Manouchehr Vossoughi | Sharif University of Technology (original) (raw)
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Papers by Manouchehr Vossoughi
A mesophilic iron oxidizing bacterium, Acidithiobacillus ferrooxidans, has been isolated (33 °C) ... more A mesophilic iron oxidizing bacterium, Acidithiobacillus ferrooxidans, has been isolated (33 °C) from a typical chalcopyrite copper concentrate of the Sarcheshmeh copper mine in the region of Kerman located in the south of Iran. In addition, a thermophilic iron oxidizing bacterium, Sulfobacillus, has been isolated (60 °C) from the Kooshk lead and zinc mine near the city of Yazd in
Scientific reports, 2016
As a bi-functional cancer treatment agent, a new hybrid nanostructure is presented which can be u... more As a bi-functional cancer treatment agent, a new hybrid nanostructure is presented which can be used for photothermal therapy by exposure to one order of magnitude lower laser powers compared to similar nanostructures in addition to substantial enhancment in magnetic resonance imaging (MRI) contrast. This gold-iron oxide hybrid nanostructure (GIHN) is synthesized by a cost-effective and high yield water-based approach. The GIHN is sheilded by PEG. Therefore, it shows high hemo and biocompatibility and more than six month stability. Alongside earlier nanostructures, the heat generation rate of GIHN is compareable with surfactnat-capped gold nanorods (GNRs). Two reasons are behind this enhancement: Firstly the distance between GNRs and SPIONs is adjusted in a way that the surface plasmon resonance of the new nanostructure is similar to bare GNRs and secondly the fraction of GNRs is raised in the hybrid nanostructure. GIHN is then applied as a photothermal agent using laser irradiation...
Key Engineering Materials, Sep 6, 2011
Scientific Reports, 2015
High resolution is nearly lost at the expense of throughput in most conventional bioseparation me... more High resolution is nearly lost at the expense of throughput in most conventional bioseparation methods. Nanoparticles, due to their high surface to volume ratio, are attractiveenzyme carriers, which can boost the performance of extraction manifold. Here, wereport design and application ofa method highly capable of improving the partitioning of α-amylase in aqueous two-phase system of polymer and salt. Silica nanoparticle introduced to the system acts as a bridge that connects the enzyme and polymer. Theconjugated nanoparticles form the major part of the upper phase and thus significantly enhance the protein recovery. A thorough investigation was performed on the structure of the nanoconjugatesas well as analyzing the conformational structure of the enzyme after conjugationto explore anypossible denaturation.
PloS one, 2015
Cell culture feeds optimization is a critical step in process development of pharmaceutical recom... more Cell culture feeds optimization is a critical step in process development of pharmaceutical recombinant protein production. Amino acids are the basic supplements of mammalian cell culture feeds with known effect on their growth promotion and productivity. In this study, we reported the implementation of the Plackett-Burman (PB) multifactorial design to screen the effects of amino acids on the growth promotion and productivity of a Chinese hamster ovary DG-44 (CHO-DG44) cell line producing bevacizumab. After this screening, the amino acid combinations were optimized by the response surface methodology (RSM) to determine the most effective concentration in feeds. Through this strategy, the final monoclonal antibody (mAb) titre was enhanced by 70%, compared to the control group. For this particular cell line, aspartic acid, glutamic acid, arginine and glycine had the highest positive effects on the final mAb titre. Simultaneously, the impact of the designed amino acid feed on some crit...
A model-based approach to investigate the effect of two process parameters was proposed for optim... more A model-based approach to investigate the effect of two process parameters was proposed for optimization of the ion-exchange step in the purification of the restriction enzyme PstI .Transport-Dispersive model was used to describe convective and dispersive flow in the column and the proteins transfer to the surface of the resin beads. For protein adsorption step, the well-known Steric Mass Action (SMA) model was coupled with the bulk fluid model. In next step, several experiments designed to estimate model parameters and the resulting model efficiency and ability to predict the elution behavior was tested and verified. Finally, the operating conditions were optimized with changing indicated parameters and study of the objective function (Yield).Gradient slope and feed volume of the sample was selected as the decision variables. Purity was also selected as the constraint and given the value of 99%. The model predicted the yield could become as higher as 52% and this result was validat...
Bioresource Technology, 2015
Journal of Environmental Chemical Engineering
After silica coating of the prepared nickel ferrite nanoparticles, a micrometric layer of zeoliti... more After silica coating of the prepared nickel ferrite nanoparticles, a micrometric layer of zeolitic material (analcime) was grown on the surface. To make the composites good host, surface modification was done with [BMIM]PF6 ionic liquid. Finally, the samples became antibacterial agents via loading the Ag and Au nanoparticles and denoted as X@IL/A/S/NF (X = Ag or Au). All the samples were well characterized by Fourier transform infrared spectroscopy (FTIR), vibrating sample magnetometer (VSM), X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray (EDX), and N2 adsorption–desorption isotherms (BET) analysis methods. Antibacterial activity of the prepared samples was investigated by inactivating Escherichia coli and Streptomyces aureus as the gram negative and gram positive bacterium, respectively. According to the TEM images of cell walls after the treatment time, the antibacterial behavior was discussed. Releasing of noble metal ions from the zeolitic p...
