Breaking and joining single-stranded DNA: the HUH endonuclease superfamily (original) (raw)

“…More recently, a group of related elements, ISCR (IS with a "common region") was described (reviewed in references 55 and 56). Although there has been no formal demonstration that these actually transpose, the CR is an orf that resembles the IS91 family HUH Tpases (41). The major feature of ISCR elements is that they are associated with a diverse variety of antibiotic resistance genes and, particularly in the case of Pseudomonas ISCR, with aromatic degradation pathways, both upstream and downstream of the Tpase orf.…”

Section: Is Related To Icementioning

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“…More recently, a group of related elements, ISCR (IS with a "common region") was described (reviewed in references 55 and 56). Although there has been no formal demonstration that these actually transpose, the CR is an orf that resembles the IS91 family HUH Tpases (41). The major feature of ISCR elements is that they are associated with a diverse variety of antibiotic resistance genes and, particularly in the case of Pseudomonas ISCR, with aromatic degradation pathways, both upstream and downstream of the Tpase orf.…”

Section: Is Related To Icementioning

“…ASMscience.org/MicrobiolSpectrum IS5 IS903 950-1,150 9 GG Y 1 DDE ISL2 850-1,200 2-3 1 ISH1 900-1,150 8 -GC 1 IS5 1,000-1,500 4 Ga/g 1 IS1031 850-1,050 3 GAa/g 1 IS427 800-1, (41). They are limited to two prokaryotic (IS91 and IS200/ IS605; He et al, this volume) and one eukaryotic (helitron; Thomas and Pritham this volume) TE family.…”

Section: Dedd Transposasesmentioning

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“…Thus, relaxases offer a potentially superior alternative to existing methods for protein conjugation to DNA nanostructures, due to their sequence specificity, orthogonality and also their natural capacity to form covalent bonds with standard oligonucleotides. Relaxases belong to the HUH protein superfamily,13 and the three‐dimensional structure of some relaxases is known 14. Their overall shape resembles a left hand, where the β‐sheet “palm” holds the HUH motif (histidine–hydrophobic amino acid–histidine) responsible for metal coordination, and the α‐helix “thumb” contains the catalytic tyrosine.…”

mentioning

“…The requirement of site-specific nicking of the AAVS1 trs site suggests that some of the steps resemble the terminal resolution reaction during AAV DNA replication. This process is a variation of the mechanism used by DNA relaxases during DNA conjugation (20,24,25). The first step in site-specific integration is the assembly of a Rep78/ Rep68-DNA complex guided by the recognition of GCTC repeats through the N-terminal origin-binding domain (OBD).…”

Section: Adeno-associated Virus (Aav)mentioning