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Research paper thumbnail of The posterior glottis: structural and clinical considerations

European Archives of Oto-Rhino-Laryngology, 2012

Despite the presence of a number of good publications during the past two decades, the posterior ... more Despite the presence of a number of good publications during the past two decades, the posterior glottis (PG) is still not clearly appreciated in clinical laryngological practice. This study focuses on searching the literature to find out the present day awareness of the PG, as well as highlighting some of its clinical features. The investigation proceeds with two main sections: (1) critical analysis of the literature on the PG during the last 50 years (2) describing the clinical appearance and behavior of the PG in 100 normal subjects. Results of section 1 illustrate the limited awareness in the literature of the existence of PG as a distinct part of the human larynx. Results of section 2 illustrate some misconcepts related to the PG as inter-arytenoid pachydermia, change of voice with lesions in the PG. Discussion elaborates on the significance of the findings and attempts to clarify certain misconcepts about the PG, its structure, function, and clinical features. In the light of the data collected from the literature regarding the dimensions of the PG, a set of recommendations were proposed regarding the optimal sizes of the endotracheal tubes in both sexes.

Research paper thumbnail of MRI of Pituitary Macroadenomas: Criteria of Cavernous Sinus Spaces Extension

Research paper thumbnail of Front & Back Matter

ORL, 2012

Each paper needs an abstract in English of not more than 200 words. The abstract is of utmost imp... more Each paper needs an abstract in English of not more than 200 words. The abstract is of utmost importance. It should contain the following information: purpose of the study, procedures, results, conclusions and message of the paper. Footnotes: Avoid footnotes. Tables and illustrations: Tables and illustrations (both numbered in Arabic numerals) should be stored in separate files. Tables require a heading and figures a legend, also in a separate file. When possible, group several illustrations in one block for reproduction (max. size 180  223 mm) or provide crop marks. B/w half-tone and color illustrations must have a final resolution of 300 dpi after scaling, line drawings one of 800–1200 dpi. Color illustrations Online edition: Color illustrations are reproduced free of charge. In the print version, the illustrations are reproduced in black and white. Please avoid referring to the colors in the text and figure legends. Print edition: Up to 6 color illustrations per page can be inte...

Research paper thumbnail of Contents Vol. 75, 2013

ORL, 2014

Access to full text and tables of contents, including tentative ones for forthcoming issues: www.... more Access to full text and tables of contents, including tentative ones for forthcoming issues: www.karger.com/orl_issues 68 Comparison of Ventilation and Voice Outcomes between Unilateral Laryngeal Pacing and Unilateral Cordotomy for the Treatment of Bilateral Vocal Fold Paralysis

Research paper thumbnail of Benign Paroxysmal Positional Vertigo of Lateral Semicircular Canal: A Systematic Review and Meta-Analysis

Advances in Otolaryngology, 2015

Benign paroxysmal positional vertigo (BPPV) is the most common cause of vertigo of peripheral ori... more Benign paroxysmal positional vertigo (BPPV) is the most common cause of vertigo of peripheral origin. The lateral semicircular canal (LSCC) follows the posterior semicircular canal (PSCC) as the site of pathology in the majority of patients. Therapy, aiming at relocating particles causing aberrant LSCC stimulation has been applied by forced prolonged positioning, barbecue, and particle repositioning maneuvers. Results of the different techniques are variable. This systematic review/meta-analysis aimed to find out which therapy technique yields higher cure rates. MedLine database provided at National Library of Medicine was searched for randomized controlled trials comparing results of different therapeutic techniques for patients with LSCC BPPV. For studies included in qualitative analysis/synthesis, the following were collected independently by the author: number of participants, count of patients with geotropic and apogeotropic LSCC in each treatment group, and resolution of verti...

Research paper thumbnail of Human Papilloma Virus E6 RNA in Benign and Malignant Laryngeal Lesions

Research paper thumbnail of Evaluation of Vestibular Function in Patients with Chronic Suppurative Otitis Media

ORL, 2013

Chronic suppurative otitis media can be associated with functional damage to the inner ear result... more Chronic suppurative otitis media can be associated with functional damage to the inner ear resulting in sensorineural hearing loss (SNHL) and dizziness. The proximity of anatomical structures of the inner ear and possibility of damage by the same pathological factor implies that cochlear damage may be accompanied by vestibular damage

Research paper thumbnail of Targeting vascular cross compression of cranial nerves by MRI: Comparing different techniques for the diagnosis, characterization, and identification of prognostic factors

Research paper thumbnail of Vertigo in CSOM tables

Research paper thumbnail of Can vaccination against pneumococci prevent otitis media with effusion?

