Dr. Asmatullah Kaka | Sindh Agriculture University Tandojam-Pakistan (original) (raw)

Papers by Dr. Asmatullah Kaka

University of Sindh Journal of Animal Sciences (USJAS)

For the evaluation of the phenotypic and morphometric characteristics of Kachhi sheep, a total of... more For the evaluation of the phenotypic and morphometric characteristics of Kachhi sheep, a total of 300 adult Kachhi sheep (female240 and male 60) were selected from different study areas (Hyderabad, Matiary, Mirpurkahs, Sanghar, Umerkot and Thaparkar) of Sindh province. Among all the study areas majority of male and female Kachhi sheep physically possessed plain white coat with black and brown (tan) color, compact body shape, coarse type wool, convex face profile, roman nose, small pendulous ears, sloppy rump, dark colored hooves, and small cylindrical tail, compact udder. Moreover, majority of male and female sheep were noted with wattles, remarkably few cases of female and male Kachhi sheep were detected with deviated Morphometric characters. Average live body weight and linear body measurements (chest girth, body length, height at withers, facial length, ear length, head width and tail length) of both sexes, at Matiary area were observed comparatively (P<0.05) higher, followed ...

Pakistan Journal of Zoology

Authors' Contribution SAW and AAM designed the experiments. SAW, QK and AK performed the experime... more Authors' Contribution SAW and AAM designed the experiments. SAW, QK and AK performed the experiments. Data were analyzed by MFH, HS, SK, AS and MJ contributed in reagents/ materials preparation. SAW, AAM and Q wrote the paper.

Journal of Animal Health and Production

Pakistan Journal of Zoology, 2022

Authors' Contribution SY, SE designed the experiment and wrote the manuscript. MM analyzed the da... more Authors' Contribution SY, SE designed the experiment and wrote the manuscript. MM analyzed the data. ZB provided technical support. SQ, YS and S Sheikh conducted the experiment. S Sadaf designed graphical representation. TP was principal investigator and evaluated the article.

University of Sindh Journal of Animal Sciences (USJAS)

About 40 (n=10) ejaculates were taken from four Tharparkar Bulls (A, B, C, D) having age of 4-5 y... more About 40 (n=10) ejaculates were taken from four Tharparkar Bulls (A, B, C, D) having age of 4-5 years, after the collection, ejaculates were examined for initial evaluation i.e. volume, color, pH, motility, wave motion, viability, morphology, membrane integrity and sperm concentration. Ejaculate having ≥ 70% motility, morphology, membrane integrity and viability were processed and extended into Tris based extender (Control) and Soyabean extender with 3, 6, 9, 12, 15ml concentration of soybean milk. frozen thawed samples were evaluated for motility, morphology, viability and membrane integrity. samples diluted in tris extender resulted in48.50±1.2 motility, 65.00±1.5 morphology, 67.25±3.4 viability and 63.75±2.9 membrane integrity. However, In Soyabean based extender at the quantity of9 ml frozen samples improved semen quality parameters 57.75±1.1 motility, 73.00±3.4 morphology, 72.25±0.3 viability and 70.25±1.3 membrane integrity. After lab-based evaluation, samples diluted with Tri...

Pakistan Journal of Zoology, 2020

Canadian Journal of Plant Science, 1967

The effects of daily photoperiod and light intensity on the uptake of radiophosphorus by Chrysant... more The effects of daily photoperiod and light intensity on the uptake of radiophosphorus by Chrysanthemum morifolium ’Princess Anne’ and Pelargonium hortorum ’Irene’ were studied in controlled-environment rooms. The rate of P32 uptake by both species increased with increasing photoperiod over the range 9, 14, and 19 hr per day at an intensity of 1,000 ft-c. The rate of P32 uptake was higher when the 9- and 14-hr photoperiods were started 7 days prior to the application of P32 than when started concurrently with the application of P32. Plants of each species exposed to a 19-hr photoperiod had the same rate of P32 uptake regardless of when the photoperiod was started.There was a progressive increase in the rate of P32 uptake by both species with increasing light intensity over the range of 500, 1,000, and 1,500 ft-c at a daily photoperiod of 17 hr. It is suggested that increased P32 uptake with increased photoperiod and light intensity is related to increased supply of the photosynthate....

