javad ranjbari | Shahid Beheshti University of Medical Sciences (original) (raw)

Papers by javad ranjbari

Iranian Journal of Pharmaceutical Research : IJPR, 2021

Nowadays, mesenchymal stem cells (MSCs) are the most widely used cell sources for bone regenerati... more Nowadays, mesenchymal stem cells (MSCs) are the most widely used cell sources for bone regenerative medicine. Electrospun polyacrylonitrile (PAN)-based scaffolds play an important role in bone tissue engineering due to their good mechanical properties, which could be enhanced by the presence of nanoparticles such as nanoclay. This study evaluated the in-vitro effect of different concentrations of nanoclay in surface characteristic properties of PAN-based electrospun nanofiber scaffolds and the osteogenic differentiation ability of adipose-derived mesenchymal stem cells (AD-MSCs). After electrospinning nanofibers, their structure were assessed through some characterization tests. Then AD-MSCs isolation and characterization were done, and the cell attachment and the biocompatibility were determined. Finally, osteogenic differentiation-related markers, genes, and proteins were studied. Clay-PAN25% electrospun nanofiber scaffold could support attachment, proliferation, and osteogenic di...

Current Stem Cell Research & Therapy, 2020

: It has been almost 18 months since the first outbreak of COVID-19 disease was reported in Wuhan... more : It has been almost 18 months since the first outbreak of COVID-19 disease was reported in Wuhan, China. This unexpected devastating phenomenon, raised a great deal of concerns and anxiety among people around the world and imposed a huge economic burden on the nations’ health care systems. Accordingly, clinical scientists, pharmacologists and physicians worldwide felt an urgent demand for a safe, effective therapeutic agent, treatment strategy or vaccine in order to prevent or cure the recently-emerged disease. Initially, due to the lack of specific pharmacological agents and approved vaccines to combat the COVID-19, the disease control in the confirmed cases was limited to supportive care. Accordingly, repositioning or repurposing current drugs and examining their possible therapeutic efficacy received a great deal of attention. Despite revealing promising results in some clinical trials, the overall results are conflicting. For this reason, there is an urgent need to seek and investigate other potential therapeutics. Mesenchymal stem cells (MSC), representing immunomodulatory and regenerative capacity to treat both curable and intractable diseases, have been investigated in COVID-19 clinical trials carried out in different parts of the world. Nevertheless, up to now, none of the MSC-based approaches has been approved in controlling COVID-19 infection. Thanks to the fact that the final solution for defeating the pandemic is developing a safe, effective vaccine, enormous efforts and clinical research have been carried out. In this review, we will concisely discuss the safety and efficacy of the most relevant pharmacological agents, MSC-based approaches and candidate vaccines for treating and preventing COVID-19 infection.

Iranian Journal of Virology, Dec 1, 2008

Letters in Drug Design & Discovery

Background: Apoptosis of brain cells (neurons and glia) has a crucial role in the pathology of tr... more Background: Apoptosis of brain cells (neurons and glia) has a crucial role in the pathology of traumatic brain injury (TBI) in humans. So, a decrease in the apoptosis rate can potentially reduce the harmful effects and lead to better functional outcomes. Drug repurposing by computational methodologies like protein-ligand docking allows us to make drug discovery more efficient and less expensive. Objective: In the current study we used the methodology to study the inhibitory effect of thousands of FDA/non-FDA approved, investigational compounds on caspase 3 as one of the most important members of the cell apoptosis pathway. Methods: Molecular docking and pharmacokinetic properties calculations were done. The molecular dynamics (MD) simulations of all complexes and free caspase 3 were carried out. We carried out docking experiments using in silico methods and docked a pool of medications to the active site of the human caspase-3 X-ray structure. The best compounds were selected and su...

BioImpacts, 2021

Introduction: Treatment of critical-sized bone defects is challenging. Tissue engineering as a st... more Introduction: Treatment of critical-sized bone defects is challenging. Tissue engineering as a state-of-the-art method has been concerned with treating these non-self-healing bone defects. Here, we studied the potentials of new three-dimensional nanofibrous scaffolds (3DNS) with and without human adipose mesenchymal stem cells (ADSCs) for reconstructing rat critical-sized calvarial defects (CSCD). Methods: Scaffolds were made from 1- polytetrafluoroethylene (PTFE), and polyvinyl alcohol (PVA) (PTFE/ PVA group), and 2- PTFE, PVA, and graphene oxide (GO) nanoparticle (PTFE/ PVA/GO group) and seeded by ADSCs and incubated in osteogenic media (OM). The expression of key osteogenic proteins including Runt-related transcription factor 2 (Runx2), collagen type Iα (COL Iα), osteocalcin (OCN), and osteonectin (ON) at days 14 and 21 of culture were evaluated by western blot and immunocytochemistry methods. Next, 40 selected rats were assigned to five groups (n=8) to create CSCD which will be ...

