Coordinated Dynamics of RNA Splicing Speckles in the Nucleus (original) (raw)

“…In order to test this hypothesis, we used the SRRM2 tr0 and SRRM2 tr10 cells as a model, which allowed us to simultaneously detect SON, SRRM2 and an additional NS marker in the same cell. We chose SRRM1, which is used as a marker for NS in immunofluorescence experiments (8,9,14,25,68) and located at ICGs in electron microscopy experiments (69); RBM25, which is one of two recommended factors to mark NS by the Human Protein Atlas (70) (the other being SRRM2),localizes to NS through its RE/RD-rich mixedcharge domain (71) that was recently shown to target proteins to NS (72) and PNN, which localizes to nuclear speckles in human cells (63)(64)(65)73).…”

Section: Fig 3 Srrm2 Truncation#10 Leads To Loss Of Sc35 Domains Bumentioning

“…Nuclear speckles (NS) are membraneless nuclear bodies in the interchromatin-space of the nucleus that contain high concentrations of RNA-processing and some transcription factors but are devoid of DNA (3). Under normal conditions, they appear as irregularly shaped, dynamic structures that show hallmarks of phase-separated condensates, such as fusion and deformation under pressure in living cells (5,9). Despite their prevalence, the function of NS remains largely unknown, although they have been proposed to act as reservoirs for splicing factors, and association with NS have been shown to correlate with enhanced transcription and RNA processing (4,5).…”

Section: Introductionmentioning