Revista Brasileira de Ciência Avícola, 2012

Tropical biomedicine, 2019

A study was conducted for the examination of bacterial species isolated in dogs from Animal Clini... more A study was conducted for the examination of bacterial species isolated in dogs from Animal Clinics of Nanjing Agricultural University, China. Forty nasal swabs were taken from dogs having respiratory signs. Staphylococcus pseudintermedius was the most frequently isolated pathogen (37.50 %) followed by Staphylococcus aureus (18.75%), Streptococcus pluranimalium (10.93%), Streptococcus canis (9.37%), Staphylococcus schleiferi (9.37%), Staphylococcus intermedius (6.25%), Staphylococcus cohnii (4.71%) and Staphylococcus hominis (3.12%). S. pseudintermedius and S. pluranimalium were subjected to commonly used antibiotics for determination of resistant drugs. Antimicrobial resistance in S. pseudintermedius was common in gentamicin (70.83%) and tetracycline (50%) while in S. pluranimalium was common in enrofloxacin (71.42%) and gentamicin (57.14%).

Sindh University Research Journal, 2016

Present research was conducted to investigate the response of commercial phytase enzyme on growth... more Present research was conducted to investigate the response of commercial phytase enzyme on growth performance of Japanese quail. Total 135 day old quail baby chicks were purchased and randomly divided into 4 groups i.e. S1, S2, S3 and S4. S1 served as control whereas, commercial phytase was given at the ratio of 0.3, 0.6 and 0.8 g/kg feed in S2, S3 and S4, respectively. Feed consumption, total weight gain and FCR was significantly (P 0.05) effected by phytase addition. It was concluded from this study that commercial phytase enzyme has beneficial effect on overall performance of Japanese quail.

Pakistan Journal of Zoology, 2020

Authors' Contribution MN and TW conceived and designed the experiments. MN, NR and SA performed t... more Authors' Contribution MN and TW conceived and designed the experiments. MN, NR and SA performed the experiments. MN and AK wrote the paper. DHK and MR checked and formatted the article.

Pakistan Journal of Zoology, 2022

Authors' Contribution AK, FB, ER, and SA conceived and designed the study and collect data. ER,FB... more Authors' Contribution AK, FB, ER, and SA conceived and designed the study and collect data. ER,FB, SA & RHK analyzed the data. ER, FB, SA, TK and NUK wrote the manuscript. RHK interpreted results and reviewed the draft.

Copyright © 2015 Ubedullah Kaka et al.This is an open access article distributed under theCreativ... more Copyright © 2015 Ubedullah Kaka et al.This is an open access article distributed under theCreativeCommonsAttribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Effects of ketamine and lidocaine on electroencephalographic (EEG) changes were evaluated in minimally anaesthetized dogs, subjected to electric stimulus. Six dogs were subjected to six treatments in a crossover design with a washout period of one week. Dogs were subjected to intravenous boluses of lidocaine 2mg/kg, ketamine 3mg/kg, meloxicam 0.2mg/kg, morphine 0.2mg/kg and loading doses of lidocaine 2mg/kg followed by continuous rate infusion (CRI) of 50 and 100mcg/kg/min, and ketamine 3mg/kg followed by CRI of 10 and 50mcg/kg/min. Electroencephalogram was recorded during electrical stimulation prior to any drug treatment (before treatment) and during electrical stimulation following treatment with the drugs (after treatment) under

The effects of adding various concentrations of antioxidant, butylated hydroxytoluene (BHT) on ch... more The effects of adding various concentrations of antioxidant, butylated hydroxytoluene (BHT) on chilled bull semen for 72 h at 4°C in Bioxcell® (BX), Tris egg-yolk- (TEY) and citrate egg-yolk- (CEY) based semen extenders were studied. Twenty-two ejaculates collected from three bulls, each extended using three extenders with BHT at 0 (control), 0.5, 1.0, 1.5, 2.0 and 3.0 mM/mL, were evaluated for sperm quality parameters. Extended semen was packaged into 0.25 mL straws containing 20 x 106 spermatozoa and chilled for 72 h. Four random straws each from the control and treatment groups were warmed at 37°C, pooled and evaluated using a computer-assisted semen analyser (IVOS Hamilton Thorne Biosciences) for general and progressive motilities, and for morphology, viability and acrosome damage using eosin-nigrosin stain under phase-contrast microscope. Results showed that sperm morphology, viability and protection of acrosome damage were significantly improved (p<0.05) at BHT concentratio...

