Protein synthetic machinery and mRNA in regenerating tips of spinal cord axons in lamprey (original) (raw)

“…Together with previous observation connecting deficiencies in NCT to axonal guidance defects in flies 49 , our data are particularly interesting as they functionally link the cytoskeleton with NCT and mRNA post-transcriptional regulation. Axon growth is a complex process that requires active cytoskeletal growth 50 , fueled by the increased expression, transport, and local translation of several cytoskeletal-related mRNAs 51,52 . Defects in the localization and local translation of mRNAs at the tip of axons and dendrites has been described in other neurological diseases, such as SMA and Fragile X syndrome 53,54 , supporting the relevance of these defects in disease pathogenesis.…”

Section: Discussionmentioning

“…Together with previous observation connecting deficiencies in NCT to axonal guidance defects in flies 49 , our data are particularly interesting as they functionally link the cytoskeleton with NCT and mRNA post-transcriptional regulation. Axon growth is a complex process that requires active cytoskeletal growth 50 , fueled by the increased expression, transport, and local translation of several cytoskeletal-related mRNAs 51,52 . Defects in the localization and local translation of mRNAs at the tip of axons and dendrites has been described in other neurological diseases, such as SMA and Fragile X syndrome 53,54 , supporting the relevance of these defects in disease pathogenesis.…”

Section: Discussionmentioning

“…One of the most surprising findings from this study is the robust and complex transcriptional responses occurring in the lamprey brain after SCI. Previous studies in lamprey revealed SCI-induced changes in expression levels of genes related to axon growth and guidance within the RS neuron somata located in the brain and in the tips of regenerating axons 33 , 51 , 52 , 76 , 77 , 80 . Here, we confirmed and extended these observations to demonstrate global supraspinal changes in gene expression throughout the course of functional recovery.…”

Section: Discussionmentioning

“…Together with previous observation connecting deficiencies in NCT to axonal guidance defects in flies 36 , our data are particularly interesting as they functionally link the cytoskeleton with NCT and mRNA post-transcriptional regulation. Axon growth is a complex process that requires active cytoskeletal growth 37 , fueled by the increased expression, transport, and local translation of several cytoskeletal-related mRNAs 38,39 . Defects in the localization and local translation of mRNAs at the tip of axons and dendrites has been described in other neurological diseases, such as SMA and Fragile X syndrome 40,41 , supporting the relevance of these defects in disease pathogenesis.…”

Section: Discussionmentioning