Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review (original) (raw)

“…Furthermore, chemicals or small molecules including cytokines are major factors in the induction of cell differentiation [25][26][27][28]. Francisco et al found that human preadipocytes and ASCs were excellent models for studying adipogenesis and obesity-related metabolic alterations and were appropriate for revealing adipocyte renewal, development and depot-specific differences [4]. Therefore, we focused on uncovering the molecular regulation mechanism as to how miRNAs regulate adipogenic differentiation of hASCs.…”

Section: Discussionmentioning

“…Monitoring the alteration of adipose-derived stem cells (ASCs) can provide a promising future in the field of tissue engineering and regenerative medicine [3]. First, ASCs are recruited by certain stimulation, following which they are transformed into preadipocytes and then ultimately turn into mature adipocytes [4]. During adipogenesis, several transcription factors such as peroxisome proliferator-activated receptor-γ (PPARγ) and members of the CCAAT/enhancer-binding family of proteins (c/EBPα) participate in regulating adipogenesis [5,6].…”

Section: Introductionmentioning

“…The 3T3-L1 is a cell line derived from mouse 3T3 cells, which is an adipocyte with fibroblast like morphology (Ruiz-Ojeda, Ruperez, Gomez-Llorente, Gil, & Aguilera, 2016). The fibroblast cell lines viz.…”

Section: In Vitro Wound Healing Assaymentioning

“…Results from these studies are not as useful for applications on human health as expected, given the physiological and metabolic differences among species [2]. Human primary cultured cells are the best resource to understand human adipocyte biology in deranged metabolic states [2], but cannot inform about cell events that occur during prenatal life.…”

Section: Introductionmentioning