Abbas Piryaei | Shahid Beheshti University of Medical Sciences (original) (raw)

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Papers by Abbas Piryaei

Laboratory animal research, 2015

The effect of pentoxifylline (PTX) administration on histomorphological parameters of streptozoto... more The effect of pentoxifylline (PTX) administration on histomorphological parameters of streptozotocin (STZ)-induced type 1 diabetes mellitus (DM) in male rat testes were evaluated. We randomly divided 40 male rats into the following four groups: group 1: control or normal glycemic (NG) rats; group 2 or NG rats that received only normal saline (NS), (NG+NS); group 3 or diabetic rats which were not treated by PTX (DM+vehicle solution (NS)); and group 4 which comprised diabetic rats treated with 50 mg/kg of PTX (DM+PTX). Type 1 DM was induced by intraperitoneal injection of STZ (55 mg/kg). Rats were held for 30 days after which the experimental group received PTX twice daily (25 mg/kg) or NS. After 14 days of treatment by PTX or NS, the left testes from all rats were extracted and prpared for histological study. Apoptotic cells, blood vessel density, and spermatogenesis were evaluated. Data were analyzed by ANOVA test. PTX-treated-diabetic rats showed a significant decrease in number of...

Iranian biomedical journal, 2013

Spinal cord has a limited capacity to repair; therefore, medical interventions are necessary for ... more Spinal cord has a limited capacity to repair; therefore, medical interventions are necessary for treatment of injuries. Transplantation of Schwann cells has shown a great promising result for spinal cord injury (SCI). However, harvesting Schwann cell has been limited due to donor morbidity and limited expansion capacity. Furthermore, accessible sources such as bone marrow stem cells have drawn attentions to themselves. Therefore, this study was designed to evaluate the effect of bone marrow-derived Schwann cell on functional recovery in adult rats after injury. Mesenchymal stem cells were cultured from adult rats' bone marrow and induced into Schwann cells in vitro. Differentiation was confirmed by immunocytochemistry and RT-PCR. Next, Schwann cells were seeded into collagen scaffolds and engrafted in 3 mm lateral hemisection defects. For 8 weeks, motor and sensory improvements were assessed by open field locomotor scale, narrow beam, and tail flick tests. Afterwards, lesioned s...

ABSTRACT Liver tissue engineering with stem cell-derived hepatocyte-like cells provides a promisi... more ABSTRACT Liver tissue engineering with stem cell-derived hepatocyte-like cells provides a promising alternative to liver transplantation in patients with acute and chronic hepatic failure. A serious shortage of liver donors, high cost, the need for immunosuppressive medications and the high risk of transplant rejection remain the major limitations in the _eld of liver transplantation. Using cells instead of organs in this setting should permit (i) expansion of cells in an in vitro phase, (ii) genetic or immunological manipulation of cells for transplantation, (iii) tissue typing and cryopreservation in a cell bank and (iv) the ex vivo genetic modi_cation of the patient’s own cells prior to re-implantation. The creation of an unlimited source of donor cells for hepatocyte transplantation therapy, liver tissue engineering and pharmaceutical applications may be the result of isolation and expansion of progenitor cells or stem cells. The cells are required to function in the same manner as normal hepatic cells, or differentiate into hepatocyte-like cells that can replace the function of the failed liver. The hepatogenic potential of many types of stem cells from different sources has been previously described and the majority of them used for this purpose. The clinical impact of tissue engineering depends upon our ability to direct cells to form tissues with characteristic structural and mechanical properties from the molecular level up to organized tissue. In most cases, tissue engineering attempts to recapitulate certain aspects of normal development in order to stimulate cell differentiation and functional tissue assembly. The induction of tissue growth generally involves the use of biodegradable and bioactive materials designed, ideally, to provide a mechanical, physical and biochemical template for tissue regeneration. Function and differentiation of liver cells and stem cell-derived hepatocyte-like cells are in_uenced by the three-dimensional organ architecture. Hepatocytes are attachment-dependent cells and lose their liver-speci_c functions or die without an optimal normal microenvironment, extracellular matrix composition and cell-cell contacts. The use of polymeric matrices and/or nano_brillar components permits the three-dimensional formation of a neo tissue and speci_c stimulation by adequate modi_cation of the matrix surface, which might be essential for appropriate differentiation of transplanted cells. In addition to development of bioarti_cial liver devices (BAL) for extracorporeal liver support and intracorporeal liver replacement, a concept that combines tissue engineering using three-dimensional, highly porous matrices with cell transplantation could be useful. However, the creation and development of a functional liver tissue for transplantation must overcome the limited distance of oxygen diffusion and accessibility to blood plasma. Therefore induction and creation of efcient vascular networks has been one of the fundamental challenges of liver tissue engineering either in vitro, for the transplantation of prevascularized constructs, or in vivo, for cellular organization and transplant incorporation within the implantation site. Vascularization in vitro could maintain cell viability during tissue growth, induce structural organization and promote vascularization into a host tissue upon implantation. In summary, as the liver is a very complex organ and has dual functions of both metabolism and detoxication through incorporation of heterotypic cell-cell interactions, 3D architecture and perfused flow, it is crucial to consider the choice of cell source and culture condition, type of scaffolds and creation of vascular networks for long-term maintenance of tissue-specic functions required to reconstruct a tissue-engineered liver.

