Marwa Hegazy | Ain Shams University (original) (raw)

Papers by Marwa Hegazy

Cellular and molecular biology (Noisy-le-Grand, France), 2015

Nephro-and hepatotoxicities are important complications in cancer patients undergoing cisplatin (... more Nephro-and hepatotoxicities are important complications in cancer patients undergoing cisplatin (CP) therapy. We aimed to study the protective effect of fenugreek (FG) on CP induced renal and hepatic injuries in rats. Cisplatin intoxication resulted in structural and functional renal and hepatic impairments, which were revealed by massive histopathological changes and elevated kidney and liver function tests. However, it was associated with oxidative stress and lipid peroxidation as evident by increased reactive oxygen species (ROS) and malondialdehyde (MDA) with decreased levels of total antioxidant activity. Cisplatin administration triggered inflammatory responses and apoptosis in rat livers and kidneys as evident by increased expression of pro-inflammatory cytokine, tumor necrosis factor- α (TNF-α) and apoptotic marker p38 mitogen-activated protein kinase (p38 MAPK) as results of overproduction of ROS. FG significantly attenuated the cisplatin-induced biochemical and histopathologi...

Pediatric Blood & Cancer, 2015

Secondary hemophagocytic syndromes might be associated with a variety of infections, as well as g... more Secondary hemophagocytic syndromes might be associated with a variety of infections, as well as genetic, collagen-vascular and autoimmune diseases and malignancies. They often respond to treatment of the underlying disease and/or IVIG, cyclosporin-A and steroids. A 17-month-old male child was admitted to another hospital with complaints and findings like petechial rash, hepatosplenomegaly, anemia, thrombocytopenia and lytic lesions of the parietal bone. A biopsy of the skin was performed and after the diagnosis of Langerhans cell histiocytosis (LCH), DAL-HX-83 was started. But after an inital successful response, a reactivation occured in 6 months. The patient was transferred to our clinic and a pulmonary involvement was also present. LCH III was started, but progression continued and the treatment was switched to LCH-S 98. After the first course, secondary EBV-associated HLH occured and was successfully treated using IVIG, but relapsed after the second course. This time, all of the screening for bacterial and viral infections were negative and the patient was resistant to IVIG. High dose methylprednisolone (HDMP) (30 mg/kg/day for 3 days, 20 mg/kg/day for 2 days, 10 mg/kg/day for 1 day, 5 mg/kg/day for 1 day and 2 mg/kg/day for 3 weeks) was given.

Hematology/Oncology and Stem Cell Therapy, 2015

14q32 rearrangement has been identified as a recurrent hotspot of translocations in multiple myel... more 14q32 rearrangement has been identified as a recurrent hotspot of translocations in multiple myeloma (MM). The Fluorescence Immunophenotyping and Interphase Cytogenetics as a tool for the Investigation of Neoplasms (known as FICTION technique) for evaluation of chromosomal changes in MM. The aim of this work is to detect 14q32 rearrangement, using FICTION technique, on archival bone marrow (BM) slides of MM patients, and to study its prognostic value. This study was conducted at Ain Shams University Hospital. The FICTION technique, which uses CD138 and dual color, and the break-apart 14q32 rearrangement probe, was performed on archived smears of BM slides for 50 MM patients at the time of diagnosis. A significantly higher percentage of cases were positive for 14q32 rearrangement by FICTION (32%) compared to fluorescence in situ hybridization (FISH) (12%) (p=0.04). Cases positive by FICTION for the rearrangement were designated as Group A, while negative cases were designated as Group B. Significantly lower Hb and CRP levels were found among Group B when compared to Group A patients (p=0.001 and 0.01, respectively). Serum albumin level and Bence Jones protein (BJP) significantly affect overall survival (OS) (p=0.01, 0.007, respectively). However, a statistically non-significant shorter mean survival time was found in positive cases through FICTION versus negative cases. FICTION technique provides a sensitive tool for establishing clonal plasma cells (PC) infiltration of BM aspirates, and is amenable for use on archived as well as fresh smears.

Cancer biomarkers : section A of Disease markers, 2007

The purpose of this study was to evaluate the diagnostic efficacy of urinary transforming growth ... more The purpose of this study was to evaluate the diagnostic efficacy of urinary transforming growth factor-beta1 (TGF-beta1) and vascular endothelial growth factor (VEGF) in comparison with voided urine cytology in the detection of bladder cancer. This study included 120 patients with bladder cancer, 54 patients with benign urological disorders and 55 healthy volunteers. Urine supernatant was used for estimation of TGF-beta1 and VEGF by ELISA. VEGF was detected by Western blot (WB) analysis in the urine supernatant of randomly selected bladder cancer patients. The urine sediment was used for cytology. There was a statistically significant difference in the median levels of TGF-beta1 (P=0.002) and VEGF (P=0.000) between the control, benign and malignant groups. The concordance rate of VEGF ELISA with VEGF WB was 96.3%. The overall sensitivity and specificity were 70.8% and 90.8% for voided urine cytology, 71.6% and 59.6% for TGF-beta1, and 76.7% and 61.5% for VEGF. The combined use of v...

