J. Kirz | SUNY: Stony Brook University (original) (raw)
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Papers by J. Kirz
Proceedings of the National Academy of Sciences, 2005
We have used the method of x-ray diffraction microscopy to image the complex-valued exit wave of ... more We have used the method of x-ray diffraction microscopy to image the complex-valued exit wave of an intact and unstained yeast cell. The images of the freeze-dried cell, obtained by using 750-eV x-rays from different angular orientations, portray several of the cell's major internal components to 30-nm resolution. The good agreement among the independently recovered structures demonstrates the accuracy of the imaging technique. To obtain the best possible reconstructions, we have implemented procedures for handling noisy and incomplete diffraction data, and we propose a method for determining the reconstructed resolution. This work represents a previously uncharacterized application of x-ray diffraction microscopy to a specimen of this complexity and provides confidence in the feasibility of the ultimate goal of imaging biological specimens at 10-nm resolution in three dimensions. coherent x-ray diffraction imaging ͉ x-ray microscopy X -ray diffraction microscopy is a recently developed method in which only the coherent diffraction pattern of the sample is measured. It provides a path to high resolution without the limitations imposed by an x-ray optical system. The idea to image a noncrystalline object by phasing and inverting its diffraction pattern goes back to a suggestion by Sayre (1, 2) and was first demonstrated with x-rays by Miao et al. (3). In this article, we report the imaging of the complex-valued exit wavefront (both phase and magnitude) of a whole freeze-dried and unstained yeast cell. The images, at 30-nm resolution from multiple angular orientations of the cell, required an exposure of approximately one minute each using 750-eV x-rays (1 eV ϭ 1.602 ϫ 10 Ϫ19 J). This demonstration paves the way for the application of 3D x-ray diffraction microscopy (XDM) (4, 5) to frozen-hydrated samples in the future.
Physical Review Letters, 1976
ABSTRACT
Photochemistry and Photobiology, 1986
ABSTRACT
Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 1994
Inner shell processes in the light elements provide the contrast mechanisms for soft X-ray micros... more Inner shell processes in the light elements provide the contrast mechanisms for soft X-ray microscopy. Bright X-ray sources and precision focusing elements have led to the development of high resolution instruments. Our efforts in scanning microscopy, holography and diffraction at the National Synchrotron Light Source are aimed at the development of microimaging techniques mostly for biological specimens.
IEEE Transactions on Nuclear Science, 2000
A ring-imaging Cherenkov detector has been used to separate 7r, K-, and antiprotons in a 200 GeV/... more A ring-imaging Cherenkov detector has been used to separate 7r, K-, and antiprotons in a 200 GeV/c test beam at Fermilab. The photon detector was a multistep avalanche chamberl with a proportional wire chamber as second stage. Cherenkov photons near 8 eV were ...
Proceedings of …, 1995
The Stony Brook scanning transmission x-ray microscope (STXM) has been operating at the X1A beaml... more The Stony Brook scanning transmission x-ray microscope (STXM) has been operating at the X1A beamline at the NSLS since 1989. A large number of users have used it to study biological and material science samples. We report on changes that have been performed in the ...
Proceedings of the National Academy of Sciences, 2005
We have used the method of x-ray diffraction microscopy to image the complex-valued exit wave of ... more We have used the method of x-ray diffraction microscopy to image the complex-valued exit wave of an intact and unstained yeast cell. The images of the freeze-dried cell, obtained by using 750-eV x-rays from different angular orientations, portray several of the cell's major internal components to 30-nm resolution. The good agreement among the independently recovered structures demonstrates the accuracy of the imaging technique. To obtain the best possible reconstructions, we have implemented procedures for handling noisy and incomplete diffraction data, and we propose a method for determining the reconstructed resolution. This work represents a previously uncharacterized application of x-ray diffraction microscopy to a specimen of this complexity and provides confidence in the feasibility of the ultimate goal of imaging biological specimens at 10-nm resolution in three dimensions. coherent x-ray diffraction imaging ͉ x-ray microscopy X -ray diffraction microscopy is a recently developed method in which only the coherent diffraction pattern of the sample is measured. It provides a path to high resolution without the limitations imposed by an x-ray optical system. The idea to image a noncrystalline object by phasing and inverting its diffraction pattern goes back to a suggestion by Sayre (1, 2) and was first demonstrated with x-rays by Miao et al. (3). In this article, we report the imaging of the complex-valued exit wavefront (both phase and magnitude) of a whole freeze-dried and unstained yeast cell. The images, at 30-nm resolution from multiple angular orientations of the cell, required an exposure of approximately one minute each using 750-eV x-rays (1 eV ϭ 1.602 ϫ 10 Ϫ19 J). This demonstration paves the way for the application of 3D x-ray diffraction microscopy (XDM) (4, 5) to frozen-hydrated samples in the future.
Physical Review Letters, 1976
ABSTRACT
Photochemistry and Photobiology, 1986
ABSTRACT
Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 1994
Inner shell processes in the light elements provide the contrast mechanisms for soft X-ray micros... more Inner shell processes in the light elements provide the contrast mechanisms for soft X-ray microscopy. Bright X-ray sources and precision focusing elements have led to the development of high resolution instruments. Our efforts in scanning microscopy, holography and diffraction at the National Synchrotron Light Source are aimed at the development of microimaging techniques mostly for biological specimens.
IEEE Transactions on Nuclear Science, 2000
A ring-imaging Cherenkov detector has been used to separate 7r, K-, and antiprotons in a 200 GeV/... more A ring-imaging Cherenkov detector has been used to separate 7r, K-, and antiprotons in a 200 GeV/c test beam at Fermilab. The photon detector was a multistep avalanche chamberl with a proportional wire chamber as second stage. Cherenkov photons near 8 eV were ...
Proceedings of …, 1995
The Stony Brook scanning transmission x-ray microscope (STXM) has been operating at the X1A beaml... more The Stony Brook scanning transmission x-ray microscope (STXM) has been operating at the X1A beamline at the NSLS since 1989. A large number of users have used it to study biological and material science samples. We report on changes that have been performed in the ...