Iranian journal of pharmaceutical research, 2015

Human insulin-like growth factor I (hIGF-I) is a kind of growth factor with clinical signiﬁcance ... more Human insulin-like growth factor I (hIGF-I) is a kind of growth factor with clinical signiﬁcance in medicine. Up to now, E. coli expression system has been widely used as a host to produce rhIGF-1 with high yields. Batch cultures as non-continuous fermentations were carried out to overproduce rhIGF-I in E. coli. The major objective of this study is over- production of recombinant human insulin-like growth factor I (rhIGF-I) through a developed process by recruiting effective factors in order to achieve the most recombinant protein. In this study we investigated the effect of culture medium, induction temperature and amount of inducer on cell growth and IGF-1 production. Taguchi design of experiments (DOE) method was used as the statistical method. Analysis of experimental data showed that maximum production of rhIGF-I was occurred in 32y culture medium at 32 °C and 0.05 Mm IPTG. Under this condition, 0.694 g/L of rhIGF-I was produced as the inclusion bodies. Following optimization o...

Pharmaceutical Sciences, 2010

Selection of a suitable host strain is an important step in recombinant production of heterologou... more Selection of a suitable host strain is an important step in recombinant production of heterologous proteins. As the prokaryotic systems and especially Escherichia coli (E. coli) cells are the most attractive host in therapeutic protein production, various derivatives of this organism have been developed to overcome the limitations exist in recombinant protein production, such as inefficient expression and folding of target proteins with eukaryotic origin. This review summarized key strategies for E. coli engineering and introduced some new and applicable engineered E. coli strains that produce more complex proteins for therapeutic and research use in the future. HIGHLIGHTS • Selection of a suitable host is an important step in recombinant protein production. • Escherichia coli cells are the most attractive host in therapeutic protein production. • Engineered strains had developed to produce active protein with suitable conformation in industrial scale. • Classical mutagenesis & geno...

Zahedan Journal of Research in Medical Sciences, 2014

Background: The purpose of this study is to evaluate the effect of Curcuma longa extract on the t... more Background: The purpose of this study is to evaluate the effect of Curcuma longa extract on the telomerase gene expression in QU-DB lung cancer and T47D breast cancer cell lines. Materials and Methods: The present study is an experimental research. Using 3 different phases n-hexane, dichloromethane and methanol, total extract of Curcuma longa in a serial dilution was prepared and three phases was analyzed for determining which phase has more curcuminoids. Then the extract cytotoxicity effect was tested on breast cancer cell line (T47D), and lung cancer cell line (QU-DB) by 24, 48 and 72 h MTT (Dimethyl thiazolyl diphenyl tetrazolium) assay. Then, the cells were treated with serial concentrations of the extract. Finally, total protein was extracted from the control and test groups, its quantity was determined and telomeric repeat amplification protocol (TRAP) assay was performed for measurement of possible inhibition of the telomerase activity. Results: Cell viability and MTT-based cytotoxicity assay show that the total extract of Curcuma longa has cytotoxic effect with different IC50s in breast and lung cancer cell lines. Analysis of TRAP assay also shows a significant reduction in telomerase activity on both cancer cells with different levels. Conclusion: Curcuma longa extract has anti-proliferation and telomerase inhibitory effects on QU-DB lung cancer and T47D breast cancer cells with differences in levels of telomerase inhibition.

Insulin-Like Growth Factor-1 (IGF-1) is a small peptide with 70 amino acids and 7.6 kDa molecular... more Insulin-Like Growth Factor-1 (IGF-1) is a small peptide with 70 amino acids and 7.6 kDa molecular weight that acts as the major mediator of growth hormone. According to the previous studies, recombinant production of human IGF-1 (rhIGF-1) in E. coli has resulted in an inactive form of protein (inclusion body). There are several strategies to transform inclusion body to a soluble form. Production in the form of fusion proteins as a suitable strategy, helps researcher in recombinant production of proteins in the soluble and active form. In current study, NusA fusion protein was used to produce IGF-1 soluble form, instead of insoluble protein. In previous study, rhIGF-1 was optimally expressed in inclusion body with 1.2 g/L concentration. rhIGF1 -NusA construct was cloned and expressed in E. coli, then, cell lysate was analyze by SDS-PAGE and densitometry techniques, to assay soluble and insoluble form of rhIGF-1. Results showed that rhIGF-1 concentration in soluble phase was 0.14 g/L,...

Iranian Journal of Pharmaceutical Research : IJPR, 2019

Researchers add serum to a classical medium at concentrations of 5 to 10% (v/v) to grow cells in-... more Researchers add serum to a classical medium at concentrations of 5 to 10% (v/v) to grow cells in-vitro culture media. Unfortunately, serum is a poorly defined culture medium component as its composition can vary considerably while serum-free cell culture media are an excellent alternative to standard serum-containing media and offer several major advantages. Advantages of using serum-free media include a lower risk of infectious agents, lower risk of interfering components, less contaminant, avoids ethical issues. According to previous studies insulin, selenium, transferrin and glucose are important component of serum that affect cell growth. In the present study, we optimized amount of these factors in order to serum free culture medium fabrication. Response surface methodology (RSM) was employed for optimization of key factors in serum free medium to enhance recombinant human GM-CSF (rhGM-CSF) production in CHO cell line. Four important process parameters including insulin concent...