Translational Research, 2015

We assessed the differential expression of a urinary panel of microRNAs (miRs) in terms of potent... more We assessed the differential expression of a urinary panel of microRNAs (miRs) in terms of potential application as diagnostic markers of bladder cancer (BC) and relationship to bilharziasis. We investigated voided urine samples and blood from patients with BC (n = 188), benign bladder lesions (n = 88), and age-matched controls (n = 92). Five miRs (miR-210, miR-10b, miR-29c, miR-221, and miR-23a) were selected from previous microarray signature profiling (released by miR2Disease). Afterward, they were validated using polymerase chain reaction array. The expression levels of miR-210, miR-10b, and miR-29c in the urine samples were significantly higher in BC (P < 0.001). The receiver-operating characteristic curve analyses demonstrated that each miR had good sensitivity and specificity for distinguishing patients with BC from patients without BC (miR-210, 71.3% and 91.1%; miR-10b, 80.9% and 91.1%; and miR-183, 71.3% and 88.9%). On combining the 3 miR detection data with the urinary cytology, the results sensitivity increased to 95.2%. Relative quantity mean rank of the miR-29c was significantly higher in the bilharzial-positive patients compared with bilharzial-negative patients. To conclude, urine miR-210, miR-10b, and miR-29c are promising tumor markers for BC: bilharzial and nonbilharzial.

Platelets, 2014

The diagnosis of primary immune thrombocytopenia (ITP) is clinical and cannot be established by a... more The diagnosis of primary immune thrombocytopenia (ITP) is clinical and cannot be established by any specific laboratory assay. Perhaps the best diagnostic study is assessment of the patient's response to ITP therapy. Oxidative stress-related pathways were among the most significant chronic ITP-associated pathways. Overexpression of VNN1 gene, an oxidative stress sensor in epithelial cells, was most strongly associated with progression to chronic ITP. To address this issue, we tested the hypothesis that blood vanin-1 protein level could distinguish between chronic responders and non-responders ITP patients as well as between ITP patients and healthy controls. Vanin-1 protein levels were determined in peripheral blood leukocytes of 80 adult subjects (16 newly diagnosed ITP patients, 24 chronic responders ITP patients, 24 chronic non-responders ITP patients and 16 healthy controls) by enzyme-linked immunesorbent assay (ELISA). Blood vanin-1 protein levels were lower in controls (median = 18.39 ng) than in ITP patients (median = 58.78 ng) with a highly significant p value (p < 0.001). Vanin-1 levels were highly significantly elevated in newly diagnosed ITP patients (median = 188.62 ng) in comparison to chronic responders (median= 26.90 ng) and chronic non-responders (median = 73.87 ng). Vanin-1 level at a cut-off value of…

Clinical Biochemistry, 2012

and sharing with colleagues.

Higher levels of morbidity and mortality are due to malignant tumors and Hepatocellular carcinoma... more Higher levels of morbidity and mortality are due to malignant tumors and Hepatocellular carcinoma (HCC) is considered of a great percentage. The liver-specific microRNA-122 (mir-122) is frequently down-regulated in HCC and is a promising biomarker for the early HCC diagnosis. Restoration of mir-122 renders HCC cells to be sensitive to doxorubicin. The present study aims to investigate the effect of treatment of HCC cells with doxorubicin and/or mir-122 mimics on AFP expression levels. Using HepG2 cells with different treatments, AFP mRNAs and proteins were isolated and detected by real-time PCR and ELISA, respectively. Our data showed that there was a dynamic change in mir-122 expression as compared to inhibitor negative control. Also, there were no significant differences in AFP expression levels in cells treated with chronic doxorubicin dose then transfected with mir-122 mimics. However, our findings demonstrated statistical significant increase in both molecular and protein expression levels of AFP in cells treated with acute high doxorubicin dose and mir-122 mimics. This could be associated with increasing doxorubicin sensitivity by mir-122 restoration in HepG2 cells. This distinct finding could be attributed to a negative feedback increase in AFP levels to counteract the apoptosis/reactive oxygen species triggered by miR-122 restoration in doxorubicin-treated HepG2 cells.