ABSTRACT Background: Pentoxifylline decreased blood viscosity, improved peripheral blood circulat... more ABSTRACT Background: Pentoxifylline decreased blood viscosity, improved peripheral blood circulation, and improved red blood cell flexibility. It also induced sperm motility and increased microvascular blood circulation in non-diabetic humans. The aim of present study was to evaluate the effect of pentoxifylline administration on Sertoli and Leydig cells count of experimentally induced type 1 diabetes in male rats. Materials and methods: In this experimental study 50 male rats were randomly divided into 3 nondiabetic groups, and 2 diabetic groups. Type 1 diabetes was induced by injection of streptozotocin, and rats were held for 30 days. Experimental groups on non-diabetic and diabetic rats were received pentoxifylline daily. One non-diabetic group received daily normal saline. Then, all rats were anesthetized and their right testis were extracted and were examined histologically. Sertoli cells and Leydig cells were counted. Data were analyzed by ANOVA and LSD tests. Results: Increase in number of pentoxifylline- treated diabetic rats cells (7.43±0.86, 15.25±1.6) were significantly higher than control diabetic rats (4.4±0.38, 11±1.31) (p=0.001 and p=0.000 respectively). There were significant difference between pentoxifylline treated diabetic rats cells (7.43±0.86, 15.25±1.6), and normal saline- treated non- diabetic rats cells (7.17±1.13, 25.8, 3.5) with other groups (p=0.001 and p=0.000 respectively). There were no significant difference between non- diabetic groups. Conclusion: Our results showed that pentoxifylline administration increased Sertoli cells and Leydig cells count in non-diabetic and diabetic rats in compare to control groups. However its effect in diabetic rats were significant. Keywords: Diabetes Mellitus, Pentoxifylline, Sertoli Cells, Leydig Cells, Rat

ABSTRACT When the spinal cord is damaged, medical procedures are vital to prevent of improvement ... more ABSTRACT When the spinal cord is damaged, medical procedures are vital to prevent of improvement of the lesion. Because of poor regeneration ability of central nervous tissue, the most injuries are irreversible. One of encouraging interventions for treatment of spinal cord injury is Schwann cell transplantation. However, isolation of Schwann cell for clinical interventions is complicated approach with low cells yield and purity. Thus, easily accessed sources like Adipose mesenchymal stem cells have been taken notice. Therefore, this study was planned to assess the effect of adipose stromal cell-derived Schwann cell transplantation in functional recovery after lateral hemisection in adult rats. After isolation, adipose stem cells were differentiated to Schwann cells. The differentiation was verified by immunocytochemistry and reverse transcription-polymerase chain reaction (RT-PCR). Then, we loaded the cells into collagen scaffolds with parallel aligned canals and transplanted into rats with 3 mm lesions at T9 - T10 level. Motor and sensory improvement were evaluated by open field locomotor scale, narrow beam, and tail flick tests for 60 days. Subsequently, conventional histology and immunohistochemistry were performed. In vitro results revealed that mesenchymal stem cells after differentiation gained Schwann cells morphology and markers. Schwann cell-grafted group had significantly higher locomotor and sensory scores in comparison with the control and scaffold without cell groups. Histological observations showed differentiated cells have the ability to improve axonal regeneration and remyelination. Our study proved that adipose tissue- derived Schwann cells can change the rough environment of damaged spinal cord and support axon regeneration and enhance functional recovery, and possibly be helpful for people suffering from spinal cord injuries.