European Archives of Oto-Rhino-Laryngology, 2012

The most common cause of hearing loss in early childhood is otitis media with effusion (OME). Pre... more The most common cause of hearing loss in early childhood is otitis media with effusion (OME). Prevention of OME in preschool children will improve quality of life. The authors aimed to determine, by the best available published evidence, whether vaccination against pneumococci effectively prevents OME. The study design was based on systematic review (SR) of randomized controlled trials (Level 1a evidence). The medical literature available through searching Medline database was reviewed using the following keywords ''Otitis media with effusion,'' ''secretory otitis media,'' or ''glue ear,'' and ''vaccination'' limiting the search to randomized controlled trials (RCTs) conducted on children (0-18 years), published in English, in the last 10 years. Results of effects of vaccination on prevention of OME from the included RCTs were utilized to conduct a meta-analysis to find out the preventive value of antipneumococcal vaccination. Three RCTs were identified conforming to the inclusion criteria. Meta-analysis of results showed no significant preventive advantage for antipneumococcal vaccination. Based on the results it was confirmed that neither primary nor secondary prevention by antipneumococcal vaccination has a beneficial impact on OME. More RCTs should be conducted to study the effect of vaccination on OME. Level of evidence: 1a, Systematic Review of Randomized Controlled Trials.

Research paper thumbnail of The role of CT scan in the diagnosis of otosclerosis

The Medical journal of Cairo University

Research paper thumbnail of Vertigo in CSOM tables

Research paper thumbnail of Targeting vascular cross compression of cranial nerves by MRI: Comparing different techniques for the diagnosis, characterization, and identification of prognostic factors

Research paper thumbnail of The adjunctive role of application of Mitomycin C intraoperatively with posterior transverse laser cordotomy in treatment of bilateral abductor vocal fold paralysis

Research paper thumbnail of Evaluation of Vestibular Function in Patients with Chronic Suppurative Otitis Media

ORL, 2013

Chronic suppurative otitis media can be associated with functional damage to the inner ear result... more Chronic suppurative otitis media can be associated with functional damage to the inner ear resulting in sensorineural hearing loss (SNHL) and dizziness. The proximity of anatomical structures of the inner ear and possibility of damage by the same pathological factor implies that cochlear damage may be accompanied by vestibular damage

Research paper thumbnail of Animal Model for Training and Improvement of the Surgical Skills in Endolaryngeal Microsurgery

Journal of Voice, 2012

Animal models for training of surgical skills were widely used for a long time in the education o... more Animal models for training of surgical skills were widely used for a long time in the education of medical practitioners. It is recognized, however, that endolaryngeal microsurgery requires highly refined skills to handle the delicate structures of the vocal folds under the microscope. The availability of fresh human laryngeal specimens is markedly restricted by legal and hygienic issues. The aim of this work was to report on the design of a feasible and effective model to provide the much needed skills in an animal laryngeal model that is as close as possible to the human vocal fold structure. In the initial phase of the research, three animal larynges were studied: porcine/pig, bovine/calf, and ovine/sheep larynges. The pig/porcine larynx was chosen for this experimental training model because it closely resembled the human laryngeal/glottal configurations. A study was carried out on 10 porcine/pig larynges to assess the dimensions of the glottis and study the histology of the layered structure of the vocal fold. The study was pursued to confirm the resemblance of this animal specimen to the human vocal fold. A wooden box with a black finished interior was prepared with an acrylic bed at its floor. This bed allows placement of the porcine/pig larynx. The design of the box allows the endoscopic exposure of the porcine/pig larynx through a rubber diaphragm. The darkness and confinement of the box, apart from the light of the endoscope, approximates the situation in live endoscopy. The operating microscope is then used to expose the glottis. Routine fine microlaryngeal instruments were used for training in the prescribed skills.

Research paper thumbnail of Can vaccination against pneumococci prevent otitis media with effusion?