Cellular and molecular biology (Noisy-le-Grand, France), 2015

Nephro-and hepatotoxicities are important complications in cancer patients undergoing cisplatin (... more Nephro-and hepatotoxicities are important complications in cancer patients undergoing cisplatin (CP) therapy. We aimed to study the protective effect of fenugreek (FG) on CP induced renal and hepatic injuries in rats. Cisplatin intoxication resulted in structural and functional renal and hepatic impairments, which were revealed by massive histopathological changes and elevated kidney and liver function tests. However, it was associated with oxidative stress and lipid peroxidation as evident by increased reactive oxygen species (ROS) and malondialdehyde (MDA) with decreased levels of total antioxidant activity. Cisplatin administration triggered inflammatory responses and apoptosis in rat livers and kidneys as evident by increased expression of pro-inflammatory cytokine, tumor necrosis factor- α (TNF-α) and apoptotic marker p38 mitogen-activated protein kinase (p38 MAPK) as results of overproduction of ROS. FG significantly attenuated the cisplatin-induced biochemical and histopathologi...

Pediatric Blood & Cancer, 2015

Secondary hemophagocytic syndromes might be associated with a variety of infections, as well as g... more Secondary hemophagocytic syndromes might be associated with a variety of infections, as well as genetic, collagen-vascular and autoimmune diseases and malignancies. They often respond to treatment of the underlying disease and/or IVIG, cyclosporin-A and steroids. A 17-month-old male child was admitted to another hospital with complaints and findings like petechial rash, hepatosplenomegaly, anemia, thrombocytopenia and lytic lesions of the parietal bone. A biopsy of the skin was performed and after the diagnosis of Langerhans cell histiocytosis (LCH), DAL-HX-83 was started. But after an inital successful response, a reactivation occured in 6 months. The patient was transferred to our clinic and a pulmonary involvement was also present. LCH III was started, but progression continued and the treatment was switched to LCH-S 98. After the first course, secondary EBV-associated HLH occured and was successfully treated using IVIG, but relapsed after the second course. This time, all of the screening for bacterial and viral infections were negative and the patient was resistant to IVIG. High dose methylprednisolone (HDMP) (30 mg/kg/day for 3 days, 20 mg/kg/day for 2 days, 10 mg/kg/day for 1 day, 5 mg/kg/day for 1 day and 2 mg/kg/day for 3 weeks) was given.

Hematology/Oncology and Stem Cell Therapy, 2015

14q32 rearrangement has been identified as a recurrent hotspot of translocations in multiple myel... more 14q32 rearrangement has been identified as a recurrent hotspot of translocations in multiple myeloma (MM). The Fluorescence Immunophenotyping and Interphase Cytogenetics as a tool for the Investigation of Neoplasms (known as FICTION technique) for evaluation of chromosomal changes in MM. The aim of this work is to detect 14q32 rearrangement, using FICTION technique, on archival bone marrow (BM) slides of MM patients, and to study its prognostic value. This study was conducted at Ain Shams University Hospital. The FICTION technique, which uses CD138 and dual color, and the break-apart 14q32 rearrangement probe, was performed on archived smears of BM slides for 50 MM patients at the time of diagnosis. A significantly higher percentage of cases were positive for 14q32 rearrangement by FICTION (32%) compared to fluorescence in situ hybridization (FISH) (12%) (p=0.04). Cases positive by FICTION for the rearrangement were designated as Group A, while negative cases were designated as Group B. Significantly lower Hb and CRP levels were found among Group B when compared to Group A patients (p=0.001 and 0.01, respectively). Serum albumin level and Bence Jones protein (BJP) significantly affect overall survival (OS) (p=0.01, 0.007, respectively). However, a statistically non-significant shorter mean survival time was found in positive cases through FICTION versus negative cases. FICTION technique provides a sensitive tool for establishing clonal plasma cells (PC) infiltration of BM aspirates, and is amenable for use on archived as well as fresh smears.

Cancer biomarkers : section A of Disease markers, 2007

The purpose of this study was to evaluate the diagnostic efficacy of urinary transforming growth ... more The purpose of this study was to evaluate the diagnostic efficacy of urinary transforming growth factor-beta1 (TGF-beta1) and vascular endothelial growth factor (VEGF) in comparison with voided urine cytology in the detection of bladder cancer. This study included 120 patients with bladder cancer, 54 patients with benign urological disorders and 55 healthy volunteers. Urine supernatant was used for estimation of TGF-beta1 and VEGF by ELISA. VEGF was detected by Western blot (WB) analysis in the urine supernatant of randomly selected bladder cancer patients. The urine sediment was used for cytology. There was a statistically significant difference in the median levels of TGF-beta1 (P=0.002) and VEGF (P=0.000) between the control, benign and malignant groups. The concordance rate of VEGF ELISA with VEGF WB was 96.3%. The overall sensitivity and specificity were 70.8% and 90.8% for voided urine cytology, 71.6% and 59.6% for TGF-beta1, and 76.7% and 61.5% for VEGF. The combined use of v...

