Expression of ghrelin receptor mRNA in the rat and the mouse brain. (original) (raw)
Searching across hundreds of databases
Our searching services are busy right now. Your search will reload in five seconds.
X
Forgot Password
If you have forgotten your password you can enter your email here and get a temporary password sent to your email.
X
Forgot Password
If you have forgotten your password you can enter your email here and get a temporary password sent to your email.
The Journal of comparative neurology | 2006
Ghrelin is a hormone that stimulates growth hormone secretion and signals energy insufficiency via interaction with its receptor, the growth hormone secretagogue receptor (GHSR). The GHSR is located in both the central nervous system and the periphery. Its distribution in the CNS, as assessed by in situ hybridization histochemistry (ISHH), has been described previously in a few mammalian species, although these studies were limited by either the detail provided or the extent of the regions examined. In the present study, we systematically examined the distribution of GHSR mRNA in the adult rat and mouse brains and cervical spinal cords by using ISHH with novel cRNA probes specific for the mRNA encoding functional GHSR (the type 1a variant). We confirmed GHSR mRNA expression in several hypothalamic nuclei, many of which have long been recognized as playing roles in body weight and food intake. GHSR also was found in several other regions previously unknown to express GHSR mRNA, including many parasympathetic preganglionic neurons. Additionally, we found GHSR mRNA within all three components of the dorsal vagal complex, including the area postrema, the nucleus of the solitary tract, and the dorsal motor nucleus of the vagus. Finally, we examined the coexpression of GHSR with tyrosine hydroxylase and cholecystokinin and demonstrate a high degree of GHSR mRNA expression within dopaminergic, cholecystokinin-containing neurons of the substantia nigra and ventral tegmental area.
Pubmed ID: 16320257 RIS Download
Research resources used in this publication
None found
Antibodies used in this publication
None found
Associated grants
- Agency: NIDDK NIH HHS, United States
Id: R01 DK053301-08S1 - Agency: NIDDK NIH HHS, United States
Id: 5T32DK07516-18 - Agency: NIDDK NIH HHS, United States
Id: R37 DK053301 - Agency: NIDDK NIH HHS, United States
Id: 1F32DK064564-01 - Agency: NIDDK NIH HHS, United States
Id: DK56116 - Agency: NIDDK NIH HHS, United States
Id: R01 DK071320-04 - Agency: NIDDK NIH HHS, United States
Id: F32 DK064564 - Agency: NIDDK NIH HHS, United States
Id: R01 DK053301 - Agency: NIDDK NIH HHS, United States
Id: P01 DK056116 - Agency: NIDDK NIH HHS, United States
Id: DK53301 - Agency: NIDDK NIH HHS, United States
Id: T32 DK007516 - Agency: NIDDK NIH HHS, United States
Id: R01 DK071320