Use of V(D)J recombination excision circles to identify T- and B-cell defects and to monitor the treatment in primary and acquired immunodeficiencies (original) (raw)

“…Thus, the results obtained from the TREC assay may be used to classify patients based on the status of their immunity, predicting susceptibility to infections. In this study, we found that the TREC levels were significantly lower in individuals with 22q11.2DS compared with healthy controls and tended to decrease with age due to the natural thymic involution, as has been previously shown (6,(22)(23)(24). Furthermore, lower TREC levels were found in 22q11.2DS patients with recurrent infections, regardless of their age, which probably contributes to the immunodeficiency in some patients.…”

Section: Discussionsupporting

“…Thus, the results obtained from the TREC assay may be used to classify patients based on the status of their immunity, predicting susceptibility to infections. In this study, we found that the TREC levels were significantly lower in individuals with 22q11.2DS compared with healthy controls and tended to decrease with age due to the natural thymic involution, as has been previously shown (6,(22)(23)(24). Furthermore, lower TREC levels were found in 22q11.2DS patients with recurrent infections, regardless of their age, which probably contributes to the immunodeficiency in some patients.…”

Section: Discussionsupporting

“…T-cell receptor excision circles (TRECs) and kappadeleting recombination excision circles (KRECs), such as circular DNA segments that persist in T cells and B cells, were recently considered markers of new lymphocyte output [32]. Kamae et al [33] indicated that low TREC and/or KREC levels are useful biomarkers that are associated with the overall survival rate in CVID patients.…”

Section: T-cell Abnormalities In Cvidmentioning

“…TRECs and KRECs are circular episomes of DNA that are formed into differentiating T- and B-cell progenitors during V(D)J rearrangement in the thymus and bone marrow. It is supposed that TREC and KREC measurement could be a valuable marker for lymphocyte neogenesis [6]. …”

Section: Introductionmentioning