Multicolour Multilevel STED nanoscopy of Actin/Spectrin Organization at Synapses (original) (raw)

“…It is worth noting that, because a dendrite often contained both periodic and irregular regions, the fraction of dendrites exhibiting at least one periodic region was quite large (>80%), consistent with previous results (15,16). We also observed heterogeneity among dendrites with some dendrites being covered by the 1D MPS structure substantially more extensively than the others.…”

Section: Resultssupporting

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“…It is worth noting that, because a dendrite often contained both periodic and irregular regions, the fraction of dendrites exhibiting at least one periodic region was quite large (>80%), consistent with previous results (15,16). We also observed heterogeneity among dendrites with some dendrites being covered by the 1D MPS structure substantially more extensively than the others.…”

Section: Resultssupporting

“…We and others have also observed the 1D MPS in dendrites (9,10,13,15,16). Here, we systematically investigated the distributions of four MPS components, βII-spectrin, βIII-spectrin, actin and adducin, in cultured hippocampal neurons.…”

Section: Discussionmentioning

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“…27 Despite its short time from the invention, STED microscopy has made many contributions to the observation of subcellular structures such as endoplasmic reticulum, 28 mitochondria 29,30 and cytoskeletons. 31,32 Because of complicated components involved in signal transduction between cells, dualcolor STED [33][34][35] and even multi-color STED 36,37 have been established to study several active complexes at the same time. Besides, 3D STED is also available for biologists which provides 3D imaging with the resolution of 45 Â 45 Â 108 nm in a cell originally.…”

Section: Stimulated Emission Depletion (Sted) Microscopymentioning