on reporting for a 2-month to 3-month period. Feces from hypercarotenemics (n = 5) and non-hypercarotenemics (n = 8) were extracted and subjected to reverse phase-high-performance liquid chromatography. A questionnaire was administered to parents (n = 35) of these hypercarotenemic children. The serum α- and β carotenoids varied from 119 g/dl to trace and from 149 g/dl to trace respectively, with the monohydroxy metabolites varying from 214 g/dl to nondetectable and polyhydroxy metabolites from 823 g/dl to 7.0 g/dl. Longitudinal studies indicated that serum carotenoid levels de...
International Food Research Journal
Hypercarotenaemia is seen more frequently among young infants and children. This condition develo... more Hypercarotenaemia is seen more frequently among young infants and children. This condition develops mainly due to excessive intake of carotenoid bearing foods. Evidence shows that, not all infants develop hypercarotenaemia due to excessive intake of carotenoid containing foods. The objective of this study was to study the prevalence of hypercarotenaemia among nursery/kindergarten children in the Western province of Sri Lanka and to correlate the food intake with development of hypercarotenaemia. The occurrence of hypercarotenaemia among their siblings fed similar diets was also observed. A self administrated questionnaire was given to the parents (n = 780) of nursery/kindergarten children (2-5 years) in the Western province to collect information on the intake of carotenoid rich foods and development of hypercarotenaemia among the children attending these nurseries and their siblings. Among all the subjects investigated twelve (n = 12) had developed hypercarotenaemia. Children fed w...
Chemistry Central Journal, Jan 3, 2017
Le Pana Guliya" (LPG) is a polyherbal formulation which is used to treat different types of cance... more Le Pana Guliya" (LPG) is a polyherbal formulation which is used to treat different types of cancers in traditional medicine. In this study we describe in vitro efficacy and mechanism of action of LPG on two cancer cell lines (HepG 2 and HeLa) compared with a normal cell line CC1. The MTT, LDH assays and protein synthesis were used to study antiproliferative activity of LPG while NO synthesis and GSH content were assayed to determine the oxidative stress exerted by LPG. Rhodamine 123 staining, caspase 3 activity, DNA fragmentation and microscopic examination of cells stained with ethidium bromide/acridine orange were used to identify the apoptosis mechanisms associated with LPG. The LPG showed the most potent antiproliferative effect against the proliferation of HepG 2 and HeLa cells with an EC 50 value of 2.72 ± 1.36 and 19.03 ± 2.63 µg/mL for MTT assay after 24 h treatment respectively. In contrast, CC1 cells showed an EC 50 value of 213.07 ± 7.71 µg/mL. Similar results were observed for LDH release. A dose dependent decrease in protein synthesis was shown in both cancer cell types compared to CC1 cells. The reduction of GSH content and elevation of cell survival with exogenous GSH prove that the LPG act via induction of oxidative stress. LPG also stimulates the production of NO and mediates oxidative stress. Rhodamine 123 assay shows the mitochondrial involvement in cell death by depletion of Δψ inducing downstream events in apoptosis. This results in increase in caspase-3 activity eventually DNA fragmentation and LPG induced apoptotic cell death. In conclusion the present study suggested that the LPG exerted an anticancer activity via oxidative stress dependent apoptosis. Therefore present study provides the scientific proof of the traditional knowledge in using LPG as an anticancer agent.
Hypercarotenaemia can occur at any age but it is more commonly seen in infants and young children... more Hypercarotenaemia can occur at any age but it is more commonly seen in infants and young children due to the excessive intake of carotenoid bearing food. The objective of this study was to induce hypercarotenaemia and trace the fate of excess carotenoids in Wistar rats and ICR mice. Wistar rats (n=20) and ICR mice (n=28) were used. Rats and mice were divided into two groups (Test and Control). The controls were fed with standard rat/mice pellets while test group was fed with freeze-dried carrot incorporated standard rat/mice feed with boiled carrot. After a month and 2.5 months, blood was drawn for analyses of carotenoids and metabolites and after 2.5 months liver, adipose and digesta of rats were collected. faeces were freeze dried and then analyzed for carotenoids of metabolites (RP-HPLC). Serum, adipose, liver and bile of test and control mice were also analyzed as above. Wistar rats and ICR mice fed on excess carrot and papaw did not show outward signs of hypercarotenaemia. Thei...