Ahmed Youssef | Suez Canal Uni. (original) (raw)
Papers by Ahmed Youssef
Journal of Applied Microbiology, 2021
Aim This study aimed to identify genotype enterotoxigenic antimicrobial-resistant Staphylococcus ... more Aim This study aimed to identify genotype enterotoxigenic antimicrobial-resistant Staphylococcus aureus species, mainly methicillin-resistant S. aureus (MRSA) and vancomycin-resistant S. aureus (VRSA) among commensal rodents. Methods and results A total of 280 samples were collected from nasal and mouth swabs, heart blood, intestinal content and lung tissues of 56 commensal rodents trapped from North Sinai, Egypt. Antimicrobial susceptibility testing was performed to bacteriologically identified S. aureus isolates against 15 antimicrobial agents by disc diffusion method. Detection was conducted for identifying coagulase gene (coA), antimicrobial-resistant genes (mecA and vanA/B), enterotoxigenic and virulence determinant genes (hlg, seb, sed and see) among the MRSA and VRSA isolates. Results Staphylococcus aureus species were isolated from 24 (42.86%) out of 56 rodents. Phenotypic examination revealed that all the isolates were multidrug-resistant, whereas two isolates were multiple...
Veterinary World, 2016
The main objectives of this study were to determine the incidence of Sarcocystis sp. infection in... more The main objectives of this study were to determine the incidence of Sarcocystis sp. infection in cattle and buffalo carcasses slaughtered at El-Kharga abattoir, New Valley Governorate, Egypt. Materials and Methods: The slaughtered animals were daily inspected for Sarcocystis macrocysts through a year (2015). Macroscopic Sarcocystis was detected from a total of 2120 cattle and buffalo carcasses. In addition, 100 meat samples were collected from female cattle and buffalo (50 each) and were examined microscopically for sarcocystosis. Results: The overall incidence of Sarcocystis macrocyst among bovine carcasses was 159/2120 (7.5%). Total incidence in cattle was 149/2000 (7.45%), whereas it was 10/120 (8.33%) in buffalo carcasses. Concerning gender, the overall prevalence of Sarcocystis infection was 127/1790 (7.09%) in male and 32/330 (9.69%) in females bovine carcasses. The highest detection rate of Sarcocystis lesions was from the esophagus (76.3%) followed by throat muscles (35.3%), tongue (33.8%), and diaphragm muscles (18.71%). Macrocysts from cattle were identified to Sarcocystis hirsuta, whereas Sarcocystis fusiformis was identified from buffalo carcasses. By microscopic examination, 18 (36%) of 50 female cattle carcasses harbor Sarcocystis sp., whereas 11 (22%) of buffalo carcasses were harbored Sarcocystis microcysts. Conclusion: A high incidence of Sarcocystis infection was detected among slaughtered bovines in El-Kharga abattoir, Egypt. Sarcocystis macrocysts were a higher incidence in female elder animals macrocysts were identified to S. hirsuta in cattle and S. fusiformis in buffaloes. Sarcocystosis constitute a major cause of economic losses at El-Kharga abattoir. Beef meat may carry health risks to consumers.
This study aimed to investigate Salmonella enterica serovar Typhimurium (ST) and Salmonella enter... more This study aimed to investigate Salmonella enterica serovar Typhimurium (ST) and Salmonella enterica serovar Enteritidis (SE) infections of diarrheic calves in bovine dairy herds and their farm workers. Antimicrobial drug resistance profile of the isolates was determined by standard disc diffusion assay. Fecal swabs were collected from diarrheic calves aged from one week to three months (Median age 33.5 days); from three farms representing cattle, native breed buffalo and crossbreed buffalo farms. Each farm was visited every three months for one year, from March 2011 to February 2012. Collected Swabs were examined by conventional bacterial culture and serotyped by multiplex PCR. Results showed that Salmonella was isolated from 42/255 (18.66%) diarrheic calves and 27/35 (77.14%) calves died from diarrhea. Incidence of ST was significantly higher in calves died from diarrhea than diarrheic calves (P>0.001). On farm level, isolation rate of Salmonella was significantly higher in dead calves in crossbreed buffalo farm than native breed buffalo and cattle farms (P>0.001). On other hand, the incidence of Salmonella among stool swabs of farm workers caring the calves was 8.13%; with isolation rate of (2.32%) ST, (1.16%) SE and (4.65%) other Salmonella serovas. Isolates showed a high percentage of multi-drug resistant profile (63.88%). All isolates were completely resistant to oxytetracycline, danofloxacin and oxacillin. Enrofloxacin was the drug of choice. In conclusion, a high incidence of mutli-drug resistant Salmonella infections was detected in cattle and buffalo calves that might be associated with high mortality rate in calves and zoonotic potential to the farm workers. Adequate hygienic measures, proper immunization programs and regular training to the labor should be applied.