Animal production has been modernized through cryopreservation. However,cryopreservation decrease... more Animal production has been modernized through cryopreservation. However,cryopreservation decreases the quality and fertility of bull sperm by impairing its normal functions due to thermal, oxidative and osmotic changes and re-distribution of lipid bonding. Therefore, the present study was conducted to test, if supplementation of semen extenders with fatty acids and antioxidants could improve the quality of frozenthawed of bull semen. Semen samples from three fertile beef bulls were collected twice a week by an electro ejaculator. After collection, semen samples were brought to the laboratory for initial evaluation. Ejaculates with motility ≥ 70% and normal morphology and viability ≥ 80% were processed for cryopreservation. Ejaculates were extended in Tris and BioXcell® extenders containing 0 (control), 3, 5, 10 and 15ng/ml of docosahexaenoic acid (DHA) and α-linolenic acid (ALA). As the fatty acids are insoluble in water, 0.05% ethanol was added as a solvent. Extended samples were i...

This experiment was conducted to determine the physical and ultra-structural attributes of fresh ... more This experiment was conducted to determine the physical and ultra-structural attributes of fresh bull semen and assess their potential use for freezing and AI. A total of 40 semen samples collected from 4 mature bulls (10 samples per bull) were analyzed. The semen samples were examined for colour, volume, concentration, pH, general and progressive motilities, morphologically normal spermatozoa, acrosome and DNA damage, and lipid peroxidation. Transmission Electron Microscopy (TEM) was also performed to evaluate the ultra-structures of the spermatozoa. Results showed the semen colour varied from bull to bull from creamy-white in bull #1, to milky in bulls #2 and 4, then cloudy in bull #3. Highest sperm concentration, lipid peroxidation and pH were recorded from bull #4. Highest volume, progressive motility, morphology, less acrosome damage and viability were from bull #2. While best values for general motility and DNA damage were obtained from bull #1. TEM revealed 92.5, 90.0 and 82%...

The aim of this study was to evaluate the effects of using honey as a supplement into Tris extend... more The aim of this study was to evaluate the effects of using honey as a supplement into Tris extender in comparison with a commercial Bioxcell ® extender on bull semen cryopreservation quality. Semen samples from four fertile bulls, which comprised of two Friesian Sahiwal, one Braford and one Kedah-Kelantan breeds, were collected using an electro-ejaculation method. Collected samples were freshly evaluated physically and microscopically before divided and diluted using four Tris-based extenders containing 0% (control), 2.5%, 5% and 10% honey, and a commercial Bioxcell ® extender. The processed semen was equilibrated at 5°C for 3 h (chilling), packed into straws (0.25 ml), frozen and stored in a liquid nitrogen tank for 24 h before thawing and assessment of quality. Semen quality parameters used for evaluation and comparison after chilling and thawing included general and progressive motility (analyzed using a computer assisted semen analyzer), livability and sperm morphology (done usi...

1 Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam, Pak... more 1 Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam, Pakistan 2 Animal Reproduction Program, Animal Sciences, National Agriculture Research Centre, Park Road, Islamabad, Pakistan *Corresponding Author’s E-mail: akeelahmedmemon@gmail.com The present study was designed to compare the efficacy of estrus synchronization protocols with or without Biostimulation in Kundhi buffaloes at and surroundings of Kundhi buffalo farm, Rorhi. Selected forty Buffaloes (n=40) of 1 st to 5 th parity were divided into group A (Bull exposed; BE) and B (Non-exposed; NE). These both groups were further subdivided into subgroups (A1 and A2, B1 and B2). Subgroups A1 and B1 received Ovsynch GnRH at day 0 followed by PGF2α day 7 th and second dose of GnRH at day 9 th . Whereas, in subgroups A2 and B2, estrus was synchronized with double PGF2α protocol at interval of 11days. The groups did not show differ (P>0.05) estrus response but found high in A1 (80%) than A2 (70...

The study on in-vitro fertility assessment of frozen thawed semen from Kundhi bulls was undertake... more The study on in-vitro fertility assessment of frozen thawed semen from Kundhi bulls was undertaken during the months of July to September 2006 at the Department of Animal Reproduction, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam. Before freezing, each ejaculate was assessed for volume, color, mass activity, and motility and sperm concentration. Twenty samples having motility 60% or above were frozen for post-thaw assessment. Frozen thawed semen was incubated at 25C for 5 hours and examined for progressive linear motility and live dead sperm count. It was observed that all the ejaculates were creamy white in color. The mean (± SEM) volume, mass activity, motility percentage sperm concentrations and pH of the semen from Kundhi buffalo bulls were found to be 2.79 ± 0.217 ml, 2.85 ± 0.111, 71.75 ± 2.621%, 11.35 ± 1.255 millions/ml and 5.82 ± 0.092 respectively for fresh semen. No significant (P>0.05) difference was found between the para...