Biomaterials, 2009

The interactions of bone marrow-derived mesenchymal stem cells (MSCs) and their engrafted microen... more The interactions of bone marrow-derived mesenchymal stem cells (MSCs) and their engrafted microenvironment are an integral part of signaling control of stem cell lineage commitment. We attempted to induce bone marrow-derived MSCs to undergo epidermal lineage differentiation by manipulating the biochemical, environmental and physical properties of culture conditions in an organotypic coculture model to simulate a skin-specific microenvironment. The induction medium was optimized by varying different biomolecular supplements in a basic stratification medium. A multi-layered epidermis-like structure was established when MSCs were cultured in an optimized induction medium on a contractible fibroblast-embedded collagen gel with an air-liquid interface. The commitment into epidermal lineage was further confirmed by the expression of early and intermediate epidermalization markers - keratin 10 and filaggrin in 90.67% and 80.51% of MSCs, respectively. This study not only highlights the possibility of in vitro control of MSCs into epidermal lineage, but also suggests the therapeutic potential of bone marrow-derived MSCs for skin regeneration.

Genetics and Molecular Biology, 2015

Nuclear transfer embryonic stem cells (ntESCs) show stem cell characteristics such as pluripotenc... more Nuclear transfer embryonic stem cells (ntESCs) show stem cell characteristics such as pluripotency but cause no immunological disorders. Although ntESCs are able to differentiate into somatic cells, the ability of ntESCs to differentiate into primordial germ cells (PGCs) has not been examined. In this work, we examined the capacity of mouse ntESCs to differentiate into PGCs in vitro. ntESCs aggregated to form embryoid bodies (EB) in EB culture medium supplemented with bone morphogenetic protein 4(BMP4) as the differentiation factor. The expression level of specific PGC genes was compared at days 4 and 8 using real time PCR. Flow cytometry and immunocytochemical staining were used to detect Mvh as a specific PGC marker. ntESCs expressed particular genes related to different stages of PGC development. Flow cytometry and immunocytochemical staining confirmed the presence of Mvh protein in a small number of cells. There were significant differences between cells that differentiated into PGCs in the group treated with Bmp4 compared to non-treated cells. These findings indicate that ntESCs can differentiate into putative PGCs. Improvement of ntESC differentiation into PGCs may be a reliable means of producing mature germ cells.

Cell Biology International, 2008

In this study characterization of endothelial cells differentiated from human bone marrow mesench... more In this study characterization of endothelial cells differentiated from human bone marrow mesenchymal stem cells (hBMCs) was investigated in relation to their capillary network formation potential. Differentiation was performed in presence of vascular endothelial growth factor (VEGF) and insulin like growth factor-1 (IGF-1). A panel of cellular and molecular markers was used for characterization of the endothelial cells. The cells were strongly positive for von Willebrand factor (vWF) and vascular endothelial growth factor receptor 2 (VEGFR2) when measured at protein and mRNA levels. Development of endothelial cells was found to be associated with formation of typical organelles such as Weibel Palade (WP) bodies, Cavealae and pinocytic vesicles. Early vessel growth was also evidenced by showing specific junctions between the cells. The migratory and angiogenic properties of the cells were confirmed by showing capillary network formation in vitro. These results indicate that the capacity of endothelial cells differentiated from hBMSCs in formation of vascular system is consistent with molecular and structural development.

Iranian journal of reproductive medicine, 2014

Tubal ectopic pregnancy (tEP) is the most common type of extra-uterine pregnancy and the most com... more Tubal ectopic pregnancy (tEP) is the most common type of extra-uterine pregnancy and the most common cause of maternal mortality. Nitric oxide (NO) is a molecule that incorporates in many physiological processes of female reproductive system. Recent studies have demonstrated the possible role of endothelial isoform of nitric oxide synthase (eNOS) enzyme in the regulation of many reproductive events that occur in the fallopian tube (FT). The aim of this study was to evaluate the expression of eNOS in the FTs of women with tEP. In this case-control study, a total number of 30FTs samples were obtained from three groups including: 10 FTs of women that bearing an EP, 10 FTs from the non-pregnant women at luteal phase of the menstrual cycle, and 10 FTs of healthy pregnant women (n=10). Samples were fixed in 10% buffered formalin and then were evaluated by immunohistochemistry. Localization of eNOS was seen in secretory and ciliated luminal epithelium and vascular endothelium of all groups...