International Food Research Journal
This article aimed to investigate bovine tuberculosis in cattle and buffalo slaughtered in Ismail... more This article aimed to investigate bovine tuberculosis in cattle and buffalo slaughtered in Ismailia city abattoir, Egypt and examining abattoir workers for tuberculosis infection. Carcasses were examined for tuberculosis-like lesions for one year starting from March 21 st 2009. Tuberculosis-like lesions were examined by Ziehl-Neelsen staining and histopathological techniques. Results revealed that of 13,866 slaughtered bovine, tuberculosis-like lesions were detected in 86 (0.6%) of carcasses including 2 (2.3%) generalized and 84 (97.7%) localized lesions. Microscopic examination for Acid-Fast Bacilli (AFB) and histopathological examination showed that 38 (0.3%) carcasses and 50 (43.9%) of tuberculosis-like lesions were confirmed to be tuberculosis. Lesions in the lung and its associated lymph nodes were the most predominant (64.8%). The tuberculosis-like lesions were grossly detected to be higher among cattle (0.7%) than buffalo (0.5%). There were significant differences between mal...
An epidemiological investigation was carried out to evaluate the current situation of Rift Valley... more An epidemiological investigation was carried out to evaluate the current situation of Rift Valley Fever disease (RVF) in Egypt. The results of study showed that vaccinated sheep from El-Qalyubia had lower percent of antibodies against RVF with non-protective titer (1/20) while, Vaccinated sheep, goat, cattle and buffaloes from Marsa Matruh and El-Monufia revealed higher percent of antibodies against RVF with protective titer 1/40 using agar gel precipitation test (AGPT), serum neutralization test (SNT) and enzyme linked immunosorbent assay (ELISA). On the other hand non-vaccinated sheep, goats, cattle, buffaloes and camels from El-Qalyubia, El-Dakahlia, El-Sharqia and Kafr El-Sheikh exihibted different rates of antibodies against RVF among governorates in which higher rate occur in El-Sharqia followed by El-Dakahlia then Kafr El-Sheikh and lastly Qualubya. The virological analysis showed no virus isolate from sera samples of all governorates or from liver suspension of rats collecte...
Natural Product Research, 2013
Methanolic extract of Capparis sinaica Veill was tested for its in vitro antiviral activity again... more Methanolic extract of Capparis sinaica Veill was tested for its in vitro antiviral activity against highly pathogenic avian influenza strain H5N1 using plaque inhibition assay in Madin-Darby canine kidney. The results indicated that the extract possessed potent antiviral activity (100% inhibition at the concentration of 1 μg/ml). Based on this result, C. sinaica Veill was selected for further study by applying bioactivity-guided fractionation to isolate its antiviral principles. The fractions eluted with EtOAc and 25% MeOH in EtOAc were found to hold the antiviral activity. Further chromatographic separation of the fractions holding the antiviral activity led to the isolation of quercetin (1), isoquercetin (2) and rutin (3) for the first time from this species. The isolates showed reduction in the virus titre by 68.13%, 79.66% and 73.22% inhibition at a concentration of 1 ng/ml, respectively.