Protein-coated nanoparticles have diverse applications in biomedical science. The protein hydroph... more Protein-coated nanoparticles have diverse applications in biomedical science. The protein hydrophobic domains or surface electrostatic charge conducts adsorption of proteins to different surfaces. This property can be customized to immobilize specific molecules on solid supports for experimental screenings or purification processes. To develop highly selective affinity ligands—such as aptamers—against specific protein targets, protein-coated magnetic particles have been successfully applied. This approach could be highly efficient in affinity ligand development against coagulation factor VIII.In this study, magnetic nanoparticles were prepared by co-precipitation method and, then, a gold coating was run on the MNPs’ surface. The gold coating could add some attractive specifications to the protein immobilized nanoparticles during the aptamer selection process, such as simultaneous affinity determination of aptameric oligonucleotides by fluorescence-based methods. The gold surface has...

Iranian Journal of Biotechnology, 2021

Background: Acetate accumulation in the culture medium is known as an inhibitor in recombinant pr... more Background: Acetate accumulation in the culture medium is known as an inhibitor in recombinant protein production in Escherichia coli. Various approaches have been proposed and evaluated to overcome this challenge and reduce the concentration of acetate. In this study, we examined the effect of acetate kinase A antisense on acetate production rate in E. coli We also used PAMAM dendrimers as a suitable delivery agent for antisense transformation into E. coli host cell. Objective: This study aimed to decrease acetate production as a by-product using an antisense-dendrimer complex to increase mass cell and subsequently recombinant Albumin production in E. coli. Materials and Methods: Here, to study the effect of this treatment on recombinant protein production, we used pET22b/HAS construct. The ackA gene expression was inhibited by designed antisense to reduce acetate concentration in culture medium. AckA antisense was transferred to E. coli by PAMAM dendrimer. Finally, ackA expression...

African Journal of …, 2010

African Journal of Biotechnology Vol. 9 (6), pp. 912-919, 8 February, 2010 Available online at ht... more African Journal of Biotechnology Vol. 9 (6), pp. 912-919, 8 February, 2010 Available online at http://www.academicjournals.org/AJB ISSN 16845315 © 2010 Academic Journals ... The inhibitory effect of Curcuma longa extract on ... Pourhassan Mohammad, Zarghami Nosratollah*, ...

BioImpacts, Feb 8, 2020

Introduction: Simulating hydrophobic-hydrophilic composite face with hierarchical porous and fibr... more Introduction: Simulating hydrophobic-hydrophilic composite face with hierarchical porous and fibrous architectures of bone extracellular matrix (ECM) is a key aspect in bone tissue engineering. This study focused on the fabrication of new three-dimensional (3D) scaffolds containing polytetrafluoroethylene (PTFE), and polyvinyl alcohol (PVA), with and without graphene oxide (GO) nanoparticles using the chemical cross-linking and freeze-drying methods for bone tissue application. The effects of GO on physicochemical features and osteoinduction properties of the scaffolds were evaluated through an in vitro study. Methods: After synthesizing the GO nanoparticles, two types of 3D scaffolds, PTFE/PVA (PP) and PTFE/PVA/GO (PPG), were developed by cross-linking and freeze-drying methods. The physicochemical features of scaffolds were assessed and the interaction of the 3D scaffold types with human adipose mesenchymal stem cells (hADSCs) including attachment, proliferation, and differentiation to osteogenic like cells were investigated. Results: GO nanoparticles were successfully synthesized with no agglomeration. The blending of PTFE as a hydrophobic polymer with PVA polymer and GO nanoparticles (hydrophilic compartments) were successful. Two types of 3D scaffolds had nano topographical structures, good porosities, hydrophilic surfaces, thermal stabilities, good stiffness, as well as supporting the cell attachments, proliferation, and osteogenic differentiation. Notably, GO incorporating scaffolds provided a better milieu for cell behaviors. Conclusion: Novel multiscale porous nanofibrous 3D scaffolds made from PTFE/ PVA polymers with and without GO nanoparticles could be an ideal candidate for bone tissue engineering as a 3D template.

Journal of Drug Delivery Science and Technology

Current Stem Cell Research & Therapy

: Currently, combining stem cells [SCs] with biomaterial scaffolds provides a promising strategy ... more : Currently, combining stem cells [SCs] with biomaterial scaffolds provides a promising strategy for the future of biomedicine and regenerative medicine [RG]. The cells need similar substrates of extra cellular matrix [ECM] for normal tissue development that signifies the importance of three dimensional [3D] scaffolds to determine cell fate. Herein, we present the great importance and also positive contributions of corresponding 3D scaffolds on cell functions including cell interactions, cell migrations, and nutrient delivery. Further on, the synthesis techniques which are recruited to fabricate the 3D scaffolds, are discussed besides the related studies of 3D scaffold for different tissues are reported in this paper completely. This review focuses on 3D scaffolds that have been used for tissue engineering purposes and directing stem cell fate as a means of producing replacements for biomedical applications.