Hypoxic tumour cell-derived exosomal miR-340-5p promotes radioresistance of oesophageal squamous cell carcinoma via KLF10 (original) (raw)

“…γH2A is an established marker of DNA damage ( 23 ). Relative to mock-silenced cells, DCTPP1-deficient induced BRCA cells had significantly higher γH2A levels ( Figure 5B ).…”

Section: Resultsmentioning

“…Then, cultured for 2 weeks later, 1% crystal violet was applied in staining clonogenic cells. The clonogenic survival curves were generated according to the following formula model: SF = 1 − (1 − e −kd ) n as previously described [ 17 ].…”

Section: Methodsmentioning

“…The immunofluorescence of γ H2AX was detected by the anti- γ H2AX primary antibody (1 : 500, Abcam, UK) and the detailed process was described in the previous protocol [ 17 ]. Cells were snapped under a confocal fluorescence microscope (Leica).…”

Section: Methodsmentioning

“… 40 Recently, it has been shown that hypoxic tumor cell-derived exosomal miR-340-5p conferred radioresistance in ESCC by targeting kruppel-like factor 10 (KLF10)/UV radiation resistance-associated gene protein (UVRAG). 57 The level of miR-340-5p in the plasma exosomes was closely related to radiotherapy response and prognosis. miR-340-5p might be a therapeutic target for overcoming radioresistance in ESCC.…”

Section: Exosomes Promote Ec Therapy-resistancementioning

“… 70 In addition, circulatory exosomal miR-340-5p and miR-339-5p might be promising biomarkers for prediction of radiotherapy response and prognosis in patients with ESCC. 57 , 58 Exosomal miRNAs will have great potential application in tumor individualized treatment.…”

Section: Exosomal Mirnasmentioning