Inducible HIV RNA transcription assays to measure HIV persistence: pros and cons of a compromise (original) (raw)

“…2 B). Only a median of 25% [ [15] , [16] , [17] , [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] ] of all positive wells were positive for all three RNA transcripts ( Fig. 2 B), suggesting that the majority of cells producing ca-RNA are unable to produce viral particles.…”

Section: Resultsmentioning

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“…2 B). Only a median of 25% [ [15] , [16] , [17] , [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] ] of all positive wells were positive for all three RNA transcripts ( Fig. 2 B), suggesting that the majority of cells producing ca-RNA are unable to produce viral particles.…”

Section: Resultsmentioning

“…2 B), suggesting that the majority of cells producing ca-RNA are unable to produce viral particles. Some wells were positive for one of the three transcripts only (27% [ [21] , [22] , [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] ] gag ca-RNA only, 0% [0–3] tat-rev ca-RNA only, and 4% [ [2] , [3] , [4] , [5] , [6] ] cf-RNA only), which could be explained by different activation status of the infected cells at the time of nucleic acid extraction, distinct expression kinetics of the different transcripts, or deletions or other proviral sequence variation.…”

Section: Resultsmentioning

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