OpenCFU, a New Free and Open-Source Software to Count Cell Colonies and Other Circular Objects (original) (raw)

“…Debris might form clusters with pollen which make it difficult for software to delineate individual pollen to give a correct count (Mudd and Arathi, 2012). However, for many biological studies involving counting of small biological particle, a moderate deviation (lower than 20%) is often negligible compared to noise generated by other factors (Geissman, 2013). Yet, further studies are needed to: (1) improve method for separation of pollen from debris, (2) improve sensitivity of the pollen detection program, allowing for a better understanding of pollination process in oil palm, and (3) further application of this software to counting small circular objects in oil palm research (i.e.…”

Section: Figure 1 Various Images Resulting From Step-by-step Of Imagmentioning

“…One possibility is to use an image processing tool and analysis program which offer a more accurate, user friendly, and consistent estimation (Bechar et al, 1997;Aronne et al, 2001;Fonseca et al, 2002;Costa and Yang, 2009;Geissman et al, 2013;Mudd and Arathi, 2012). This tool is equipped with a particular computer software to scan pollen images, which previously captured as raw images by a camera.…”

Section: Introductionmentioning