Translational Research, 2015

We assessed the differential expression of a urinary panel of microRNAs (miRs) in terms of potent... more We assessed the differential expression of a urinary panel of microRNAs (miRs) in terms of potential application as diagnostic markers of bladder cancer (BC) and relationship to bilharziasis. We investigated voided urine samples and blood from patients with BC (n = 188), benign bladder lesions (n = 88), and age-matched controls (n = 92). Five miRs (miR-210, miR-10b, miR-29c, miR-221, and miR-23a) were selected from previous microarray signature profiling (released by miR2Disease). Afterward, they were validated using polymerase chain reaction array. The expression levels of miR-210, miR-10b, and miR-29c in the urine samples were significantly higher in BC (P < 0.001). The receiver-operating characteristic curve analyses demonstrated that each miR had good sensitivity and specificity for distinguishing patients with BC from patients without BC (miR-210, 71.3% and 91.1%; miR-10b, 80.9% and 91.1%; and miR-183, 71.3% and 88.9%). On combining the 3 miR detection data with the urinary cytology, the results sensitivity increased to 95.2%. Relative quantity mean rank of the miR-29c was significantly higher in the bilharzial-positive patients compared with bilharzial-negative patients. To conclude, urine miR-210, miR-10b, and miR-29c are promising tumor markers for BC: bilharzial and nonbilharzial.

Platelets, 2014

The diagnosis of primary immune thrombocytopenia (ITP) is clinical and cannot be established by a... more The diagnosis of primary immune thrombocytopenia (ITP) is clinical and cannot be established by any specific laboratory assay. Perhaps the best diagnostic study is assessment of the patient's response to ITP therapy. Oxidative stress-related pathways were among the most significant chronic ITP-associated pathways. Overexpression of VNN1 gene, an oxidative stress sensor in epithelial cells, was most strongly associated with progression to chronic ITP. To address this issue, we tested the hypothesis that blood vanin-1 protein level could distinguish between chronic responders and non-responders ITP patients as well as between ITP patients and healthy controls. Vanin-1 protein levels were determined in peripheral blood leukocytes of 80 adult subjects (16 newly diagnosed ITP patients, 24 chronic responders ITP patients, 24 chronic non-responders ITP patients and 16 healthy controls) by enzyme-linked immunesorbent assay (ELISA). Blood vanin-1 protein levels were lower in controls (median = 18.39 ng) than in ITP patients (median = 58.78 ng) with a highly significant p value (p < 0.001). Vanin-1 levels were highly significantly elevated in newly diagnosed ITP patients (median = 188.62 ng) in comparison to chronic responders (median= 26.90 ng) and chronic non-responders (median = 73.87 ng). Vanin-1 level at a cut-off value of…

Clinical Biochemistry, 2012

and sharing with colleagues.

Higher levels of morbidity and mortality are due to malignant tumors and Hepatocellular carcinoma... more Higher levels of morbidity and mortality are due to malignant tumors and Hepatocellular carcinoma (HCC) is considered of a great percentage. The liver-specific microRNA-122 (mir-122) is frequently down-regulated in HCC and is a promising biomarker for the early HCC diagnosis. Restoration of mir-122 renders HCC cells to be sensitive to doxorubicin. The present study aims to investigate the effect of treatment of HCC cells with doxorubicin and/or mir-122 mimics on AFP expression levels. Using HepG2 cells with different treatments, AFP mRNAs and proteins were isolated and detected by real-time PCR and ELISA, respectively. Our data showed that there was a dynamic change in mir-122 expression as compared to inhibitor negative control. Also, there were no significant differences in AFP expression levels in cells treated with chronic doxorubicin dose then transfected with mir-122 mimics. However, our findings demonstrated statistical significant increase in both molecular and protein expression levels of AFP in cells treated with acute high doxorubicin dose and mir-122 mimics. This could be associated with increasing doxorubicin sensitivity by mir-122 restoration in HepG2 cells. This distinct finding could be attributed to a negative feedback increase in AFP levels to counteract the apoptosis/reactive oxygen species triggered by miR-122 restoration in doxorubicin-treated HepG2 cells.