April-2020, 2020

Aim: The aim of this study was to investigate the effects of different concentration of butylated... more Aim: The aim of this study was to investigate the effects of different concentration of butylated hydroxytoluene (BHT) on sperm membrane surface protein "P25b" from cryopreserved bull semen in either lecithin based Bioxcell® (BX) or two egg-yolk based extenders, tris-egg yolk (TEY), and citrate-egg yolk (CEY). Materials and Methods: Forty-five semen samples, 15 each were extended with either BX, TEY, or CEY extender which contained different concentrations (0.0 - control, 0.5, 1.0, 1.5, 2.0, and 3.0 mM/mL) of BHT. The extended semen samples were frozen at a concentration of 20×106/mL in 0.25 mL straws and stored in liquid nitrogen for 2 weeks. The frozen samples were thereafter thawed, proteins extracted and analyzed for quantities of protein P25b through direct sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel densitometry. Peptides were confirmed by Western blotting (WB). Results: Results showed that supplementation of BHT improved (p<0.05) quantity of pro...

University of Sindh Journal of Animal Sciences (USJAS)

For the evaluation of the phenotypic and morphometric characteristics of Kachhi sheep, a total of... more For the evaluation of the phenotypic and morphometric characteristics of Kachhi sheep, a total of 300 adult Kachhi sheep (female240 and male 60) were selected from different study areas (Hyderabad, Matiary, Mirpurkahs, Sanghar, Umerkot and Thaparkar) of Sindh province. Among all the study areas majority of male and female Kachhi sheep physically possessed plain white coat with black and brown (tan) color, compact body shape, coarse type wool, convex face profile, roman nose, small pendulous ears, sloppy rump, dark colored hooves, and small cylindrical tail, compact udder. Moreover, majority of male and female sheep were noted with wattles, remarkably few cases of female and male Kachhi sheep were detected with deviated Morphometric characters. Average live body weight and linear body measurements (chest girth, body length, height at withers, facial length, ear length, head width and tail length) of both sexes, at Matiary area were observed comparatively (P<0.05) higher, followed ...

Pakistan Journal of Zoology

Authors' Contribution SAW and AAM designed the experiments. SAW, QK and AK performed the experime... more Authors' Contribution SAW and AAM designed the experiments. SAW, QK and AK performed the experiments. Data were analyzed by MFH, HS, SK, AS and MJ contributed in reagents/ materials preparation. SAW, AAM and Q wrote the paper.

Journal of Animal Health and Production

Pakistan Journal of Zoology, 2022

Authors' Contribution SY, SE designed the experiment and wrote the manuscript. MM analyzed the da... more Authors' Contribution SY, SE designed the experiment and wrote the manuscript. MM analyzed the data. ZB provided technical support. SQ, YS and S Sheikh conducted the experiment. S Sadaf designed graphical representation. TP was principal investigator and evaluated the article.

University of Sindh Journal of Animal Sciences (USJAS)

About 40 (n=10) ejaculates were taken from four Tharparkar Bulls (A, B, C, D) having age of 4-5 y... more About 40 (n=10) ejaculates were taken from four Tharparkar Bulls (A, B, C, D) having age of 4-5 years, after the collection, ejaculates were examined for initial evaluation i.e. volume, color, pH, motility, wave motion, viability, morphology, membrane integrity and sperm concentration. Ejaculate having ≥ 70% motility, morphology, membrane integrity and viability were processed and extended into Tris based extender (Control) and Soyabean extender with 3, 6, 9, 12, 15ml concentration of soybean milk. frozen thawed samples were evaluated for motility, morphology, viability and membrane integrity. samples diluted in tris extender resulted in48.50±1.2 motility, 65.00±1.5 morphology, 67.25±3.4 viability and 63.75±2.9 membrane integrity. However, In Soyabean based extender at the quantity of9 ml frozen samples improved semen quality parameters 57.75±1.1 motility, 73.00±3.4 morphology, 72.25±0.3 viability and 70.25±1.3 membrane integrity. After lab-based evaluation, samples diluted with Tri...