Natural Product Research, 2013
Some Egyptian plants were screened against highly pathogenic avian influenza strain H5N1 using pl... more Some Egyptian plants were screened against highly pathogenic avian influenza strain H5N1 using plaque inhibition assay in Madin-Darby canine kidney. The results indicated that the extracts of Red Sea grass Thallasodendron ciliatum possessed potent antiviral activity (100% inhibition at the concentration of 1 μg mL⁻¹). The bioactivity-guided fractionations led to the isolation of a new diglyceride ester (1) along with asebotin (2) for the first time from the plant. The two isolates showed reduction of virus titre by 67.26% and 53.81% inhibition at concentration of 1 ng mL⁻¹, respectively.
American Journal of Animal and Veterinary Sciences, 2014
This study aimed to illustrate the potential role of wild migratory quails in the epidemiology of... more This study aimed to illustrate the potential role of wild migratory quails in the epidemiology of some zoonotic pathogens in Egypt. Ninety four apparently healthy migratory quails were collected from public markets after being trapped during hunting season from the North Sinai. Oral, cloaca and organs (liver, lung and heart) swabs were collected for bacteriological and viral examination. Bacteriological examination revealed high percentage of lactose fermenter bacteria (48.9%) including E. coli spp. (37.2%) and Salmonella spp. (24.4%). Three out of 12 (25%) of Salmonella spp. Isolates were S. enterica subspecies typhimurium. Other bacteria isolation rate was differed such as Staphylococcusaureus spp. (19.1) % , Proteus spp. (7.4%), Listeria spp. (4.2%) and Klepsila spp. (3.1%). E. coli spp. and Salmonella spp. isolates were further examined by API20and PCR techniques. Antibiogram testing against E. coli spp. and 12 Salmonella spp. isolates, revealed multi-drug resistance pattern and high sensitivity to enrofloxacin, nitrofuranation and ofloxacine. Parasitological examination revealed that 35 (37.2%) quails were infected with chewing lice (Phithiraptera spp.). Intestinal infection with Heterakis spp. and/or Ascarida spp. Was detected in 27 (28.7%) quails. All quails were negative for avian influenza virus by direct detection by Rt-PCR for oral and cloaca swabs followed by inoculation in embryonated chicken eggs. In conclusion, the migratory quails might play a potential role in dispersion of zoonosis in Egypt.
Journal of Veterinary Advances, 2015
RVF virus is a member of the genus Phlebovirus, belongs to the Bunyaviridea family, RVFV is an en... more RVF virus is a member of the genus Phlebovirus, belongs to the Bunyaviridea family, RVFV is an enveloped virus with a diameter of 90 to 110 nm and a core element of 80 to 85 nm. The viral genome consists of single-stranded, segmented tripartited RNA, among which the large (L) and medium (M) segments are negative polarity, and the small (S) segment is ambisense polarity. The RNA of S segment of ZH501VSVRI was extracted then it converts to cDNA and ampilified by Conventional PCR. Electrophoresis of PCR product was done. The DNA band was sliced from the gel and purified followed by sequencing. The resultant sequence was aligned with those of previously characterized RVF viruses by using Bioedit program which revealed three nucleotide substitutions and these lead to presence of three mutations in translating amino acid in non-structure S protein (Ns S).
Veterinary World, 2021
Background and Aim: Poultry infections with H9N2 avian influenza viruses (AIVs) are endemic in Eg... more Background and Aim: Poultry infections with H9N2 avian influenza viruses (AIVs) are endemic in Egypt. This study determined the genetic changes in the sequences of H9N2 AIVs isolated from chicken and quails in Egypt, including determining genetic reassortment and detecting the main genetic changes in hemagglutinin (HA) and neuraminidase (NA) genes. Materials and Methods: Swab samples were collected from chicken and quails, examined through reverse transcription-polymerase chain reaction, and AIVs from positive samples were isolated in embryonated chicken eggs. Complete genome sequencing and phylogenetic analyses were conducted for two H9N2 AIV isolates, and sequences of HA and NA gene segments were analyzed in another two isolates. Results: A novel reassortant virus was identified from a commercial chicken flock (A/chicken/Egypt/374V/2016) and quails from a live bird market (A/quail/Egypt/1253V/2016). The reassortant viruses acquired four genome segments from the classic Egyptian H9...
