The position of lysosomes within the cell determines their luminal pH (original) (raw)

“…3G). The latter likely represent lysosomes that are less acidic, such as those described in the periphery of nonneuronal cells (40). We also observed more diffuse SEP fluorescence in the distal axon, including the growth cone ( Fig.…”

Section: Resultssupporting

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“…3G). The latter likely represent lysosomes that are less acidic, such as those described in the periphery of nonneuronal cells (40). We also observed more diffuse SEP fluorescence in the distal axon, including the growth cone ( Fig.…”

Section: Resultssupporting

“…3). This heterogeneity resembles that recently reported for lysosomes in nonneuronal cells (40). Lysosomes in the proximal and distal parts of the axon depend on the BORC-Arl8-SKIP-kinesin-1 complex for anterograde transport.…”

Section: Discussionmentioning

“…This suggests that the requirements for cell-to-cell fusion and virus-to-cell fusion are different. The luminal pH of most lysosomes ranges from 4.5 to 5 (45). In contrast, we utilized phosphate-buffered saline (PBS) at pH 4 in the cell-to-cell …”

Section: Discussionmentioning

“…A recent study has shown that juxtanuclear lysosomes are more acidic and have higher cathepsin L activity than peripheral lysosomes (Johnson et al, 2016). This heterogeneity could be due to the stabilization of the V-ATPase subunit V1G1 by RILP (Johnson et al, 2016).…”

Section: Autophagymentioning