Journal of Molecular Liquids, 2015
Fluid Phase Equilibria, 2015
Applied Catalysis B: Environmental, 2015
ABSTRACT In this study, the antibacterial effect of a Fe and Cd co-doped TiO2 (Fe–Cd/TiO2) visibl... more ABSTRACT In this study, the antibacterial effect of a Fe and Cd co-doped TiO2 (Fe–Cd/TiO2) visible light sensitive photocatalyst was optimized by varying operating parameters and using a response surface methodology to evaluate the experimental data. Twenty sets of disinfection experiments were conducted by adjusting three operating parameters, i.e. initial pH of the solution, reaction temperature and catalyst loading, at five levels. Based on the experimental data, a semi-empirical model was established and subsequently applied to predict the final concentration of Escherichia coli after 45 min exposure to the catalyst and visible light irradiation. Using the accurate model (coefficient of determination R2 = 0.963), optimum values were found to be 40.1 °C, 6.3, and 1.0 g/L for reaction temperature, initial pH of solution and photocatalyst loading, respectively. Under the optimized conditions, the Fe–Cd/TiO2 catalyst achieved a bacterial inactivation efficiency of 99.9% after 45 min of visible light illumination.
Journal of environmental health science & engineering, 2014
A novel implementation of a hybrid membrane bioreactor (HMBR) has been studied in this paper. It ... more A novel implementation of a hybrid membrane bioreactor (HMBR) has been studied in this paper. It is utilized as combination of rotating biological contractor (RBC) and an external membrane, as a new biological system for oily wastewater treatment. Chemical oxygen demand (COD) and total petroleum hydrocarbon (TPH) as factors of Biodegradability has been evaluated. They are both compared together for different hydraulic retention times (HRTs) and petroleum pollution concentrations in RBC and HMBR. The ratio of TPH to COD of Molasses has been varied between 0.2 to 0.8 at two HRTs of 18 and 24 hours while the temperature, pH and dissolved oxygen were kept in the range of 20-25°C, 6.5-7.5, and 2-3.5 mg/l, respectively. The best TPH removal efficiency (99%) was observed in TPH/COD = 0.6 and HRT = 24 hr in HMBR and Removal efficiency was decreased in the ratios above 0.6 in both bioreactors. The experimental results showed that HMBR had higher treatment efficiency than RBC at all ratios an...
Bioresource Technology, 2015
Key Engineering Materials, 2011
A mesophilic iron oxidizing bacterium, Acidithiobacillus ferrooxidans, has been isolated (33 °C) ... more A mesophilic iron oxidizing bacterium, Acidithiobacillus ferrooxidans, has been isolated (33 °C) from a typical chalcopyrite copper concentrate of the Sarcheshmeh copper mine in the region of Kerman located in the south of Iran. In addition, a thermophilic iron oxidizing bacterium, Sulfobacillus, has been isolated (60 °C) from the Kooshk lead and zinc mine near the city of Yazd in
Scientific reports, 2016
As a bi-functional cancer treatment agent, a new hybrid nanostructure is presented which can be u... more As a bi-functional cancer treatment agent, a new hybrid nanostructure is presented which can be used for photothermal therapy by exposure to one order of magnitude lower laser powers compared to similar nanostructures in addition to substantial enhancment in magnetic resonance imaging (MRI) contrast. This gold-iron oxide hybrid nanostructure (GIHN) is synthesized by a cost-effective and high yield water-based approach. The GIHN is sheilded by PEG. Therefore, it shows high hemo and biocompatibility and more than six month stability. Alongside earlier nanostructures, the heat generation rate of GIHN is compareable with surfactnat-capped gold nanorods (GNRs). Two reasons are behind this enhancement: Firstly the distance between GNRs and SPIONs is adjusted in a way that the surface plasmon resonance of the new nanostructure is similar to bare GNRs and secondly the fraction of GNRs is raised in the hybrid nanostructure. GIHN is then applied as a photothermal agent using laser irradiation...
Key Engineering Materials, Sep 6, 2011
Scientific Reports, 2015
High resolution is nearly lost at the expense of throughput in most conventional bioseparation me... more High resolution is nearly lost at the expense of throughput in most conventional bioseparation methods. Nanoparticles, due to their high surface to volume ratio, are attractiveenzyme carriers, which can boost the performance of extraction manifold. Here, wereport design and application ofa method highly capable of improving the partitioning of α-amylase in aqueous two-phase system of polymer and salt. Silica nanoparticle introduced to the system acts as a bridge that connects the enzyme and polymer. Theconjugated nanoparticles form the major part of the upper phase and thus significantly enhance the protein recovery. A thorough investigation was performed on the structure of the nanoconjugatesas well as analyzing the conformational structure of the enzyme after conjugationto explore anypossible denaturation.