Pakistan Journal of Zoology, 2020

Canadian Journal of Plant Science, 1967

The effects of daily photoperiod and light intensity on the uptake of radiophosphorus by Chrysant... more The effects of daily photoperiod and light intensity on the uptake of radiophosphorus by Chrysanthemum morifolium ’Princess Anne’ and Pelargonium hortorum ’Irene’ were studied in controlled-environment rooms. The rate of P32 uptake by both species increased with increasing photoperiod over the range 9, 14, and 19 hr per day at an intensity of 1,000 ft-c. The rate of P32 uptake was higher when the 9- and 14-hr photoperiods were started 7 days prior to the application of P32 than when started concurrently with the application of P32. Plants of each species exposed to a 19-hr photoperiod had the same rate of P32 uptake regardless of when the photoperiod was started.There was a progressive increase in the rate of P32 uptake by both species with increasing light intensity over the range of 500, 1,000, and 1,500 ft-c at a daily photoperiod of 17 hr. It is suggested that increased P32 uptake with increased photoperiod and light intensity is related to increased supply of the photosynthate....

Revista Brasileira de Ciência Avícola, 2012

Tropical biomedicine, 2019

A study was conducted for the examination of bacterial species isolated in dogs from Animal Clini... more A study was conducted for the examination of bacterial species isolated in dogs from Animal Clinics of Nanjing Agricultural University, China. Forty nasal swabs were taken from dogs having respiratory signs. Staphylococcus pseudintermedius was the most frequently isolated pathogen (37.50 %) followed by Staphylococcus aureus (18.75%), Streptococcus pluranimalium (10.93%), Streptococcus canis (9.37%), Staphylococcus schleiferi (9.37%), Staphylococcus intermedius (6.25%), Staphylococcus cohnii (4.71%) and Staphylococcus hominis (3.12%). S. pseudintermedius and S. pluranimalium were subjected to commonly used antibiotics for determination of resistant drugs. Antimicrobial resistance in S. pseudintermedius was common in gentamicin (70.83%) and tetracycline (50%) while in S. pluranimalium was common in enrofloxacin (71.42%) and gentamicin (57.14%).

Sindh University Research Journal, 2016

Present research was conducted to investigate the response of commercial phytase enzyme on growth... more Present research was conducted to investigate the response of commercial phytase enzyme on growth performance of Japanese quail. Total 135 day old quail baby chicks were purchased and randomly divided into 4 groups i.e. S1, S2, S3 and S4. S1 served as control whereas, commercial phytase was given at the ratio of 0.3, 0.6 and 0.8 g/kg feed in S2, S3 and S4, respectively. Feed consumption, total weight gain and FCR was significantly (P 0.05) effected by phytase addition. It was concluded from this study that commercial phytase enzyme has beneficial effect on overall performance of Japanese quail.

Pakistan Journal of Zoology, 2020

Authors' Contribution MN and TW conceived and designed the experiments. MN, NR and SA performed t... more Authors' Contribution MN and TW conceived and designed the experiments. MN, NR and SA performed the experiments. MN and AK wrote the paper. DHK and MR checked and formatted the article.

Pakistan Journal of Zoology, 2022

Authors' Contribution AK, FB, ER, and SA conceived and designed the study and collect data. ER,FB... more Authors' Contribution AK, FB, ER, and SA conceived and designed the study and collect data. ER,FB, SA & RHK analyzed the data. ER, FB, SA, TK and NUK wrote the manuscript. RHK interpreted results and reviewed the draft.

Copyright © 2015 Ubedullah Kaka et al.This is an open access article distributed under theCreativ... more Copyright © 2015 Ubedullah Kaka et al.This is an open access article distributed under theCreativeCommonsAttribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Effects of ketamine and lidocaine on electroencephalographic (EEG) changes were evaluated in minimally anaesthetized dogs, subjected to electric stimulus. Six dogs were subjected to six treatments in a crossover design with a washout period of one week. Dogs were subjected to intravenous boluses of lidocaine 2mg/kg, ketamine 3mg/kg, meloxicam 0.2mg/kg, morphine 0.2mg/kg and loading doses of lidocaine 2mg/kg followed by continuous rate infusion (CRI) of 50 and 100mcg/kg/min, and ketamine 3mg/kg followed by CRI of 10 and 50mcg/kg/min. Electroencephalogram was recorded during electrical stimulation prior to any drug treatment (before treatment) and during electrical stimulation following treatment with the drugs (after treatment) under