Tropical Medicine and Health, 2014
Ochratoxin A (OTA) is a serious problem affecting the poultry industry as well as a health hazard... more Ochratoxin A (OTA) is a serious problem affecting the poultry industry as well as a health hazard for humans consuming contaminated poultry meat. The aim of this study was to determine OTA levels in the feed used in some poultry farms in Ismailia, Egypt and in organs of birds from those farms. Commercial ELISA kits were used to detect levels of OTA in poultry feed samples and main feed ingredients as well as its residues in bird's tissues (Kidney, liver and muscles). Poultry feed samples were also tested for mycological contaminations. The most common commercial toxin binders were also evaluated in vitro. OTA concentration in poultry feed and ingredients ranged from 7.10-20.72 µg/kg. Soybean meal samples showed the lowest concentration, while rations formulated within the farm had the highest mean concentration. Producers using different toxin binders had a significant lower OTA concentration in their rations (p<0.05) as compared to those not using binders. OTA residues in different tissues were high in kidney and liver as compared to muscles. Asprigillus flavus was isolated from 80% of the examined feed samples. Different binders showed different abilities to bind OTA in vitro with highest binding capacity for yeast as compared to other commercially available binders.
Intervirology, 2009
... Adel El-Hamid Ahmed Serwah d Yoshitake Hayashi a ... Am J Trop Med Hyg 2000; 63: 236241. 11 ... more ... Adel El-Hamid Ahmed Serwah d Yoshitake Hayashi a ... Am J Trop Med Hyg 2000; 63: 236241. 11 Darwish MA, Faris R, Clemens JD, Rao MR, Edelman R: High seroprevalence of hepatitis A, B, C, and E viruses in residents in an Egyptian village in the Nile Delta: a pilot study. ...
Journal of Applied Microbiology, 2021
Aim This study aimed to identify genotype enterotoxigenic antimicrobial-resistant Staphylococcus ... more Aim This study aimed to identify genotype enterotoxigenic antimicrobial-resistant Staphylococcus aureus species, mainly methicillin-resistant S. aureus (MRSA) and vancomycin-resistant S. aureus (VRSA) among commensal rodents. Methods and results A total of 280 samples were collected from nasal and mouth swabs, heart blood, intestinal content and lung tissues of 56 commensal rodents trapped from North Sinai, Egypt. Antimicrobial susceptibility testing was performed to bacteriologically identified S. aureus isolates against 15 antimicrobial agents by disc diffusion method. Detection was conducted for identifying coagulase gene (coA), antimicrobial-resistant genes (mecA and vanA/B), enterotoxigenic and virulence determinant genes (hlg, seb, sed and see) among the MRSA and VRSA isolates. Results Staphylococcus aureus species were isolated from 24 (42.86%) out of 56 rodents. Phenotypic examination revealed that all the isolates were multidrug-resistant, whereas two isolates were multiple...