European Archives of Oto-Rhino-Laryngology, 2012

The most common cause of hearing loss in early childhood is otitis media with effusion (OME). Pre... more The most common cause of hearing loss in early childhood is otitis media with effusion (OME). Prevention of OME in preschool children will improve quality of life. The authors aimed to determine, by the best available published evidence, whether vaccination against pneumococci effectively prevents OME. The study design was based on systematic review (SR) of randomized controlled trials (Level 1a evidence). The medical literature available through searching Medline database was reviewed using the following keywords ''Otitis media with effusion,'' ''secretory otitis media,'' or ''glue ear,'' and ''vaccination'' limiting the search to randomized controlled trials (RCTs) conducted on children (0-18 years), published in English, in the last 10 years. Results of effects of vaccination on prevention of OME from the included RCTs were utilized to conduct a meta-analysis to find out the preventive value of antipneumococcal vaccination. Three RCTs were identified conforming to the inclusion criteria. Meta-analysis of results showed no significant preventive advantage for antipneumococcal vaccination. Based on the results it was confirmed that neither primary nor secondary prevention by antipneumococcal vaccination has a beneficial impact on OME. More RCTs should be conducted to study the effect of vaccination on OME. Level of evidence: 1a, Systematic Review of Randomized Controlled Trials.

Research paper thumbnail of Gastroesophageal reflux/laryngopharyngeal reflux disease: a critical analysis of the literature

European Archives of Oto-Rhino-Laryngology, 2010

Despite the wealth of publications on the topic of gastroesophageal reflux and its variants, ther... more Despite the wealth of publications on the topic of gastroesophageal reflux and its variants, there are still many unsettled questions before one accepts the prevalent cult of ''reflux disease''. This study is summarizing the results of the critical analysis of the literature, 436 articles, during the last 30 years. The golden test to identify the patient group suffering from this rather common phenomenon is still lacking. The claimed extra-esophageal manifestations especially in the larynx are non-specific and may be caused by other factors well-known within the domain of vocology. The response to therapeutic intervention still lacks serious well-controlled studies to allow drawing reasonable conclusions. An outstanding feature of the publications is that most of them fall in the category of ''review''. It seems that there is a tendency to perpetuate the concept without objective criticism. Following the analysis, a recommendation for a new plan of original well-controlled multi-center studies is highlighted.

Research paper thumbnail of Congenital auditory meatal atresia: a numerical review

European Archives of Oto-Rhino-Laryngology, 2009

Congenital auditory meatal atresia (CAMA) is an uncommonly encountered disorder. Though a rare co... more Congenital auditory meatal atresia (CAMA) is an uncommonly encountered disorder. Though a rare condition, CAMA poses multiple problems for aVected children. Recent management of CAMA in developed countries rests on osseo-integrated prostheses and boneanchored hearing aids. The situation is diVerent in developing countries where aesthetic and otologic surgeries are the available lines of management. Surgical management of CAMA has been staged into reconstructive surgery for auricular deformity followed by external and middle ear reconstruction either via anterior or transmastoid approaches. Multiple case series describing the outcomes and complications of both approaches have been published, but no authors have attempted to compare either. We have attempted to compare the outcomes and complications of both approaches by analyzing published medical articles concerning surgical management of CAMA identiWed by searching the Medline database using "congenital aural atresia" and "external auditory canal atresia" as keywords. A total of 923 ears were reported by 13 articles included in this study. The transmastoid approach is shown, by multiple linear regression, to have better postoperative hearing gain and less likely restenosis.

Research paper thumbnail of Prognostic value of telomerase and DNA ploidy in laryngeal carcinoma

European Archives of Oto-Rhino-Laryngology, 2005

Prognosis of carcinoma of the larynx is currently based on the morphologic analysis of the tumor ... more Prognosis of carcinoma of the larynx is currently based on the morphologic analysis of the tumor extent, differentiation grading and type of microscopic invasion. The DNA ploidy status and the cell proliferation activity may give complementary information about the prognosis. Telomerase may be used as a measure of tumor proliferation. The aim of this work was to investigate the probable application of telomerase as a measure of cell immortalization as well as DNA ploidy as a measure of cell proliferation in the prognosis of patients with laryngeal carcinoma. The work was carried out as a prospective observational study that included 35 patients with laryngeal carcinoma who were treated surgically at Ain Shams University Hospitals, Cairo, Egypt. Flowcytometric analysis and biochemical assay of telomerase using human Telomerase Reverse Transcriptase (hTERT) mRNA assay by RT-PCR in resected specimens were done. The proliferative indices in different parts of the tumor were found to be significantly different from each other, with those of tumor edges being the highest. The ploidy status at the edge of the tumor was significantly different between surviving and non-surviving patients, while the tumor core proliferative index was found to be significantly lower in patients without recurrence than those with recurrence.