Medical science monitor : international medical journal of experimental and clinical research, 2006

The aim of this study was to clarify the histological, ultrastructural and biomechanical effects ... more The aim of this study was to clarify the histological, ultrastructural and biomechanical effects of pentoxifylline (PTX) on the survival of random skin flaps (RSFs) in rats. Thirty male rats were randomly divided into experimental, sham and control groups. The experimental group received PTX 20 mg/kg/day, and the sham group received saline. A 20x70-mm RSF was made 30 days after the commencement of treatment for the three groups. PTX and saline were continued postoperatively for 7 days in the experimental and sham groups, respectively. On the seventh postoperative day, the surviving parts of the flaps were determined and examined through light and transmission electron microscopes. The wounds (incisions) on the margins of the flaps were evaluated histologically and biomechanically. Analysis of variance showed that, in the experimental group, the mean of the surviving parts of the RSFs, fibroblast proliferation, collagen organization and granulation tissue of the wounds was significan...

International Journal of Endocrinology and Metabolism, 2014

Laboratory animal research, 2015

The effect of pentoxifylline (PTX) administration on histomorphological parameters of streptozoto... more The effect of pentoxifylline (PTX) administration on histomorphological parameters of streptozotocin (STZ)-induced type 1 diabetes mellitus (DM) in male rat testes were evaluated. We randomly divided 40 male rats into the following four groups: group 1: control or normal glycemic (NG) rats; group 2 or NG rats that received only normal saline (NS), (NG+NS); group 3 or diabetic rats which were not treated by PTX (DM+vehicle solution (NS)); and group 4 which comprised diabetic rats treated with 50 mg/kg of PTX (DM+PTX). Type 1 DM was induced by intraperitoneal injection of STZ (55 mg/kg). Rats were held for 30 days after which the experimental group received PTX twice daily (25 mg/kg) or NS. After 14 days of treatment by PTX or NS, the left testes from all rats were extracted and prpared for histological study. Apoptotic cells, blood vessel density, and spermatogenesis were evaluated. Data were analyzed by ANOVA test. PTX-treated-diabetic rats showed a significant decrease in number of...

Iranian biomedical journal, 2013

Spinal cord has a limited capacity to repair; therefore, medical interventions are necessary for ... more Spinal cord has a limited capacity to repair; therefore, medical interventions are necessary for treatment of injuries. Transplantation of Schwann cells has shown a great promising result for spinal cord injury (SCI). However, harvesting Schwann cell has been limited due to donor morbidity and limited expansion capacity. Furthermore, accessible sources such as bone marrow stem cells have drawn attentions to themselves. Therefore, this study was designed to evaluate the effect of bone marrow-derived Schwann cell on functional recovery in adult rats after injury. Mesenchymal stem cells were cultured from adult rats' bone marrow and induced into Schwann cells in vitro. Differentiation was confirmed by immunocytochemistry and RT-PCR. Next, Schwann cells were seeded into collagen scaffolds and engrafted in 3 mm lateral hemisection defects. For 8 weeks, motor and sensory improvements were assessed by open field locomotor scale, narrow beam, and tail flick tests. Afterwards, lesioned s...