Veterinary World, 2016
The main objectives of this study were to determine the incidence of Sarcocystis sp. infection in... more The main objectives of this study were to determine the incidence of Sarcocystis sp. infection in cattle and buffalo carcasses slaughtered at El-Kharga abattoir, New Valley Governorate, Egypt. Materials and Methods: The slaughtered animals were daily inspected for Sarcocystis macrocysts through a year (2015). Macroscopic Sarcocystis was detected from a total of 2120 cattle and buffalo carcasses. In addition, 100 meat samples were collected from female cattle and buffalo (50 each) and were examined microscopically for sarcocystosis. Results: The overall incidence of Sarcocystis macrocyst among bovine carcasses was 159/2120 (7.5%). Total incidence in cattle was 149/2000 (7.45%), whereas it was 10/120 (8.33%) in buffalo carcasses. Concerning gender, the overall prevalence of Sarcocystis infection was 127/1790 (7.09%) in male and 32/330 (9.69%) in females bovine carcasses. The highest detection rate of Sarcocystis lesions was from the esophagus (76.3%) followed by throat muscles (35.3%), tongue (33.8%), and diaphragm muscles (18.71%). Macrocysts from cattle were identified to Sarcocystis hirsuta, whereas Sarcocystis fusiformis was identified from buffalo carcasses. By microscopic examination, 18 (36%) of 50 female cattle carcasses harbor Sarcocystis sp., whereas 11 (22%) of buffalo carcasses were harbored Sarcocystis microcysts. Conclusion: A high incidence of Sarcocystis infection was detected among slaughtered bovines in El-Kharga abattoir, Egypt. Sarcocystis macrocysts were a higher incidence in female elder animals macrocysts were identified to S. hirsuta in cattle and S. fusiformis in buffaloes. Sarcocystosis constitute a major cause of economic losses at El-Kharga abattoir. Beef meat may carry health risks to consumers.
This study aimed to investigate Salmonella enterica serovar Typhimurium (ST) and Salmonella enter... more This study aimed to investigate Salmonella enterica serovar Typhimurium (ST) and Salmonella enterica serovar Enteritidis (SE) infections of diarrheic calves in bovine dairy herds and their farm workers. Antimicrobial drug resistance profile of the isolates was determined by standard disc diffusion assay. Fecal swabs were collected from diarrheic calves aged from one week to three months (Median age 33.5 days); from three farms representing cattle, native breed buffalo and crossbreed buffalo farms. Each farm was visited every three months for one year, from March 2011 to February 2012. Collected Swabs were examined by conventional bacterial culture and serotyped by multiplex PCR. Results showed that Salmonella was isolated from 42/255 (18.66%) diarrheic calves and 27/35 (77.14%) calves died from diarrhea. Incidence of ST was significantly higher in calves died from diarrhea than diarrheic calves (P>0.001). On farm level, isolation rate of Salmonella was significantly higher in dead calves in crossbreed buffalo farm than native breed buffalo and cattle farms (P>0.001). On other hand, the incidence of Salmonella among stool swabs of farm workers caring the calves was 8.13%; with isolation rate of (2.32%) ST, (1.16%) SE and (4.65%) other Salmonella serovas. Isolates showed a high percentage of multi-drug resistant profile (63.88%). All isolates were completely resistant to oxytetracycline, danofloxacin and oxacillin. Enrofloxacin was the drug of choice. In conclusion, a high incidence of mutli-drug resistant Salmonella infections was detected in cattle and buffalo calves that might be associated with high mortality rate in calves and zoonotic potential to the farm workers. Adequate hygienic measures, proper immunization programs and regular training to the labor should be applied.
International Food Research Journal
This article aimed to investigate bovine tuberculosis in cattle and buffalo slaughtered in Ismail... more This article aimed to investigate bovine tuberculosis in cattle and buffalo slaughtered in Ismailia city abattoir, Egypt and examining abattoir workers for tuberculosis infection. Carcasses were examined for tuberculosis-like lesions for one year starting from March 21 st 2009. Tuberculosis-like lesions were examined by Ziehl-Neelsen staining and histopathological techniques. Results revealed that of 13,866 slaughtered bovine, tuberculosis-like lesions were detected in 86 (0.6%) of carcasses including 2 (2.3%) generalized and 84 (97.7%) localized lesions. Microscopic examination for Acid-Fast Bacilli (AFB) and histopathological examination showed that 38 (0.3%) carcasses and 50 (43.9%) of tuberculosis-like lesions were confirmed to be tuberculosis. Lesions in the lung and its associated lymph nodes were the most predominant (64.8%). The tuberculosis-like lesions were grossly detected to be higher among cattle (0.7%) than buffalo (0.5%). There were significant differences between mal...