Research paper thumbnail of The posterior glottis: structural and clinical considerations

European Archives of Oto-Rhino-Laryngology, 2012

Despite the presence of a number of good publications during the past two decades, the posterior ... more Despite the presence of a number of good publications during the past two decades, the posterior glottis (PG) is still not clearly appreciated in clinical laryngological practice. This study focuses on searching the literature to find out the present day awareness of the PG, as well as highlighting some of its clinical features. The investigation proceeds with two main sections: (1) critical analysis of the literature on the PG during the last 50 years (2) describing the clinical appearance and behavior of the PG in 100 normal subjects. Results of section 1 illustrate the limited awareness in the literature of the existence of PG as a distinct part of the human larynx. Results of section 2 illustrate some misconcepts related to the PG as inter-arytenoid pachydermia, change of voice with lesions in the PG. Discussion elaborates on the significance of the findings and attempts to clarify certain misconcepts about the PG, its structure, function, and clinical features. In the light of the data collected from the literature regarding the dimensions of the PG, a set of recommendations were proposed regarding the optimal sizes of the endotracheal tubes in both sexes.

Research paper thumbnail of MRI of Pituitary Macroadenomas: Criteria of Cavernous Sinus Spaces Extension

Research paper thumbnail of Front & Back Matter

ORL, 2012

Each paper needs an abstract in English of not more than 200 words. The abstract is of utmost imp... more Each paper needs an abstract in English of not more than 200 words. The abstract is of utmost importance. It should contain the following information: purpose of the study, procedures, results, conclusions and message of the paper. Footnotes: Avoid footnotes. Tables and illustrations: Tables and illustrations (both numbered in Arabic numerals) should be stored in separate files. Tables require a heading and figures a legend, also in a separate file. When possible, group several illustrations in one block for reproduction (max. size 180  223 mm) or provide crop marks. B/w half-tone and color illustrations must have a final resolution of 300 dpi after scaling, line drawings one of 800–1200 dpi. Color illustrations Online edition: Color illustrations are reproduced free of charge. In the print version, the illustrations are reproduced in black and white. Please avoid referring to the colors in the text and figure legends. Print edition: Up to 6 color illustrations per page can be inte...

Research paper thumbnail of Contents Vol. 75, 2013

ORL, 2014

Access to full text and tables of contents, including tentative ones for forthcoming issues: www.... more Access to full text and tables of contents, including tentative ones for forthcoming issues: www.karger.com/orl_issues 68 Comparison of Ventilation and Voice Outcomes between Unilateral Laryngeal Pacing and Unilateral Cordotomy for the Treatment of Bilateral Vocal Fold Paralysis

Research paper thumbnail of Benign Paroxysmal Positional Vertigo of Lateral Semicircular Canal: A Systematic Review and Meta-Analysis

Advances in Otolaryngology, 2015

Benign paroxysmal positional vertigo (BPPV) is the most common cause of vertigo of peripheral ori... more Benign paroxysmal positional vertigo (BPPV) is the most common cause of vertigo of peripheral origin. The lateral semicircular canal (LSCC) follows the posterior semicircular canal (PSCC) as the site of pathology in the majority of patients. Therapy, aiming at relocating particles causing aberrant LSCC stimulation has been applied by forced prolonged positioning, barbecue, and particle repositioning maneuvers. Results of the different techniques are variable. This systematic review/meta-analysis aimed to find out which therapy technique yields higher cure rates. MedLine database provided at National Library of Medicine was searched for randomized controlled trials comparing results of different therapeutic techniques for patients with LSCC BPPV. For studies included in qualitative analysis/synthesis, the following were collected independently by the author: number of participants, count of patients with geotropic and apogeotropic LSCC in each treatment group, and resolution of verti...

Research paper thumbnail of Human Papilloma Virus E6 RNA in Benign and Malignant Laryngeal Lesions

Research paper thumbnail of Evaluation of Vestibular Function in Patients with Chronic Suppurative Otitis Media

ORL, 2013

Chronic suppurative otitis media can be associated with functional damage to the inner ear result... more Chronic suppurative otitis media can be associated with functional damage to the inner ear resulting in sensorineural hearing loss (SNHL) and dizziness. The proximity of anatomical structures of the inner ear and possibility of damage by the same pathological factor implies that cochlear damage may be accompanied by vestibular damage

Research paper thumbnail of Targeting vascular cross compression of cranial nerves by MRI: Comparing different techniques for the diagnosis, characterization, and identification of prognostic factors

Research paper thumbnail of Vertigo in CSOM tables

Research paper thumbnail of Can vaccination against pneumococci prevent otitis media with effusion?