ABSTRACT Liver tissue engineering with stem cell-derived hepatocyte-like cells provides a promisi... more ABSTRACT Liver tissue engineering with stem cell-derived hepatocyte-like cells provides a promising alternative to liver transplantation in patients with acute and chronic hepatic failure. A serious shortage of liver donors, high cost, the need for immunosuppressive medications and the high risk of transplant rejection remain the major limitations in the _eld of liver transplantation. Using cells instead of organs in this setting should permit (i) expansion of cells in an in vitro phase, (ii) genetic or immunological manipulation of cells for transplantation, (iii) tissue typing and cryopreservation in a cell bank and (iv) the ex vivo genetic modi_cation of the patient’s own cells prior to re-implantation. The creation of an unlimited source of donor cells for hepatocyte transplantation therapy, liver tissue engineering and pharmaceutical applications may be the result of isolation and expansion of progenitor cells or stem cells. The cells are required to function in the same manner as normal hepatic cells, or differentiate into hepatocyte-like cells that can replace the function of the failed liver. The hepatogenic potential of many types of stem cells from different sources has been previously described and the majority of them used for this purpose. The clinical impact of tissue engineering depends upon our ability to direct cells to form tissues with characteristic structural and mechanical properties from the molecular level up to organized tissue. In most cases, tissue engineering attempts to recapitulate certain aspects of normal development in order to stimulate cell differentiation and functional tissue assembly. The induction of tissue growth generally involves the use of biodegradable and bioactive materials designed, ideally, to provide a mechanical, physical and biochemical template for tissue regeneration. Function and differentiation of liver cells and stem cell-derived hepatocyte-like cells are in_uenced by the three-dimensional organ architecture. Hepatocytes are attachment-dependent cells and lose their liver-speci_c functions or die without an optimal normal microenvironment, extracellular matrix composition and cell-cell contacts. The use of polymeric matrices and/or nano_brillar components permits the three-dimensional formation of a neo tissue and speci_c stimulation by adequate modi_cation of the matrix surface, which might be essential for appropriate differentiation of transplanted cells. In addition to development of bioarti_cial liver devices (BAL) for extracorporeal liver support and intracorporeal liver replacement, a concept that combines tissue engineering using three-dimensional, highly porous matrices with cell transplantation could be useful. However, the creation and development of a functional liver tissue for transplantation must overcome the limited distance of oxygen diffusion and accessibility to blood plasma. Therefore induction and creation of efcient vascular networks has been one of the fundamental challenges of liver tissue engineering either in vitro, for the transplantation of prevascularized constructs, or in vivo, for cellular organization and transplant incorporation within the implantation site. Vascularization in vitro could maintain cell viability during tissue growth, induce structural organization and promote vascularization into a host tissue upon implantation. In summary, as the liver is a very complex organ and has dual functions of both metabolism and detoxication through incorporation of heterotypic cell-cell interactions, 3D architecture and perfused flow, it is crucial to consider the choice of cell source and culture condition, type of scaffolds and creation of vascular networks for long-term maintenance of tissue-specic functions required to reconstruct a tissue-engineered liver.

ABSTRACT Background: Pentoxifylline decreased blood viscosity, improved peripheral blood circulat... more ABSTRACT Background: Pentoxifylline decreased blood viscosity, improved peripheral blood circulation, and improved red blood cell flexibility. It also induced sperm motility and increased microvascular blood circulation in non-diabetic humans. The aim of present study was to evaluate the effect of pentoxifylline administration on Sertoli and Leydig cells count of experimentally induced type 1 diabetes in male rats. Materials and methods: In this experimental study 50 male rats were randomly divided into 3 nondiabetic groups, and 2 diabetic groups. Type 1 diabetes was induced by injection of streptozotocin, and rats were held for 30 days. Experimental groups on non-diabetic and diabetic rats were received pentoxifylline daily. One non-diabetic group received daily normal saline. Then, all rats were anesthetized and their right testis were extracted and were examined histologically. Sertoli cells and Leydig cells were counted. Data were analyzed by ANOVA and LSD tests. Results: Increase in number of pentoxifylline- treated diabetic rats cells (7.43±0.86, 15.25±1.6) were significantly higher than control diabetic rats (4.4±0.38, 11±1.31) (p=0.001 and p=0.000 respectively). There were significant difference between pentoxifylline treated diabetic rats cells (7.43±0.86, 15.25±1.6), and normal saline- treated non- diabetic rats cells (7.17±1.13, 25.8, 3.5) with other groups (p=0.001 and p=0.000 respectively). There were no significant difference between non- diabetic groups. Conclusion: Our results showed that pentoxifylline administration increased Sertoli cells and Leydig cells count in non-diabetic and diabetic rats in compare to control groups. However its effect in diabetic rats were significant. Keywords: Diabetes Mellitus, Pentoxifylline, Sertoli Cells, Leydig Cells, Rat

