Quantifying proteomes and their post-translational modifications by stable isotope label-based mass spectrometry (original) (raw)

“…Both the “top down” and “bottom up” proteomics are highly dependent on separation technologies [80, 81] to: (a) ensure extensive proteome coverage in a given time; (b) achieve higher analytical throughput; and (c) cover large dynamic concentration range, including even trace proteins. In some the most remarkable separations of complex peptide mixtures, multidimensional LC [81], small-particle technologies [82] and monolithic capillaries [84] were used. The high-power MS instrumentation, such as FTMS or Orbitrap, is additionally important in achieving the most comprehensive results.…”

Section: Problem-oriented Investigations: Integration Of Modern Anmentioning