The effects of adding various concentrations of antioxidant, butylated hydroxytoluene (BHT) on ch... more The effects of adding various concentrations of antioxidant, butylated hydroxytoluene (BHT) on chilled bull semen for 72 h at 4°C in Bioxcell® (BX), Tris egg-yolk- (TEY) and citrate egg-yolk- (CEY) based semen extenders were studied. Twenty-two ejaculates collected from three bulls, each extended using three extenders with BHT at 0 (control), 0.5, 1.0, 1.5, 2.0 and 3.0 mM/mL, were evaluated for sperm quality parameters. Extended semen was packaged into 0.25 mL straws containing 20 x 106 spermatozoa and chilled for 72 h. Four random straws each from the control and treatment groups were warmed at 37°C, pooled and evaluated using a computer-assisted semen analyser (IVOS Hamilton Thorne Biosciences) for general and progressive motilities, and for morphology, viability and acrosome damage using eosin-nigrosin stain under phase-contrast microscope. Results showed that sperm morphology, viability and protection of acrosome damage were significantly improved (p<0.05) at BHT concentratio...

Animal production has been modernized through cryopreservation. However,cryopreservation decrease... more Animal production has been modernized through cryopreservation. However,cryopreservation decreases the quality and fertility of bull sperm by impairing its normal functions due to thermal, oxidative and osmotic changes and re-distribution of lipid bonding. Therefore, the present study was conducted to test, if supplementation of semen extenders with fatty acids and antioxidants could improve the quality of frozenthawed of bull semen. Semen samples from three fertile beef bulls were collected twice a week by an electro ejaculator. After collection, semen samples were brought to the laboratory for initial evaluation. Ejaculates with motility ≥ 70% and normal morphology and viability ≥ 80% were processed for cryopreservation. Ejaculates were extended in Tris and BioXcell® extenders containing 0 (control), 3, 5, 10 and 15ng/ml of docosahexaenoic acid (DHA) and α-linolenic acid (ALA). As the fatty acids are insoluble in water, 0.05% ethanol was added as a solvent. Extended samples were i...

This experiment was conducted to determine the physical and ultra-structural attributes of fresh ... more This experiment was conducted to determine the physical and ultra-structural attributes of fresh bull semen and assess their potential use for freezing and AI. A total of 40 semen samples collected from 4 mature bulls (10 samples per bull) were analyzed. The semen samples were examined for colour, volume, concentration, pH, general and progressive motilities, morphologically normal spermatozoa, acrosome and DNA damage, and lipid peroxidation. Transmission Electron Microscopy (TEM) was also performed to evaluate the ultra-structures of the spermatozoa. Results showed the semen colour varied from bull to bull from creamy-white in bull #1, to milky in bulls #2 and 4, then cloudy in bull #3. Highest sperm concentration, lipid peroxidation and pH were recorded from bull #4. Highest volume, progressive motility, morphology, less acrosome damage and viability were from bull #2. While best values for general motility and DNA damage were obtained from bull #1. TEM revealed 92.5, 90.0 and 82%...

The aim of this study was to evaluate the effects of using honey as a supplement into Tris extend... more The aim of this study was to evaluate the effects of using honey as a supplement into Tris extender in comparison with a commercial Bioxcell ® extender on bull semen cryopreservation quality. Semen samples from four fertile bulls, which comprised of two Friesian Sahiwal, one Braford and one Kedah-Kelantan breeds, were collected using an electro-ejaculation method. Collected samples were freshly evaluated physically and microscopically before divided and diluted using four Tris-based extenders containing 0% (control), 2.5%, 5% and 10% honey, and a commercial Bioxcell ® extender. The processed semen was equilibrated at 5°C for 3 h (chilling), packed into straws (0.25 ml), frozen and stored in a liquid nitrogen tank for 24 h before thawing and assessment of quality. Semen quality parameters used for evaluation and comparison after chilling and thawing included general and progressive motility (analyzed using a computer assisted semen analyzer), livability and sperm morphology (done usi...