European Archives of Oto-Rhino-Laryngology, 2012

The most common cause of hearing loss in early childhood is otitis media with effusion (OME). Pre... more The most common cause of hearing loss in early childhood is otitis media with effusion (OME). Prevention of OME in preschool children will improve quality of life. The authors aimed to determine, by the best available published evidence, whether vaccination against pneumococci effectively prevents OME. The study design was based on systematic review (SR) of randomized controlled trials (Level 1a evidence). The medical literature available through searching Medline database was reviewed using the following keywords ''Otitis media with effusion,'' ''secretory otitis media,'' or ''glue ear,'' and ''vaccination'' limiting the search to randomized controlled trials (RCTs) conducted on children (0-18 years), published in English, in the last 10 years. Results of effects of vaccination on prevention of OME from the included RCTs were utilized to conduct a meta-analysis to find out the preventive value of antipneumococcal vaccination. Three RCTs were identified conforming to the inclusion criteria. Meta-analysis of results showed no significant preventive advantage for antipneumococcal vaccination. Based on the results it was confirmed that neither primary nor secondary prevention by antipneumococcal vaccination has a beneficial impact on OME. More RCTs should be conducted to study the effect of vaccination on OME. Level of evidence: 1a, Systematic Review of Randomized Controlled Trials.

Research paper thumbnail of The role of CT scan in the diagnosis of otosclerosis

The Medical journal of Cairo University

Research paper thumbnail of Vertigo in CSOM tables

Research paper thumbnail of Targeting vascular cross compression of cranial nerves by MRI: Comparing different techniques for the diagnosis, characterization, and identification of prognostic factors

Research paper thumbnail of The adjunctive role of application of Mitomycin C intraoperatively with posterior transverse laser cordotomy in treatment of bilateral abductor vocal fold paralysis

Research paper thumbnail of Evaluation of Vestibular Function in Patients with Chronic Suppurative Otitis Media

ORL, 2013

Chronic suppurative otitis media can be associated with functional damage to the inner ear result... more Chronic suppurative otitis media can be associated with functional damage to the inner ear resulting in sensorineural hearing loss (SNHL) and dizziness. The proximity of anatomical structures of the inner ear and possibility of damage by the same pathological factor implies that cochlear damage may be accompanied by vestibular damage

Research paper thumbnail of Animal Model for Training and Improvement of the Surgical Skills in Endolaryngeal Microsurgery

Journal of Voice, 2012

Animal models for training of surgical skills were widely used for a long time in the education o... more Animal models for training of surgical skills were widely used for a long time in the education of medical practitioners. It is recognized, however, that endolaryngeal microsurgery requires highly refined skills to handle the delicate structures of the vocal folds under the microscope. The availability of fresh human laryngeal specimens is markedly restricted by legal and hygienic issues. The aim of this work was to report on the design of a feasible and effective model to provide the much needed skills in an animal laryngeal model that is as close as possible to the human vocal fold structure. In the initial phase of the research, three animal larynges were studied: porcine/pig, bovine/calf, and ovine/sheep larynges. The pig/porcine larynx was chosen for this experimental training model because it closely resembled the human laryngeal/glottal configurations. A study was carried out on 10 porcine/pig larynges to assess the dimensions of the glottis and study the histology of the layered structure of the vocal fold. The study was pursued to confirm the resemblance of this animal specimen to the human vocal fold. A wooden box with a black finished interior was prepared with an acrylic bed at its floor. This bed allows placement of the porcine/pig larynx. The design of the box allows the endoscopic exposure of the porcine/pig larynx through a rubber diaphragm. The darkness and confinement of the box, apart from the light of the endoscope, approximates the situation in live endoscopy. The operating microscope is then used to expose the glottis. Routine fine microlaryngeal instruments were used for training in the prescribed skills.

Research paper thumbnail of Can vaccination against pneumococci prevent otitis media with effusion?

