5′ UTR m6A Promotes Cap-Independent Translation (original) (raw)

“…78 It has also become clear that depending on the context of the modification and its localization, for instance in the 5 0 UTR or 3 0 UTR of a transcript, the same protein can coordinate with different cellular machinery and result in very different outcomes. 10,11 While substantial progress has been made in mapping RNA modification sites with high throughput sequencing-based approaches, some major challenges remain. First, in the majority of cases, even if single base resolution can be achieved, we cannot determine the absolute stoichiometry of modification at a given site.…”