An epidemiological investigation was carried out to evaluate the current situation of Rift Valley... more An epidemiological investigation was carried out to evaluate the current situation of Rift Valley Fever disease (RVF) in Egypt. The results of study showed that vaccinated sheep from El-Qalyubia had lower percent of antibodies against RVF with non-protective titer (1/20) while, Vaccinated sheep, goat, cattle and buffaloes from Marsa Matruh and El-Monufia revealed higher percent of antibodies against RVF with protective titer 1/40 using agar gel precipitation test (AGPT), serum neutralization test (SNT) and enzyme linked immunosorbent assay (ELISA). On the other hand non-vaccinated sheep, goats, cattle, buffaloes and camels from El-Qalyubia, El-Dakahlia, El-Sharqia and Kafr El-Sheikh exihibted different rates of antibodies against RVF among governorates in which higher rate occur in El-Sharqia followed by El-Dakahlia then Kafr El-Sheikh and lastly Qualubya. The virological analysis showed no virus isolate from sera samples of all governorates or from liver suspension of rats collecte...
Natural Product Research, 2013
Methanolic extract of Capparis sinaica Veill was tested for its in vitro antiviral activity again... more Methanolic extract of Capparis sinaica Veill was tested for its in vitro antiviral activity against highly pathogenic avian influenza strain H5N1 using plaque inhibition assay in Madin-Darby canine kidney. The results indicated that the extract possessed potent antiviral activity (100% inhibition at the concentration of 1 μg/ml). Based on this result, C. sinaica Veill was selected for further study by applying bioactivity-guided fractionation to isolate its antiviral principles. The fractions eluted with EtOAc and 25% MeOH in EtOAc were found to hold the antiviral activity. Further chromatographic separation of the fractions holding the antiviral activity led to the isolation of quercetin (1), isoquercetin (2) and rutin (3) for the first time from this species. The isolates showed reduction in the virus titre by 68.13%, 79.66% and 73.22% inhibition at a concentration of 1 ng/ml, respectively.
Natural Product Research, 2013
Some Egyptian plants were screened against highly pathogenic avian influenza strain H5N1 using pl... more Some Egyptian plants were screened against highly pathogenic avian influenza strain H5N1 using plaque inhibition assay in Madin-Darby canine kidney. The results indicated that the extracts of Red Sea grass Thallasodendron ciliatum possessed potent antiviral activity (100% inhibition at the concentration of 1 μg mL⁻¹). The bioactivity-guided fractionations led to the isolation of a new diglyceride ester (1) along with asebotin (2) for the first time from the plant. The two isolates showed reduction of virus titre by 67.26% and 53.81% inhibition at concentration of 1 ng mL⁻¹, respectively.
American Journal of Animal and Veterinary Sciences, 2014
This study aimed to illustrate the potential role of wild migratory quails in the epidemiology of... more This study aimed to illustrate the potential role of wild migratory quails in the epidemiology of some zoonotic pathogens in Egypt. Ninety four apparently healthy migratory quails were collected from public markets after being trapped during hunting season from the North Sinai. Oral, cloaca and organs (liver, lung and heart) swabs were collected for bacteriological and viral examination. Bacteriological examination revealed high percentage of lactose fermenter bacteria (48.9%) including E. coli spp. (37.2%) and Salmonella spp. (24.4%). Three out of 12 (25%) of Salmonella spp. Isolates were S. enterica subspecies typhimurium. Other bacteria isolation rate was differed such as Staphylococcusaureus spp. (19.1) % , Proteus spp. (7.4%), Listeria spp. (4.2%) and Klepsila spp. (3.1%). E. coli spp. and Salmonella spp. isolates were further examined by API20and PCR techniques. Antibiogram testing against E. coli spp. and 12 Salmonella spp. isolates, revealed multi-drug resistance pattern and high sensitivity to enrofloxacin, nitrofuranation and ofloxacine. Parasitological examination revealed that 35 (37.2%) quails were infected with chewing lice (Phithiraptera spp.). Intestinal infection with Heterakis spp. and/or Ascarida spp. Was detected in 27 (28.7%) quails. All quails were negative for avian influenza virus by direct detection by Rt-PCR for oral and cloaca swabs followed by inoculation in embryonated chicken eggs. In conclusion, the migratory quails might play a potential role in dispersion of zoonosis in Egypt.
