Novel 89Zr cell labeling approach for PET-based cell trafficking studies (original) (raw)

“…Longer lived PET isotopes such as 89 Zr have recently been used in combination with more specific cell labeling strategies such as so-called "click chemistry" to enhance signal half-life and specificity with promising results. [46][47][48] However, prolonged exposure to ionizing radiation remains problematic for cell-trafficking approaches in the clinic. Perfluorcarbon…”

Section: Discussionmentioning

“…The percentage of viable DCs was assessed with the trypan blue exclusion test by means of directly counting the cells, using a microscope as previously reported [15,16]. Experiments were performed 5 times.…”

Section: Cell Viability Assaysmentioning

“…However, recently, a novel cell labeling agent (ie, Zirconium-89) has emerged as an attractive PET radionuclide for cell labeling application due to its high spatial resolution and 78.4-hour half-life that may allow monitoring of administered cells up to a 2-to 3-week period. 17 Importantly, both MRI and PET provide low-resolution imaging at the cellular or subcellular level. Fluorescence imaging with nanoparticles is another noninvasive imaging method for in vivo tracking.…”

Section: Introductionmentioning