European Archives of Oto-Rhino-Laryngology, 2012

The most common cause of hearing loss in early childhood is otitis media with effusion (OME). Pre... more The most common cause of hearing loss in early childhood is otitis media with effusion (OME). Prevention of OME in preschool children will improve quality of life. The authors aimed to determine, by the best available published evidence, whether vaccination against pneumococci effectively prevents OME. The study design was based on systematic review (SR) of randomized controlled trials (Level 1a evidence). The medical literature available through searching Medline database was reviewed using the following keywords ''Otitis media with effusion,'' ''secretory otitis media,'' or ''glue ear,'' and ''vaccination'' limiting the search to randomized controlled trials (RCTs) conducted on children (0-18 years), published in English, in the last 10 years. Results of effects of vaccination on prevention of OME from the included RCTs were utilized to conduct a meta-analysis to find out the preventive value of antipneumococcal vaccination. Three RCTs were identified conforming to the inclusion criteria. Meta-analysis of results showed no significant preventive advantage for antipneumococcal vaccination. Based on the results it was confirmed that neither primary nor secondary prevention by antipneumococcal vaccination has a beneficial impact on OME. More RCTs should be conducted to study the effect of vaccination on OME. Level of evidence: 1a, Systematic Review of Randomized Controlled Trials.

Research paper thumbnail of Gastroesophageal reflux/laryngopharyngeal reflux disease: a critical analysis of the literature

European Archives of Oto-Rhino-Laryngology, 2010

Despite the wealth of publications on the topic of gastroesophageal reflux and its variants, ther... more Despite the wealth of publications on the topic of gastroesophageal reflux and its variants, there are still many unsettled questions before one accepts the prevalent cult of ''reflux disease''. This study is summarizing the results of the critical analysis of the literature, 436 articles, during the last 30 years. The golden test to identify the patient group suffering from this rather common phenomenon is still lacking. The claimed extra-esophageal manifestations especially in the larynx are non-specific and may be caused by other factors well-known within the domain of vocology. The response to therapeutic intervention still lacks serious well-controlled studies to allow drawing reasonable conclusions. An outstanding feature of the publications is that most of them fall in the category of ''review''. It seems that there is a tendency to perpetuate the concept without objective criticism. Following the analysis, a recommendation for a new plan of original well-controlled multi-center studies is highlighted.

Research paper thumbnail of Congenital auditory meatal atresia: a numerical review

European Archives of Oto-Rhino-Laryngology, 2009

Congenital auditory meatal atresia (CAMA) is an uncommonly encountered disorder. Though a rare co... more Congenital auditory meatal atresia (CAMA) is an uncommonly encountered disorder. Though a rare condition, CAMA poses multiple problems for aVected children. Recent management of CAMA in developed countries rests on osseo-integrated prostheses and boneanchored hearing aids. The situation is diVerent in developing countries where aesthetic and otologic surgeries are the available lines of management. Surgical management of CAMA has been staged into reconstructive surgery for auricular deformity followed by external and middle ear reconstruction either via anterior or transmastoid approaches. Multiple case series describing the outcomes and complications of both approaches have been published, but no authors have attempted to compare either. We have attempted to compare the outcomes and complications of both approaches by analyzing published medical articles concerning surgical management of CAMA identiWed by searching the Medline database using "congenital aural atresia" and "external auditory canal atresia" as keywords. A total of 923 ears were reported by 13 articles included in this study. The transmastoid approach is shown, by multiple linear regression, to have better postoperative hearing gain and less likely restenosis.

Research paper thumbnail of Prognostic value of telomerase and DNA ploidy in laryngeal carcinoma

European Archives of Oto-Rhino-Laryngology, 2005

Prognosis of carcinoma of the larynx is currently based on the morphologic analysis of the tumor ... more Prognosis of carcinoma of the larynx is currently based on the morphologic analysis of the tumor extent, differentiation grading and type of microscopic invasion. The DNA ploidy status and the cell proliferation activity may give complementary information about the prognosis. Telomerase may be used as a measure of tumor proliferation. The aim of this work was to investigate the probable application of telomerase as a measure of cell immortalization as well as DNA ploidy as a measure of cell proliferation in the prognosis of patients with laryngeal carcinoma. The work was carried out as a prospective observational study that included 35 patients with laryngeal carcinoma who were treated surgically at Ain Shams University Hospitals, Cairo, Egypt. Flowcytometric analysis and biochemical assay of telomerase using human Telomerase Reverse Transcriptase (hTERT) mRNA assay by RT-PCR in resected specimens were done. The proliferative indices in different parts of the tumor were found to be significantly different from each other, with those of tumor edges being the highest. The ploidy status at the edge of the tumor was significantly different between surviving and non-surviving patients, while the tumor core proliferative index was found to be significantly lower in patients without recurrence than those with recurrence.