ABSTRACT When the spinal cord is damaged, medical procedures are vital to prevent of improvement ... more ABSTRACT When the spinal cord is damaged, medical procedures are vital to prevent of improvement of the lesion. Because of poor regeneration ability of central nervous tissue, the most injuries are irreversible. One of encouraging interventions for treatment of spinal cord injury is Schwann cell transplantation. However, isolation of Schwann cell for clinical interventions is complicated approach with low cells yield and purity. Thus, easily accessed sources like Adipose mesenchymal stem cells have been taken notice. Therefore, this study was planned to assess the effect of adipose stromal cell-derived Schwann cell transplantation in functional recovery after lateral hemisection in adult rats. After isolation, adipose stem cells were differentiated to Schwann cells. The differentiation was verified by immunocytochemistry and reverse transcription-polymerase chain reaction (RT-PCR). Then, we loaded the cells into collagen scaffolds with parallel aligned canals and transplanted into rats with 3 mm lesions at T9 - T10 level. Motor and sensory improvement were evaluated by open field locomotor scale, narrow beam, and tail flick tests for 60 days. Subsequently, conventional histology and immunohistochemistry were performed. In vitro results revealed that mesenchymal stem cells after differentiation gained Schwann cells morphology and markers. Schwann cell-grafted group had significantly higher locomotor and sensory scores in comparison with the control and scaffold without cell groups. Histological observations showed differentiated cells have the ability to improve axonal regeneration and remyelination. Our study proved that adipose tissue- derived Schwann cells can change the rough environment of damaged spinal cord and support axon regeneration and enhance functional recovery, and possibly be helpful for people suffering from spinal cord injuries.

Biomaterials, 2009

The interactions of bone marrow-derived mesenchymal stem cells (MSCs) and their engrafted microen... more The interactions of bone marrow-derived mesenchymal stem cells (MSCs) and their engrafted microenvironment are an integral part of signaling control of stem cell lineage commitment. We attempted to induce bone marrow-derived MSCs to undergo epidermal lineage differentiation by manipulating the biochemical, environmental and physical properties of culture conditions in an organotypic coculture model to simulate a skin-specific microenvironment. The induction medium was optimized by varying different biomolecular supplements in a basic stratification medium. A multi-layered epidermis-like structure was established when MSCs were cultured in an optimized induction medium on a contractible fibroblast-embedded collagen gel with an air-liquid interface. The commitment into epidermal lineage was further confirmed by the expression of early and intermediate epidermalization markers - keratin 10 and filaggrin in 90.67% and 80.51% of MSCs, respectively. This study not only highlights the possibility of in vitro control of MSCs into epidermal lineage, but also suggests the therapeutic potential of bone marrow-derived MSCs for skin regeneration.

Genetics and Molecular Biology, 2015

Nuclear transfer embryonic stem cells (ntESCs) show stem cell characteristics such as pluripotenc... more Nuclear transfer embryonic stem cells (ntESCs) show stem cell characteristics such as pluripotency but cause no immunological disorders. Although ntESCs are able to differentiate into somatic cells, the ability of ntESCs to differentiate into primordial germ cells (PGCs) has not been examined. In this work, we examined the capacity of mouse ntESCs to differentiate into PGCs in vitro. ntESCs aggregated to form embryoid bodies (EB) in EB culture medium supplemented with bone morphogenetic protein 4(BMP4) as the differentiation factor. The expression level of specific PGC genes was compared at days 4 and 8 using real time PCR. Flow cytometry and immunocytochemical staining were used to detect Mvh as a specific PGC marker. ntESCs expressed particular genes related to different stages of PGC development. Flow cytometry and immunocytochemical staining confirmed the presence of Mvh protein in a small number of cells. There were significant differences between cells that differentiated into PGCs in the group treated with Bmp4 compared to non-treated cells. These findings indicate that ntESCs can differentiate into putative PGCs. Improvement of ntESC differentiation into PGCs may be a reliable means of producing mature germ cells.

Cell Biology International, 2008

In this study characterization of endothelial cells differentiated from human bone marrow mesench... more In this study characterization of endothelial cells differentiated from human bone marrow mesenchymal stem cells (hBMCs) was investigated in relation to their capillary network formation potential. Differentiation was performed in presence of vascular endothelial growth factor (VEGF) and insulin like growth factor-1 (IGF-1). A panel of cellular and molecular markers was used for characterization of the endothelial cells. The cells were strongly positive for von Willebrand factor (vWF) and vascular endothelial growth factor receptor 2 (VEGFR2) when measured at protein and mRNA levels. Development of endothelial cells was found to be associated with formation of typical organelles such as Weibel Palade (WP) bodies, Cavealae and pinocytic vesicles. Early vessel growth was also evidenced by showing specific junctions between the cells. The migratory and angiogenic properties of the cells were confirmed by showing capillary network formation in vitro. These results indicate that the capacity of endothelial cells differentiated from hBMSCs in formation of vascular system is consistent with molecular and structural development.