1 Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam, Pak... more 1 Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam, Pakistan 2 Animal Reproduction Program, Animal Sciences, National Agriculture Research Centre, Park Road, Islamabad, Pakistan *Corresponding Author’s E-mail: akeelahmedmemon@gmail.com The present study was designed to compare the efficacy of estrus synchronization protocols with or without Biostimulation in Kundhi buffaloes at and surroundings of Kundhi buffalo farm, Rorhi. Selected forty Buffaloes (n=40) of 1 st to 5 th parity were divided into group A (Bull exposed; BE) and B (Non-exposed; NE). These both groups were further subdivided into subgroups (A1 and A2, B1 and B2). Subgroups A1 and B1 received Ovsynch GnRH at day 0 followed by PGF2α day 7 th and second dose of GnRH at day 9 th . Whereas, in subgroups A2 and B2, estrus was synchronized with double PGF2α protocol at interval of 11days. The groups did not show differ (P>0.05) estrus response but found high in A1 (80%) than A2 (70...

The study on in-vitro fertility assessment of frozen thawed semen from Kundhi bulls was undertake... more The study on in-vitro fertility assessment of frozen thawed semen from Kundhi bulls was undertaken during the months of July to September 2006 at the Department of Animal Reproduction, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam. Before freezing, each ejaculate was assessed for volume, color, mass activity, and motility and sperm concentration. Twenty samples having motility 60% or above were frozen for post-thaw assessment. Frozen thawed semen was incubated at 25C for 5 hours and examined for progressive linear motility and live dead sperm count. It was observed that all the ejaculates were creamy white in color. The mean (± SEM) volume, mass activity, motility percentage sperm concentrations and pH of the semen from Kundhi buffalo bulls were found to be 2.79 ± 0.217 ml, 2.85 ± 0.111, 71.75 ± 2.621%, 11.35 ± 1.255 millions/ml and 5.82 ± 0.092 respectively for fresh semen. No significant (P>0.05) difference was found between the para...

April-2020, 2020

Aim: The aim of this study was to investigate the effects of different concentration of butylated... more Aim: The aim of this study was to investigate the effects of different concentration of butylated hydroxytoluene (BHT) on sperm membrane surface protein "P25b" from cryopreserved bull semen in either lecithin based Bioxcell® (BX) or two egg-yolk based extenders, tris-egg yolk (TEY), and citrate-egg yolk (CEY). Materials and Methods: Forty-five semen samples, 15 each were extended with either BX, TEY, or CEY extender which contained different concentrations (0.0 - control, 0.5, 1.0, 1.5, 2.0, and 3.0 mM/mL) of BHT. The extended semen samples were frozen at a concentration of 20×106/mL in 0.25 mL straws and stored in liquid nitrogen for 2 weeks. The frozen samples were thereafter thawed, proteins extracted and analyzed for quantities of protein P25b through direct sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel densitometry. Peptides were confirmed by Western blotting (WB). Results: Results showed that supplementation of BHT improved (p<0.05) quantity of pro...

This study was conducted to investigate the effect of docosahexaenoic acid (DHA) supplementation ... more This study was conducted to investigate the effect of docosahexaenoic acid (DHA) supplementation into BioXcell® extender on the quality of frozen-thawed bull semen. Twenty four ejaculates were collected using an electro ejaculator from 3 bulls 8 per bull. Ejaculates with motility ≥70%, normal morphology and viability ≥80% were extended into BioXcell® extender, to which 0 (control), 3,5,10,15 ng/ml of DHA were added. The supplemented semen samples were incubated at 37oC for 15 minutes for DHA uptake by sperm. Later, samples were cooled for 2 hours at 5oC and packaged into 0.25ml straws and frozen in liquid nitrogen for 24hours. Subsequently, straws were thawed and evaluated for total sperm motility (computer assisted semen analysis), membrane functional integrity (Hypo-osmotic swelling test), viability (eosin-nigrosin), and lipid peroxidation (thiobarbituric acid reactive substances, TBARS). Sperm motility (48.94±0.8) was significantly (P<0.05) higher at 3ng/ml whereas normal morphology (67.84±1.5), viability (71.94±2.4), acrosome integrity (75.52±0.8) and membrane integrity (71.36±2.3) produced higher values at 3ng/ml but were not significant (P>0.05) with 5ng/ml. DHA was significantly higher in supplemented groups than control. Lipid peroxidation showed a trend of increment with linear to increment in DHA supplementation. Overall results showed that 3ng/ml DHA to BioXcell® improved frozen-thawed quality of bull sperm.