Journal of Veterinary Advances, 2015
RVF virus is a member of the genus Phlebovirus, belongs to the Bunyaviridea family, RVFV is an en... more RVF virus is a member of the genus Phlebovirus, belongs to the Bunyaviridea family, RVFV is an enveloped virus with a diameter of 90 to 110 nm and a core element of 80 to 85 nm. The viral genome consists of single-stranded, segmented tripartited RNA, among which the large (L) and medium (M) segments are negative polarity, and the small (S) segment is ambisense polarity. The RNA of S segment of ZH501VSVRI was extracted then it converts to cDNA and ampilified by Conventional PCR. Electrophoresis of PCR product was done. The DNA band was sliced from the gel and purified followed by sequencing. The resultant sequence was aligned with those of previously characterized RVF viruses by using Bioedit program which revealed three nucleotide substitutions and these lead to presence of three mutations in translating amino acid in non-structure S protein (Ns S).
Veterinary World, 2021
Background and Aim: Poultry infections with H9N2 avian influenza viruses (AIVs) are endemic in Eg... more Background and Aim: Poultry infections with H9N2 avian influenza viruses (AIVs) are endemic in Egypt. This study determined the genetic changes in the sequences of H9N2 AIVs isolated from chicken and quails in Egypt, including determining genetic reassortment and detecting the main genetic changes in hemagglutinin (HA) and neuraminidase (NA) genes. Materials and Methods: Swab samples were collected from chicken and quails, examined through reverse transcription-polymerase chain reaction, and AIVs from positive samples were isolated in embryonated chicken eggs. Complete genome sequencing and phylogenetic analyses were conducted for two H9N2 AIV isolates, and sequences of HA and NA gene segments were analyzed in another two isolates. Results: A novel reassortant virus was identified from a commercial chicken flock (A/chicken/Egypt/374V/2016) and quails from a live bird market (A/quail/Egypt/1253V/2016). The reassortant viruses acquired four genome segments from the classic Egyptian H9...
Tropical Medicine and Health, 2014
Ochratoxin A (OTA) is a serious problem affecting the poultry industry as well as a health hazard... more Ochratoxin A (OTA) is a serious problem affecting the poultry industry as well as a health hazard for humans consuming contaminated poultry meat. The aim of this study was to determine OTA levels in the feed used in some poultry farms in Ismailia, Egypt and in organs of birds from those farms. Commercial ELISA kits were used to detect levels of OTA in poultry feed samples and main feed ingredients as well as its residues in bird's tissues (Kidney, liver and muscles). Poultry feed samples were also tested for mycological contaminations. The most common commercial toxin binders were also evaluated in vitro. OTA concentration in poultry feed and ingredients ranged from 7.10-20.72 µg/kg. Soybean meal samples showed the lowest concentration, while rations formulated within the farm had the highest mean concentration. Producers using different toxin binders had a significant lower OTA concentration in their rations (p<0.05) as compared to those not using binders. OTA residues in different tissues were high in kidney and liver as compared to muscles. Asprigillus flavus was isolated from 80% of the examined feed samples. Different binders showed different abilities to bind OTA in vitro with highest binding capacity for yeast as compared to other commercially available binders.
Intervirology, 2009
... Adel El-Hamid Ahmed Serwah d Yoshitake Hayashi a ... Am J Trop Med Hyg 2000; 63: 236241. 11 ... more ... Adel El-Hamid Ahmed Serwah d Yoshitake Hayashi a ... Am J Trop Med Hyg 2000; 63: 236241. 11 Darwish MA, Faris R, Clemens JD, Rao MR, Edelman R: High seroprevalence of hepatitis A, B, C, and E viruses in residents in an Egyptian village in the Nile Delta: a pilot study. ...