Iranian journal of reproductive medicine, 2014

Tubal ectopic pregnancy (tEP) is the most common type of extra-uterine pregnancy and the most com... more Tubal ectopic pregnancy (tEP) is the most common type of extra-uterine pregnancy and the most common cause of maternal mortality. Nitric oxide (NO) is a molecule that incorporates in many physiological processes of female reproductive system. Recent studies have demonstrated the possible role of endothelial isoform of nitric oxide synthase (eNOS) enzyme in the regulation of many reproductive events that occur in the fallopian tube (FT). The aim of this study was to evaluate the expression of eNOS in the FTs of women with tEP. In this case-control study, a total number of 30FTs samples were obtained from three groups including: 10 FTs of women that bearing an EP, 10 FTs from the non-pregnant women at luteal phase of the menstrual cycle, and 10 FTs of healthy pregnant women (n=10). Samples were fixed in 10% buffered formalin and then were evaluated by immunohistochemistry. Localization of eNOS was seen in secretory and ciliated luminal epithelium and vascular endothelium of all groups...

Medical science monitor : international medical journal of experimental and clinical research, 2006

The aim of this study was to clarify the histological, ultrastructural and biomechanical effects ... more The aim of this study was to clarify the histological, ultrastructural and biomechanical effects of pentoxifylline (PTX) on the survival of random skin flaps (RSFs) in rats. Thirty male rats were randomly divided into experimental, sham and control groups. The experimental group received PTX 20 mg/kg/day, and the sham group received saline. A 20x70-mm RSF was made 30 days after the commencement of treatment for the three groups. PTX and saline were continued postoperatively for 7 days in the experimental and sham groups, respectively. On the seventh postoperative day, the surviving parts of the flaps were determined and examined through light and transmission electron microscopes. The wounds (incisions) on the margins of the flaps were evaluated histologically and biomechanically. Analysis of variance showed that, in the experimental group, the mean of the surviving parts of the RSFs, fibroblast proliferation, collagen organization and granulation tissue of the wounds was significan...

International Journal of Endocrinology and Metabolism, 2014

Oligoasthenoteratozoospermia (OAT) is demonstrated to be one of the most common causes of male su... more Oligoasthenoteratozoospermia (OAT) is demonstrated to be one of the most common causes of male subfertility. Phospholipase C ζ (PLCζ), a sperm-specific protein, is considered to be one of the sperm-borne oocyte activating factors (SOAFs), which play a vital role in fertilization. The post-acrosomal sheath WW domain-binding protein (PAWP) is another candidate for SOAF. The aim of this study was to compare the PLCζ localization patterns and percentage of PLCζ- and PAWP-positive sperm cells in patients with OAT and fertile men with normozoospermia. A total of 40 men included in this study were classified into two groups: OAT (n = 25) and control group (n = 15). Semen samples were collected and analyzed using conventional semen analysis according to the World Health Organization guidelines. The percentage of PLCζ- and PAWP-positive sperm cells and localization patterns of PLCζ were evaluated using immunofluorescence staining. The mean percentage of sperm cells expressing PAWP and PLCζ was significantly lower in OAT compared to control group (52.8 ± 4.2 vs. 76.8 ± 5 and 63.4 ± 3.5 vs. 86.7 ± 2.1, respectively). In addition, statistically significant differences were found with regard to the PLCζ localization patterns, including equatorial, acrosomal + equatorial, and equatorial + post-acrosomal pattern, between the two groups (p < 0.01). The present study showed a lower percentage of sperm cells expressing PLCζ and PAWP, as well as altered localization patterns of PLCζ in men with OAT. Given the role of PLCζ and PAWP in fertilization, as two major candidates for SOAFs, our findings indicate that PLCζ and PAWP impairments may be one of the possible etiologies of decreased fertility in OAT.