Complexity and challenges in defining myeloid-derived suppressor cells (original) (raw)

“…The CD11b C cells are usefully split into two subsets using the Ly6G marker. 23,24 As previously reported, 12 there was a predominance of the CD11b…”

Section: Marked Alterations Of the Myeloid Compartmentsupporting

“…Numerous reports describe the presence of MDSC in murine tumors (reviewed in [22, 23]) and recent studies demonstrated that tumor associated MDSC have an important role in tumor progression [24, 25]. In humans, recent studies described the presence of MDSC in glioblastoma [26], urothelial carcinoma [27], pancreatic adenocarcinoma [7], and breast cancer [28].…”

Section: Regulation Of Mdsc Recruitment To Tumorsmentioning

“…Most studies on MDSC suppression have focused on cells in peripheral lymphoid organs (spleen, lymph nodes) and peripheral blood, mainly due to the technical challenges associated with MDSC isolation from tumors, which requires mechanical and enzymatic treatments that result in low recovery of MDSC and also impact cell function [23]. However, with technical advances in the isolation of myeloid cells from tumor tissues by using more gentle disaggregation of tissues with gentleMACS disaggregator ® (Miltenyi) or optimized dissociation protocols (one example described in [43]), it became clear that the mechanisms of MDSC action in the tumor site are different from the ones employed by these cells in peripheral lymphoid organs.…”

Section: Mechanisms Of Mdsc-mediated Immune Suppression Are Localizatmentioning

“…3 MDSCs are a heterogeneous population of immature myeloid cells that accumulate in blood, lymphoid organs, and tumor tissue under several pathologic conditions, including cancer. 3,4 MDSCs are generally characterized by being positive for CD33 and CD11b and low or negative for HLA-DR. 5 This population can be divided in 2 subgroups with different morphology. Monocytic (MO)-MDSCs are mononuclear and CD14 positive, whereas granulocytic or polymorphonuclear (PMN)-MDSCs are CD14 negative.…”

Section: Introductionmentioning

“…Monocytic (MO)-MDSCs are mononuclear and CD14 positive, whereas granulocytic or polymorphonuclear (PMN)-MDSCs are CD14 negative. 3,5 Both populations contribute to tumor immune escape by inducing immune suppression and tolerance through a variety of mechanisms, such as production of nitric oxide and reactive oxygen species, depletion of arginine and cysteine, and induction of regulatory T cells (Tregs). [6][7][8][9] However, their regulation and dynamics are poorly understood, especially in humans.…”

Section: Introductionmentioning