Quantitative Analysis of rRNA Modifications Using Stable Isotope Labeling and Mass Spectrometry (original) (raw)

“…Incomplete reactions, side products, and unstable response factors prevent quantitative analysis by this approach. In consequence there is an increasing interest in derivatization-free MS/ MS-based approaches that allow quantitative analysis, 26,27 and which have recently included isotope labeling 28,29 So called hypermodified C derivatives are formed via further modification of C. At present, they comprise Cm, m 1 C, (found in all domains of life, predominantly in tRNA 1 ), m 3 C (in rRNA of Eubacteria 1 ), as well as 3-(3-amino-3-carboxypropyl)-C .axap3C/ 30,31 and m 1 acap 3 C 1 in rRNA of eukaryotes (Fig. 1).…”