Structure of a type IV secretion system (original) (raw)

“…Salmonella motors and C. jejuni ΔpflA, ΔpflB, ΔflgP, and ΔflgQ exhibited flexibility between the top and bottom parts of the motor. In these cases individual averages were constructed for the top and bottom segments and ultimately were merged into a composite structure in analogy to recent single-particle studies (62).…”

Section: Methodsmentioning

“…All other bands observed in the SDS–PAGE analysis of the complex purified from TrwB /VirD4 ‐producing cells are also present in the T4SS 3‐10 complex. This includes OmpC and OmpA, contaminants previously proven to be minor (Low et al , 2014). Thus, the complex purified from TrwB /VirD4 ‐producing cells is the T4SS 3‐10 complex to which VirD4 is bound.…”

Section: Resultsmentioning

“…The labelled sample of the complex was analysed using SDS–PAGE, and the fluorescence intensity of the bands was quantified. The TrwE /VirB10 protein as a part of the TrwH /VirB7 ‐TrwF /VirB9 ‐TrwE /VirB10 core complex alone or of the T4SS 3‐10 complex is known to be present in 14 copies (Fronzes et al , 2009b; Low et al , 2014). Therefore, the fluorescence signal quantified from the TrwE /VirB10 band was used as a reference to determine the stoichiometry of the other components.…”

Section: Resultsmentioning

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