Quantitative Assessment of Cytosolic Salmonella in Epithelial Cells (original) (raw)

“…This activation has been observed in the context of infections with mutants that aberrantly enter the cytosol (Aachoui et al, 2013) after lysis of the SCV dependent on IFN-induced GTPases (Meunier et al, 2014). However, wild-type S. Typhimurium can also lyse their nascent vacuole following invasion of tissue culture epithelial cells and enter the cytosol where it can eventually hyper-replicate Knodler et al, 2014). Using a semi-quantitative single-cell analysis, Malik-Kale et al,showed that although cytosolic hyper-replication occurs in less than 20% of infected epithelial cells, it accounts for the majority of net intracellular replication (Malik-Kale et al, 2012).…”