DEAD-Box Helicases Form Nucleotide-Dependent, Long-Lived Complexes with RNA (original) (raw)

“…However, greater overexpression of Ded1 from an inducible GAL1 promoter represses translation, causing sequestration of eIF4E, eIF4G, and Pab1 in cytoplasmic granules (Hilliker et al 2011). Ded1 can form very stable complexes with RNA in vitro (Liu et al 2014) and it is important for resolving misfolded RNA structures and preventing higher-order structural contacts that would otherwise destabilize RNA architecture (Pan et al 2014).…”

Section: Mrna Recruitment Of the 43s Picmentioning

“…However, greater overexpression of Ded1 from an inducible GAL1 promoter represses translation, causing sequestration of eIF4E, eIF4G, and Pab1 in cytoplasmic granules (Hilliker et al 2011). Ded1 can form very stable complexes with RNA in vitro (Liu et al 2014) and it is important for resolving misfolded RNA structures and preventing higher-order structural contacts that would otherwise destabilize RNA architecture (Pan et al 2014).…”

Section: Mrna Recruitment Of the 43s Picmentioning

“…S7) (8). The weak ATPase activity at low Mg 2+ concentrations may result from slow release of RNA, limiting nucleotide turnover (46), or from Mg 2+ -dependent secondary structure stabilization (8). To isolate the effects of tertiary folding, we normalized the ATPase rates for each mutant by the corresponding rate for the quintuple mutant ribozyme at the same Mg 2+ concentration (Fig.…”

Section: +mentioning

“…2B) (20). Recycling of the enzyme requires hydrolysis of the bound ATP and release of inorganic phosphate (P i ) to allow efficient release of the tightly bound RNA strand (26–29). …”

Section: Dead-box Proteins: Remodeling One Duplex At a Timementioning