PRADEEP SHELKE | SANT GADGEBABA AMRAVATI UNIVERSITY (original) (raw)
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This review describes brief introduction to the methods of quantitative analysis which are one of... more This review describes brief introduction to the methods of quantitative analysis which are one of
the most valuable course in scientific training. An introduction to HPLC such as stationary phase
used, applications of HPLC is briefly overviewed. The basic principle of mass spectrometry is
shortly discussed. The main objective of this review is to discuss well known and most widely used
hyphenation technique, liquid chromatography in conjunction with mass spectrometry [LC-MS].
Interface plays an important role in the hyphenation technique; as eluent from liquid
chromatography is transferred to mass spectrometer with the help of interface. The development
and type of interfaces used in this hyphenation technique are thoroughly discussed. The mass
analyzers such as Quadrupole, TOF used in mass spectrometry with principle and working are
also discussed.
A selective and sensitive reverse phase high performance liquid chromatographic (RP-HPLC) method ... more A selective and sensitive reverse phase high performance liquid chromatographic (RP-HPLC) method has been
developed for the separation and quantification of metformin HCl (MET) and vildagliptin (VILD) in tablet
dosage form. The determination was carried out using phenomenax C18 column (4.6´150 mm) as a stationary
phase and mobile phase comprised of phosphate buffer (pH6.0): methanol (65:35v/v). The pH of phosphate
buffer is adjusted to 6.0 by using orthophosphoric acid. The flow rate was maintained at 1.0ml/min and the
eluent was monitored at 255nm.The retention time of MET and VILD were 1.503 min and 5.530 min
respectively. The method was validated in terms of linearity, precision, accuracy, ruggedness, specificity and
robustness. The method was linear over the range 50-150 μg/ml for both MET (r = 0.999) and VILD (0.998).
For precision studies; RSD for MET and VILD were 0.24 and 0.14 respectively. The percentage recoveries for
both drugs from their tablets were 100.16 and 99.98 respectively. Inter-day; intra-day RSD for both drugs were
found be 0.27 and 0.26, 0.13 and 0.29 respectively.
This review describes brief introduction to the methods of quantitative analysis which are one of... more This review describes brief introduction to the methods of quantitative analysis which are one of
the most valuable course in scientific training. An introduction to HPLC such as stationary phase
used, applications of HPLC is briefly overviewed. The basic principle of mass spectrometry is
shortly discussed. The main objective of this review is to discuss well known and most widely used
hyphenation technique, liquid chromatography in conjunction with mass spectrometry [LC-MS].
Interface plays an important role in the hyphenation technique; as eluent from liquid
chromatography is transferred to mass spectrometer with the help of interface. The development
and type of interfaces used in this hyphenation technique are thoroughly discussed. The mass
analyzers such as Quadrupole, TOF used in mass spectrometry with principle and working are
also discussed.
A selective and sensitive reverse phase high performance liquid chromatographic (RP-HPLC) method ... more A selective and sensitive reverse phase high performance liquid chromatographic (RP-HPLC) method has been
developed for the separation and quantification of metformin HCl (MET) and vildagliptin (VILD) in tablet
dosage form. The determination was carried out using phenomenax C18 column (4.6´150 mm) as a stationary
phase and mobile phase comprised of phosphate buffer (pH6.0): methanol (65:35v/v). The pH of phosphate
buffer is adjusted to 6.0 by using orthophosphoric acid. The flow rate was maintained at 1.0ml/min and the
eluent was monitored at 255nm.The retention time of MET and VILD were 1.503 min and 5.530 min
respectively. The method was validated in terms of linearity, precision, accuracy, ruggedness, specificity and
robustness. The method was linear over the range 50-150 μg/ml for both MET (r = 0.999) and VILD (0.998).
For precision studies; RSD for MET and VILD were 0.24 and 0.14 respectively. The percentage recoveries for
both drugs from their tablets were 100.16 and 99.98 respectively. Inter-day; intra-day RSD for both drugs were
found be 0.27 and 0.26, 0.13 and 0.29 respectively.