PloS one, 2015
Cell culture feeds optimization is a critical step in process development of pharmaceutical recom... more Cell culture feeds optimization is a critical step in process development of pharmaceutical recombinant protein production. Amino acids are the basic supplements of mammalian cell culture feeds with known effect on their growth promotion and productivity. In this study, we reported the implementation of the Plackett-Burman (PB) multifactorial design to screen the effects of amino acids on the growth promotion and productivity of a Chinese hamster ovary DG-44 (CHO-DG44) cell line producing bevacizumab. After this screening, the amino acid combinations were optimized by the response surface methodology (RSM) to determine the most effective concentration in feeds. Through this strategy, the final monoclonal antibody (mAb) titre was enhanced by 70%, compared to the control group. For this particular cell line, aspartic acid, glutamic acid, arginine and glycine had the highest positive effects on the final mAb titre. Simultaneously, the impact of the designed amino acid feed on some crit...
A model-based approach to investigate the effect of two process parameters was proposed for optim... more A model-based approach to investigate the effect of two process parameters was proposed for optimization of the ion-exchange step in the purification of the restriction enzyme PstI .Transport-Dispersive model was used to describe convective and dispersive flow in the column and the proteins transfer to the surface of the resin beads. For protein adsorption step, the well-known Steric Mass Action (SMA) model was coupled with the bulk fluid model. In next step, several experiments designed to estimate model parameters and the resulting model efficiency and ability to predict the elution behavior was tested and verified. Finally, the operating conditions were optimized with changing indicated parameters and study of the objective function (Yield).Gradient slope and feed volume of the sample was selected as the decision variables. Purity was also selected as the constraint and given the value of 99%. The model predicted the yield could become as higher as 52% and this result was validat...
Bioresource Technology, 2015
Journal of Environmental Chemical Engineering
After silica coating of the prepared nickel ferrite nanoparticles, a micrometric layer of zeoliti... more After silica coating of the prepared nickel ferrite nanoparticles, a micrometric layer of zeolitic material (analcime) was grown on the surface. To make the composites good host, surface modification was done with [BMIM]PF6 ionic liquid. Finally, the samples became antibacterial agents via loading the Ag and Au nanoparticles and denoted as X@IL/A/S/NF (X = Ag or Au). All the samples were well characterized by Fourier transform infrared spectroscopy (FTIR), vibrating sample magnetometer (VSM), X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray (EDX), and N2 adsorption–desorption isotherms (BET) analysis methods. Antibacterial activity of the prepared samples was investigated by inactivating Escherichia coli and Streptomyces aureus as the gram negative and gram positive bacterium, respectively. According to the TEM images of cell walls after the treatment time, the antibacterial behavior was discussed. Releasing of noble metal ions from the zeolitic p...
Journal of Molecular Liquids, 2015
Fluid Phase Equilibria, 2015
Applied Catalysis B: Environmental, 2015
ABSTRACT In this study, the antibacterial effect of a Fe and Cd co-doped TiO2 (Fe–Cd/TiO2) visibl... more ABSTRACT In this study, the antibacterial effect of a Fe and Cd co-doped TiO2 (Fe–Cd/TiO2) visible light sensitive photocatalyst was optimized by varying operating parameters and using a response surface methodology to evaluate the experimental data. Twenty sets of disinfection experiments were conducted by adjusting three operating parameters, i.e. initial pH of the solution, reaction temperature and catalyst loading, at five levels. Based on the experimental data, a semi-empirical model was established and subsequently applied to predict the final concentration of Escherichia coli after 45 min exposure to the catalyst and visible light irradiation. Using the accurate model (coefficient of determination R2 = 0.963), optimum values were found to be 40.1 °C, 6.3, and 1.0 g/L for reaction temperature, initial pH of solution and photocatalyst loading, respectively. Under the optimized conditions, the Fe–Cd/TiO2 catalyst achieved a bacterial inactivation efficiency of 99.9% after 45 min of visible light illumination.
Journal of environmental health science & engineering, 2014
A novel implementation of a hybrid membrane bioreactor (HMBR) has been studied in this paper. It ... more A novel implementation of a hybrid membrane bioreactor (HMBR) has been studied in this paper. It is utilized as combination of rotating biological contractor (RBC) and an external membrane, as a new biological system for oily wastewater treatment. Chemical oxygen demand (COD) and total petroleum hydrocarbon (TPH) as factors of Biodegradability has been evaluated. They are both compared together for different hydraulic retention times (HRTs) and petroleum pollution concentrations in RBC and HMBR. The ratio of TPH to COD of Molasses has been varied between 0.2 to 0.8 at two HRTs of 18 and 24 hours while the temperature, pH and dissolved oxygen were kept in the range of 20-25°C, 6.5-7.5, and 2-3.5 mg/l, respectively. The best TPH removal efficiency (99%) was observed in TPH/COD = 0.6 and HRT = 24 hr in HMBR and Removal efficiency was decreased in the ratios above 0.6 in both bioreactors. The experimental results showed that HMBR had higher treatment efficiency than RBC at all ratios an...
Bioresource Technology, 2015
Key Engineering Materials, 2011