Zebrafish sox9b is crucial for hepatopancreatic duct development and pancreatic endocrine cell regeneration. (original) (raw)
Searching across hundreds of databases
Our searching services are busy right now. Your search will reload in five seconds.
X
Forgot Password
If you have forgotten your password you can enter your email here and get a temporary password sent to your email.
X
Forgot Password
If you have forgotten your password you can enter your email here and get a temporary password sent to your email.
Developmental biology | 2012
Recent zebrafish studies have shown that the late appearing pancreatic endocrine cells are derived from pancreatic ducts but the regulatory factors involved are still largely unknown. Here, we show that the zebrafish sox9b gene is expressed in pancreatic ducts where it labels the pancreatic Notch-responsive cells previously shown to be progenitors. Inactivation of sox9b disturbs duct formation and impairs regeneration of beta cells from these ducts in larvae. sox9b expression in the midtrunk endoderm appears at the junction of the hepatic and ventral pancreatic buds and, by the end of embryogenesis, labels the hepatopancreatic ductal system as well as the intrapancreatic and intrahepatic ducts. Ductal morphogenesis and differentiation are specifically disrupted in sox9b mutants, with the dysmorphic hepatopancreatic ducts containing misdifferentiated hepatocyte-like and pancreatic-like cells. We also show that maintenance of sox9b expression in the extrapancreatic and intrapancreatic ducts requires FGF and Notch activity, respectively, both pathways known to prevent excessive endocrine differentiation in these ducts. Furthermore, beta cell recovery after specific ablation is severely compromised in sox9b mutant larvae. Our data position sox9b as a key player in the generation of secondary endocrine cells deriving from pancreatic ducts in zebrafish.
Pubmed ID: 22537488 RIS Download
Research resources used in this publication
None found
Additional research tools detected in this publication
None found
Associated grants
- Agency: NCI NIH HHS, United States
Id: P30 CA015704 - Agency: NIDDK NIH HHS, United States
Id: DK090816 - Agency: NHGRI NIH HHS, United States
Id: HG002995 - Agency: NHGRI NIH HHS, United States
Id: R01 HG002995 - Agency: NIDDK NIH HHS, United States
Id: R01 DK080730 - Agency: Howard Hughes Medical Institute, United States
- Agency: NIDDK NIH HHS, United States
Id: RC4 DK090816 - Agency: NIDDK NIH HHS, United States
Id: R01 DK064646 - Agency: NIDDK NIH HHS, United States
Id: DK080730 - Agency: NICHD NIH HHS, United States
Id: R01 HD076585