Mitosis-Coupled, Microtubule-Dependent Clustering of Endosomal Vesicles around Centrosomes (original) (raw)

“…In that study, it was demonstrated that phosphorylation of BARS on Ser147 by PAK1-PAK3 induces BARS interaction with the 14-3-3γ dimer and PI4KIIIβ which, together, serve as a Golgi shuttling complex. However, this mechanism was suggested to act specifically in the vesiculization of the Golgi, which is not very active during mitosis (Takatsu et al, 2013). Here, we demonstrate that the PI4KIIIβ-14-3-3γ complex does affect mitotic Golgi translocation of ERK1c.…”

Section: Discussionmentioning

“…In essence, Rab11-endosomes act as carriers to recruit and organize MT-nucleating material at spindle poles through dynein (Figure 1, 2, 3). We believe that this is particularly significant because previous studies suggested that endosomes, like Golgi complexes and endoplasmic reticulum disperse throughout the cytoplasm during mitosis, and that membrane trafficking is thought to be halted until cytokinesis (Foley and Kapoor, 2013; Yadav and Linstedt, 2011; Chen et al, 2012; Takatsu et al, 2013). Second, when Rab11 is disrupted, the molecular and functional integrity of spindle poles is compromised.…”

Section: Discussionmentioning

“…For time-lapse recording, the TfnR-EGFP expressing cells (Kondo et al. , 2012; Takatsu et al. , 2013) were treated with siRNAs as described, incubated in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid–buffered MEM, placed on a microscope stage prewarmed to 37°C, and then observed using an A1R-MP confocal microscope (Nikon, Melville, NY).…”

Section: Methodsmentioning